Role for Functional SOD2 Polymorphism in Pulmonary Arterial Hypertension in a Chinese Population
Abstract
:1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Patients and Controls
2.3. DNA Extraction and SNP Genotyping
2.4. Construction of SOD2 Expression Plasmid
2.5. Cell Culture and Transient Transfection
2.6. Expression Levels of SOD2 mRNA and Protein
2.7. Statistical Analysis
3. Results
3.1. Characteristics of Study Subjects
3.2. Genotype Frequencies of SOD2 Polymorphism in PAH Cases and Controls
3.3. Correlation with Clinical Features and SOD2 rs4880
3.4. Effect of SOD2 Polymorphism on SOD2 Transcription
4. Discussion
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Variables | Case (n = 460) | Control (n = 530) | p |
---|---|---|---|
age (years) | 44.6 ± 13.9 | 45.4 ± 13.6 | 0.861 |
sex | 0.853 | ||
male | 135 (29.3%) | 163 (30.8%) | |
female | 325 (70.7%) | 367 (69.2%) | |
BMI (kg/m2) | 25.6 ± 4.5 | 25.1 ± 5.3 | 0.399 |
6MWD (m) | 247.9 (146.2–363.3) | ||
NT-proBNP (pg/mL) | 1139.9 (360.9–2471.8) | ||
mPAP (mmHg) | 51 (40–59) | ||
PVRI (dyn*sec*m2/cm5) | 1485.2 (1137.3–2742.7) | ||
CO (L/min) | 3.86 ± 1.81 | ||
CI (L/min/m2) | 2.23 ± 0.88 |
rs4880 T > C | Cases, n (%) | Controls, n (%) | OR (95% CI) a | p a |
---|---|---|---|---|
TT | 319 (69.35%) | 426 (80.37%) | Reference | - |
TC | 111 (24.13%) | 99 (18.68%) | 1.35 (0.79–2.81) | 0.195 |
CC | 30 (6.52%) | 5 (0.94%) | 3.81 (1.22–5.67) | 0.013 |
TC + CC | 141 (30.65%) | 104 (19.62%) | 2.75 (1.23–3.98) | 0.010 |
p trend | 0.023 |
Variables | Case (n = 460) | p Value a | |
---|---|---|---|
TT (n = 319) | TC + CC (n = 141) | ||
6MWD (m) | 258.7 ± 49.6 | 239.2 ± 41.3 | 0.001 |
NT-proBNP (pg/mL) | 1075.7 ± 256.5 | 1177.8 ± 243.4 | <0.001 |
mPAP (mmHg) | 50 ± 5 | 52 ± 7 | <0.001 |
PVRI (dyn*sec*m2/cm5) | 1461.6 ± 252.9 | 1516.0 ± 274.2 | 0.063 b |
CO (L/min) | 4.01 ± 1.84 | 3.58 ± 1.73 | 0.021 |
CI (L/min/m2) | 2.36 ± 0.79 | 2.18 ± 0.91 | 0.035 |
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Xu, M.; Xu, M.; Han, L.; Yuan, C.; Mei, Y.; Zhang, H.; Chen, S.; Sun, K.; Zhu, B. Role for Functional SOD2 Polymorphism in Pulmonary Arterial Hypertension in a Chinese Population. Int. J. Environ. Res. Public Health 2017, 14, 266. https://doi.org/10.3390/ijerph14030266
Xu M, Xu M, Han L, Yuan C, Mei Y, Zhang H, Chen S, Sun K, Zhu B. Role for Functional SOD2 Polymorphism in Pulmonary Arterial Hypertension in a Chinese Population. International Journal of Environmental Research and Public Health. 2017; 14(3):266. https://doi.org/10.3390/ijerph14030266
Chicago/Turabian StyleXu, Ming, Min Xu, Lei Han, Chao Yuan, Yong Mei, Hengdong Zhang, Shi Chen, Kai Sun, and Baoli Zhu. 2017. "Role for Functional SOD2 Polymorphism in Pulmonary Arterial Hypertension in a Chinese Population" International Journal of Environmental Research and Public Health 14, no. 3: 266. https://doi.org/10.3390/ijerph14030266