Self-Powered Electrochemical Lactate Biosensing
AbstractThis work presents the development and characterization of a self-powered electrochemical lactate biosensor for real-time monitoring of lactic acid. The bioanode and biocathode were modified with D-lactate dehydrogenase (D-LDH) and bilirubin oxidase (BOD), respectively, to facilitate the oxidation and reduction of lactic acid and molecular oxygen. The bioelectrodes were arranged in a parallel configuration to construct the biofuel cell. This biofuel cell’s current–voltage characteristic was analyzed in the presence of various lactic acid concentrations over a range of 1–25 mM. An open circuit voltage of 395.3 mV and a short circuit current density of 418.8 µA/cm² were obtained when operating in 25 mM lactic acid. Additionally, a 10 pF capacitor was integrated via a charge pump circuit to the biofuel cell to realize the self-powered lactate biosensor with a footprint of 1.4 cm × 2 cm. The charge pump enabled the boosting of the biofuel cell voltage in bursts of 1.2–1.8 V via the capacitor. By observing the burst frequency of a 10 pF capacitor, the exact concentration of lactic acid was deduced. As a self-powered lactate sensor, a linear dynamic range of 1–100 mM lactic acid was observed under physiologic conditions (37 °C, pH 7.4) and the sensor exhibited an excellent sensitivity of 125.88 Hz/mM-cm2. This electrochemical lactate biosensor has the potential to be used for the real-time monitoring of lactic acid level in biological fluids. View Full-Text
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Baingane, A.; Slaughter, G. Self-Powered Electrochemical Lactate Biosensing. Energies 2017, 10, 1582.
Baingane A, Slaughter G. Self-Powered Electrochemical Lactate Biosensing. Energies. 2017; 10(10):1582.Chicago/Turabian Style
Baingane, Ankit; Slaughter, Gymama. 2017. "Self-Powered Electrochemical Lactate Biosensing." Energies 10, no. 10: 1582.
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