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Energies 2012, 5(11), 4372-4389; doi:10.3390/en5114372

A Novel Miniature Culture System to Screen CO2-Sequestering Microalgae

School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China
State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, China
New Energy Investment Co. Ltd., Beijing 100016, China
Author to whom correspondence should be addressed.
Received: 1 September 2012 / Revised: 23 October 2012 / Accepted: 23 October 2012 / Published: 1 November 2012
(This article belongs to the Special Issue Biomass and Biofuels 2012)
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In this study, a novel 96-well microplate swivel system (M96SS) was built for high-throughput screening of microalgal strains for CO2 fixation. Cell growth under different CO2 supply conditions (0.2, 0.4, 0.8, and 1.2 g L−1 d−1), residual nitrate, and pH value of Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, and Scenedesmus obliquus SJTU-3 were examined in the M96SS and traditional flask cultures. The dynamic data showed there was a good agreement between the systems. Two critical problems in miniature culture systems (intra-well mixing and evaporation loss) were improved by sealed vertical mixing of the M96SS. A sample screen of six microalgal species (Chlorella sp. SJTU-3, Chlorella pyrenoidosa SJTU-2, Selenastrum capricornutum, Scenedesmus obliquus SJTU-3, Chlamydomonas sajao, Dunaliella primolecta) was carried out in flasks and the M96SS. Chlamydomonas sajao appeared to be a robust performer (highest cell density: 1.437 g L−1) in anaerobic pond water with 0.8, and 1.2 g L−1 d−1 CO2. The reliability and efficiency of the M96SS were verified through a comparison of traditional flask culture, M96SS, Lukavský’s system, and a microplate shaker. View Full-Text
Keywords: microalgae; miniature; screening; CO2 fixation; wastewater microalgae; miniature; screening; CO2 fixation; wastewater

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MDPI and ACS Style

Han, W.; Li, C.; Miao, X.; Yu, G. A Novel Miniature Culture System to Screen CO2-Sequestering Microalgae. Energies 2012, 5, 4372-4389.

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