Isolation and Fatty Acid Profile of Selected Microalgae Strains from the Red Sea for Biofuel Production
Abstract
:1. Introduction
2. Results and Discussion
2.1. Pre-enrichment of Cultures
2.2. Isolation of Strains by FACS
2.3. Identification of Strains by 18S rDNA Sequencing
Strain | Phylum | rDNA Marker |
---|---|---|
Nannochloris sp. SBL1 | Chlorophyta | 18S (PS) |
Picochlorum sp. SBL2 | Chlorophyta | 18S (PS), 5.8S (PS), ITS2, 28S (PS) |
Desmochloris sp. SBL3 | Chlorophyta | 18S (PS), 5.8S (PS), ITS2, 28S (PS) |
Nannochloris sp. SBL4 | Chlorophyta | 18S (PS) |
2.4. Fluorescence Microscopy of Cells Stained with BODIPY 505/515
2.5. Total Lipid Content and Fatty Acid Profile
Fatty acid (%) | Nannochloris sp. SBL1 | Picochlorum sp. SBL2 | Desmochloris sp. SBL3 | Nannochloris sp. SBL4 |
---|---|---|---|---|
C15:0 | n.d. | n.d. | 4.19 ± 0.20 | n.d. |
C16:0 | 32.48 ± 3.24 | 26.16 ± 2.03 | 33.63 ± 1.65 | 28.52 ± 1.18 |
C18:0 | 2.28 ± 0.30 | 3.22 ± 0.60 | 1.94 ± 0.59 | n.d. |
∑ SFA | 34.77 ± 3.55 | 29.37 ± 2.62 | 39.76 ± 2.44 | 28.52 ± 1.18 |
C16:1 | 12.19 ± 1.63 | 12.05 ± 1.26 | n.d. | 17.10 ± 1.15 |
C18:1 | 33.82 ± 2.28 | 21.96 ± 1.39 | 28.17 ± 1.22 | 40.41 ± 4.02 |
∑ MUFA | 46.00 ± 3.91 | 34.01 ± 2.65 | 28.17 ± 1.22 | 57.51 ± 5.16 |
C16:2 | n.d. | 7.18 ± 0.20 | n.d. | n.d. |
C16:3 | 3.80 ± 0.48 | 5.68 ± 0.37 | 1.32 ± 0.07 | 4.46 ± 0.64 |
C18:2 | 15.44 ± 2.12 | 23.76 ± 0.36 | 28.55 ± 2.23 | 9.50 ± 0.59 |
C18:3 | n.d. | n.d. | 2.21 ± 0.08 | n.d. |
∑ PUFA | 19.23 ± 2.60 | 36.62 ± 0.93 | 32.07 ± 2.38 | 13.97 ± 1.23 |
Iodine value | 86 | 99 | 84 | 88 |
3. Experimental Section
3.1. Sampling and Storage
3.2. Isolation of Microalgae Strains by Fluorescence Activated Cell Sorting
3.3. Identification of Strains by rDNA Sequencing
Gene locus | Primer name | Sequence (5’ to 3’) |
---|---|---|
18S | 18SUnivFor | ACCTGGTTGATCCTGCCAGT |
18S | 18SUnivRev | TCAGCCTTGCGACCATAC |
5.8S, ITS2, 28S | 5.8SFor | AAGAACGCAGCGAAATGC |
5.8S, ITS2, 28S | 28SRev | GACTCCTTGGTCCGTGTTTC |
3.4. Microscopy
3.5. Assessment of Total Lipid Content and Fatty Acid Profile by Gas Chromatography Coupled with Mass Spectrometry
4. Conclusions
Acknowledgments
Conflict of Interest
References
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Pereira, H.; Barreira, L.; Custódio, L.; Alrokayan, S.; Mouffouk, F.; Varela, J.; Abu-Salah, K.M.; Ben-Hamadou, R. Isolation and Fatty Acid Profile of Selected Microalgae Strains from the Red Sea for Biofuel Production. Energies 2013, 6, 2773-2783. https://doi.org/10.3390/en6062773
Pereira H, Barreira L, Custódio L, Alrokayan S, Mouffouk F, Varela J, Abu-Salah KM, Ben-Hamadou R. Isolation and Fatty Acid Profile of Selected Microalgae Strains from the Red Sea for Biofuel Production. Energies. 2013; 6(6):2773-2783. https://doi.org/10.3390/en6062773
Chicago/Turabian StylePereira, Hugo, Luísa Barreira, Luísa Custódio, Salman Alrokayan, Fouzi Mouffouk, João Varela, Khalid M. Abu-Salah, and Radhouan Ben-Hamadou. 2013. "Isolation and Fatty Acid Profile of Selected Microalgae Strains from the Red Sea for Biofuel Production" Energies 6, no. 6: 2773-2783. https://doi.org/10.3390/en6062773