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Viruses 2013, 5(11), 2840-2855; doi:10.3390/v5112840

HPV Genotyping 9G Membrane Test

1 Institute for Applied Chemistry and Department of Chemistry, Hallym University, Chuncheon, 200-702, Korea 2 Biometrix Technology, Inc. 202 BioVenture Plaza, Chuncheon, 200-161, Korea 3 Department of Pathology, College of Medicine, CHA University, 487-801, Seongnam, Korea
* Author to whom correspondence should be addressed.
Received: 21 October 2013 / Revised: 6 November 2013 / Accepted: 19 November 2013 / Published: 22 November 2013
(This article belongs to the Section Animal Viruses)
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The results of the genital human papillomavirus (HPV) detection in 439 cervical samples by cervical cytology were compared with sequencing analysis and a newly developed HPV genotyping 9G membrane test. The excellent sensitivity and specificity of the HPV genotyping 9G membrane test was assured by a signal to noise ratio of more than 300 and a target hybridization to non-target hybridization ratio of 300 ~ 400 at 25 °C. The final results can be obtained in 29 min by simple loading of the hybridization and washing solutions and scanning the membranes without any drying steps or special handling. The 100% identical results of the HPV genotyping 9G membrane test with sequencing results in 439 clinical samples demonstrate significant clinical application for this test. HPV genotyping 9G membrane tests can identify and discriminate five HR-HPV genotypes which are prevalent in almost 87% of cervical cancer cases. Its simple handling makes the HPV genotyping 9G membrane test a very convenient platform for accurate HPV genotyping.
Keywords: HPV; cervical neoplasia; genotyping; 9G membrane; cervical cancer HPV; cervical neoplasia; genotyping; 9G membrane; cervical cancer
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Sayyed, D.R.; Song, K.-S.; Nimse, S.B.; An, H.; Kim, J.; Kim, T. HPV Genotyping 9G Membrane Test. Viruses 2013, 5, 2840-2855.

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