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Viruses 2017, 9(8), 220; doi:10.3390/v9080220

The Potential for Reassortment between Oropouche and Schmallenberg Orthobunyaviruses

1
MRC-University of Glasgow Centre for Virus Research, 464 Bearsden Road, Glasgow G61 1QH, UK
2
Biomedical Sciences Research Complex, School of Biology, University of St Andrews, St Andrews KY16 9ST, UK
3
Department of Microbiology, National Emerging Infectious Disease Laboratories, Boston University School of Medicine, Boston, MA 02118, USA
4
Universidade Federal da Fronteira Sul, Campus Passo Fundo. Rodovia RS 153 - Km 03, Jardim América, CEP 99034-600 Passo Fundo—RS, Brasil
In Memoriam.
*
Authors to whom correspondence should be addressed.
Academic Editor: Andrew Mehle
Received: 1 July 2017 / Revised: 3 August 2017 / Accepted: 6 August 2017 / Published: 11 August 2017
(This article belongs to the Section Animal Viruses)
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Abstract

A number of viruses within the Peribunyaviridae family are naturally occurring reassortants, a common phenomenon for segmented viruses. Using a minigenome-reporter and virus-like particle (VLP) production assay, we have accessed the potential of Oropouche virus (OROV), Schmallenberg virus (SBV), and other orthobunyaviruses within the Simbu serogroup to reassort. We found that the untranslated region (UTR) in the medium segment is a potential contributing factor for reassortment by the tested viruses. We demonstrate that for promoter activity to occur it was essential that the viral RNA polymerase (L) and nucleocapsid (N) proteins were from the same virus, reinforcing the hypothesis that the large and small segments that encode these proteins segregate together during genome reassortment. Our results indicate that, given the right epidemiological setting, reassortment between SBV and OROV would potentially be feasible and could contribute to the emergence of a new Simbu virus. View Full-Text
Keywords: Oropouche virus; Schmallenberg virus; bunyavirus; Orthobunyavirus; Simbu serogroup; minigenome; reassortment Oropouche virus; Schmallenberg virus; bunyavirus; Orthobunyavirus; Simbu serogroup; minigenome; reassortment
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MDPI and ACS Style

Tilston-Lunel, N.L.; Shi, X.; Elliott, R.M.; Acrani, G.O. The Potential for Reassortment between Oropouche and Schmallenberg Orthobunyaviruses. Viruses 2017, 9, 220.

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