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Toxins 2017, 9(5), 167; doi:10.3390/toxins9050167

iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A

1
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China
2
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China
3
College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China
4
Beijing Laboratory for Food Quality and Safety, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
5
School of Public Health, Zunyi Medical University, Zunyi 563000, China
*
Author to whom correspondence should be addressed.
Academic Editors: Richard A. Manderville and Annie Pfohl-Leszkowicz
Received: 5 April 2017 / Revised: 9 May 2017 / Accepted: 15 May 2017 / Published: 18 May 2017
(This article belongs to the Collection Ochratoxins-Collection)
View Full-Text   |   Download PDF [1996 KB, uploaded 22 May 2017]   |  

Abstract

Ochratoxin A (OTA) is one of the most common and dangerous mycotoxins in the world. Previous work indicated that OTA could elicit spontaneous HR-like lesions formation Arabidopsis thaliana, reactive oxygen species (ROS) play an important role in OTA toxicity, and their major endogenous source is mitochondria. However, there has been no evidence as to whether OTA induces directly PCD in plants until now. In this study, the presence of OTA in Arabidopsis thaliana leaves triggered accelerated respiration, increased production of mitochondrial ROS, the opening of ROS-dependent mitochondrial permeability transition pores and a decrease in mitochondrial membrane potential as well as the release of cytochrome c into the cytosol. There were 42 and 43 significantly differentially expressed proteins identified in response to exposure to OTA for 8 and 24 h, respectively, according to iTRAQ analysis. These proteins were mainly involved in perturbation of the mitochondrial electron transport chain, interfering with ATP synthesis and inducing PCD. Digital gene expression data at transcriptional level was consistent with the cell death induced by OTA being PCD. These results indicated that mitochondrial dysfunction was a prerequisite for OTA-induced PCD and the initiation and execution of PCD via a mitochondrial-mediated pathway. View Full-Text
Keywords: ochratoxin A; Arabidopsis thaliana; programmed cell death; mitochondria; iTRAQ; mitoproteomes ochratoxin A; Arabidopsis thaliana; programmed cell death; mitochondria; iTRAQ; mitoproteomes
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Wang, Y.; Peng, X.; Yang, Z.; Zhao, W.; Xu, W.; Hao, J.; Wu, W.; Shen, X.L.; Luo, Y.; Huang, K. iTRAQ Mitoproteome Analysis Reveals Mechanisms of Programmed Cell Death in Arabidopsis thaliana Induced by Ochratoxin A. Toxins 2017, 9, 167.

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