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Toxins 2017, 9(5), 172; doi:10.3390/toxins9050172

Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin

1,2,3,4,†
,
1,5,†
,
6,†
,
6
,
6
,
6
,
1,2,3,4
,
1,2,3,4
,
1,2,3,4
and
1,2,3,4,*
1
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
2
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
3
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China
4
Laboratory of Risk Assessment for Oilseeds Products (Wuhan), Ministry of Agriculture, Wuhan 430062, China
5
College of Food Science and Technology, South China Agricultural University, Guangzhou 510000, China
6
Hubei Provincial Institute for Food Supervision, No. 666, Gaoxin Road, F District, Wuhan East Lake High-Tech Development Zone, Wuhan 430000, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Laura Anfossi
Received: 28 February 2017 / Revised: 3 May 2017 / Accepted: 17 May 2017 / Published: 19 May 2017
(This article belongs to the Collection Aflatoxins)
View Full-Text   |   Download PDF [975 KB, uploaded 22 May 2017]   |  

Abstract

Research about antibody specificity spectra was conducted to develop single-specific antibodies or broad-specific antibodies. Aflatoxins, as one class of high-toxicity mycotoxins, were selected as the research targets to investigate the effect of the immunogen dose on antibody specificity spectra. For this aim, 16 monoclonal antibodies were induced by low or high doses of aflatoxin B1-BSA, and 34 monoclonal antibodies were induced by low or high doses of aflatoxin M1-BSA. The specificities of the antibodies induced, whether by aflatoxin B1 conjugate or aflatoxin M1 conjugate, indicated that the low dose of the immunogen induced a narrow spectrum of antibody specificity, while the high dose of the immunogen showed an advantage to form a broad spectrum of antibody specificity. Therefore, this report provides important information for the development of new antibodies against small molecules like aflatoxins. View Full-Text
Keywords: specificity spectrums; dose of immuogen; antibodies; aflatoxin specificity spectrums; dose of immuogen; antibodies; aflatoxin
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Li, P.; Wu, J.; Zhang, L.; Fan, Z.; Yu, T.; Jiang, F.; Tang, X.; Zhang, Z.; Zhang, W.; Zhang, Q. Doses of Immunogen Contribute to Specificity Spectrums of Antibodies against Aflatoxin. Toxins 2017, 9, 172.

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