Next Article in Journal
Resistless Fabrication of Nanoimprint Lithography (NIL) Stamps Using Nano-Stencil Lithography
Next Article in Special Issue
Hydrodynamic Cell Trapping for High Throughput Single-Cell Applications
Previous Article in Journal / Special Issue
Recent Trends on Micro/Nanofluidic Single Cell Electroporation
Article Menu

Export Article

Open AccessArticle
Micromachines 2013, 4(4), 357-369; doi:10.3390/mi4040357

Polydimethylsiloxane (PDMS) Sub-Micron Traps for Single-Cell Analysis of Bacteria

Institute of Bio- and Geosciences, IBG-1: Biotechnology, Forschungszentrum Jülich GmbH, D-52428 Jülich, Germany
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 25 July 2013 / Revised: 12 September 2013 / Accepted: 23 September 2013 / Published: 11 October 2013
(This article belongs to the Special Issue Micro/Nanofluidic Devices for Single Cell Analysis)
View Full-Text   |   Download PDF [3816 KB, uploaded 11 October 2013]   |  

Abstract

Microfluidics has become an essential tool in single-cell analysis assays for gaining more accurate insights into cell behavior. Various microfluidics methods have been introduced facilitating single-cell analysis of a broad range of cell types. However, the study of prokaryotic cells such as Escherichia coli and others still faces the challenge of achieving proper single-cell immobilization simply due to their small size and often fast growth rates. Recently, new approaches were presented to investigate bacteria growing in monolayers and single-cell tracks under environmental control. This allows for high-resolution time-lapse observation of cell proliferation, cell morphology and fluorescence-coupled bioreporters. Inside microcolonies, interactions between nearby cells are likely and may cause interference during perturbation studies. In this paper, we present a microfluidic device containing hundred sub-micron sized trapping barrier structures for single E. coli cells. Descendant cells are rapidly washed away as well as components secreted by growing cells. Experiments show excellent growth rates, indicating high cell viability. Analyses of elongation and growth rates as well as morphology were successfully performed. This device will find application in prokaryotic single-cell studies under constant environment where by-product interference is undesired.
Keywords: microfluidics; single-cell analysis; E. coli; bacteria microfluidics; single-cell analysis; E. coli; bacteria
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Probst, C.; Grünberger, A.; Wiechert, W.; Kohlheyer, D. Polydimethylsiloxane (PDMS) Sub-Micron Traps for Single-Cell Analysis of Bacteria. Micromachines 2013, 4, 357-369.

Show more citation formats Show less citations formats

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Micromachines EISSN 2072-666X Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top