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Polymers 2017, 9(4), 144; doi:10.3390/polym9040144

Morphology Control of Ni(II)-NTA-End-Functionalized Block Copolymer and Bio-Conjugation through Metal-Ligand Complex

1
Department of Polymer Science and Engineering, Pusan National University, Busan 46241, Korea
2
Department of Chemistry, Sylhet Government Women’s College, Sylhet 3100, Bangladesh
3
Research Center for Bio-Based Chemistry, Korea Research Institute of Chemical Technology (KRICT), Daejeon 34114, Korea
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 6 February 2017 / Revised: 11 April 2017 / Accepted: 17 April 2017 / Published: 20 April 2017
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Abstract

This study demonstrates the synthesis of an amphiphilic block copolymer, Ni2+-nitrilotiracetic acid-end-functionalized-poly(poly(ethylene glycol)methyl ether methacrylate)-block-polystyrene (NTA-p(PEGMA-b-St)), morphology control via their self-assembly behavior and reversible bioconjugation of hexahistidine-tagged green fluorescent protein (His6-GFP) onto the surfaces of polymeric vesicles through nitrilotriacetic acid (NTA)-Ni2+-His interaction. First, the t-boc-protected-NTA-p(PEGMA-b-St) was synthesized by atom transfer radical polymerization. After the removal of the t-boc protecting group, the NTA group of the polymer was complexed with Ni2+. To induce self-assembly, water was added as a selective solvent to the solution of the copolymer in tetrahydrofuran (THF). Varying the water content of the solution resulted in various morphologies including spheres, lamellas and vesicles. Finally, polymeric vesicles decorated with green fluorescent protein (GFP) on their surfaces were prepared by the addition of His6-GFP into the vesicles solution. Reversibility of the binding between vesicles and His6-GFP was confirmed with a fluorescent microscope. View Full-Text
Keywords: atom transfer radical polymerization (ATRP); end-functionalized block copolymer; nitrilotriacetic acid (NTA); self-assembly behavior; protein–polymer hybrid atom transfer radical polymerization (ATRP); end-functionalized block copolymer; nitrilotriacetic acid (NTA); self-assembly behavior; protein–polymer hybrid
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Park, D.; Lee, C.; Chae, M.; Kadir, M.A.; Choi, J.E.; Song, J.K.; Paik, H.-J. Morphology Control of Ni(II)-NTA-End-Functionalized Block Copolymer and Bio-Conjugation through Metal-Ligand Complex. Polymers 2017, 9, 144.

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