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Genes 2017, 8(8), 193; doi:10.3390/genes8080193

Characterization and Expression Profiling of Camellia sinensis Cinnamate 4-hydroxylase Genes in Phenylpropanoid Pathways

1,2,†
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1,2,†
,
2
,
1,2
,
1,2
,
1,2
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1,2,* and 2,*
1
School of Life Science, Anhui Agricultural University, Hefei 230036, Anhui, China
2
State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 West Changjiang Rd, Hefei 230036, Anhui, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 27 June 2017 / Revised: 23 July 2017 / Accepted: 25 July 2017 / Published: 1 August 2017
(This article belongs to the Section Plant Genetics and Genomics)
View Full-Text   |   Download PDF [5594 KB, uploaded 1 August 2017]   |  

Abstract

Cinnamate 4-hydroxylase (C4H), a cytochrome P450-dependent monooxygenase, participates in the synthesis of numerous polyphenoid compounds, such as flavonoids and lignins. However, the C4H gene number and function in tea plants are not clear. We screened all available transcriptome and genome databases of tea plants and three C4H genes were identified and named CsC4Ha, CsC4Hb, and CsC4Hc, respectively. Both CsC4Ha and CsC4Hb have 1518-bp open reading frames that encode 505-amino acid proteins. CsC4Hc has a 1635-bp open reading frame that encodes a 544-amino acid protein. Enzymatic analysis of recombinant proteins expressed in yeast showed that the three enzymes catalyzed the formation of p-coumaric acid (4-hydroxy trans-cinnamic acid) from trans-cinnamic acid. Quantitative real-time PCR (qRT-PCR) analysis showed that CsC4Ha was highly expressed in the 4th leaf, CsC4Hb was highly expressed in tender leaves, while CsC4Hc was highly expressed in the young stems. The three CsC4Hs were induced with varying degrees by abiotic stress treatments. These results suggest they may have different subcellular localization and different physiological functions. View Full-Text
Keywords: Camellia sinensis; phenylpropanoid; cinnamate 4-hydroxylase; expression pattern; abiotic stress Camellia sinensis; phenylpropanoid; cinnamate 4-hydroxylase; expression pattern; abiotic stress
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Xia, J.; Liu, Y.; Yao, S.; Li, M.; Zhu, M.; Huang, K.; Gao, L.; Xia, T. Characterization and Expression Profiling of Camellia sinensis Cinnamate 4-hydroxylase Genes in Phenylpropanoid Pathways. Genes 2017, 8, 193.

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