Next Article in Journal
Mutation Clusters from Cancer Exome
Previous Article in Journal
Two C3H Type Zinc Finger Protein Genes, CpCZF1 and CpCZF2, from Chimonanthus praecox Affect Stamen Development in Arabidopsis
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessArticle
Genes 2017, 8(8), 200; doi:10.3390/genes8080200

Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis

College of Biological Sciences and Technology, Beijing Forestry University, Beijing 100083, China
*
Author to whom correspondence should be addressed.
Received: 7 July 2017 / Revised: 4 August 2017 / Accepted: 7 August 2017 / Published: 11 August 2017
(This article belongs to the Section Plant Genetics and Genomics)
View Full-Text   |   Download PDF [1818 KB, uploaded 14 August 2017]   |  

Abstract

Most of the eukaryotic genes contain introns, which are removed from the pre-RNA during RNA processing. In contrast to the introns in animals, which are usually several kilo base pairs (kb), those in plants generally are very small, which are mostly from dozens of base pairs (bp) to a few hundred bp. According to annotation version 10.0 of the genome of Arabidopsis thaliana, there are 127,854 introns in the nuclear genes; 99.23% of them are less than 1 kb, and only 16 introns are annotated to be larger than 5 kb, which are extremely large introns (ELI) in Arabidopsis. To learn whether these introns are true introns or not and how large introns could be in Arabidopsis, RT-PCR analysis of genes containing these ELIs were carried out. The results indicated that some of these putative introns are indeed ELIs. These ELIs are mainly composed of transposons or transposable elements (TE), excepting one, whose counterparts are also very long in diverse plant species. Thus, this study confirms the existence of introns larger than 5 kb or even 10 kb in Arabidopsis. View Full-Text
Keywords: Arabidopsis; large intron; transposon; reverse transcription PCR Arabidopsis; large intron; transposon; reverse transcription PCR
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Chang, N.; Sun, Q.; Hu, J.; An, C.; Gao, H. Large Introns of 5 to 10 Kilo Base Pairs Can Be Spliced out in Arabidopsis. Genes 2017, 8, 200.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Genes EISSN 2073-4425 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top