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Article

Optimization of Bioelectricity Generation in Constructed Wetland-Coupled Microbial Fuel Cell Systems

1
School of Energy and Environment, Southeast University, Nanjing 210096, China
2
School of Civil Engineering, Southeast University, Nanjing 210096, China
*
Author to whom correspondence should be addressed.
Water 2017, 9(3), 185; https://doi.org/10.3390/w9030185
Submission received: 27 November 2016 / Revised: 24 February 2017 / Accepted: 3 March 2017 / Published: 6 March 2017

Abstract

:
Constructed wetland-coupled microbial fuel cell systems (CW-MFCs) incorporate an aerobic zone and an anaerobic zone to generate electricity that achieves the oxidative degradation of contaminants. However, there are few reports on the performance of such coupled systems. In this study, we determined the optimal configuration of CW-MFCs to characterize their electricity generation performance. Based on the results using different levels of dissolved oxygen among the CW-MFCs, we concluded that a 20-cm distance between the anode and cathode produced an optimal removal of chemical oxygen demand (COD) of 94.90% with a 0.15 W/m3 power density, 339.80 Ω internal resistance, and 0.31% coulombic efficiency. In addition, a COD of 200 mg/L provided greater electricity generation (741 mV open circuit voltage, 0.20 W/m3 power density, 339.80 Ω internal resistance, and 0.49 mA current) and purification ability (90.45% COD removal) to meet system COD loading limitations than did higher COD values. By adding 50 mM phosphate buffer solution to synthetic wastewater, relatively high conductivity and buffer capacity were achieved, resulting in improvement in electricity generation. These findings highlight important aspects of bioelectricity generation in CW-MFCs.

1. Introduction

Microbial fuel cells (MFCs), which make use of domestic sewage, industrial effluent, leachate, sediment, and rhizodeposits as biodegradable substrates, offer a technology for electricity generation in addition to benefits for the environment [1,2,3]. Organic matter can be used as a renewable resource to generate electrons and protons via electrochemically active bacteria in MFCs [4,5,6]. Protons are released into solution and electrons are produced at the anode; subsequently, at the interface of the cathode, electrons pass through the outer circuit before they can reach the cathode and combine with electron acceptors [7,8]. Single-chamber MFCs with an anion exchange membrane (AEM), which serves to thoroughly separate the aerobic and anaerobic regions, are the most widely studied MFC systems [9,10].
Numerous studies have focused on exploiting constructions in MFCs [11], and there is a need to study the systems with electric potential between the anode and cathode. Various MFC systems with such configurations have been the focus of recent studies. Plant-MFCs and MFC-coupled constructed wetlands (CW-MFCs) have also been studied [12,13]. In one such system, microbes in the anaerobic anode compartment, where the roots of plants were located, were able to use the carbon dioxide fixed by photosynthesis to convert rhizodeposits (e.g., sugars, organic acids, polymeric carbohydrates, and enzymes), and achieved a maximum power density of 67 mW/m2 [14]. In fact, roots can release a lot of oxygen, and the oxygen promoted the electricity generation performance of the cathode. Chiranjeevi et al. integrated an MFC with a submerged and emergent macrophyte-based system to harvest energy [15]. The roots do not provide organic carbon to the anode.
The design of the vertical subsurface-flow CW-MFC (VSFCW-MFC) is not drawn from the empirical design of conventional CWs because it needs to be suitable for electricity generation. Taking high power density as an example, the system requires low internal resistance, which is achieved by reducing the distance between the two electrodes and increasing the conductivity of the electrolyte. Previous studies have suggested that a suitable distance between the anode and cathode is 18−62 cm. Phosphate buffer solution (PBS) in the MFC contributes to maintaining a neutral pH for microorganisms, increasing conductivity, and avoiding the transportation limitation of protons in the biofilm by forming the conjugate acid of the buffer [16]. A concentrated PBS is expected to improve the performance of the system. Moreover, the rate of biodegradation is limited, in particular for some complex polymeric organic substrates [17]. Polymeric organic substrate wastewater passing through the anode flushes the cathode and causes a decrease in the cathode potential, which results in a decrease in electricity production. Villasenor et al. reported that degradation efficiency decreased with increasing chemical oxygen demand (COD) and concentration, and correspondingly, the electricity produced in a horizontal subsurface flow CW-MFC decreased [18].
Most studies have focused on the effects of redox conditions [19], electrode material [20], plants and bacteria [21], and flow models of organic loading [19]. However, few studies have explored the effects of the system structure and presence of PBS on performance. Therefore, we investigated the effects of varying the distance between the electrodes, influent COD loading, and PBS to improve the electricity generation and pollutant removal of a VSFCW-MFC.

2. Materials and Methods

2.1. Construction of the Fuel Cell

The MFC was integrated with a vertical organic glass pipe (internal diameter, 30 cm; height, 50 cm) with sampling outlets every 5 cm along its length and was filled with sections consisting of a gravel layer (gravel diameter, 3 mm, Nanjing Zhongdong Chemical Glass Instrument Co., Ltd, Nanjing, China), the anodic electrode layer (thickness, 10 cm), a second gravel layer, and the cathode layer (thickness, 2.5 cm), as shown in Figure 1a. Both electrodes consisted of granular activated carbon (GAC; 3–5 mm in diameter and 500–900 m2/g in specific area, Jiangsu Zhuxi Activated Carbon Co., Ltd., Nanjing, China) pretreated according to previous methods [22]. A stainless steel mesh current collector (304SS; 30-cm total diameter, 3-mm thickness, 1.7-mm mesh size, and 0.42-mm wire diameter, Nanjing Zhongdong Chemical Glass Instrument Co., Ltd., Nanjing, China) buried in the electrode was linked to the external circuit with an insulated titanium wire; the circuit was connected to a data acquisition module to measure the voltage (V) data (DAM-3058R and DAM-3210, Art Technology Co. Ltd., Beijing, China). The total volume of the system was 35.3 L, with a total effective working volume of 12.4 L, compared to an effective volume of 2.1 L for the anode. After they had been planted for 1 month at an ambient temperature of 28 °C, 10 Phragmites australis were transplanted in the cathode layer. To culture the microorganisms, anaerobic sludge (mixed liquor suspended solids, 60 g/L) was collected from the East City Municipal Wastewater Treatment Plant, Nanjing, China. Condensed anaerobic slurry (GAC: Sludge volumetric ratio, 2.5:1) was added to the VSFCW-MFC, and synthetic wastewater (SW) was transported using a peristaltic pump (BT100-1L, Baoding Longer Precision Pump Co., Ltd., Baoding, China). The nutrient solution used in the start-up phase contained: Glucose, 200.00 mg/L; KNO3, 252.50 mg/L; K2HPO4·3H2O, 26.32 mg/L; NaHCO3, 336.00 mg/L; NaCl 330.00, mg/L; and 10.00 mL micronutrients [1]. Based on our pre-experiment, the hydraulic retention time was estimated to be 48 h, and the flow rate of the system was 6.36 mL/min (surface loading rate: 0.0055 m3/(m2·h)). The VSFCW-MFCs were covered with black adhesive tape to prevent algae growth. All experiments were operated continuously for 3 months indoors at 28 ± 2 °C with a relative humidity of 55%–70%. The nutrient solution (first trial: SW with PBS, Table 1) was used during the stable period.

2.2. Electrochemical and Chemical Monitoring

A variation of output voltage of approximately 10% per day indicated stabilization in the CW-MFCs. The voltage between the anode and cathode was recorded automatically every 5 min using a data acquisition module (DAM-3058R and DAM-3210, Art Technology Co. Ltd., Beijing, China). The current density was calculated following Ohm’s Law based on the effective working volume (2.1 L) of the anode and the power density equation given below:
P = I·U/V
where P is power density, I is current, U is the voltage, and V the effective working volume (2.1 L) of the anode.
Pt = I·U/V
where Pt is power density, I is current, U is the voltage, and V the total effective working volume (12.4 L) of the VSFCW-MFC.
The polarization profile [23] was obtained 1 week after a period of stable operation (after 20 days of operation), during which the resistance load increased from 5 to 105 Ω. The open circuit voltage was not measured until the system had been kept open for 12 h.
The coulombic efficiency resulting from the redox reaction was calculated from the following equation [1]:
η C E = I F × 4 32 × Q i n × Δ C O D
where ηCE is coulombic efficiency, F represents the Faraday constant, Qin represents the water flow of influent (L/s), and ΔCOD is the variation in effluent and influent COD.
The dissolved oxygen (DO) of the wastewater from the sampling outlets was measured using a DO instrument (DO-31P; DKK-TOA, Tokyo, Japan) during the period of stable operation. The standard potential of calomel reference electrode was made by the Shanghai INESA Scientific Instrument Co., Ltd. (323-01; Shanghai, China). COD was monitored according to the American Public Health method [24]. Linear regression was used to analyze the correlation between voltage and DO concentration with Microsoft Excel 2010.

2.3. Operating Parameters

To determine the effects of changing the distance between the two electrodes, the anode was positioned 10, 20, 30, and 40 cm from the cathode in the period of stable operation (Figure 1b). The components of the SW with and without PBS are shown in Table 1 [1,25,26].

3. Results and Discussion

3.1. Effects of DO on Voltage in the VSFCW-MFC System

The average voltages of the different distances between the two electrodes, except for the start-up stage, are shown in Figure 2a. The 20-cm spacing had a voltage of around 560 mV throughout the experiment, while the 30-cm spacing yielded a voltage of 520 mV. However, the 10-cm and 40-cm spacings produced voltages of only about 440 mV and 420 mV, respectively.
Organic matter in wastewater is degraded and consumed by DO, and thus forms anoxic conditions suitable for catalytic exoelectrogens. Oxygen demand in the wastewater decreased and DO was gradually replenished, entering the cathode compartment at a high concentration compared to other studies [18]. In addition, the macrophytes raised the DO concentration and simultaneously produced negligible rhizodeposits.
The variation in DO was measured at the sampling outlets along the VSFCW-MFCs (Figure 2b). When the same SW was fed into the system and electron accepters were neglected, the cathode potential was determined primarily by the DO concentration in the MFCs [27]. The anaerobic condition was related to the concentration of the exoelectrogens acting as efficient catalysts, which suggests that electricity production was closely related to the DO level. First, DO decreased to a minimum, and then increased starting about halfway along the length of the system. Comparing the four systems, the minimum DO (0.43 mg/L) occurred in the 20-cm electrode spacing, and the maximum (4.73 mg/L) was unexpectedly observed in the cathode. The electrode spacings of 10, 30, and 40 cm yielded minimum DO concentrations of 0.60, 0.54, and 0.52 mg/L, respectively, and maximum DO concentrations of 4.30, 4.47, and 3.75 mg/L, respectively. In addition, the DO in the anode zones was 0.60−1.23 mg/L, 0.77−1.135 mg/L, and 0.79−1.37 mg/L, respectively. The variation in DO between the anode and cathode of the systems with different electrode spacings was significantly (Linear regression, r = −0.991, p < 0.001) negatively correlated with the difference in voltage (Figure 2c).

3.2. Bioelectricity Generation with Different Electrode Spacings

The polarization and power density profiles with different external resistances are shown in Figure 3. The maximum open circuit voltage of 741 mV was achieved for the 20-cm spacing with a maximum current density of 0.49 A/m3 and maximum power density of 0.20 W/m3 (Pt = 0.034 W/m3) with an external resistance of approximately 400 Ω. An open circuit voltage of 717 mV was measured in the 30-cm spacing system, which obtained a maximum current density of 0.45 A/m3 and maximum power density of 0.17 W/m3 (Pt = 0.029 W/m3) with an external resistance of approximately 400 Ω. Lower performances were observed when the electrode spacing was 10 cm and 40 cm, with a maximum open circuit voltage of 671 and 635 mV, respectively, maximum current density of 0.37 A/m3 and 0.36 A/m3, and maximum power density of 0.12 W/m3 (Pt = 0.020 W/m3)and 0.11 W/m3 (Pt = 0.019 W/m3). The internal resistance (Rint) was 385.80, 339.80, 367.68, and 406.32 Ω for a spacing of 10, 20, 30, and 40 cm, respectively, using the linear part of the polarization curve (the external resistance varied between 100 and 1000 Ω).
Without distinguishing the resistance from different sources, the internal resistance consisted of the activation resistance in the electrodes, the ohmic resistance, and the diffusion resistance. Fan et al. proposed an equation to quantify the distribution of the internal resistance, and showed that the distance between the anode and cathode was inversely proportional to the power generation [28]. However, except for the 10-cm spacing system (DO present in the system also affected the resistance distribution), the resistance decreased when the spacing of the two electrodes decreased from 40 to 20 cm. The maximum current density and minimum internal resistance were achieved simultaneously with an electrode spacing of 20 cm. However, the resistance of the VSFCW-MFC was high, consistent with a previous report (internal resistance 188 Ω) [15]. This might be attributable to the poor contact between the GAC and the steel mesh as well as the large distance between the two electrodes, which caused a decrease in the power density compared to the 9.8 W/m3 with SW [18,29].
The variation in COD with different electrode spacing versus reactor height is shown in Figure 4, and the performance of the VSFCW-MFC energy output is summarized in Table 2. The reactors were operated with consecutive flow and an external resistance of 1000 Ω.
The COD removal efficiency decreased with increasing electrode spacing in the VSFCW-MFCs (Table 2). In addition, the electrical current does not enhance COD removal but the COD removal results in an electrical current [30]. However, varying the position of the anodic compartment did not affect DO.
In this study, the 20-cm electrode spacing achieved the maximum COD removal efficiency of 94.90%, which resulted in a higher energy output with a power density of 0.0045 W/m2 (Pt = 0.025 W/m3) and fairly low coulombic efficiency of 0.31%. This might be because acetoclastic methanogens compete with exoelectrogens for the same electron donor (acetate) and consume excess biodegradable carbon [31,32,33]. In addition, electron acceptors such as nitrate, sulfate, and oxygen may have caused the low efficiency.

3.3. Effect of Influent COD on Bioelectricity Generation

The COD concentration in influent affects the performance of the VSFCW-MFC reactor. The COD concentration should satisfy the microorganisms’ requirements so that sufficient numbers of electrons are released for electricity production in the system.
The COD removal with the 20-cm electrode spacing at different influent COD concentrations is shown in Figure 5. With concentrations of 800, 400, and 200 mg/L, the average COD removal efficiencies reached 90.76%, 90.99%, and 90.45%, respectively.
The maximum power densities and coulombic efficiencies for different influent COD concentrations are illustrated in Table 3. When the concentration increased from 200 to 800 mg/L, negative open circuit potentials of the anode varying from −500 to −526 mV (vs. SCE) were observed. These results suggest that a higher COD concentration was beneficial for enhancing the active catalysis of exoelectrogens. This was because microorganisms consumed more oxygen to degrade the increased organic matter or SW with complex compounds with negative potential, and thus caused the open circuit potential to decrease. The anode potential varied from −0.25 to 0.21 V, which increased the maximum current, suggesting that an anode potential in the range of −526 to −500 mV might enhance current generation [34]. The cathode open circuit potential simultaneously decreased from 241 to 101 mV (vs. SCE). This marked decrease resulted in a decrease in the open circuit voltage from 741 to 627 mV. Previous studies have reported no improvement in COD removal efficiency by increasing the COD concentration. However, the organic matter was consumed when the cathode was exposed to diverse Methanobacterium [35]. The oxidation-induced consumption of overflow organic matter from the upper cathode compartment decreased the cathode potential. While the anode potential also decreased, the cathode controlled the performance of the VSFCW-MFC [18]. Power generation was limited by the cathode, although the exoelectrogens also significantly affected the performance of the system [28]. In addition, the coulombic efficiency and the maximum power density indicated that electron transfer to the external circuit was affected by overabundant COD, and that increases in the COD concentration did not increase the power density of the system, while oxygen was utilized as an electron accepter for the degradation of overflow SW by bacteria, rather than reacting with the electrons from the cathode. Based on the polarization curve, the internal resistance varied with different influent COD concentrations (Ro, 200 = 339.80 Ω; Ro, 400 = 360.50 Ω; and Ro, 800 = 395.55 Ω). Despite the variation in electrolyte resistance, our results suggest that the accumulation of microorganisms feeding on higher COD concentrations can block electron conduction on the interface of the activated carbon acting as an electrode, which results in higher internal resistance. Therefore, COD is a key parameter in the VSFCW-MFC, and it is critical to control its optimal range in the influent to optimize power generation and the treatment of wastewater.

3.4. Electrical Performance with the Addition of PBS

A VSFCW-MFC with an external resistor of 1000 Ω was assembled to investigate the electrical performance with the addition of PBS. Based on the previous experiment, the electrode spacing and the influent COD concentration were controlled at 20 cm and 200 mg/L, respectively. The experiment was separated into three periods. The systems were operated at stable conditions for about three weeks for each period.
As shown in Figure 6a, the system reached a stable current density of 0.261 A/m3 with the addition of 50 mM PBS. After dosing with PBS was stopped, the current density was maintained at about 0.187 A/m3. Subsequently, the system was switched to 330 mg/L with the addition of sodium chloride, and the stable current density was approximately 0.204 A/m3. Electrical conductivity was measured at 8.25, 1.24, and 2.38 mS/cm, in Periods 1, 2, and 3, respectively. The conductivity of adding 50 mM PBS was almost seven-fold that of the system with no PBS. Due to the increased conductivity accelerating the transfer of protons in solution, the system current density increased accompanied by increased conductivity of the SW.
The polarization curves and the power densities for the different periods are shown in Figure 6b. The open circuit voltage reached 856, 706, and 741 mV for Periods 1, 2, and 3, respectively. The maximum power density was 0.37 (Pt = 0.063), 0.17 (Pt = 0.029), and 0.20 W/m3 (Pt = 0.034 W/m3), and the current density was approximately 0.94, 0.45, and 0.49 A/m3, respectively. As already mentioned, the internal resistances were 209.78, 397.23, and 339.80 Ω during the three periods. The wastewater had the maximum conductivity and minimum internal resistance when PBS was added. Hence, electricity generation was enhanced by increasing the conductivity and buffer capability.
Ohmic resistance is related to wastewater conductivity and electrode conductivity [17]. Zhu et al. showed that typical domestic wastewater and industrial effluent have a conductivity of 1 mS/cm, while the electric conductivity was lower in a vertical subsurface flow constructed wetland [7]. Because the internal ohmic losses of our system were high, increasing the solution conductivity to reduce the internal resistance of the artificial wetlands was beneficial. Compared to the original wastewater, the maximum current density increased by 48.90% with the addition of sodium chloride. Due to the limitation of anode proton transfer, which constrained electricity production, 50 mM PBS was added to regulate the biofilm pH and increase the conductivity, and a 2.1-fold increase in the current density was obtained relative to the current density in Period 1. Torres et al. showed that current was linearly proportional to the concentration of the buffer solution at a constant potential [16]. The accurate injection of different concentrations of buffer solution to control the anode biofilm pH could be investigated in future studies.

4. Conclusions

In this study, the maximum current density, coulombic efficiency, and COD removal rate were obtained with an electrode spacing of 20 cm. The variation in DO between the anode and cathode for different electrode spacings was significantly negatively correlated with the difference in voltage. A comparatively low COD concentration (200 mg/L) helped to enhance electricity generation. Furthermore, 50 mM PBS added to the SW decreased the internal resistance and ohmic loss. To increase the use of CW-MFCs, optimum electrode materials for enhancing catalytic activities and electron transfer properties should be investigated in future studies.

Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 41571476), National Key Technologies R&D Program of China (Grant No. 2015BAL02B01-02), Provincial Key Technologies R&D Program of Jiangsu, China (BE2015358) and Scientific Research Foundation of Graduate School of Southeast University (Grant No. YBJJ1647).

Author Contributions

The manuscript was written by Hailiang Song and Shuai Zhang but all the authors contributed to its preparation and review. Experiments were performed by Wenli Xiang. Data analyses were carried out by Xizi Long and Xiaoli Yang in discussion with Hua Li.

Conflicts of Interest

The authors declare no conflict of interest.

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Figure 1. Schematics of the vertical subsurface-flow wetland-coupled microbial fuel cell systems (VSFCW-MFCs). (a) Experimental set-up and (b) VSFCW-MFCs with distances ranging from 10 to 40 cm between the electrodes.
Figure 1. Schematics of the vertical subsurface-flow wetland-coupled microbial fuel cell systems (VSFCW-MFCs). (a) Experimental set-up and (b) VSFCW-MFCs with distances ranging from 10 to 40 cm between the electrodes.
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Figure 2. Effects of dissolved oxygen (DO) on voltage in VSFCW-MFCs. (a) Average voltages of different electrode spacings in the experimental system (mean ± SE, n = 4); (b) Variation in DO concentration from the bottom to the top of the reactors (mg/L) (mean ± SE, n = 4); (c) Correlation of voltage with DO concentration at a single time point in the four reactors (The voltage and DO measurement was taken at three time points respectively).
Figure 2. Effects of dissolved oxygen (DO) on voltage in VSFCW-MFCs. (a) Average voltages of different electrode spacings in the experimental system (mean ± SE, n = 4); (b) Variation in DO concentration from the bottom to the top of the reactors (mg/L) (mean ± SE, n = 4); (c) Correlation of voltage with DO concentration at a single time point in the four reactors (The voltage and DO measurement was taken at three time points respectively).
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Figure 3. Polarization curves and power density–current density curves for different electrode spacings in VSFCW-MFCs.
Figure 3. Polarization curves and power density–current density curves for different electrode spacings in VSFCW-MFCs.
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Figure 4. Chemical oxygen demand (COD) removal efficiencies of different electrode spacings in VSFCW-MFCs (mean ± SE, n = 4).
Figure 4. Chemical oxygen demand (COD) removal efficiencies of different electrode spacings in VSFCW-MFCs (mean ± SE, n = 4).
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Figure 5. COD removal along the VSFCW-MFC with a 20-cm electrode spacing at different influent COD concentrations (mean ± SE, n = 4). PBS and glucose were added to make SW with CODs of 200, 400, and 800 mg/L with organic loadings of 0.30, 0.71, and 1.38 kg COD/(m3·d), respectively. The gray area represents the projected anode area.
Figure 5. COD removal along the VSFCW-MFC with a 20-cm electrode spacing at different influent COD concentrations (mean ± SE, n = 4). PBS and glucose were added to make SW with CODs of 200, 400, and 800 mg/L with organic loadings of 0.30, 0.71, and 1.38 kg COD/(m3·d), respectively. The gray area represents the projected anode area.
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Figure 6. Electrical performance with the addition of PBS to the VSFCW-MFC (Period 1, SW was mixed with 50 mM PBS to enhance the conductivity and buffer capability; Period 2, only SW was fed into the system; Period 3, sodium chloride was added to improve the conductivity of the SW). (a) Evolution of voltage against time. (b) Polarization curves and power density–current density curves.
Figure 6. Electrical performance with the addition of PBS to the VSFCW-MFC (Period 1, SW was mixed with 50 mM PBS to enhance the conductivity and buffer capability; Period 2, only SW was fed into the system; Period 3, sodium chloride was added to improve the conductivity of the SW). (a) Evolution of voltage against time. (b) Polarization curves and power density–current density curves.
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Table 1. Concentrations and components of synthetic domestic wastewater in the VSFCW-MFCs.
Table 1. Concentrations and components of synthetic domestic wastewater in the VSFCW-MFCs.
First Trial: Synthetic Wastewater (SW) with Phosphate Buffer Solution (PBS)Second Trial: SW without PBSThird Trial: SW without PBS
IngredientConcentration (mg/L)IngredientConcentration (mg/L)IngredientConcentration (mg/L)
Glucose200.00Glucose200.00Glucose200.00
Carbamide10.00KNO3252.50KNO3252.50
NH4Cl310.00KH2PO426.32KH2PO426.32
NaH2PO44970.00NaHCO3336.00NaHCO3336.00
Na2HPO42750.00MgSO4·7H2O200.00NaCl330.00
KCl130.00CaC1215.00MgSO4·7H2O200.00
NaHCO33130.00FeC13·6H2O1.00CaC1215.00
(NH4)2SO4560.00MnSO4·H2O28.00FeC13·6H2O1.00
MgSO4·7H2O200.00CoCl2·6H2O2.40MnSO4·H2O28.00
CaC1215.00Na2Mo4·2H2O0.40CoCl2·6H2O2.40
FeC13·6H2O1.00Na2Mo4·2H2O0.40
MnSO4·H2O28.00
CoCl2·6H2O2.40
Na2Mo4·2H2O0.40
Table 2. Energy output of the VSFCW-MFCs. (mean ± SE, n = 4).
Table 2. Energy output of the VSFCW-MFCs. (mean ± SE, n = 4).
Electrode Spacing (cm)Current Generation (mA)Power Density (W/m3)COD Removal (%) in EffluentCoulombic Efficiency (%)
100.44 ± 0.050.09 ± 0.0189.60 ± 3.210.26 ± 0.03
200.56 ± 0.060.15 ± 0.0294.90 ± 2.440.31 ± 0.02
300.52 ± 0.040.13 ± 0.0293.31 ± 2.750.30 ± 0.03
400.42 ± 0.040.08 ± 0.0185.42 ± 3.540.26 ± 0.01
Table 3. VSFCW-MFC energy extraction with different influent COD concentrations (mean ± SE, n = 4). PBS and glucose were added to make SW with CODs of 200, 400, and 800 mg/L with organic loadings of 0.30, 0.71, and 1.38 kg COD/(m3·d).
Table 3. VSFCW-MFC energy extraction with different influent COD concentrations (mean ± SE, n = 4). PBS and glucose were added to make SW with CODs of 200, 400, and 800 mg/L with organic loadings of 0.30, 0.71, and 1.38 kg COD/(m3·d).
Substrate Concentration (mg/L)Bv (kg COD/ (m3·NAC·d))Eoc (mV)Eoc,an (mV)Eoc,cat (mV)Pmax (W/m3)Current Generation (mA)COD Removal (%)Coulombic Efficiency (%)
Glucose COD = 200296.88 ± 26.45741 ± 34−500 ± 39241 ± 280.20 ± 0.020.49 ± 0.0490.45 ± 4.141.30 ± 0.23
Glucose COD = 400706.05 ± 38.62698 ± 57−511 ± 47187 ± 240.17 ± 0.020.45 ± 0.0390.99 ± 3.520.50 ± 0.04
Glucose COD = 8001378.17 ± 121.23627 ± 48−526 ± 61101 ± 130.13 ± 0.01 0.40 ± 0.0390.76 ± 3.340.23 ± 0.03
Notes: Bv is the COD loading of the anode working volume; Eoc represents the open circuit voltage; Eoc,an is the open circuit potential of the anode; Eoc,cat is the open circuit potential of the cathode; Pmax is the maximum power density calculated at an external resistance of 400 Ω; and current density and coulombic efficiency are consistent with Pmax.

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Song, H.; Zhang, S.; Long, X.; Yang, X.; Li, H.; Xiang, W. Optimization of Bioelectricity Generation in Constructed Wetland-Coupled Microbial Fuel Cell Systems. Water 2017, 9, 185. https://doi.org/10.3390/w9030185

AMA Style

Song H, Zhang S, Long X, Yang X, Li H, Xiang W. Optimization of Bioelectricity Generation in Constructed Wetland-Coupled Microbial Fuel Cell Systems. Water. 2017; 9(3):185. https://doi.org/10.3390/w9030185

Chicago/Turabian Style

Song, Hailiang, Shuai Zhang, Xizi Long, Xiaoli Yang, Hua Li, and Wenli Xiang. 2017. "Optimization of Bioelectricity Generation in Constructed Wetland-Coupled Microbial Fuel Cell Systems" Water 9, no. 3: 185. https://doi.org/10.3390/w9030185

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