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Life 2013, 3(2), 308-320; doi:10.3390/life3020308

Hot Spring Metagenomics

Departamento de Bioloxía Celular e Molecular, Facultade de Ciencias, Universidade da Coruña, 15071 A Coruña, Spain
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Received: 7 February 2013 / Revised: 11 April 2013 / Accepted: 15 April 2013 / Published: 25 April 2013
(This article belongs to the Special Issue Extremophiles and Extreme Environments)
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Abstract

Hot springs have been investigated since the XIX century, but isolation and examination of their thermophilic microbial inhabitants did not start until the 1950s. Many thermophilic microorganisms and their viruses have since been discovered, although the real complexity of thermal communities was envisaged when research based on PCR amplification of the 16S rRNA genes arose. Thereafter, the possibility of cloning and sequencing the total environmental DNA, defined as metagenome, and the study of the genes rescued in the metagenomic libraries and assemblies made it possible to gain a more comprehensive understanding of microbial communities—their diversity, structure, the interactions existing between their components, and the factors shaping the nature of these communities. In the last decade, hot springs have been a source of thermophilic enzymes of industrial interest, encouraging further study of the poorly understood diversity of microbial life in these habitats.
Keywords: metagenomics; hot springs; thermophiles metagenomics; hot springs; thermophiles
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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López-López, O.; Cerdán, M.E.; González-Siso, M.I. Hot Spring Metagenomics. Life 2013, 3, 308-320.

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