Phenotypic MicroRNA Microarrays
AbstractMicroarray technology has become a very popular approach in cases where multiple experiments need to be conducted repeatedly or done with a variety of samples. In our lab, we are applying our high density spots microarray approach to microscopy visualization of the effects of transiently introduced siRNA or cDNA on cellular morphology or phenotype. In this publication, we are discussing the possibility of using this micro-scale high throughput process to study the role of microRNAs in the biology of selected cellular models. After reverse-transfection of microRNAs and siRNA, the cellular phenotype generated by microRNAs regulated NF-κB expression comparably to the siRNA. The ability to print microRNA molecules for reverse transfection into cells is opening up the wide horizon for the phenotypic high content screening of microRNA libraries using cellular disease models.
Scifeed alert for new publicationsNever miss any articles matching your research from any publisher
- Get alerts for new papers matching your research
- Find out the new papers from selected authors
- Updated daily for 49'000+ journals and 6000+ publishers
- Define your Scifeed now
Kwon, Y.-J.; Heo, J.Y.; Kim, H.C.; Kim, J.Y.; Liuzzi, M.; Soloveva, V. Phenotypic MicroRNA Microarrays. Microarrays 2013, 2, 63-80.
Kwon Y-J, Heo JY, Kim HC, Kim JY, Liuzzi M, Soloveva V. Phenotypic MicroRNA Microarrays. Microarrays. 2013; 2(2):63-80.Chicago/Turabian Style
Kwon, Yong-Jun; Heo, Jin Y.; Kim, Hi C.; Kim, Jin Y.; Liuzzi, Michel; Soloveva, Veronica. 2013. "Phenotypic MicroRNA Microarrays." Microarrays 2, no. 2: 63-80.