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Agriculture 2013, 3(3), 536-555; doi:10.3390/agriculture3030536
Abstract: Proliferative enteropathy, commonly known as “ileitis” continues to be a significant production-limiting disease in pig herds throughout the world. The disease can be controlled with a combination of vaccination and antibiotic medication. However, pressure from consumers to reduce antibiotic use in livestock industries highlights the need to better understand the epidemiology of ileitis, the mechanisms of immunity, and to identify management factors that can reduce the load of Lawsonia intracellularis in both pigs and the environment. New diagnostic assays and economic modelling of ileitis will help producers target optimal treatment times and minimize the production losses associated with ileitis. This review aims to outline the current advances in disease diagnosis, epidemiology, control strategies and the economic impact of both clinical and sub-clinical disease.
Ileitis is the common name for the wasting disease in pigs more accurately named porcine proliferative enteropathy (PPE). Clinical signs include reduced weight gains and diarrhea in growing pigs. Finisher or breeding animals can be affected with the more acute proliferative hemorrhagic enteropathy (PHE), losing blood in their feces due to intestinal hemorrhage. The proliferative enteropathies encompass a group of conditions (necrotic enteritis, regional ileitis, PHE and porcine intestinal adenomatosis) with similar pathogenesis. Gross pathology presents as a thickened mucosa in the distal small intestine and proximal large intestine. Histopathology is characterized by proliferation of crypt enterocytes, loss of goblet cells and intracellular bacteria in the apical cytoplasm of proliferating enterocytes . The obligate intracellular bacterium, Lawsonia intracellularis, is the etiologic agent of all forms of PPE .
The characteristic gross and histological lesions of proliferative enteropathy (PE) were first described in North American weaned pigs in 1931 . Subsequent reports described lesions of PPE in Europe, Asia, Australasia and America over the next 40 years [4,5,6,7,8,9,10]. However it was not until 1973 that the presence of intracellular bacteria within proliferative lesions was first described . It took another 20 years for the etiologic agent, L. intracellularis, to be cultured in mammalian cells and for Koch’s postulates to be fulfilled with the reproduction of PE in pigs [2,12].
Pigs of all ages, and pigs housed in a range of management systems, including farrow to finish farms and multiple site production farms are affected with PE. However, it is predominantly in grower and finisher pigs where disease control strategies are focused. Antibiotics were commonly used to treat or prevent PE, and practices developed where pigs were medicated continuously from about nine weeks of age until slaughter. While no data has been published to suggest that L. intracellularis develops resistance to commonly used antibiotics, the overuse of antibiotics could lead to antibiotic resistance in other bacteria. The aim of this review is to detail the advances in PE epidemiology, diagnosis and control strategies that will help reduce the reliance on antibiotics to control PE.
2. The Prevalence of Proliferative Enteropathy
The estimated prevalence of disease and L. intracellularis infection depends on the diagnostic method used. Slaughter checks relying on gross pathology have suggested that somewhere between 5% and 20% of pigs are affected with PE [13,14,15,16,17]. However, slaughter checks significantly underestimate the incidence of PE in grower pigs because PE lesions resolve within two to four weeks of clinical signs being present . Producers and veterinarians surveyed in the UK and Australia estimated that clinical disease was prevalent in somewhere between 30% and 56% of herds [19,20]. Serological surveys of finisher pigs estimated that more than 80%, and up to 100% of pigs are infected with L. intracellularis [21,22,23]. In contrast, detection of L. intracellularis DNA in feces (by polymerase chain reaction) suggests that between 4.5% and 45% of herds are infected with L. intracellularis and the prevalence of infected animals ranges between 3.3% and 12% [24,25,26,27,28,29]. The diagnostic tests also vary in their ability to diagnose clinical disease, sub-clinical disease or L. intracellularis infection. The ability to interpret the results of infection prevalence studies in herds will help producers target disease control strategies to the right age group of pigs, and reduce the unnecessary use of antibiotics. Strategies to ensure the rational use of antibiotics are outlined in section 4.1.
3. Diagnosis of Proliferative Enteropathy
Early diagnosis of PE relied on gross pathology at the abattoir (thickening of the ileal mucosa) and histopathology; visualizing the proliferative epithelium and intracellular curved bacteria in the apical cytoplasm of cells with a Warthin Starry silver stain, and later with a specific monoclonal antibody to L. intracellularis [30,31]. Necropsies to diagnose PE on farm were only undertaken on sick pigs, not expected to recover, so other diagnostics were needed. The development of ante-mortem diagnostic assays for L. intracellularis infection was aided by the culture of L. intracellularis.
3.1. Serological Assays
Relatively impure L. intracellularis proteins, extracted from PE affected tissue, were initially used in both an ELISA and an indirect fluorescent antibody test (IFAT) to detect serum IgG antibodies to L. intracellularis [32,33]. The IFAT was able to overcome high background problems observed in the early ELISA by visualizing specific antigen-antibody reactions under the microscope, allowing differentiation between fluorescing L. intracellularis and non-specific reactions. Culture of L. intracellularis allowed the development of whole cell immunoperoxidase antibody tests (IPMA)  and ELISA tests using L. intracellularis outer membrane proteins or lipopolysaccharides (LPS) as antigens [35,36], but most of these assays have remained as research tools. A blocking ELISA for serum IgG to L. intracellularis (bioScreen Ileitis antibody ELISA; Munster, Germany) is now commercially available and routinely used to determine the timing and prevalence of L. intracellularis infection. Serology can help producers and veterinarians identify risk factors and the efficacy and optimal timing for PE control measures including vaccination, medication and improved hygiene [37,38,39]. The sensitivity of this blocking ELISA was between 72% and 90.5% and the specificity was between 83% and 93%, using sera from both field and experimental challenge trials [40,41]. Regardless of which serological assay is used, L. intracellularis antibodies are not routinely detected until about 21 days post challenge, after clinical signs and fecal shedding are already observed, so serology is an indicator of previous infection [42,43]. The IFAT, IPMA and LPS ELISA have been modified to measure L. intracellularis specific IgA and IgM antibodies in sera and in mucosal scrapings, so they continue to be used to elucidate the immune response to both vaccination and virulent challenge [44,45,46].
3.2. Molecular Assays
The development of DNA based diagnostic assays such as hybridization and the polymerase chain reaction (PCR) did not rely upon the culture of L. intracellularis. Hybridization probes were produced from short fragments of L. intracellularis DNA that were cloned  and used to detect L. intracellularis DNA in pig faeces. Later, short fragments of L. intracellularis DNA were amplified by PCR using oligonucleotides synthesized to detect L. intracellularis DNA [48,49,50,51]. The relatively short duration of L. intracellularis excretion (up to three weeks) and the higher cost of PCR (compared to serology) has meant that the PCR has been used primarily to detect infection in diarrheic pigs, and is less useful for monitoring PE control in herds. The PCR assay cannot discriminate between live and dead L. intracellularis in feces, so some reports of PCR positive pigs detected at a single time point make it difficult to interpret whether the pigs were actively infected at the time or if L. intracellularis was transiting through their digestive tracts.
Although fecal PCR is both more sensitive and specific than other diagnostic assays, it was not quantitative until recently, partly because the amplification of L. intracellularis DNA can be inhibited by the presence of large amounts of competing non-target DNA , bile salts and bilirubin in feces . The interpretation of fecal PCR results has also been confused by the sensitivity of the assay. Detection of L. intracellularis DNA does not prove that L. intracellularis is the cause of diarrhea. The development of a multiplex PCR assay that can detect L. intracellularis and Brachyspira spp. in the same sample helped identify multiple causes of diarrhea in the same animal [54,55].
Recently a number of quantitative real time PCR (qPCR) assays have been developed for L. intracellularis [56,57,58,59]. Sensitivity of these new assays was aided by advances in DNA extraction protocols with magnetic particle processors capable of capturing nucleic acids and removing them from inhibitors in the feces. These qPCR assays can calculate L. intracellularis numbers in feces relative to standards seeded with known numbers of L. intracellularis, providing a reproducible linear range of 108 to 104 bacteria/g feces. The number of L. intracellularis excreted in pig feces (determined by qPCR) is between 104 and 108 L. intracellularis per gram of feces depending on whether pigs are clinically or sub-clinically affected with PE [56,57,58,59], with clinically affected pigs excreting more than 107 L. intracellularis per gram of faeces.
L. intracellularis excretion levels correlated well with antibody titres, the severity of histopathology lesions, the duration of L. intracellularis excretion and the severity of diarrhea [60,61]. A weaker inverse correlation exists between the qPCR and production losses associated with PE, which may be explained by other factors that impact on pig growth. However, a one log10 unit increase in the number of L. intracellularis increases the risk for low growth rate by two times . The critical number or threshold of L. intracellularis that causes reduced growth rates in experimentally infected pigs is reported to be above 107 L. intracellularis per gram of feces . Once the number of L. intracellularis shed by pigs increased from 107 to 108 per gram of feces, average daily gain was reduced by 131 g/day. Identifying a critical threshold of infection across a wide range of commercial management systems will allow producers to use the qPCR to target ileitis treatment options.
Preliminary studies have indicated that fecal samples can be pooled prior to qPCR analysis to help reduce costs and to encourage producers to use the qPCR as a herd health monitoring test. Theoretical pooling of samples indicated that the qPCR was still accurate when pools of 10 individual samples were used . However, pools of five fecal samples provided more accurate results than pools of 10 feces when the qPCR assays were performed .
The diagnosis of L. intracellularis infection in real time by qPCR enables producers to quantify the efficacy of PE control measures, as reduced numbers of L. intracellularis correlate with reduced severity of PE. Data from experimental challenge trials shows the significant reduction in the number of L. intracellularis shed in pigs vaccinated with Enterisol® Ileitis or medicated with antibiotics [46,65]. The qPCR can also be used to quantify the efficacy of disinfection and hygiene protocols  and the biosecurity risk associated with rodents near piggeries . Avoiding the over-use of antibiotics for the control of PE relies on the correct interpretation of all of the new diagnostic assays. Quantitative assays identify the severity of disease and help producers decide on what level of intervention is needed to control PE. Qualitative assays such as serology provide producers with information on the optimal timing of disease control measures and the prevalence of exposure to L. intracellularis in the herd.
4. Control of Ileitis
The development of experimental PE challenge models and diagnostics to monitor L. intracellularis infection enabled antibiotic efficacy studies to be performed in the absence of concurrent infections commonly found on farm. Prior to this, veterinarians relied on monitoring clinical signs of PE or pathology at slaughter to determine whether treatment strategies were effective. However, diarrhea and poor growth could be caused by other factors and suspect gross pathology at abattoir was only proven by histopathology in less than 11% of cases . These new disease challenge models enabled the more rational use of antibiotics for PE control, and allowed evaluation of other potential control strategies including immunization and improved hygiene.
4.1. Antibiotic Medication
The efficacy of antibiotics such as olaquindox, to control PE was originally identified by field veterinarians trying to control outbreaks of PE . Later experimental challenge trials demonstrated that high doses of tiamulin, tylosin, chlortetracycline, lincomycin and olaquindox were able to treat pigs with PE, reducing clinical signs, histological lesions of PE and the duration of fecal shedding of L. intracellularis [67,68,69,70,71,72,73,74]. High doses of tiamulin, tylosin, olaquindox and chlortetracycline could also prevent L. intracellularis infection if given continuously in feed [67,70,74]. However, once these antibiotics were removed, the pigs remained immunologically naïve and were susceptible to a later L. intracellularis challenge . In addition, continuous use of antibiotics may increase the potential for other bacteria to develop resistance.
Public concern over the development of antibiotic resistance by bacteria led to significant pressure world-wide to find alternative strategies to control PE and to reduce the prophylactic use of antibiotics. Protective immunity to PE was first demonstrated in a minimal disease piggery with two sequential outbreaks of the hemorrhagic form of ileitis (PHE). Pigs that survived the first outbreak were protected from a subsequent disease outbreak . The authors proposed that immunity could be induced by bringing young animals into contact with the source of infection for three weeks, prior to the addition of antibiotics to terminate infection.
Protective immunity was later demonstrated in experimental challenge models, where pigs previously challenged orally with L. intracellularis were protected from re-infection on a subsequent challenge [43,45,77]. The failure to detect L. intracellularis in the feces of re-challenged pigs by PCR indicated that virulent, live L. intracellularis in the second inoculum were not able to colonize mucosal cells in previously challenged pigs. Therapeutic strategies were designed to allow L. intracellularis infection and immunity to re-infection, with antibiotics used to avoid clinical disease. Antibiotics were removed from pig diets for 12 to 18 days and then pulsed in feed or in water at high doses for two to four days every two to three weeks to suppress disease. The period without antibiotics allowed pigs to become infected with L. intracellularis but also to develop immunity to re-infection .
Antibiotics, along with extensive cleaning of pens and depopulation of younger pigs were also used in attempts to eradicate PE, by eliminating L. intracellularis from the pig and the environment [79,80,81,82]. Eradication of clinical signs of PE appeared possible on smaller farms, but very few studies were able to demonstrate complete eradiation of L. intracellularis from the herd and the environment over time. Biosecurity needed to be maintained at a very high level to ensure continued eradication of L intracellularis . Outbreaks of severe disease often followed when L. intracellularis was accidentally introduced into a naïve herd .
4.2. Improved Hygiene
L. intracellularis can survive (outside of the pig) in conventional pens for at least two weeks at temperatures between 9 °C and 18 °C [39,50]. Management practices that improve biosecurity and hygiene, including all-in-all-out production and minimal mixing of pigs, are associated with a lower risk of L. intracellularis infection [83,84,85]. The housing and flooring in pens may also impact on the survival of L. intracellularis. It may be expected that pigs reared on deep litter systems would be exposed to greater numbers of pathogens, including L. intracellularis, because deep litter systems are only cleaned and/or disinfected between batches of pigs, compared with conventional pens where manure is removed more regularly. However, pigs grown out on deep litter systems had a similar prevalence of L. intracellularis infection as cohorts raised on traditional concrete-based systems , and grower and finisher pigs reared on partially slatted floors had a higher risk of diarrhea than pigs housed on solid concrete floors . Power hosing concrete slatted floors with cold water, followed by disinfection with potassium peroxymonosulfate (Virkon S) and drying time was able to significantly reduce the survival and transmission of L. intracellularis from the environment to naïve pigs . The efficacies of a wide range of other disinfectants against L. intracellularis have been tested by in vitro culture methods. The iodophore povidone iodine and quaternary ammonium compounds were both effective against L. intracellularis in in vitro studies, in the absence of faecal material [50,88]. However, the efficacy of disinfectants needs to be studied in pig pens with residual manure, which can inactivate the disinfectant. Reducing the number of L. intracellularis in pens between batches of pigs reduces the severity of disease in subsequent batches, but the aim is not to remove all L. intracellularis, or naïve herds may develop, which are highly susceptible to outbreaks of PHE, the more acute form of PE.
4.3. Vaccination and Induced Immunity
Oral vaccination with a commercial avirulent live L. intracellularis vaccine (Enterisol® Ileitis) protects pigs from clinical disease; significantly reducing microscopic lesions of PE following a virulent heterologous L. intracellularis challenge . Vaccination did not completely prevent fecal shedding of L. intracellularis following virulent challenge, but did significantly reduce the number of L. intracellularis shed in feces and the duration of shedding in vaccinated pigs [42,89,90]. In many commercial herds antibiotic medication was no longer required to control PE in vaccinated herds. However, producers believed that in some straw-bedded systems L. intracellularis infection was harder to suppress with vaccination alone . In these cases, antibiotics were used in tandem with vaccination.
As expected for an intracellular bacterium, L. intracellularis infection induces both specific humoral (IgA, IgG) and cell mediated (IFN-γ) immune responses in serum and the intestinal mucosa. The immune response to both natural challenge and oral vaccination is dose-dependent [43,44,45,46]. The absence of an anamnestic serum IgA response in re-challenged pigs, along with an increased gamma IFN response, led to the speculation that cell-mediated immune responses alone were likely to be mediators of protective immunity to L. intracellularis . However, accumulation of IgA in proliferating crypt cells and in the cell debris of the crypt lumen of PE-affected pigs, where L. intracellularis also reside , suggests that IgA is also involved in a protective immune response to L. intracellularis. Induction of mucosal immunity is believed to depend upon presentation of the antigen at the mucosal surface, but protection against other intracellular pathogens (Salmonella enteriditis) following systemic vaccination  suggest that systemic vaccination may protect pigs from L. intracellularis challenge. In early studies, disease severity was significantly reduced in pigs vaccinated twice intramuscularly (IM) with L. intracellularis bacterin or recombinant GroEL-like protein . More recently, IM vaccinations, with L. intracellularis bacterin or recombinant outer membrane proteins of L. intracellularis (19/21 and 37 kDa antigens), protected pigs from PE . In comparative studies with the commercial live L. intracellularis vaccine (Enterisol® Ileitis), vaccination (10-times dose) administered orally or intra-muscularly induced a similar protective immune response, with systemic and mucosal L. intracellularis-specific antibodies and mucosal cytokines . These inactivated L. intracellularis vaccines appear to protect against infection if sufficient L. intracellularis antibodies are present to prevent infection at the mucosal. Alternate adjuvants or higher doses of L. intracellularis antigens may increase the efficacy of systemic vaccination routes. To date no detectable immune marker for protection has been identified, so veterinarians have to rely on detection of exposure to L. intracellularis (serum IgG antibodies) as a proxy for immunity.
The need to remove all medication for three days before and after vaccination was initially seen as an obstacle to the uptake of an oral vaccine . The vaccine was registered for weaner pigs that may have concurrent infections requiring antibiotic medication. While vaccination pre-weaning may overcome this obstacle, the potential for maternal antibodies to interfere with vaccination was a concern . While maternally acquired antibodies are vital in protecting piglets from pathogen invasion, they may also interfere with oral vaccination. Maternal antibodies ingested by piglets bind to antigens found in the intestinal lumen, form complexes and are then internalized by antigen-presenting cells. Both B and T cells are stimulated in the piglet, though B cell stimulation is inhibited. Modifying the vaccination route may overcome the potential interference of maternal antibodies. Earlier studies demonstrated that IM vaccination could induce a protective immune response in L. intracellularis challenged pigs [46,94,95]. However, the Enterisol® Ileitis vaccine is not registered for this use.
5. Production and Economic Impacts of Ileitis
The clinical impact of L. intracellularis infection depends on the dose of bacteria ingested by pigs [43,96], but also on the expression of bacterial virulence genes , stimulation of the host’s immune system  and environmental factors. The pig’s diet, genetics, immune response, intestinal microflora and intestinal health are all likely to impact on the severity of disease.
Clinical signs of ileitis including diarrhea are evident for one to three weeks, reducing feed conversion efficiency (FCE) by up to 50% . Most pigs recover from clinical disease within four to six weeks but chronic disease can affect pig growth over 10 weeks. Significant variation in weight gains has also been observed in pigs affected by PE, with reductions in average daily gain (ADG) ranging from 17% to 84% of the gain of unaffected pigs . This variation in growth within a batch of pigs can lead to increased days to slaughter or increased back fat on larger animals and ultimately economic losses for the producer . Simulation of two different clinical outbreaks of PE, (reduced feed intake, ADG, feed conversion efficiency, increased P2 back fat) indicated that clinical ileitis costs between $7 and $13 AUD for every pig [100,101].
Many pigs are sub-clinically affected, as demonstrated by the extremely high prevalence of seropositive pigs relative to the small proportion of pigs with PE lesions [17,21,23]. Diarrhea is rarely observed in pigs sub-clinically affected with PE, but reductions in ADG between 9% and 42%, and reduced FCE between 6% and 37% have been reported [99,102]. The economic impact of sub-clinical PE is difficult to estimate because many producers are unaware that sub-clinical PE is present in their herd. Experimental reproduction of sub-clinical ileitis in a group of grower pigs caused significantly decreased feed intake (4 kg) over a three week period, but did not reduce pig weights or weight gains in the same period, though variation in pig weights was significantly increased in infected pigs relative to non-infected pigs . In the three weeks after infection peaked, L. intracellularis infected pigs increased weight more quickly than in the early infection period, but muscle deposition was significantly reduced . This may in part explain the increased P2 back fat depth reported in previous studies, although no significant differences in body composition, measured with a CT scanner, were observed between sub-clinically and uninfected animals . Economic modeling of these production losses on a herd basis in the absence of control strategies resulted in an $8.33 AUD reduction in revenue per pig in a herd with 80% of pigs sub-clinically affected .
6. Epidemiology of Ileitis
6.1. Transmission of Infection between Pigs and the Environment
The occurrence of PE in segregated early weaning systems indicated that pigs are either infected from the sow pre-weaning, from the environment, or from contact with pigs or other species of animals, birds or insects. Transmission of infection between in-contact pigs was demonstrated in natural outbreaks of PE when animals were recently mixed, or when new animals were introduced [6,10] and also in experimental challenge trials [50,105]. The transmission of L. intracellularis to naïve pigs is primarily via the ingestion of contaminated feces. Pigs affected with PE shed large numbers of L. intracellularis, enough to infect naïve pigs in contact with them [43,50,105,106]. Epidemiology studies have identified frequent mixing of pigs, the introduction of new pigs and continuous flow production systems as risk factors for PE [22,87].
The detection of L. intracellularis in pig feces coincides with the presence of clinical signs of PE [43,107]. However, in some experimental challenge trials, pigs continued to shed L. intracellularis after clinical signs of PE ceased, [42,108], suggesting a carrier state similar to Salmonella infections, where stressed carrier pigs re-commence excretion of Salmonella . L. intracellularis bacteria were found in the tonsils of pigs clinically affected with PE , however, a carrier state of L. intracellularis infection has not been demonstrated. Pigs allowed to recover naturally from PE do not continue to shed detectable numbers of L. intracellularis, and are not able to transmit infection to naïve, in-contact pigs . Treatment of recovered pigs with the corticosteroid dexamethasone likewise did not lead to detectable L. intracellularis excretion, or transmission of infection to naïve in-contact pigs. The development of immunity to L. intracellularis infection, even when pigs were challenged with a much higher secondary dose (increased by four logs), also indicates that L. intracellularis does not readily reappear from a carrier state [43,90].
Elimination of L. intracellularis from pigs and the environment is important in reducing the transmission of infection to naïve pigs. Chlortetracycline in feed (400 ppm) was able to rapidly terminate L. intracellularis excretion relative to tiamulin (200 ppm), oxytetracycline (400 ppm) and non-medicated pigs .
6.2. Transmission of L. intracellularis from Sows to Their Progeny
The detection of L. intracellularis in the feces of a small number of suckling pigs in endemically-infected herds has led researchers to speculate that sows may be a source of infection to their piglets [108,112,113]. However, the detection of antibodies to L. intracellularis in three to six week old pigs, and the occurrence of PE predominantly in pigs greater than six weeks of age , suggests that piglets are more likely protected from L. intracellularis by either antibodies or lymphocytes from the sow in colostrum or later by IgA in milk. In late lactation, circulating antibodies in the sow are concentrated into pre-colostrum, along with circulating IgA-secreting lymphocytes (plasma calls) which “home” to the mammary gland . Piglets can passively absorb maternal antibodies during the first 24 to 36 h of life . Lymphocytes from the birth sow can be absorbed by her piglets in the first week of life .
The ability of sows to protect piglets against L. intracellularis infection will therefore depend on the level of circulating antibodies in the sow and the transfer of protective immune secretions and cells to her progeny. Three week old piglets suckling on a recently infected sow were immune to challenge with L. intracellularis, in contrast to their weaned litter mates that were infected with the same L. intracellularis challenge . Four weeks after the protected piglets were weaned they were susceptible to a virulent challenge; whereas their previously infected litter mates were now immune. This passive protection did not persist after pigs were weaned. Sows with no detectable L. intracellularis antibodies at farrowing, but from an endemically infected herd, were only able to partially protect their suckling progeny. Clinical signs were reduced, but piglets excreted L. intracellularis and raised significantly reduced serum IgG titres to L. intracellularis . The ability of sows with higher L. intracellularis antibody titres to protect their suckling piglets was also demonstrated in field studies. The progeny of 20 seropositive and 20 seronegative gilts were challenged with L. intracellularis at two weeks of age and remained suckling for an additional three weeks. Only 21% of piglets on seropositive gilts had L. intracellularis antibodies three weeks post challenge, relative to 86% of piglets from seronegative gilts .
6.3. External Vectors in Transmission of L. intracellularis
The main source of L. intracellularis in the piggery is infected pigs, and transmission is via ingestion of contaminated feces [50,105]. However, outbreaks of PE occur in naïve herds, suggesting that other sources of L. intracellularis may exist. A wide range of domestic and wildlife species can become infected with L. intracellularis and some also develop PE [118,119,120,121,122,123,124,125,126]. Birds, insects, rodents and feral pigs are considered the most likely external vectors for PE in pigs due to their proximity to commercial pig units. L. intracellularis infection of rodents has been investigated in both field and experimental challenge studies. Laboratory rats and mice challenged with porcine strains of L. intracellularis have been infected, raised persistent immune responses and developed caecal and colonic lesions of PE [58,127,128,129]. In addition, L. intracellularis DNA was detected in the intestinal tissue of wild mice and rats trapped on pig farms where PE was endemic [58,130]. A high proportion of wild rats trapped on pig farms with endemic PE were excreting L. intracellularis (≥70.6%), but more significantly, a small proportion of these rats excreted extremely high numbers of L. intracellularis (1010/g of feces) . Rodents may therefore be an important reservoir of L. intracellularis on pig farms, as less than 1 g of rodent feces would need to be ingested by a naïve pig for transmission of infection to occur.
To date the only report of L. intracellularis infection in birds relates to ratites including the ostrich and emu [123,124]. As these species are not commonly associated with pig herds, they are unlikely to pose a significant infection risk. Experimental challenge of sparrows with porcine isolates of L. intracellularis failed to demonstrate colonization or disease associated with L. intracellularis . Conversely, invertebrates collected from a geographical spread of UK pig herds indicated that L. intracellularis DNA was frequently detected in house and hover flies, but not in cockroaches . Small differences in DNA repeats (four microsatellite markers) indicated that the L. intracellularis isolate found in pigs couldn’t be differentiated from the fly isolate. However, it’s not possible to say whether the flies are just a measure of L. intracellularis infection in the herd or a potential source of infection to naïve pigs.
Veterinarians and pig producers have benefitted from the recent knowledge gained in the diagnosis of L. intracellularis infection, transmission and survival of L. intracellularis and PE control strategies. This also translates to benefits for society, as these recent advances will enable producers to control PE with a reduced reliance on antibiotics.
The development of quantitative diagnostic assays enable producers to measure the severity of L. intracellularis infection in pig herds in real time, providing them with opportunities to fine tune treatments and ultimately reduce costs associated with disease control. In the absence of an immune correlate for protection, producers should continue to regularly monitor their herds by serology to ensure that L. intracellularis infection is widespread, as a proxy for immunity.
Knowledge of the potential sources of L. intracellularis in pigs, external vectors and the environment enables producers and veterinarians to develop hygiene and biosecurity procedures, including rodent eradication programs, to prevent outbreaks of PE. Management strategies that reduce the spread and number of L. intracellularis in the pig herd include all-in-all-out production, quarantine areas for newly introduced pigs, minimal mixing of pigs and effective disinfection of pens or sheds between batches.
Vaccination, either with the current live oral vaccine or with a new recombinant protein vaccine given IM, will ensure that all pigs are exposed to L. intracellularis, and will be protected from clinical signs of PE on later challenge. Intramuscular vaccination may overcome suggested maternal antibody interference and the need to have a seven day antibiotic-free period in newly weaned pigs. However, in some instances vaccination alone does not appear to prevent PE. In these cases, careful monitoring of the herd will help target antibiotic medication treatments.
Finally a better understanding of the costs and production losses associated with both clinical and sub-clinical PE will help producers target treatments to appropriate periods in pig production. In conclusion, it is a combination of management practices that will enable control of PE with significantly reduced reliance on antibiotic medication.
The author wishes to thank both Australian Pork Limited and the Co-operative Research Centre for High Integrity Pork for their financial support for PE research in Australia over the last twenty years. This research was greatly assisted by colleagues, technical staff and students at the Department of Primary Industries, NSW and the University of Sydney.
Conflicts of Interest
The authors declare no conflict of interest.
- Lawson, G.H.K.; Gebhart, C.J. Proliferative enteropathy. J. Comp. Pathol. 2000, 122, 77–100. [Google Scholar] [CrossRef]
- McOrist, S.; Jasni, S.; Mackie, R.A.; Macintyre, N.; Neef, N.; Lawson, G.H.K. Reproduction of porcine proliferative enteropathy with pure cultures of Ileal symbiont intracellularis. Infect. Immun. 1993, 61, 4286–4292. [Google Scholar]
- Biester, H.E.; Schwarte, L.H. Intestinal adenoma in swine. Am. J. Pathol. 1931, 7, 175–185. [Google Scholar]
- Emsbo, P. Terminal or regional ileitis in swine. Nord. Vet. Med. 1951, 3, 1–28. [Google Scholar]
- Dodd, D.C. Adenomatous intestinal hyperplasia (proliferative ileitis) of swine. Vet. Pathol. 1968, 5, 333–341. [Google Scholar]
- Rowland, A.C.; Rowntree, P.G. A haemorrhagic bowel syndrome associated with intestinal adenomatosis in the pig. Vet. Rec. 1972, 91, 235–241. [Google Scholar]
- O’Hara, P.J. Intestinal haemorrhage syndrome in the pig. Vet. Rec. 1972, 91, 517–518. [Google Scholar]
- Redman Chu, R.M.; Hong, C.B. Haemorrhagic bowel syndrome in pigs in Taiwan. Vet. Rec. 1973, 93, 562–563. [Google Scholar]
- Martinsson, H.; Holmgren, N.; Jonsson, L.; Nordstrom, G. Studies in terminal ileitis in piglets. Sven Vet. 1974, 26, 347–354. [Google Scholar]
- Love, R.J.; Love, D.N.; Edwards, M.J. Proliferative haemorrhagic enteropathy in pigs. Vet. Rec. 1977, 100, 65–68. [Google Scholar]
- Rowland, A.C.; Lawson, G.H.; Maxwell, A. Intestinal adenomatosis in the pig: Occurrence of a bacterium in affected cells. Nature 1973, 243, 417. [Google Scholar] [CrossRef]
- Lawson, G.H.K.; McOrist, S.; Jasni, S.; Mackie, R.A. Intracellular bacteria of porcine proliferative enteropathy: Cultivation and maintenance in vitro. J. Clni. Microbiol. 1993, 31, 1136–1142. [Google Scholar]
- Pointon, A.M. Campylobacter associated intestinal pathology in pigs. Aust. Vet. J. 1989, 66, 90–91. [Google Scholar] [CrossRef]
- Christensen, N.H.; Cullinane, L.C. Monitoring the health of pigs in New Zealand abattoirs. N. Z. Vet. J. 1990, 38, 136–141. [Google Scholar] [CrossRef]
- Holyoake, P.K.; Cutler, R.S.; Caple, I.W. A diagnostic dilemma: Detecting proliferative enteritis in pigs at slaughter. Aust. Vet. J. 1994, 71, 308–309. [Google Scholar] [CrossRef]
- Maes, D.; Verdonck, M.; Castryck, F.; Ducatelle, R.; de Ridder, E.; Weyns, A.; Soenen, K.; de Kruif, A. Porcine proliferative enteropathy: Preliminary epidemiological and pathological observations in Belgian pig herds. Vlaams Diergeneeskundig Tijdschrift 1999, 68, 231–236. [Google Scholar]
- Stege, H.; Jensen, T.K.; Moller, K.; Baekbo, P.; Jorsal, S.E. Prevalence of intestinal pathogens in Danish finishing pig herds. Prev. Vet. Med. 2000, 46, 279–292. [Google Scholar] [CrossRef]
- Roberts, L.; Lawson, G.H.; Rowland, A.C.; Laing, A.H. Porcine intestinal adenomatosis and its detection in a closed pig herd. Vet. Rec. 1979, 104, 366–368. [Google Scholar]
- Holyoake, P.K.; Cutler, R.S.; Caple, I.W. Prevalence of proliferative enteritis on pig farms in Australia. Aust. Vet. J. 1994, 71, 418–422. [Google Scholar] [CrossRef]
- Smith, S.H.; McOrist, S.; Green, L.E. Questionnaire survey of proliferative enteropathy on British pig farms. Vet. Rec. 1998, 142, 690–693. [Google Scholar] [CrossRef]
- Corzo, C.A.; Friendship, R.M.; Dewey, C.E.; Blackwell, T. Seroprevalence of Lawsonia intracellularis in Ontario swine herds. J. Swine Health Prod. 2005, 13, 314–317. [Google Scholar]
- Bane, D.; Norby, B.; Gardner, I.; Roof, M.; Knittel, J.P.; Bush, E.J. Prevalence of Management Risk Factors Associated with Lawsonia intracellularis Seropositivity in the US Swine Herd. In Proceedings of Allen D Leman Swine Conference, University of Minnesota, Minneapolis, MN, USA, 21 Sepetember 1997; pp. 10–11.
- Holyoake, P.K.; Emery, D.; Gonsalves, J.; Donahoo, M.; Collins, A. Prevalence of antibodies to Lawsonia intracellularis in pig herds in Australia. Aust. Vet. J. 2010, 88, 186–188. [Google Scholar] [CrossRef]
- Wilson, T.M.; Chang, K.; Gebhart, C.J.; Kurtz, H.J.; Drake, T.R.; Lintner, V. Porcine proliferative enteritis: Serological, microbiological and pathological studies from three field epizootics. Can. J. Vet. Res. 1986, 50, 217–220. [Google Scholar]
- Winkelman, N.L. Ileitis: An update. Compend. Contin. Educ. Pract. Vet. 1996, 18, S19–S25. [Google Scholar]
- Chang, W.L.; Wu, C.F.; Wu, Y.; Kao, Y.M.; Pan, M.J. Prevalence of Lawsonia intracellularis in swine herds in Taiwan. Vet. Rec. 1997, 141, 103–104. [Google Scholar] [CrossRef]
- Kim, O.; Kim, B.; Chae, C. Prevalence of Lawsonia intracellularis in selected pig herds in Korea as determined by PCR. Vet. Rec. 1998, 143, 587–589. [Google Scholar] [CrossRef]
- Pozo, J.; Carvajal, A.; de Arriba, M.L.; Lanza, I.; Rubio, P. Presence of Lawsonia intracellularis as an Aetiologic Agent in Enteric Disorders in Spanish Swine Herds. In Proceedings of the 15th International Pig Veterinary Society Congress, Birmingham, UK, 5–9 July 1998; p. 416.
- Chiriboga, A.E.; Guimaraes, W.V.; Vanetti, M.C.; Araujo, E.F. Detection of Lawsonia intracellularis in faeces of swine from the main producing regions in Brazil. Can. J. Microbiol. 1999, 45, 230–234. [Google Scholar]
- McOrist, S.; Boid, R.; Lawson, G.H.K. Antigenic analysis of Campylobacter species and an intracellular Campylobacter-like organism associated with porcine proliferative enteropathies. Infect. Immun. 1989, 57, 957–962. [Google Scholar]
- McOrist, S.; Boid, R.; Lawson, G.H.K.; McConnell, I. Monoclonal antibodies to intracellular Campylobacter-like organisms of the porcine proliferative enteropathies. Vet. Rec. 1987, 121, 421–422. [Google Scholar]
- Holyoake, P.K.; Cutler, R.S.; Caple, I.W.; Monckton, R.P. Enzyme-linked immunosorbent assay for measuring ileal symbiont intracellularis-specific immunoglobulin G response in sera of pigs. J. Clin. Microbiol. 1994, 32, 1980–1985. [Google Scholar]
- Knittel, J.P.; Jordan, D.M.; Schwartz, K.J.; Janke, B.H.; Roof, M.B.; McOrist, S.; Harris, D.L. Evaluation of antemortem polymerase chain reaction and serologic methods for detection of Lawsonia intracellularis-exposed pigs. Am. J. Vet. Res. 1998, 59, 722–726. [Google Scholar]
- Guedes, R.M.C.; Gebhart, C.J.; Deen, J.; Winkelman, N.L. Validation of an immunoperoxidase monolayer assay as a serologic test for porcine proliferative enteropathy. J. Vet. Diagn. Investig. 2002, 14, 528–530. [Google Scholar] [CrossRef]
- Boesen, H.T.; Jensen, T.K.; Moler, K.; Nielsen, L.H.; Jungersen, G. Evaluation of a novel enzyme-linked immunosorbent assay for serological diagnosis of porcine proliferative enteropathy. Vet. Microbiol. 2005, 109, 105–112. [Google Scholar] [CrossRef]
- Kroll, J.J.; Eichmeyer, M.A.; Schaeffer, M.L.; McOrist, S.; Harris, D.L.; Roof, M.B. Lipopolysaccharide-based enzyme-linked immunosorbent assay for experimental use in detection of antibodies to Lawsonia intracellularis in pigs. Clin. Diagn. Lab. Immun. 2005, 12, 693–699. [Google Scholar]
- Vu Ngoc, Q.; Nguyen Dang, H.; Cu Huu, P.; Nguyen Ngoc, N.; Collins, A.; Love, R. Epidemiological survey of Lawsonia intracellularis infection and the risk factors in industrial pig farms in Vietnam. Khoa Hoc Ky Thuat Thu Y 2003, 10, 23–31. [Google Scholar]
- Collins, A.M.; Barchia, I.; Gonsalves, J.R.; Fell, S.; Eamens, G.J. Reducing Survival and Transmission of Lawsonia intracellularis in Conventional Piggeries. In Manipulating Pig Production XI; Paterson, J.E., Barker, J.A., Eds.; Australasian Pig Science Association: Werribee, Australia, 2007; p. 125. [Google Scholar]
- Collins, A.M.; Fell, S.A.; Barchia, I.M. Cleaning and disinfection with Virkon S significantly reduces Lawsonia intracellularis survival and transmission to naive pigs. J. Swine Health Prod. 2013, 21, 144–147. [Google Scholar]
- Collins, A.; Gonsalves, J.; Fell, S.; Barchia, I. Comparison of a commercial ELISA with an indirect fluorescent antibody test to detect antibodies to Lawsonia intracellularis in experimentally challenged pigs. Aust. Vet. J. 2012, 90, 97–99. [Google Scholar] [CrossRef]
- Jacobson, M.; Wallgren, P.; Nordengrahn, A.; Merza, M.; Emanuelson, U. Evaluation of a blocking ELISA for the detection of antibodies against Lawsonia intracellularis in pig sera. Acta Vet. Scand. 2011, 53, 23. [Google Scholar] [CrossRef]
- Guedes, R.M.C.; Gebhart, C.J. Onset and duration of fecal shedding, cell-mediated and humoral immune responses in pigs after challenge with a pathogenic isolate or attenuated vaccine strain of Lawsonia intracellularis. Vet. Microbiol. 2003, 91, 135–145. [Google Scholar] [CrossRef]
- Collins, A.M.; Love, R.J. Re-challenge of pigs following recovery from proliferative enteropathy. Vet. Microbiol. 2007, 120, 381–386. [Google Scholar] [CrossRef]
- Guedes, R.M.C.; Gebhart, C.J. Evidence of cell-mediated immune response and specific local mucosal immunoglobulin (Ig) A production against Lawsonia intracellularis in experimentally infected swine. Can. J. Vet. Res. 2010, 74, 97–101. [Google Scholar]
- Cordes, H.; Riber, U.; Jensen, T.K.; Jungersen, G. Cell-mediated and humoral immune responses in pigs following primary and challenge-exposure to Lawsonia intracellularis. Vet. Res. 2012, 43, 9. [Google Scholar] [CrossRef]
- Nogueira, M.G.; Collins, A.M.; Donahoo, M.; Emery, D. Immunological responses to vaccination following experimental Lawsonia intracellularis virulent challenge in pigs. Vet. Microbiol. 2013, 164, 131–138. [Google Scholar] [CrossRef]
- Jones, G.F.; Ward, G.E.; Gebhart, C.J.; Murtaugh, M.P.; Collins, J.E. Use of a DNA probe to detect the intracellular organism of proliferative enteritis in swine feces. Am. J. Vet. Res. 1993, 54, 1585–1590. [Google Scholar]
- Jones, G.F.; Ward, G.E.; Murtaugh, M.P.; Lin, G.; Gebhart, C.J. Enhanced detection of intracellular organism of swine proliferative enteritis, ileal symbiont intracellularis, in feces by polymerase chain reaction. J. Clin. Microbiol. 1993, 31, 2611–2615. [Google Scholar]
- Moller, K.; Jensen, T.K.; Jorsal, S.E.; Leser, T.D.; Carstensen, B. Detection of Lawsoniaintracellularis, Serpulina hyodysenteriae, weakly beta-haemolytic intestinal spirochaetes, Salmonellaenterica, and haemolytic Escherichia coli from swine herds with and without diarrhoea among growing pigs. Vet. Microbiol. 1998, 62, 59–72. [Google Scholar] [CrossRef]
- Collins, A.; Love, R.J.; Pozo, J.; Smith, S.H.; McOrist, S. Studies on the ex vivo survival of Lawsonia intracellularis. Swine Health Prod. 2000, 8, 211–215. [Google Scholar]
- Jacobson, M.; Lofstedt, M.G.; Holmgren, N.; Lundeheim, N.; Fellstrom, C. The prevalences of Brachyspira spp. and Lawsonia intracellularis in Swedish piglet producing herds and wild boar population. J. Vet. Med. 2005, 52, 386–391. [Google Scholar] [CrossRef]
- Echeverria, P.; Taylor, D.; Seriwatana, J.; Brown, J.E.; Lexomboon, U. Examination of colonies and stool blots for detection of enteropathogens by DNA hybridization with eight DNA probes. J. Clin. Microbiol. 1989, 27, 331–334. [Google Scholar]
- Millar, D.S.; Withey, S.J.; Tizard, M.L.V.; Ford, J.G.; Hermon-Taylor, J. Solid-phase hybridization capture of low-abundance target DNA sequences: Application to the polymerase chain reaction detection of Mycobacterium paratuberculosis and Mycobacterium avium subsp. silvaticum. Anal. Biochem. 1995, 226, 325–330. [Google Scholar] [CrossRef]
- La, T.; Collins, A.M.; Phillips, N.D.; Oksa, A.; Hampson, D.J. Development of a multiplex-PCR for rapid detection of the enteric pathogens Lawsonia intracellularis, Brachyspira hyodysenteriae, and Brachyspira pilosicoli in porcine faeces. Lett. Appl. Microbiol. 2006, 42, 284–288. [Google Scholar] [CrossRef]
- Nathues, H.; Oliveira, C.J.B.; Wurm, M.; Beilage, E.G.; Givisiez, P.E.N. Simultaneous detection of Brachyspira hyodysenteriae, Brachyspira pilosicoli and Lawsonia intracellularis in porcine faeces and tissue samples by multiplex-PCR. Zentralblatt Vet. 2007, 54, 532–538. [Google Scholar]
- Nathues, H.; Holthaus, K.; Beilage, E.G. Quantification of Lawsonia intracellularis in porcine faeces by real-time PCR. J. Appl. Microbiol. 2009, 107, 2009–2016. [Google Scholar] [CrossRef]
- Richter, B.; Ladinig, A.; Nedorost, N.; Weissenbock, H. A TaqMan quantitative polymerase chain reaction assay for the detection of Lawsonia intracellularis in fecal and tissue samples from pigs. J. Vet. Diagn. Investig. 2010, 22, 70–73. [Google Scholar] [CrossRef]
- Collins, A.M.; Fell, S.; Pearson, H.; Toribio, J.A. Colonisation and shedding of Lawsonia intracellularis in experimentally inoculated rodents and in wild rodents on pig farms. Vet. Microbiol. 2011, 150, 384–388. [Google Scholar] [CrossRef]
- Stahl, M.; Kokotovic, B.; Hjulsager, C.K.; Breum, S.O.; Angen, O. The use of quantitative PCR for identification and quantification of Brachyspira pilosicoli, Lawsonia intracellularis and Escherichia coli fimbrial types F4 and F18 in pig feces. Vet. Microbiol. 2011, 151, 307–314. [Google Scholar] [CrossRef]
- Pedersen, K.S.; Skrubel, R.; Stege, H.; Angen, O.; Stahl, M.; Hjulsager, C.; Larsen, L.E.; Nielsen, J.P. Association between average daily gain, faecal dry matter content and concentration of Lawsonia intracellularis in faeces. Acta Vet. Scand. 2012, 54, 58. [Google Scholar] [CrossRef]
- Johansen, M.; Nielsen, M.; Dahl, J.; Svensmark, B.; Baekbo, P.; Kristensen, C.S.; Hjulsager, C.K.; Jensen, T.K.; Stahl, M.; Larsen, L.E.; et al. Investigation of the association of growth rate in grower-finishing pigs with the quantification of Lawsonia intracellularis and porcine circovirus type 2. Prev. Vet. Med. 2013, 108, 63–72. [Google Scholar] [CrossRef]
- Collins, A.M.; Barchia, I.M. The critical threshold of Lawsonia intracellularis in pig faeces that causes reduced average daily gains. Vet. Microbiol. 2013. in press. [Google Scholar]
- Pedersen, K.S.; Angen, O.; Nielsen, J.P. Pooling of Faecal Samples for Quantitative Assessment of Lawsonia intracellularis Excretion Levels. In Proceedings of the 22nd International Pig Veterinary Society Congress, Jeju, Korea, 10–13 June 2012; p. 284.
- Collins, A.M.; Barchia, I.M. Optimal Pooling of Faeces to Quantify Lawsonia intracellularis in Clinically and Sub-Clinically Affected Pigs. In Manipulating Pig Production; Pluske, J.A.J., Ed.; Australasian Pig Science Association: Werribee, Australia, 2013; Volume XIV, in press. [Google Scholar]
- Collins, A. Efficacy of Antibiotics to Terminate Faecal Shedding of Lawsonia intracellularis. In Proceedings of the 22nd International Pig Veterinary Society Congress, Jeju, Korea, 10–13 June 2012; p. 675.
- Love, R.J. Gastro Intestinal Disorders of Growers. In Gastroenteric Conditions Seminar; Post-Graduate Committee in Veterinary Science, Ed.; The University of Sydney: Sydney, Australia, 1981; pp. 397–401. [Google Scholar]
- McOrist, S.; Smith, S.H.; Shearn, M.F.H.; Carr, M.M.; Miller, D.J.S. Treatment and prevention of porcine proliferative enteropathy with oral Tiamulin. Vet. Rec. 1996, 139, 615–618. [Google Scholar]
- Schwartz, K.J.; Knittel, J.P.; Walter, D.; Roof, M.; Anderson, M. Effect of oral tiamulin on the development of porcine proliferative enteropathy in a pure-culture challenge model. Swine Health Prod. 1999, 7, 5–11. [Google Scholar]
- Moore, G.M.; Zimmermann, A.G.; Armbruster, G.A. Efficacy of Tylosin Phosphate Administration in Feed for the Prevention and/or Control of Porcine Proliferative Enteropathies (PPE) in Commercial Swine Herds. In Proceedings of the 28th Annual Meeting of the American Association of Swine Practitioners, Quebec City, Canada, 1–4 March 1997; pp. 57–58.
- McOrist, S.; Morgan, J.; Veenhuizen, M.F.; Lawrence, K.; Kroger, H.W. Oral administration of Tylosin phosphate for treatment and prevention of proliferative enteropathy in pigs. Am. J. Vet. Res. 1997, 58, 136–139. [Google Scholar]
- Winkelman, N.L.; Cornell, P.; Bradford, J. Lincomycin Feed Medication and Two Water Medications Against Ileitis Caused by Lawsonia intracellularis. In Proceedings of 29th Annual Meeting of the American Association of Swine Practitioners, Des Moines, IA, USA, 7–10 March 1998; pp. 195–199.
- Winkelman, N.L.; Gebhart, C.; Collins, J.; King, V.; Hannon, M.; Wolff, T. An Evaluation of Aureomycin Chlortetracycline (CTC) Granular Feed Additive for Prevention or Treatment of Swine Ileitis. In Proceedings of the 28th Annual Meeting of the American Association of Swine Practitioners, Quebec City, Canada, 1–4 March 1997; pp. 79–83.
- Pauling, G.E.; Paradis, M.A.; Dick, C.P.; Brennan, J.; Wilson, J. Evaluation of Tylosin Tartrate in Drinking Water for the Treatment of Porcine Proliferative Enteropathy. In Proceedings of the 30th Annual Meeting of the American Association of Swine Practitioners, St. Louis, MO, USA, 28 Feberary–2 March 1999; pp. 119–121.
- Vu, N.Q.; Collins, A.M.; van Dijk, M.; Love, R. Effect of Antibiotic Medication on the Development of Immunity to Lawsonia Intracellularis in Pigs. In Manipulating Pig Production; Cranwell, P.D., Ed.; Australasian Pig Science Association: Werribee, Australia, 2001; p. 148. [Google Scholar]
- Collins, A.M.; van Dijk, M.; Vu Ngoc, Q.; Pozo, J.; Love, R.J. Immunity to Lawsonia intracellularis. In Proceedings of the Allen D Leman Swine Conference, Minneapolis, MN, USA, 16 September 2001; pp. 67–69.
- Love, R.J.; Love, D.N. Control of proliferative haemorrhagic enteropathy in pigs. Vet. Rec. 1977, 100, 473. [Google Scholar]
- Riber, U.; Cordes, H.; Boutrup, T.S.; Jensen, T.K.; Heegaard, P.M.H.; Jungersen, G. Primary infection protects pigs against re-infection with Lawsonia intracellularis in experimental challenge studies. Vet. Microbiol. 2011, 149, 406–414. [Google Scholar] [CrossRef]
- Collins, A.M.; van Dijk, M.; McOrist, S.; Love, R.J. Strategic Medication and Development of Immunity to Lawsonia intracellularis. In Proceedings of the 16th International Pig Veterinary Society Congress, Melbourne, Australia, 17–21 September 2000; Cargill, C., McOrist, S., Eds.; IPVS: Melbourne, Australia, 2000; p. 30. [Google Scholar]
- Flø, H.; Bock, R.; Oppengaard, O.J.; Bergsjø, B.; Lium, B. An Attempt to Eradicate Lawsonia Intracellularis and Brachyspira sp. from Swine Herds. In Proceedings of the 16th International Pig Veterinary Society Congress, Melbourne, Australia, 17–21 Sepetember 2000; p. 66.
- Collins, A. Eradication of Proliferative Enteropathy, Current Limitations in Our Knowledge. In Proceedings of the Australian Pig Veterinarians, Melbourne, Australia, 22–24 October 2006; pp. 47–52.
- Johansen, M.; Baekbo, P.; Jensen, T.K.; Møller, K.; Nielsen, V.R. Attempt to Eradicate Lawsonia intracellularis by Medication in 9 Sow Herds—Preliminary Results. In Proceedings of the 17th International Pig Veterinary Society Congress, Ames, IA, USA, 2–5 June 2002.
- Ellegaard, B.; Koerting, K.; Szancer, J. Attempt to Eradicate Lawsonia intracellularis from an SPF Farm by Medication. In Proceedings of the 20th International Pig Veterinary Society Congress, Durban, South Africa, 22–25 June 2008; p. 152.
- Bane, D.P.; Neumann, E.; Gebhart, C.J.; Gardner, I.A.; Norby, B. Porcine proliferative enteropathy: A case-control study in swine herds in the United States. J. Swine Health Prod. 2001, 9, 155–158. [Google Scholar]
- Bronsvoort, M.; Norby, B.; Bane, D.P.; Gardner, I.A. Management factors associated with seropositivity to Lawsonia intracellularis in US swine herds. J. Swine Health Prod. 2001, 9, 285–290. [Google Scholar]
- Collins, A.M.; Love, R.J. Risk Factors Associated with Lawsonia intracellularis Infection. In Manipulating Pig Production; Paterson, J.E., Ed.; Australasian Pig Science Association: Werribee, Australia, 2003; p. 34. [Google Scholar]
- Holyoake, P.K.; Thomson, P.; Hamilton, D.; Collins, A.M. Ecology of Lawsonia intracellularis infection across rearing systems in Australia. In Proceedings of the 21st International Pig Veterinary Society Congress, Vancouver, Canada, 18–21 July 2010; p. 688.
- Pearce, G.P. Epidemiology of enteric disease in grower and finisher pigs: A postal survey of pig producers in England. Vet. Rec. 1997, 144, 338–342. [Google Scholar] [CrossRef]
- Wattanaphansak, S.; Singer, R.S.; Gebhart, C.J. Evaluation of in vitro bactericidal activity of commercial disinfectants against Lawsonia intracellularis. J. Swine Health Prod. 2010, 18, 11–17. [Google Scholar]
- Kroll, J.J.; Roof, M.; McOrist, S. Evaluation of protective immunity in pigs following oral administration of an avirulent live vaccine of Lawsonia intracellularis. Am. J. Vet. Res. 2004, 65, 559–565. [Google Scholar] [CrossRef]
- Nogueira, M.G.; Collins, A.M.; Emery, D. The Immune Response to a Ten-Times Oral or Intramuscular Immunisation with Lawsonia Vaccine in Weaner Pigs. In Manipulating Pig Production XIII; Australian Pig Science Association: Werribee, Australia, 2011; p. 47. [Google Scholar]
- Holyoake, P.K.; Collins, A.; Donahoo, M.; Lising, R.; Emery, D. Identifying obstacles to reducing the use of antibiotics to control porcine proliferative enteropathy. Aust. Vet. J. 2009, 87, 33–34. [Google Scholar] [CrossRef]
- Lawson, G.H.K.; Rowland, A.C.; Roberts, L.; Fraser, G.; McCartney, E. Proliferative haemorrhagic enteropathy. Res. Vet. Sci. 1979, 27, 46–51. [Google Scholar]
- Charles, S.; Hussain, I.; Choi, C.; Nagaranja, K.; Sivanandan, V. Adjuvanted subunit vaccines for the control of Salmonella enteritidis in turkeys. Am. J. Vet. Res. 1994, 55, 636–642. [Google Scholar]
- Dale, C.J.H.; Strugnell, R.A.; Lew, A.M.; Hasse, D.; Sinistaj, M.; Panaccio, M. Vaccination against Proliferative Enteropathy in Pigs. In Manipulating Pig Production VI; Cranwell, P.D., Ed.; Australasian Pig Science Association: Werribee, Australia, 1997; p. 182. [Google Scholar]
- Jacobs, A.; Vermeij, P.; Sefers, R.; Schrier, C. Vaccine for Protection against Lawsonia intracellularis, Mycoplasma hyopneumoniae and Porcine Circo Virus. US Patent 6,921,536, 26 July 2005. [Google Scholar]
- Paradis, M.A.; Gebhart, C.J.; Toole, D.; Vessie, G.; Winkelman, N.L.; Bauer, S.A.; Wilson, J.B.; McClure, C.A. Sub-clinical ileitis: Diagnostic and perfromance parameters in a multi-dose mucosal homogenate challenge model. J. Swine Health Prod. 2012, 20, 137–141. [Google Scholar]
- Vannucci, F.A.; Foster, D.N.; Gebhart, C.J. Comparative transcriptional analysis of homologous pathogenic and non-pathogenic Lawsonia intracellularis isolates in infected porcine cells. PLoS One 2012, 7, e46708. [Google Scholar] [CrossRef]
- Mapother, M.E.; Joens, L.A.; Glock, R.D. Experimental reproduction of porcine proliferative enteritis. Vet. Rec. 1987, 121, 533–536. [Google Scholar]
- Gogolowski, R.P.; Cook, R.W.; Batterham, E.S. Suboptimal growth associated with porcine intestinal adenomatosis in pigs in nutritional studies. Aust. Vet. J. 1991, 68, 406–408. [Google Scholar] [CrossRef]
- Holyoake, P.K.; Collins, A.; Mullan, B. Simulation of the Economic Impact of Lawsonia intracellularis Infection. In Proceedings of the 21st International Pig Veterinary Society Congress, Vancouver, Canada, 18–21 July 2010; p. 233.
- Holyoake, P.K.; Mullan, B.P.; Cutler, R.S. Simulation of the economic impact of proliferative enteritis on pig production in Australia. Aust. Vet. J. 1996, 73, 89–92. [Google Scholar] [CrossRef]
- McOrist, S.; Smith, S.H.; Green, L.E. Estimate of direct financial losses due to porcine proliferative enteropathy. Vet. Rec. 1997, 140, 579–581. [Google Scholar] [CrossRef]
- Collins, A.M.; Fell, S.; van Straaten, J.; Bolsius, N.; Penrose, L. Increased Variation in Pig Weight and Reduced Feed Intake in Pigs Sub-Clinically Affected with Proliferative Enteropathy. In Proceedings of the 21st International Pig Veterinary Society Congress, Vancouver, Canada, 18–21 July 2010; p. 690.
- Collins, A.M.; Fell, S.; van Straaten, J.; Bolsius, N. Reduced Muscle Growth in Pigs Sub-Clinically Affected with Proliferative Enteropathy. In Proceedings of the 21st International Pig Veterinary Society Congress, Vancouver, Canada, 18–21 July 2010; p. 234.
- Jordan, D.M.; Knittel, J.P.; Schwartz, K.J.; Roof, M.; Hoffman, L.J. A Lawsonia intracellularis transmission study using a pure culture inoculated seeder-pig sentinel model. Vet. Microbiol. 2004, 104, 83–90. [Google Scholar] [CrossRef]
- Jordan, D.M.; Knittel, J.P.; Schmoll, E.M.; Schwartz, K.J.; Roof, M.B.; Larson, D.J.; Hoffman, L.J. A Lawsonia intracellularis Transmission Study Using a Pure Culture Inoculated Seeder-Pig Sentinel Model. In Proceedings of the American Association of Swine Practitioners, Quebec, Canada, 1–4 March 1997; pp. 243–248.
- Jones, G.F.; Ward, G.E.; Murtaugh, M.P.; Rose, R.; Gebhart, C.J. Relationship between Ileal symbiont intracellularis and porcine proliferative enteritis. Infect. Immun. 1993, 61, 5237–5244. [Google Scholar]
- Smith, S.H.; McOrist, S. Development of persistent intestinal infection and excretion of Lawsonia intracellularis by piglets. Res. Vet. Science 1997, 62, 6–10. [Google Scholar] [CrossRef]
- Stabel, T.J.; Fedorka-Cray, P.J. Effect of 2-deoxy-d-glucose induced stress on Salmonella choleraesuis shedding and persistence in swine. Res. Vet. Sci. 2004, 76, 187–194. [Google Scholar] [CrossRef]
- Jensen, T.K.; Møller, K.; Lindecrona, R.; Jorsal, S.E. Detection of Lawsonia intracellularis in the tonsils of pigs with proliferative enteropathy. Res. Vet. Sci. 2000, 68, 23–26. [Google Scholar] [CrossRef]
- Collins, A.M.; Fell, S.; Gonsalves, J.; Barchia, I. Immune Suppression does not Lead to a Carrier State in Pigs Previously. Infected with Lawsonia intracellularis. In Manipulating Pig Production XII; Barneveld, R.J., Ed.; Australasian Pig Science Association: Werribee, Australia, 2009; p. 42. [Google Scholar]
- Starek, M.; Bilkei, G. Sows seropositive to Lawsonia intracellularis (LI) influence performance and seropositivity of their offspring. ACTA Vet. Brno 2004, 73, 341–345. [Google Scholar] [CrossRef]
- Jensen, T.K.; Vigre, H.; Sorensen, V.; Moller, K. Naturally acquired Lawsonia intracellularis infection in pigs studied from weaning to slaughter by indirect immunofluorescence antibody test and polymerase chain reaction on faeces. Res. Vet. Sci. 2005, 79, 93–98. [Google Scholar] [CrossRef]
- Farmer, C.; Sorensen, M.T. Factors affecting mammary development in gilts. Livest. Prod. Sci. 2001, 70, 141–148. [Google Scholar] [CrossRef]
- Rooke, J.A.; Bland, I.M. The acquisition of passive immunity in the new-born piglet. Livest. Prod. Sci. 2002, 78, 13–23. [Google Scholar] [CrossRef]
- Bandrick, M.; Pieters, M.; Pijoan, C.; Molitor, T.W. Passive transfer of maternal Mycoplasma hyopneumoniae—Specific cellular immunity to piglets. Clin. Vaccine Immunol. 2008, 15, 540–543. [Google Scholar] [CrossRef]
- Pozo, J.; Collins, A.M.; Rubio, P.; Love, R.J. Maternal Immunity to Lawsonia intracellularis Infection. In Proceedings of the 16th International Pig Veterinary Society Congress, Melbourne, Australia, 17–21 Sepetember 2000; Cargill, C., McOrist, S., Eds.; Causal Productions Pty Limited: Melbourne, Australia, 2000; p. 108. [Google Scholar]
- Chang, K.; Kurtz, H.J.; Ward, G.E.; Gebhart, C.J. The Use of Weaned Mouse as a Model to Study the Role of Campylobacter Species in Proliferative Enteritis of Swine. In Campylobacter III: The Third International Workshop on Campylobacter Infections; Pearson, A.D., Ed.; Public Health Laboratory Service: Ottawa, Canada, 1985; p. 102. [Google Scholar]
- Vandenberghe, J.; Verheyen, A.; Lauwers, S.; Geboes, K. Spontaneous adenocarcinoma of the ascending colon in Wistar rats: The intracytoplasmic presence of a Campylobacter-like bacterium. J. Comp. Pathol. 1985, 95, 45–55. [Google Scholar] [CrossRef]
- Eriksen, K.; Landsverk, T.; Bratberg, B. Morphology and immunoperoxidase studies of intestinal adenomatosis in the blue fox, Alopex lagopus. J. Comp. Pathol. 1990, 102, 265–278. [Google Scholar] [CrossRef]
- Hotchkiss, C.E.; Shames, B.; Perkins, S.E.; Fox, J.G. Proliferative enteropathy of rabbits—The intracellular Campylobacter-like organism is closely related to Lawsonia intracellularis. Lab. Anim. Sci. 1996, 46, 623–627. [Google Scholar]
- Williams, N.M.; Harrison, L.R.; Gebhart, C.J. Proliferative enteropathy in a foal caused by Lawsonia intracellularis-like bacterium. J. Vet. Diagn. Investig. 1996, 8, 254–256. [Google Scholar] [CrossRef]
- Cooper, D.M.; Swanson, D.L.; Gebhart, C.J. Diagnosis of proliferative enteritis in frozen and formalin-fixed, paraffin-embedded tissues from a hamster, horse, deer and ostrich using a Lawsonia intracellularis-specific multiplex PCR assay. Vet. Microbiol. 1997, 54, 47–62. [Google Scholar] [CrossRef]
- LeMarchand, T.X.; Tully, T.N.J.; Shane, S.M.; Duncan, D.E. Intracellular Campylobacter-like organisms associated with rectal prolapse and proliferative enteroproctitis in emus (Dromaius novaehollandiae). Vet. Pathol. 1997, 34, 152–156. [Google Scholar] [CrossRef]
- Tomanova, K.; Bartak, P.; Smola, J. Detection of Lawsonia intracellularis in wild pigs in the Czech Republic. Vet. Rec. 2002, 151, 765–767. [Google Scholar]
- McOrist, S.; Blunt, R.; Gebhart, C.J. Pig-associated Lawsonia intracellularis in various on-farm dipterous fly stages. J. Swine Health Prod. 2011, 19, 277–283. [Google Scholar]
- Collins, A.M.; Love, R.J.; Jasni, S.; McOrist, S. Attempted infection of mice, rats and chickens by porcine strains of Lawsonia intracellularis. Aust. Vet. J. 1999, 77, 120–122. [Google Scholar] [CrossRef]
- Go, Y.Y.; Lee, J.K.; Ye, J.Y.; Lee, J.B.; Park, S.Y.; Song, C.S.; Kim, S.K.; Choi, I.S. Experimental reproduction of proliferative enteropathy and the role of IFN-gramma in protective immunity against Lawsonia intracellularis in mice. J. Vet. Sci. 2005, 6, 357–359. [Google Scholar]
- Murakata, K.; Sato, A.; Yoshiya, M.; Kim, S.; Watarai, M.; Omata, Y.; Furuoka, H. Infection of different strains of mice with Lawsonia intracellularis derived from rabbit or porcine proliferative enteropathy. J. Comp. Pathol. 2008, 139, 8–15. [Google Scholar] [CrossRef]
- Friedman, M.; Bednar, V.; Klimes, J.; Smola, J.; Mrlik, V. Lawsonia intracellularis in rodents from pig farms with the occurrence of porcine proliferative enteropathy. Lett. Appl. Microbiol. 2008, 47, 117–121. [Google Scholar] [CrossRef]
- De Viott, A.M.; Franca, S.A.; Vannucci, F.A.; Cruz Junior, E.C.C.; Costa, M.C.; Gebhart, C.J.; Guedes, R.M.C. Infection of sparrows (Passer domesticus) and different mice strains with Lawsonia intracellularis. Pesqui. Vet. Bras. 2013, 33, 372–378. [Google Scholar] [CrossRef]
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