Next Article in Journal
Correction: Jacobo-Velázquez, D.A and Cisneros-Zevallos, L. An Alternative Use of Horticultural Crops: Stressed Plants as Biofactories of Bioactive Phenolic Compounds. Agriculture 2012, 2, 259-271
Next Article in Special Issue
Risk Priority Number: A Measuring Instrument for Hygienic Management on Broiler Farms, Reflecting Their Campylobacter Status
Previous Article in Journal
Silencing of Mg-pat-10 and Mg-unc-87 in the Plant Parasitic Nematode Meloidogyne graminicola Using siRNAs
Previous Article in Special Issue
Integration of Epidemiological Evidence in a Decision Support Model for the Control of Campylobacter in Poultry Production
Agriculture 2013, 3(3), 579-595; doi:10.3390/agriculture3030579

Identification of Multiple Subtypes of Campylobacter jejuni in Chicken Meat and the Impact on Source Attribution

1,* , 1
1,†† and 1
1 Institute of Environmental Science and Research Limited, Christchurch Science Centre, P.O. Box 29-181, Christchurch 8041, New Zealand 2 University of Canterbury, Private Bag 4800, Christchurch 8041, New Zealand Current address: Centers for Disease Control and Prevention-Emerging Infectious Diseases, Beijing 100000, China †† Current address: Affordable Water Limited, 72 Argyle Street, Herne Bay, Auckland 1011, New Zealand
* Author to whom correspondence should be addressed.
Received: 17 June 2013 / Revised: 15 August 2013 / Accepted: 29 August 2013 / Published: 18 September 2013
(This article belongs to the Special Issue Food Safety Management and Poultry Production)
View Full-Text   |   Download PDF [692 KB, 23 September 2013; original version 18 September 2013]   |   Browse Figures


Most source attribution studies for Campylobacter use subtyping data based on single isolates from foods and environmental sources in an attempt to draw epidemiological inferences. It has been suggested that subtyping only one Campylobacter isolate per chicken carcass incurs a risk of failing to recognise the presence of clinically relevant, but numerically infrequent, subtypes. To investigate this, between 21 and 25 Campylobacter jejuni isolates from each of ten retail chicken carcasses were subtyped by pulsed-field gel electrophoresis (PFGE) using the two restriction enzymes SmaI and KpnI. Among the 227 isolates, thirteen subtypes were identified, the most frequently occurring subtype being isolated from three carcasses. Six carcasses carried a single subtype, three carcasses carried two subtypes each and one carcass carried three subtypes. Some subtypes carried by an individual carcass were shown to be potentially clonally related. Comparison of C. jejuni subtypes from chickens with isolate subtypes from human clinical cases (n = 1248) revealed seven of the thirteen chicken subtypes were indistinguishable from human cases. None of the numerically minor chicken subtypes were identified in the human data. Therefore, typing only one Campylobacter isolate from individual chicken carcasses may be adequate to inform Campylobacter source attribution.
Keywords: multiple subtypes; chicken carcass; diversity; enrichment; C. jejuni multiple subtypes; chicken carcass; diversity; enrichment; C. jejuni
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Devane, M.L.; Gilpin, B.J.; Robson, B.; Klena, J.D.; Savill, M.G.; Hudson, J.A. Identification of Multiple Subtypes of Campylobacter jejuni in Chicken Meat and the Impact on Source Attribution. Agriculture 2013, 3, 579-595.

View more citation formats

Related Articles

Article Metrics


[Return to top]
Agriculture EISSN 2077-0472 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert