Optimization of Polymer-ECM Composite Scaffolds for Tissue Engineering: Effect of Cells and Culture Conditions on Polymeric Nanofiber Mats
Abstract
:1. Introduction
2. Results and Discussion
2.1. Fabrication of Fibrous Scaffolds
2.2. Stability of the Fibrous Scaffolds
2.3. Deposition of ECM on Scaffolds Cultured in the Presence of Cells
2.4. Change in Scaffold Stiffness as Function of Time
2.5. Testing the Full Cellularization-Decell-Recell Cycle of ECM-Polymer Composite Scaffolds
3. Materials and Methods
3.1. Fabrication of Fibrous Scaffolds
3.2. Electrospinning
3.3. Fiber Mat Characterization
3.4. Cell Culture
3.5. Decellularization
3.6. Cell Culture on ECM-Polymer Composite Scaffolds
3.7. Immunostaining
3.8. Statistical Analysis
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Goyal, R.; Guvendiren, M.; Freeman, O.; Mao, Y.; Kohn, J. Optimization of Polymer-ECM Composite Scaffolds for Tissue Engineering: Effect of Cells and Culture Conditions on Polymeric Nanofiber Mats. J. Funct. Biomater. 2017, 8, 1. https://doi.org/10.3390/jfb8010001
Goyal R, Guvendiren M, Freeman O, Mao Y, Kohn J. Optimization of Polymer-ECM Composite Scaffolds for Tissue Engineering: Effect of Cells and Culture Conditions on Polymeric Nanofiber Mats. Journal of Functional Biomaterials. 2017; 8(1):1. https://doi.org/10.3390/jfb8010001
Chicago/Turabian StyleGoyal, Ritu, Murat Guvendiren, Onyi Freeman, Yong Mao, and Joachim Kohn. 2017. "Optimization of Polymer-ECM Composite Scaffolds for Tissue Engineering: Effect of Cells and Culture Conditions on Polymeric Nanofiber Mats" Journal of Functional Biomaterials 8, no. 1: 1. https://doi.org/10.3390/jfb8010001