Next Article in Journal
Biomimetic Strategies for Sensing Biological Species
Next Article in Special Issue
Fast and Sensitive Interferon-γ Assay Using Supercritical Angle Fluorescence
Previous Article in Journal
Quinone-Based Polymers for Label-Free and Reagentless Electrochemical Immunosensors: Application to Proteins, Antibodies and Pesticides Detection
Previous Article in Special Issue
Evaluating Inhibition of the Epidermal Growth Factor (EGF)-Induced Response of Mutant MCF10A Cells with an Acoustic Sensor
Biosensors 2013, 3(1), 77-88; doi:10.3390/bios3010077

Single Step Nanoplasmonic Immunoassay for the Measurement of Protein Biomarkers

1 Department of Ophthalmology, University of Miami-Bascom Palmer Eye Institute, Miami, FL 33136, USA 2 Department of Structural Biology, Stanford University, Palo Alto, CA 94305, USA
* Author to whom correspondence should be addressed.
Received: 11 December 2012 / Revised: 18 January 2013 / Accepted: 1 February 2013 / Published: 6 February 2013
(This article belongs to the Special Issue Immunosensors 2012)
View Full-Text   |   Download PDF [377 KB, 7 February 2013; original version 6 February 2013]   |   Browse Figures


A nanoplasmonic biosensor for highly-sensitive, single-step detection of protein biomarkers is presented. The principle is based on the utilization of the optical scattering properties of gold nanorods (GNRs) conjugated to bio-recognition molecules. The nanoplasmonic properties of the GNRs were utilized to detect proteins using near-infrared light interferometry. We show that the antibody-conjugated GNRs can specifically bind to our model analyte, Glucose Transporter-1 (Glut-1). The signal intensity of back-scattered light from the GNRs bound after incubation, correlated well to the Glut-1 concentration as per the calibration curve. The detection range using this nanoplasmonic immunoassay ranges from 10 ng/mL to 1 ug/mL for Glut-1. The minimal detectable concentration based on the lowest discernable concentration from zero is 10 ng/mL. This nanoplasmonic immunoassay can act as a simple, selective, sensitive strategy for effective disease diagnosis. It offers advantages such as wide detection range, increased speed of analysis (due to fewer incubation/washing steps), and no label development as compared to traditional immunoassay techniques. Our future goal is to incorporate this detection strategy onto a microfluidic platform to be used as a point-of-care diagnostic tool.
Keywords: gold nanorods; optical coherence tomography; protein biomarkers; immunoassay; surface plasmon resonance; glucose transporter-17  gold nanorods; optical coherence tomography; protein biomarkers; immunoassay; surface plasmon resonance; glucose transporter-17 ;
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Prabhulkar, S.; de la Zerda, A.; Paranjape, A.; Awdeh, R.M. Single Step Nanoplasmonic Immunoassay for the Measurement of Protein Biomarkers. Biosensors 2013, 3, 77-88.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Biosensors EISSN 2079-6374 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert