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Review

Breaking Barriers to an AIDS Model with Macaque-Tropic HIV-1 Derivatives

by
Rajesh Thippeshappa
,
Hongmei Ruan
and
Jason T. Kimata
*
Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, TX 77030, USA
*
Author to whom correspondence should be addressed.
Biology 2012, 1(2), 134-164; https://doi.org/10.3390/biology1020134
Submission received: 16 May 2012 / Revised: 14 June 2012 / Accepted: 18 June 2012 / Published: 5 July 2012
(This article belongs to the Special Issue Structural and Molecular Biology of HIV)
Versions Notes

Abstract

:
The development of an animal model of human immunodeficiency virus type 1 (HIV-1)/AIDS that is suitable for preclinical testing of antiretroviral therapy, vaccines, curative strategies, and studies of pathogenesis has been hampered by the human-specific tropism of HIV-1. Although simian immunodeficiency virus (SIV) or HIV-1/SIV chimeric viruses (SHIVs)-rhesus macaque models are excellent surrogates for AIDS research, the genetic differences between SIV or SHIV and HIV-1 limit their utility as model systems. The identification of innate retroviral restriction factors has increased our understanding about blockades to HIV-1 replication in macaques and provided a guide for the construction of macaque-tropic HIV-1 clones. However, while these viruses replicate in macaque cells in vitro, they are easily controlled and have not caused AIDS in host animals, indicating that we may not fully understand the restrictive barriers of innate immunity. In this review, we discuss recent findings regarding HIV-1 restriction factors, particularly as they apply to cross-species transmission of primate lentiviruses and the development of a macaque model of HIV-1/AIDS.

1. Introduction

A significant problem for testing novel antiretroviral drugs and vaccines against human immunodeficiency virus type 1 (HIV-1) has been the lack of an animal model due to the inability of the virus to replicate in species other than humans. Although chimpanzees and gibbon apes can be experimentally infected with HIV-1, AIDS-like disease is not typically observed. A recent report suggests that simian immunodeficiency virus cpz (SIVcpz), the precursor of HIV-1, may cause AIDS in wild chimpanzees [1]. However, it is not clear how commonly this happens. Moreover, the endangered status and the high maintenance cost associated with apes make them unsuitable model hosts for experimental studies of HIV-1 [2]. Other nonhuman primate species, including Asian macaques that are used for the SIVmac AIDS model, have not proven to be susceptible to HIV-1. Thus, this narrow host range of HIV-1 has complicated the development of a nonhuman primate model of HIV-1 infection and disease.
In the past several years, studies have identified anti-retroviral factors, collectively called intrinsic immunity, in human and nonhuman primate cells that restrict infection. Accessory proteins expressed by HIV-1 (i.e., viral infectivity factor (Vif), viral protein U (Vpu), viral protein R (Vpr) and negative factor (Nef)) have been shown to play key roles in overcoming intrinsic immunity in human cells. Thus, successful transmission of HIV-1 to nonhuman primates may depend on whether these accessory proteins function in model host species. Here, we review the literature about innate barriers to HIV-1 infection and application of this knowledge to developing a macaque model of HIV-1/AIDS.

2. Cross-Species Transmission of HIV-1

The emergence of HIV-1 is believed to have started with the transmission of SIVcpz from chimpanzees (Pan troglodytes) to humans [3]. SIVcpz entered the human population on at least three independent occasions, with each transmission event giving rise to HIV-1 groups M, N, and O [4]. Recently, a new HIV-1 that is closely related to an SIV that infects gorillas (Gorilla gorilla gorilla) (SIVgor) has also been identified. This virus appears to represents a fourth transmission lineage (HIV-1 group P) [5]. Interestingly, SIVcpz is believed to have evolved from successful infection and recombination of two SIV strains, SIVgsn from greater spot-nosed monkeys (Cercopithecus nictitans) and SIVrcm from red capped mangabeys (Cercocebus torquatus), in chimpanzees [6]. During recombination, SIVcpz acquired accessory genes such as vpu from SIVgsn and nef from SIVrcm. A recent report suggests that host dependent adaptation of vpu and nef may have enhanced replication of SIVcpz in chimpanzees [7]. Other recent studies provide additional insights on adaptive mutations in SIVcpz genes that may have enabled it to jump from chimpanzees to humans [7,8,9,10]. Thus, these evolutionary studies on the origin of HIV-1 suggest that primate lentiviruses may be highly dependent on the functional activity of accessory genes for jumping into new species.

3. SIV and SHIV Macaque Models

Nonhuman primate models have been developed to study AIDS pathogenesis and to evaluate the efficacy of vaccines and drugs. The earliest animal model developed was infection of rhesus macaques (RM, Macaca mulatta) with a simian immunodeficiency virus. Many African monkey species harbor SIVs and live with high virus loads without developing disease [11,12,13,14,15,16,17]. However, SIV isolated from sooty mangabeys (SM, Cercocebus atys) can cause AIDS when inoculated into RMs [18,19]. Infectious molecular clones of SIVsm, named SIVmac, were generated from the infected RMs and shown to cause AIDS in naïve RMs [20,21]. SIVmac has also been shown to cause AIDS-like disease in cynomolgus monkeys (CM, Macaca fascicularis) and pig-tailed macaques (PTM, Macaca nemestrina) [22,23]. Consequently, SIV infection of macaques is widely used as a model for AIDS pathogenesis. This SIV model has also helped provide an understanding of the importance of viral genes, such as vpx, vpr and nef in SIV replication in vivo [24,25,26,27,28,29,30].
Although the SIV-macaque model has been informative for studies of pathogenesis, it does have some shortcomings (reviewed in Ambrose et al. [31]). A primary concern is the genetic differences between HIV-1 and SIVmac. For example, HIV-1 encodes vpu, which makes it difficult to study its role in pathogenicity since a homolog does not exist in SIVmac. Additionally, the vpx gene is present in SIVmac but not HIV-1. There are also differences in the reverse transcriptase (RT) and protease (Pr) enzymes [32,33], which make it difficult to evaluate the efficacy of drugs targeting these enzymes when using SIVmac as the model virus. For example, non-nucleoside RT inhibitors block the HIV-1 RT but not SIV RT [34]. Finally, the differences in sequences of HIV-1 and SIV may affect humoral and cellular immune responses. First, the viruses are not likely to share immunodominant cytotoxic T-cell (CTL) epitopes. Second, structural differences in the HIV-1 and SIV Env proteins may lead to qualitative differences in the antibody responses [35,36]. Moreover, neutralizing antibodies to the SIV Env are not crossreactive with the HIV-1 Env [37]. Additionally, there could be qualitative differences in the immune response to HIV-1 compared to SIV in nonhuman primates. Together, these differences limit the utility of the SIV-macaque model for translational studies of antiviral therapy or vaccines.
To overcome some of the limitations of the SIV-macaque model for vaccine and antiretroviral inhibitor studies, chimeric viruses that incorporate parts of the HIV-1 genome into the background of SIV have been generated. Shibata et al. constructed initial SIV and HIV-1 chimeric viruses (SHIVs) by inserting HIV-1 genes such as tat, rev, vpu and env into SIVmac239 backbone [36]. Subsequently, several independent groups showed that novel SHIVs could replicate and cause disease after passing the viruses through macaques [38,39,40]. This model has helped increase our understanding of the role of envelope in pathogenesis [41,42,43]. It has also been useful for determining the efficacy of passively transferred neutralizing antibodies [44,45,46,47], vaccines [48,49], and microbicides [50] in preventing SHIV infection. To study the efficacy of non-nucleoside inhibitors of reverse transcriptase (RT), chimeric viruses in which the RT of SIV has been replaced with that from HIV-1 (RT-SHIV) have also been developed [34,51,52,53,54,55,56]. While these SHIVs have improved utility, the remaining issue is that these viruses mainly consist of SIV sequences and single HIV-1 genes. Thus, it is not possible to test vaccines or antiretroviral drugs targeting multiple HIV-1 proteins. On the other hand, while there are shortcomings to the SHIV-macaque model for translational studies, the construction of pathogenic SHIV chimeras revealed which HIV-1 genes are functional in macaque hosts, narrowing the determinants that may be critical for cross-species transmission of HIV-1 into macaques.

4. Innate Cellular Restriction Factors

4.1. Overview

In the last decade, cellular factors have been identified that inhibit retroviral replication at different stages of the life cycle. Although HIV-1 and SIV can overcome these restriction factors in their natural hosts, they may not do so in other species, suggesting that innate restriction factors act as barriers for cross-species transmission of HIV-1 and SIV. The four major restriction factors that have been identified are the apolipoprotein B mRNA editing enzyme catalytic polypeptide 3 (APOBEC3 or A3) family of proteins, tripartite motif containing (TRIM) family of proteins, BST2/CD317/Tetherin, and sterile alpha motif (SAM) and histidine/aspartic acid (HD) domain containing protein 1 (SAMHD1) (Table 1). Interestingly, each is upregulated by type 1 interferons, linking restriction to the innate immune response. Additionally, accumulating evidence indicates that each gene has been under strong positive selection in nonhuman primates and humans in response to lentiviral infections, further indicating their importance for controlling infection [57,58,59,60,61,62,63].

4.2. APOBEC3 Proteins

The APOBEC3 family members are cytidine deaminases. This family is comprised of seven members in humans: A3A, A3B, A3C, A3CD, A3F, A3G, and A3H [64]. The antiviral activity of APOBEC3 family proteins was first identified by Sheehy et al. , who discovered A3G as a factor that renders Vif deficient HIV-1 progeny virions non-infectious in non-permissive cells but not permissive cells [65]. In the absence of Vif, A3G is packaged into HIV-1 progeny virions [66]. This requires an interaction of A3G with the nucleocapsid region of the Gag protein and viral or cellular RNAs (reviewed in [67]). Only a few incorporated A3G molecules are enough to exhibit antiviral activity [68]. When the progeny virions infect the next target cell, the incorporated A3G deaminates cytidine (C) to uracil (U) in the minus strand DNA during reverse transcription. Uracil then codes for adenine during the synthesis of positive strand DNA, resulting in accumulation of deleterious G to A mutations in the viral DNA [69,70,71,72]. G to A mutated genomes either undergo degradation [73] or lead to expression and degradation of truncated or misfolded proteins. Processed peptides may enhance recognition of infected cells by CD8+ cytotoxic T lymphocytes [74]. Additional family members of APOBEC3, such as A3F, A3C, and A3D have also been shown to affect the replication of HIV-1 [75,76,77,78,79]. Finally, A3G has also been shown to affect HIV-1 replication in a cytidine deaminase independent mechanism by inhibiting reverse transcription of viral RNA [80,81,82].
Vif inhibits the antiviral activity of APOBEC3 family proteins by preventing their incorporation into progeny virions. Several reports have shown that Vif interacts with A3G and facilitates its degradation by directing it to the proteasome [66,83,84,85,86,87]. Proteosome mediated degradation of A3G requires an interaction of Vif with ElonginB-ElonginC-Cullin5 E3 ligase complex. This Vif-E3 ligase complex then ubiquitinates A3G, which undergoes proteasome mediated degradation. Some reports indicate that Vif may also downregulate A3G expression [88,89].
Table
Table 1. Restriction factors and the counter measures.
Table 1. Restriction factors and the counter measures.
Restriction FactorsMechanism of InhibitionCounter Measures Used by LentivirusesSpecies–Specific Counter Measures
Restriction FactorsInhibited by:Resistant to:
ABOBEC3 familyIntroduce G to A mutations, reduce infectivity, may affect reverse transcriptionVif prevents encapsidation of APOBEC3 family proteins in to the progeny virionsRM, AGM A3GSIVmacVif, SIVagmVifHIV-1 Vif
CPZ, hA3GHIV-1 VifSIVagmVif
BST2Restrict release of mature progeny virions from the cell surfaceHIV-1 Vpu, SIV Nef, and HIV-2 Env overcome BST2 function either by degrading or downmodulation of cell surface expressionCPZ, hBST2HIV-1 VpuSIVmacNef
RM, PTM BST2SIVmacNefHIV-1 Vpu
SAMHD1Reduces dNTP pool required for cDNA synthesisVpx leads to proteasome mediated degradation of SAMHD1Human, gibbon, RM SAMHD1Vpx from HIV-2rod, SIVmac, and SIVsmHIV-1 Vpr
Restriction FactorsMechanism of ActionEscape MechanismTRIM ProteinsResistant VirusesInhibited Viruses
TRIM familyBind to incoming viral capsid and block infection at or before reverse transcriptionViral capsid mutations confer resistance to TRIM5 family proteins in their natural hostRM, CM TRIM5αSIVmacHIV-1
Human TRIM5αHIV-1N-MLV, SIVmac
AGM TRIM5αSIVagmHIV-1, HIV-2, SIVmac
OWM TRIMcypSIVmacHIV-1
RM TRIMcypHIV-1HIV-2, SIVmac
PTM TRIMcypHIV-1FIV, SIVagm, HIV-2
Several studies have shown that Vif counteraction of A3G occurs in a species-specific manner [90,91,92,93,94]. For example, RM and African green monkey (AGM, Chlorocebus aethiops) A3G are not recognized by the HIV-1 Vif protein and therefore block HIV-1 infection; by contrast, they are inhibited by both the SIVmac and SIVagmVif proteins [90]. However, human A3G (hA3G) and chimpanzee A3G (CPZ A3G) are inhibited by the HIV-1 Vif protein but are resistant to Vif of SIVagm [90]. This species-specific activity of Vif likely limits transmission of lentiviruses to humans, apes, and other nonhuman primates. Interestingly, SIVcpz and SIVsm can overcome human A3G antiviral activity [8], suggesting a possible role for Vif mediated counteraction of A3G in cross-species transmission of SIVcpz and SIVsm into the human population and emergence of HIV-1 and HIV-2. In 2004, four independent groups showed that the amino acid at position 128 in A3G (D in hA3G and K in AGM A3G) confers species specificity [91,92,93,94]. Mutation of D to K in hA3G rendered it sensitive to inhibition by the SIV Vif protein and resistant to HIV-1 Vif. On the other hand, changing K to D in AGM A3G made it sensitive to the HIV-1 Vif protein but resistant to the SIV Vif protein [91,92,93,94]. Amino acids 14 to 17 (DRMR) in the HIV-1 Vif and SERQ or SEMQ found in SIVagmVif likely play a role in mediating the interaction with the amino acid at position 128 in both hA3G and AGM A3G, respectively. Substituting DRMR in the HIV-1 Vif with SERQ or SEMQ, enables it to degrade both the AGM and RM A3G proteins but abolishes the interaction with hA3G. Similarly, changing the SERQ residues to DRMR in SIVagmVif redirects targeted degradation from AGM A3G to hA3G. The species-specific activity of HIV-1 Vif is likely governed by the interaction of positive residues in DRMR with the negatively charged D128 in hA3G [95]. Overall, these studies support a hypothesis that Vif-mediated inhibition of macaque APOBEC3 family proteins could be important for cross-species transmission of HIV-1 to macaques.

4.3. TRIM5α and TRIMcyp

HIV-1 does not infect Old World macaques such as RM and CMs due to a post-entry block that occurs before reverse transcription [96,97]. This post-entry block was initially named lentivirus restriction factor 1 or Lv1. AGM cells also exhibit a similar block to lentiviruses, including HIV-1, HIV-2, EIAV, and SIVmac [98,99]. In 2004, Stremlau et al. identified TRIM5α as the inhibitory factor by screening a rhesus macaque complementary DNA (cDNA) expression library for genes that would block HIV-1 infection in human cells [100]. TRIM5α belongs to the tripartite motif (TRIM) family of proteins that contain: (i) a RING finger, which presumably has E3 ubiquitin ligase activity; (ii) a B-box2 domain, which plays a role in higher order self-association of TRIM5α oligomers; and (iii) a coiled-coil domain, which is required for dimerization of TRIM5α. Interestingly, TRIM5α also contains a C-terminal B30. 2/SPRY domain that plays a role in the detection of incoming viral capsid proteins [101]. Following recognition of the viral capsid, TRIM5α links the viral core to an ubiquitin-proteasome-dependent pathway. This results in disruption of the preintegration complex and suppression of reverse transcription [101]. Several reports also suggest a proteasome independent pathway of HIV-1 restriction by TRIM5α. If the proteasome pathway is inhibited, HIV-1 infection proceeds through reverse transcription; however, nuclear entry of viral DNA is impaired [102,103].
New World monkey cells, such as those of Owl monkeys (OWM, Aotus trivirgatus), also exhibit a post-entry barrier to HIV-1 infection [104]. Interestingly, a novel TRIM5-cyclophilin A fusion protein (TRIMcyp) was identified as the inhibitory molecule preventing HIV-1 infection in this species [105,106]. The TRIMcyp fusion protein arose with LINE-1 mediated retrotransposition of the cyclophilinA cDNA into the TRIM5 locus [105]. The cyclophilinA domain in the TRIMcyp protein functions like the B30. 2 domain of TRIM5α and binds to the incoming HIV-1 capsid. Interestingly, cyclophilin A, which interacts with the proline rich loop on the surface of the HIV-1 capsid, has been shown to enhance HIV-1 replication in human cells [107,108]. However, cyclophilin A in Old World monkey cells blocks HIV-1 infection. Two reports suggest that cyclophilinA renders HIV-1 sensitive to the antiviral action of TRIM5α in Old World macaques, although the mechanism of action is not entirely clear [109,110].
Both TRIM5α and TRIMcyp proteins exhibit species-specific restriction. This species specificity is determined by the sequence variations in the B30. 2/SPRY or cyclophilinA domain in different monkey species and amino acid differences in the viral capsid [111,112,113]. Both RM TRIM5α and CM TRIM5α restrict HIV-1 but not SIVmac; whereas human TRIM5α is less effective against HIV-1 and SIVmac, but potently restricts N-tropic murine leukemia virus (N-MLV) [100,111,114]. AGM TRIM5α shows restriction activity against HIV-1, HIV-2, and SIVmac, but it fails to inhibit SIVagm [111,115,116]. In the case of TRIMcyp proteins, OWM TRIMcyp interferes with HIV-1 but not SIVmac [105,106], while the RM TRIMcyp restricts HIV-2 and SIVagm but does not inhibit either HIV-1 or SIVmac [112,117]. Interestingly, neither the PTM nor the CM TRIMcyp blocks either HIV-1 or SIVmac239 infection [118]. However, PTM TRIMcyp restricts FIV, HIV-2 and SIVagm infection [112]. These studies suggest that both the TRIM5α and TRIMcyp proteins may also contribute to controlling cross-species transmission of lentiviruses.

4.4. BST2/Tetherin/CD317

Bone marrow stromal antigen 2 (BST2), also known as tetherin, CD317, or HM1.24 antigen, is a type II membrane protein with an amino-terminal cytoplasmic tail, a single transmembrane domain, an extracellular coiled-coil domain, and a GPI membrane anchor at the C-terminus [119]. In 2008, BST2 was identified as an interferon (IFN) inducible, Vpu sensitive factor that could interfere with the release of HIV-1 progeny virions from the surface of cells [120,121]. In cell types that constitutively express BST2, such as primary CD4+ T-cells, HeLa, and Jurkat cells, Vpu is required for the efficient release of progeny virions from the cell surface. However, Vpu is not required for release of progeny virions in cell types such as 293T and HT1080 cells which do not express BST2 [120,121]. BST2 retains fully formed viruses to the surface of cells by linking them to the cell membrane and to each other [120]. These cell surface attached virions are re-internalized via recycling of BST2. They then traffick to late endosomes/lysosomes for degradation [121,122,123]. Whether BST2 decreases cell-to-cell transmission of HIV-1 is unclear because it has been shown to either reduce cell-to-cell transmission [124,125] or to be unable to inhibit cell-to-cell spread of virus [126,127].
Lentiviruses have evolved different strategies to overcome BST2-mediated restriction. The known antagonists of BST2 include the Vpu protein of HIV-1, envelope (Env) protein of HIV-2 and SIV, and the Nef protein of SIV. HIV-1 Vpu has been shown to reduce the levels of BST2 expression on the cell surface [121,123]. However, the mechanism of downregulation is not entirely clear. Recent reports suggest that Vpu, which is also an integral membrane protein like BST2, targets the TM domain of BST2. However, the site at which Vpu interacts with BST2 is not very clear. Vpu has also been shown to reduce total cellular levels of endogenous as well as exogenously expressed BST2. Several reports suggest involvement of either a beta-TrCP dependent proteasome or endolysosomal pathway in Vpu-mediated degradation of BST2 [128,129,130,131,132]. In contrast to HIV-1 Vpu, HIV-2 Env and SIV Nef interact with the BST2 ectodomain [133] and cytoplasmic tail [134,135], respectively. Furthermore, they may induce downregulation of BST2 from the cell surface without causing degradation [133,134,135].
HIV-1 Vpu exhibits species-specific activity against BST2. It can overcome the activity of human and chimpanzee BST2, but is ineffective against BST2 from RM, AGM, and mustached monkeys (Cercopithecus cephus) [59,132,134,136,137,138]. Vpu expressed from SIVmus, which infects mustached monkeys, also exhibits species-specific function. It is effective in antagonizing mustached monkey and AGM BST2, but is not active against human BST2 [7,139]. Surprisingly, the Vpu proteins expressed by SIVcpz and SIVrcm lack activity against all BST2 proteins tested [7,139]. Interestingly, the Nef proteins from these viruses downregulate BST2 expression [7]. The SIV Nef also exhibits species-specific activity against BST2. It targets RM, PTM, and AGM BST2 but cannot antagonize human BST2 [134,135,139]. This is due to the absence of a G/DDIWK motif in human BST2, which the SIV Nef targets in the RM protein [134,135].
The importance of counteracting BST2 for persistent viral replication is highlighted by the fact that multiple lentiviral proteins have evolved to antagonize BST2. However, the requirement for Vpu-mediated antagonism of BST2 for HIV-1 replication and pathogenesis still requires additional investigation. This is because Vpu may not be absolutely required for replication. For example, there are primary isolates of HIV-1 that do not express Vpu due to a mutation in its start codon [140,141]. Furthermore, although Vpu and Nef proteins of Group O HIV-1 are poor BST2 antagonists, the viruses still cause AIDS in infected individuals [7]. Vpu has also been shown to enhance virion release even in the absence of cell surface down modulation and intracellular depletion of BST2 [142]. Finally, as indicated previously, there are also observations showing that the absence of Vpu reduces virion release from cells but does not affect cell-to-cell transmission of the virus, which is suggested to be a major mode of HIV-1 transmission in vivo.

4.5. SAMHD1

Myeloid lineage cells such as monocytes, dendritic cells and macrophages are known to be refractory to HIV-1 infection [143,144,145,146]. However, infection of these cells with HIV-1 can be enhanced by preincubating the cells with virus-like particles derived from SIV that express the Vpx protein from either the HIV-2 or SIVsm lineage [144,147]. Vpx, which likely evolved as a result of duplication of the vpr gene [148], has been shown to interact with the DDB1-CUL4-DCAF1 E3 ubiquitin ligase complex and enhance HIV-1 infection of DCs, monocytes, and macrophages by causing proteasomal degradation of a putative restriction factor [144,149,150]. Recently, two independent groups identified sterile alpha motif (SAM) and histidine/aspartic acid (HD) domain containing protein 1 (SAMHD1) as a novel restriction factor that inhibits HIV-1 infection of myeloid cells. They showed that Vpx interacts with SAMHD1, resulting in its degradation via the proteasome [151,152]. Although the biological function of SAMHD1 is not very clear, mutations in SAMHD1 have been associated with Aicardi-Goutieres syndrome (AGS), a genetic encephalopathy with symptoms mimicking viral infection. This disease is characterized by excessive production of IFNα and chronic lymphocytosis [153]. Interestingly, SAMHD1 is induced by IFN and has been proposed to act as a negative regulator of the IFN response [153]. Mutations in other genes such as three prime repair exonuclease 1(TREX1), and components of the endonuclease complex RNase H2 (RNAseH2A, RNAseH2B, and RNAseH2C), have also been linked to AGS [154,155]. However, in contrast to SAMHD1, TREX1 and RNAseH2A have been shown to facilitate HIV-1 infection [156,157]. Since, TREX1 and RNaseH2 complex are involved in nucleic acid metabolism, a similar role for SAMHD1 was suggested. Indeed, two recent reports suggest that the dimeric SAMHD1 core domain has 2’-deoxy-guanosine 5’-triphosphate (dGTP)-stimulated deoxynucleoside triphosphate (dNTP) triphopsphohydrolase activity [158,159]. They showed that SAMHD1 converts dNTPs to deoxynucleotides and triphosphate in an in vitro enzymatic assay. Interestingly, Vpx expression or SAMHD1 depletion increases the amount of dNTPs in macrophages, suggesting that SAMHD1 inhibits HIV-1 infection by decreasing the dNTP pool required for viral cDNA synthesis [160].
Recent reports also suggest that antagonism of SAMHD1 by Vpx occurs in a species-specific manner [61,62]. Human and gibbon SAMHD1 can be degraded by Vpx proteins from HIV-2rod, SIVmac, and SIVsm but not by Vpx from SIVrcm and SIVmnd2. However, all these Vpx proteins can induce degradation of rhesus macaque and mangabey SAMHD1 [62]. Interestingly, none of these Vpx proteins induce degradation of SAMHD1 from gray mouse lemurs, which is the most distantly related primate from humans [62]. Surprisingly, some of the lentiviral Vpr proteins also degrade SAMHD1 [61]. Vpr protein from SIVdeb, which infects De Brazza’s monkeys, can not only degrade SAMHD1 from De Brazza’s monkeys but also from human, SM, AGM, RM, RCM, and mandrills [61]. Similarly, Vpr from SIVmus can also degrade SAMHD1 from diverse primate species [61]. Interestingly, Vpr from SIVagm Grivet (677 strain) only degrades SAMHD1 from AGMs, whereas SIVagmVervet(9648 strain) Vpr is able to degrade SAMHD1 from AGM, RM, RCM, SM, De Brazza’s monkeys, and Mandrills [61]. Overall, these results suggest that primate lentiviruses have evolved strategies to overcome SAMHD1 restriction.
Interestingly, HIV-1 does not have a mechanism to antagonize human SAMHD1, but it still replicates and causes disease in humans. This raises questions about the importance of SAMHD1 mediated restriction of HIV-1 in myeloid cells. Since mutations in SAMHD1 result in increased production of interferon, it can be hypothesized that SAMHD1 mediated restriction of HIV-1 infection in myeloid cells likely prevents an innate immune response. Indeed, forcing HIV-1 to replicate in DCs by providing Vpx in trans results in interferon production [161], suggesting that HIV-1 may not have evolved a mechanism to inhibit SAMHD1 in order to avoid productively infecting myeloid cells and inducing an immune response. However, SIVmac, which causes disease in RMs, expresses Vpx to disrupt RM SAMHD1 restriction activity. Furthermore, new data indicate that Vpx-mediated degradation of SAMHD1 is necessary for macrophage-tropism and robust replication of SIVmne in CD4+ T-cells in PTMs [29]. Therefore, it is not clear why HIV-1 has not evolved a mechanism to counteract SAMHD1 and enhance infection of myeloid cells. Further studies will be necessary to understand the importance of myeloid cell infection for persistent HIV-1 and SIV replication.

4.6. Role of Interferon

Endocytosis of HIV-1 induces IFNα in plasmacytoid dendritic cells via TLR7 recognition of genomic viral RNA [162]. Recently, Manel et al. suggested that an intrinsic HIV-1 recognition mechanism in monocyte-derived dendritic cells plays a role in detection of newly synthesized viral capsid proteins, thereby activating the innate immune response [161]. Interestingly, macrophages may lack expression of a pattern recognition receptor for HIV-1, as these cells can be infected with HIV-1 without triggering an innate immune response [163]. Induction of IFNα results in the expression of many interferon stimulated genes (ISGs) and the establishment of an antiviral state in the cell. Significantly, retroviral restriction factors (i.e., APOBEC3 family proteins, TRIM5α, BST2, and SAMHD1) are upregulated by type I interferons [122,153,164,165,166]. Several studies have also identified additional ISGs that inhibit HIV-1 infection. For example, TRIM22 has been shown to inhibit HIV-1 transcription [167,168], and may disrupt Gag assembly [169]. Interferon inducible transmembrane (IFITM) proteins inhibit HIV-1 infection either by suppressing Gag production (IFITM1) or by interfering with virus entry (IFITM2 and IFITM3) [170]. Type I interferons have also been shown to increase expression of TRIM5α and TRIMcyp in monkey cells. Increased expression of TRIMcyp in OWM cells was also shown to restrict HIV-1 infection [166]. Additionally, early reports suggested that pretreatment of cells with type I interferons inhibits the replication of HIV-1 by interfering with reverse transcription and integration of the provirus [171,172,173,174,175,176,177]. Thus, it is likely that the upregulation of retroviral restriction factors mediates inhibition of HIV-1 replication by interferon. It therefore can be hypothesized that interferon induced restriction factors have forced the lentiviruses to evolve strategies to overcome the inhibitory activities of ISGs in order to infect and persistently replicate in the host.

5. Macaque-Tropic HIV-1

5.1. Rational Design of Macaque Tropic-HIV-1

The identification of retroviral restriction factors and the counter measures used by primate lentiviruses have guided the rational design of macaque-tropic HIV-1 (mtHIV-1) clones (Table 2). HIV-1 can replicate in human cells because its capsid is not recognized by the restriction factor human TRIM5α, and its Vif protein inhibits APOBEC3 protein family members. However, as discussed above, the RM TRIM5α protein blocks HIV-1 infection, and the Vif protein cannot counteract the RM APOBEC3 family of restriction factors. These data suggest that the inability of HIV-1 to replicate in RM cells may be overcome by engineering resistance to TRIM5α and APOBEC3 proteins. Thus, a mtHIV-1 virus was initially developed by substituting the HIV-1 capsid and vif sequences with the corresponding sequences from SIVmac [178]. mtHIV-1, which has 90% of the HIV-1 genome combined with 10% of SIV sequences, replicated robustly in a RM T-cell line and RM PBMCs after in vitro adaptation [178]. However, the replication efficiency of mtHIV-1 in vivo is unknown. To minimize the sequences from SIV, an HIV-1 derivative which carries only the SIVmacvif gene and a short 21 base pair segment from the SIV capsid sequence corresponding to the HIV-1 cylophilin A binding loop was engineered and showed increased infectivity in a CM T-cell line [179]. Only after passaging this virus in the CM T-cell line was an in vitro adapted HIV-1 derivative (NL-DT5R) generated that could replicate well in the CM T-cell line as well as CD8+ T-cells depleted T-cells from both PTMs and RMs. Although NL-DT5R established a productive infection and elicited humoral responses in PTMs, it did not cause CD4+ T-cells depletion or disease [180]. A modified version of this virus with enhanced replication capacity in CM CD4+ T-cells was also generated by splicing a loop between alpha helices 6 and 7 (L6/7) of the SIVmac CA into the corresponding region in HIV-1(NL-DT5R6/7S) [181]. Interestingly, although NL-DT5R also established infection in CMs, viral loads were marginal and disappeared by 4 weeks post-infection [182]. Long-term passaging of both CXCR4- and CCR5-tropic NL-DT5R in CM-derived HSC-F cells resulted in a total of 14 mutations. These mutations were introduced into the parental NL-DT5R clone to generate a clone named MN4-5. A modified MN4-5 clone (MN4-5S) generated by inserting the loop between alpha helices 6 and 7 (L6/7) of the SIVmac CA into the corresponding region in HIV-1 showed enhanced replication compared to the parental NL-DT5R and MN4-5 clones in CM-derived HSC-F cells and CD8+T-cells depleted PBMCs from CMs. Although infection of CMs with MN4-5S resulted in higher viral titer compared to NL-DT5R at 2 to 3 weeks post infection, viremia became undetectable at 6 weeks post infection, partly due to control by CD8+T-cells [182].

5.2. Unique Susceptibility of Pigtailed Macaques to HIV-1 Infection

PTMs are known to be uniquely susceptible to HIV-1 infection [183,184,185,186,187]. Agy et al. first showed that PTMs can be infected with HIV-1 [183]. All the infected animals demonstrated seropositivity against a broad range of HIV-1 proteins. Furthermore, the authors were able to detect viral DNA in PBMCs and were able to re-isolate virus by cocultivation. However, viral loads diminished rapidly after inoculation. In another study, Gartner et al. infected PTMs with autologous cells expressing low amounts HIV-1 [185]. During the first 10 weeks post infection, they were able to recover infectious virus from PBMCs and lymph nodes in 3 out of 4 infected macaques. In one of the infected animals, virus could be re-isolated at 38 and 61 weeks post-infection, suggesting that the animal was persistently infected with HIV-1. Interestingly, in vivo passaging of the virus in PTMs did not select for pathogenic variants. This is probably because PTMs restrict HIV-1 replication to a level that is not sufficient for adaptive mutations to occur. Although HIV-1 replicates more vigorously in newborns, passaging of HIV-1 in newborn PTMs did not result in the emergence of pathogenic variants [188,189]. Despite these outcomes, these studies demonstrated that PTMs are susceptible to HIV-1 infection. We have also observed that PTM PBMCs can be more easily transduced with VSVG pseudotyped HIV-1 than RM PBMCs, suggesting the absence of a post-entry block [190]. Recently, several groups, including ours, have observed that this unique susceptibility of PTMs to HIV-1 infection is due to the absence of the retroviral restriction factor, TRIM5α. Instead PTMs express novel isoforms of TRIM5 (TRIM5θ, which lacks the B30. 2(SPRY) domain and TRIM5η, which has a deletion of the entire exon 7) that do not restrict HIV-1 infection [191]. Furthermore, PTMs express a TRIM5-cyclophilin A fusion protein (TRIMcyp), which resulted from a LINE-mediated retrotransposition of the cyclophilin A cDNA into the untranslated region of exon 8 of the TRIM5 locus [112,118,192,193]. The PTM TRIMcyp protein is similar to the OWM TRIMcyp. However, it does not restrict HIV-1 infection, although it inhibits FIV, HIV-2 and SIVagm [112,118,192,193]. A comparison of the antiviral action of OWM TRIMcyp and PTM TRIMcyp showed that a single amino acid mutation (H69R) in the cyclophilinA domain of PTM TRIMcyp prevents it from interacting with the HIV-1 CA [112].
Table
Table 2. Macaque tropic HIV-1 derivatives.
Table 2. Macaque tropic HIV-1 derivatives.
Clone Name (Backbone)Substitutions MadePassaging of VirusesIn vitro ReplicationIn vivo Replication DiseaseReferences
stHIV-1 (NL4-3)SIVmac239 capsid and vifYesReplicated robustly in RM T-cells (221) after in vitro passaging??[178]
NL-DT5R (NL4-3)Cyclophilin A binding domain of SIV capsid and vifYesShows spreading infections in CM T-cell line and CD8+ T-cells depleted T-cells PTMsViremia becomes undetectable within 11 and 4 weeks post infection in PTMs and CMs respectivelyNo[179,180]
NL-DT5R6/7S (NL4-3)Modified version of NL-DT5R NoReplicates in CD8+T-cells depleted T-cells from CMs??[181]
MN4-5S (NL4-3)Modified version of NL-DT5RNoReplicates in CD8+T-cells depleted PBMCs from CMsViremia becomes undetectable after 6 weeks post-infection in CMs?[182]
stHIV-1sv, stHIV-12v (NL4-3)SIVmac vif or HIV-2 vif and SHIVKB9 envNoShows spreading infections in PTM PBMCsViremia becomes undetectable after 25 weeks post-infection in PTMsNo[194]
HSIV-vif (NL4-3)SIVmne027 vifNoReplicates efficiently in PTM PBMCsViremia detectable through 2 years post-infection in PTMsNo[190]
HSIV-vif-Yu2 (BRU-YU2)SIVmne027 vifNoReplicates efficiently in PTM PBMCs??[190]
HSIV-vif-AD8 (NL-AD8)SIVmne027 vifNoReplicates efficiently in PTM PBMCs??[190]

5.3. HIV-1 Derivatives with a Minimal SIV vif Substitution

Absence of a post-entry block suggested that inhibition of HIV-1 replication in PTM CD4+ T-cells may be mainly due to APOBEC3 family proteins. This presents an advantage for the development of a minimally modified HIV-1 that can potentially infect and cause AIDS in a macaque species. Since SIVmac Vif can degrade RM APOBEC3G, Hatziioannou et al. constructed an HIV-1 derivative carrying SIVmac vif in place of HIV-1 vif [194]. They also constructed an HIV-1 derivative with an HIV-2 vif gene substitution. Inoculation of PTMs with an admixture of these two viruses resulted in acute infection and viremia persisted for 25 weeks post infection. However, infection was controlled thereafter and it did not result in CD4+T-cell depletion. In a separate experiment, we also constructed an HIV-1 derviative carrying the vif gene from SIV (HSIV-vif) [190], except we used a vif gene derived from the highly pathogenic PTM-adapted SIVmne027 [195,196]. Compared to the previous studies, our study showed extended viral replication through 44 weeks post infection and small rebounds in viral titer at 64 and 72 weeks post-infection in juvenile PTMs. Furthermore, viral DNA could be detected in PBMCs through 90 weeks post-infection, suggesting that the animals were persistently infected for nearly 2 years. Infection of newborn PTMs also resulted in viremia that was detectable through at least 40 weeks post-infection. Finally, we have continued to monitor these animals for more than 3 years. We have observed very low plasma viral loads. Surprisingly, one animal has declining CD4+counts, suggesting disease progression. Interestingly, for reasons that are unclear, newborn PTMs infected as newborns appear to control their infection earlier than those animals infected as juveniles [197].
The reasons for the extended period of replication of HSIV-vif in PTMs compared to the other mtHIV-1 clones is unknown, but could be due to differences in the construction of the viruses. The viruses made by Hatziioannou et al. contained an env gene from RM adapted SHIVKB9 and vif gene from SIVmac239 or HIV-2ROD. Although we used the same back bone, HIV-1 NL4-3, our clone contained a vif allele from PTM adapted SIVmne027 and the NL4-3 envelope. Interestingly, SIVmac239 Vif does not degrade RM A3F [198]. By contrast, we found that SIVmne027 reduces both PTM A3G and A3F in cells and virions [190]. Potentially, this ability of SIVmne027 Vif to effectively degrade both PTM A3G and A3F may have contributed to longer persistent infection in the PTM host. Finally, these studies suggest that mtHIV-1 based on NL4-3 may not be very fit for replication in PTM host. Viral clones based on other HIV-1 variants should be considered in future experiments.

6. Potential Problems with Simian Tropic HIV-1 Derivatives

PTMs infected with different macaque-tropic HIV-1 viruses have not yet developed AIDS, which suggests that there could be other unknown barriers to HIV-1 replication in PTMs. Alternatively, there could be problems associated with the insertion of the SIV vif gene in place of HIV-1 vif gene. Our results suggested that SIV Vif expression from HSIV-vif may be suboptimal for high-level replication in the PTM host. Indeed, we observed that HSIV-vif did not degrade either PTM A3G or A3F as effectively as pathogenic SIVmne027. As a result, HSIV-vif virions incorporated PTM A3G and A3F at levels higher than those found in wild type SIVmne027 virions [190]. Inefficient degradation of A3G and A3F by HSIV-vif could potentially be a problem in the host where A3G levels are upregulated by the host immune response. Several reports suggest that cytokines, chemokines, and cellular activation events can upregulate both A3G and A3F expression [199,200,201,202,203,204,205,206]. It is possible that Vif expression from HSIV-vif may not be sufficient to cope with changes in A3G/A3F expression and hence increased levels of A3G/A3F could reduce the infectivity of progeny virions. Insufficient inhibition of A3G could also result in greater control of HSIV-vif by the cellular immune response as reported by Casartelli et al. [74]. Surprisingly, we did not observe hypermutation in the proviral DNA isolated from PBMCs from infected macaques. However, this could be due to low level of replication of the virus in the host or clearance of hypermutated virus. Finally, our unpublished results suggest that G to A mutated genomes can be isolated from PTM CD4+ T-cells infected with HSIV-vif in vitro, suggesting that the level of Vif expression may not be sufficient to completely overcome PTM A3 family proteins [197].
The absence of virion associated Vif protein is another characteristic distinguishing HSIV-vif from SIVmne027 [190]. This could be because SIV Vif does not incorporate into HIV-1 core particles, or it may be due to a low level of Vif expression from HSIV-vif. Although it is not clear if Vif incorporation into virions is indeed required for pathogenesis of HIV-1 and SIV in vivo, we cannot rule out the importance of SIV Vif incorporation into virions in the pathogenesis of HSIV-vif. Several reports suggest that Vif packaging into virions may not be required for APOBEC3 degradation or viral infectivity in cell culture [207,208,209,210,211,212]. However, other reports suggest Vif packaging could be necessary for functions such as G2 cell cycle arrest, modulation of reverse transcription, or inhibition of IRF3 [213,214,215,216].
Interestingly, passaging of mtHIV-1 viruses in vitro did not generate pathogenic variants. Even after in vitro passage, the mtHIV-1 virus generated by Kamada et al., showed limited replication capacity in the PTM host [180]. Additional attempts to adapt this virus (NL-DT5R) to CMs did not enhance replication in this alternate species [182], which could be because this virus had poor replication capacity to begin with. All these studies clearly demonstrate that even though these viruses were engineered to overcome specific innate restriction factors, they did not result in high levels of replication in the host. Additionally, the observations that CD8+ T-cell depletion caused rebounds in plasma viral loads and that neutralizing antibodies were generated against stHIV-1, support a hypothesis that these viruses may not overcome adaptive immunity in the host [194]. However, in the Igarashi et al. study, CD8+ T-cell depletion did not enhance viral loads in PTMs [180]. Finally, since in vitro passaging did not result in pathogenic variants, we envision that passaging of HSIV-vif in vivo might select for escape variants that would avoid recognition by cytotoxic T-cells and neutralizing antibodies.
An alternative hypothesis to the inability of these viruses to escape from adaptive immune responses is that they may still be susceptible to restriction by innate immune factors. The rationale behind the prototype mtHIV-1 clones addressed escape from TRIM5α and A3 proteins, but not other possible restriction factors. For example, none of the viruses have included a determinant that allows the viruses to evade the inhibitory effects of BST2. Identification of BST2 as a species-specific retroviral restriction factor also suggests that additional modifications to mtHIV-1 derivatives, such as insertion of the SIV nef gene in place of the HIV-1 nef, may promote its replication in the PTM host. On the other hand, substitution of the HIV-1 nef with SIV nef may not be necessary because SHIV chimeric viruses with HIV-1 nef genes are still pathogenic [217,218,219]. Another possibility is that although Vpx is not required for HIV-1 infection of humans, it may be required for infection of macaques. Indeed, Vpx deletion mutants of SIVs as well as mutants that fail to inhibit SAMHD1 are attenuated in macaques compared to wild type SIVs, suggesting the importance of Vpx in pathogenesis [26,29,30].
Viral infections result in interferon induction but it is unknown how mtHIV-1 derivatives respond to interferon induction in vivo. We have observed that IFNα potently inhibits replication of HSIV-vif based on NL4-3 in PTM CD4+ T-cells, while it has little effect on replication of pathogenic SIVmne027 [220,221]. Thus, it is possible that HSIV-vif does not effectively overcome IFN induced restrictions in PTM CD4+ T-cells in the host, resulting in control of viral replication. It will be important to determine whether HSIV-vif or other mtHIV-1 can evolve resistance to IFN-induced restriction and to define the cellular factors mediating inhibition.

7. Concluding Remarks

Significant progress has been made toward understanding the innate barriers that influence cross-species transmission of lentiviruses. Although HIV-1 derivatives that can infect macaques have been developed, none has yet caused disease. However, these chimeras have helped us define key blocks to transmission and persistent infection. Low level of replication of macaque-tropic HIV-1 chimeras in vivo suggests that there are likely additional restrictions to replication that remain to be identified. Future studies should address the lack of correlation between viral replication in vitro in macaque T-cells and in vivo. An in depth understanding of the factors that control replication of macaque-tropic HIV-1 clones in vivo should provide insights into the role of the innate immune response in cross-species transmission and further progress toward a nonhuman primate model of HIV-1 infection and disease.

Acknowledgments

J.T.K. is supported by grants AI083095 and AI099007 from the National Institutes of Health.

References

  1. Keele, B.F.; Jones, J.H.; Terio, K.A.; Estes, J.D.; Rudicell, R.S.; Wilson, M.L.; Li, Y.; Learn, G.H.; Beasley, T.M.; Schumacher-Stankey, J.; et al. Increased mortality and aids-like immunopathology in wild chimpanzees infected with sivcpz. Nature 2009, 460, 515–519. [Google Scholar]
  2. Fultz, P.N. Nonhuman primate models for aids. Clin. Infect. Dis. Off. Publ. Infect. Dis. Soc. Am. 1993, 17, S230–S235. [Google Scholar]
  3. Gao, F.; Bailes, E.; Robertson, D.L.; Chen, Y.; Rodenburg, C.M.; Michael, S.F.; Cummins, L.B.; Arthur, L.O.; Peeters, M.; Shaw, G.M.; et al. Origin of Hiv-1 in the chimpanzee pan troglodytes troglodytes. Nature 1999, 397, 436–4414. [Google Scholar]
  4. Keele, B.F.; Van Heuverswyn, F.; Li, Y.; Bailes, E.; Takehisa, J.; Santiago, M.L.; Bibollet-Ruche, F.; Chen, Y.; Wain, L.V.; Liegeois, F.; et al. Chimpanzee reservoirs of pandemic and nonpandemic hiv-1. Science 2006, 313, 523–526. [Google Scholar]
  5. Plantier, J.C.; Leoz, M.; Dickerson, J.E.; De Oliveira, F.; Cordonnier, F.; Lemee, V.; Damond, F.; Robertson, D.L.; Simon, F. A new human immunodeficiency virus derived from gorillas. Nat. Med. 2009, 15, 871–872. [Google Scholar]
  6. Bailes, E.; Gao, F.; Bibollet-Ruche, F.; Courgnaud, V.; Peeters, M.; Marx, P.A.; Hahn, B.H.; Sharp, P.M. Hybrid origin of siv in chimpanzees. Science 2003, 300, 1713. [Google Scholar]
  7. Sauter, D.; Schindler, M.; Specht, A.; Landford, W.N.; Munch, J.; Kim, K.A.; Votteler, J.; Schubert, U.; Bibollet-Ruche, F.; Keele, B.F.; et al. Tetherin-driven adaptation of vpu and nef function and the evolution of pandemic and nonpandemic hiv-1 strains. Cell Host Micr. 2009, 6, 409–421. [Google Scholar] [CrossRef]
  8. Gaddis, N.C.; Sheehy, A.M.; Ahmad, K.M.; Swanson, C.M.; Bishop, K.N.; Beer, B.E.; Marx, P.A.; Gao, F.; Bibollet-Ruche, F.; Hahn, B.H.; et al. Further investigation of simian immunodeficiency virus vif function in human cells. J. Virol. 2004, 78, 12041–12046. [Google Scholar]
  9. Kratovac, Z.; Virgen, C.A.; Bibollet-Ruche, F.; Hahn, B.H.; Bieniasz, P.D.; Hatziioannou, T. Primate lentivirus capsid sensitivity to trim5 proteins. J. Virol. 2008, 82, 6772–6777. [Google Scholar]
  10. Lim, E.S.; Malik, H.S.; Emerman, M. Ancient adaptive evolution of tetherin shaped the functions of vpu and nef in human immunodeficiency virus and primate lentiviruses. J. Virol. 2010, 84, 7124–7134. [Google Scholar] [CrossRef]
  11. Hirsch, V.M.; Olmsted, R.A.; Murphey-Corb, M.; Purcell, R.H.; Johnson, P.R. An african primate lentivirus (sivsm) closely related to hiv-2. Nature 1989, 339, 389–392. [Google Scholar]
  12. Chen, Z.; Telfier, P.; Gettie, A.; Reed, P.; Zhang, L.; Ho, D.D.; Marx, P.A. Genetic characterization of new west african simian immunodeficiency virus sivsm: Geographic clustering of household-derived siv strains with human immunodeficiency virus type 2 subtypes and genetically diverse viruses from a single feral sooty mangabey troop. J. Virol. 1996, 70, 3617–3627. [Google Scholar]
  13. Santiago, M.L.; Range, F.; Keele, B.F.; Li, Y.; Bailes, E.; Bibollet-Ruche, F.; Fruteau, C.; Noe, R.; Peeters, M.; Brookfield, J.F.; et al. Simian immunodeficiency virus infection in free-ranging sooty mangabeys (cercocebus atys atys) from the tai forest, cote d'ivoire: Implications for the origin of epidemic human immunodeficiency virus type 2. J. Virol. 2005, 79, 12515–12527. [Google Scholar]
  14. Allan, J.S.; Short, M.; Taylor, M.E.; Su, S.; Hirsch, V.M.; Johnson, P.R.; Shaw, G.M.; Hahn, B.H. Species-specific diversity among simian immunodeficiency viruses from african green monkeys. J. Virol. 1991, 65, 2816–2828. [Google Scholar]
  15. Muller, M.C.; Saksena, N.K.; Nerrienet, E.; Chappey, C.; Herve, V.M.; Durand, J.P.; Legal-Campodonico, P.; Lang, M.C.; Digoutte, J.P.; Georges, A.J.; et al. Simian immunodeficiency viruses from central and western africa: Evidence for a new species-specific lentivirus in tantalus monkeys. J. Virol. 1993, 67, 1227–1235. [Google Scholar]
  16. VandeWoude, S.; Apetrei, C. Going wild: Lessons from naturally occurring t-lymphotropic lentiviruses. Clin. Microbiol. Rev. 2006, 19, 728–762. [Google Scholar] [CrossRef]
  17. Hirsch, V.M.; Dapolito, G.A.; Goldstein, S.; McClure, H.; Emau, P.; Fultz, P.N.; Isahakia, M.; Lenroot, R.; Myers, G.; Johnson, P.R. A distinct african lentivirus from sykes' monkeys. J. Virol. 1993, 67, 1517–1528. [Google Scholar]
  18. Daniel, M.D.; Letvin, N.L.; King, N.W.; Kannagi, M.; Sehgal, P.K.; Hunt, R.D.; Kanki, P.J.; Essex, M.; Desrosiers, R.C. Isolation of t-cell tropic htlv-iii-like retrovirus from macaques. Science 1985, 228, 1201–1204. [Google Scholar]
  19. Letvin, N.L.; Daniel, M.D.; Sehgal, P.K.; Desrosiers, R.C.; Hunt, R.D.; Waldron, L.M.; MacKey, J.J.; Schmidt, D.K.; Chalifoux, L.V.; King, N.W. Induction of aids-like disease in macaque monkeys with t-cell tropic retrovirus stlv-iii. Science 1985, 230, 71–73. [Google Scholar]
  20. Kestler, H.W., 3rd; Naidu, Y.N.; Kodama, T.; King, N.W.; Daniel, M.D.; Li, Y.; Desrosiers, R.C. Use of infectious molecular clones of simian immunodeficiency virus for pathogenesis studies. J. Med. Primatology 1989, 18, 305–309. [Google Scholar]
  21. Naidu, Y.M.; Kestler, H.W., 3rd; Li, Y.; Butler, C.V.; Silva, D.P.; Schmidt, D.K.; Troup, C.D.; Sehgal, P.K.; Sonigo, P.; Daniel, M.D.; et al. Characterization of infectious molecular clones of simian immunodeficiency virus (sivmac) and human immunodeficiency virus type 2: Persistent infection of rhesus monkeys with molecularly cloned sivmac. J. Virol. 1988, 62, 4691–4696. [Google Scholar]
  22. Benveniste, R.E.; Morton, W.R.; Clark, E.A.; Tsai, C.C.; Ochs, H.D.; Ward, J.M.; Kuller, L.; Knott, W.B.; Hill, R.W.; Gale, M.J.; et al. Inoculation of baboons and macaques with simian immunodeficiency virus/mne, a primate lentivirus closely related to human immunodeficiency virus type 2. J. Virol. 1988, 62, 2091–2101. [Google Scholar]
  23. Lewis, M.G.; Zack, P.M.; Elkins, W.R.; Jahrling, P.B. Infection of rhesus and cynomolgus macaques with a rapidly fatal siv (sivsmm/pbj) isolate from sooty mangabeys. AIDS Res. Hum. Retroviruses 1992, 8, 1631–1639. [Google Scholar] [CrossRef]
  24. Kestler, H.W., 3rd; Ringler, D.J.; Mori, K.; Panicali, D.L.; Sehgal, P.K.; Daniel, M.D.; Desrosiers, R.C. Importance of the nef gene for maintenance of high virus loads and for development of aids. Cell 1991, 65, 651–662. [Google Scholar] [CrossRef]
  25. Whatmore, A.M.; Cook, N.; Hall, G.A.; Sharpe, S.; Rud, E.W.; Cranage, M.P. Repair and evolution of nef in vivo modulates simian immunodeficiency virus virulence. J. Virol. 1995, 69, 5117–5123. [Google Scholar]
  26. Gibbs, J.S.; Lackner, A.A.; Lang, S.M.; Simon, M.A.; Sehgal, P.K.; Daniel, M.D.; Desrosiers, R.C. Progression to aids in the absence of a gene for vpr or vpx. J. Virol. 1995, 69, 2378–2383. [Google Scholar]
  27. Lang, S.M.; Weeger, M.; Stahl-Hennig, C.; Coulibaly, C.; Hunsmann, G.; Muller, J.; Muller-Hermelink, H.; Fuchs, D.; Wachter, H.; Daniel, M.M.; et al. Importance of vpr for infection of rhesus monkeys with simian immunodeficiency virus. J. Virol. 1993, 67, 902–912. [Google Scholar]
  28. Kimata, J.T. Hiv-1 fitness and disease progression: Insights from the siv-macaque model. Curr. HIV Res. 2006, 4, 65–77. [Google Scholar] [CrossRef]
  29. Belshan, M.; Kimata, J.T.; Brown, C.; Cheng, X.; McCulley, A.; Larsen, A.; Thippeshappa, R.; Hodara, V.; Giavedoni, L.; Hirsch, V.; et al. Vpx is critical for sivmne infection of pigtail macaques. Retrovirology 2012, 9, 32. [Google Scholar] [CrossRef]
  30. Hirsch, V.M.; Sharkey, M.E.; Brown, C.R.; Brichacek, B.; Goldstein, S.; Wakefield, J.; Byrum, R.; Elkins, W.R.; Hahn, B.H.; Lifson, J.D.; et al. Vpx is required for dissemination and pathogenesis of siv(sm) pbj: Evidence of macrophage-dependent viral amplification. Nat. Med. 1998, 4, 1401–1408. [Google Scholar]
  31. Ambrose, Z.; KewalRamani, V.N.; Bieniasz, P.D.; Hatziioannou, T. Hiv/aids: In search of an animal model. Trends Biotechnol. 2007, 25, 333–337. [Google Scholar] [CrossRef]
  32. Human Retroviruses and Aids: A Compilation and Analysis of Nucleic acid and Amino Acid Sequences; Myers, G.B.; Rabson, A.B.; Smith, T.F.; Wong-Staal, F. (Eds.) Theoretical Biology and Biophysics Group T- 10, K7 10, Los Alamos National Laboratory: Los Alamos, NM, USA, 1990.
  33. Isaka, Y.; Sato, A.; Kawauchi, S.; Suyama, A.; Miki, S.; Hayami, M.; Fujiwara, T. Construction of the chimeric reverse transcriptase of simian immunodeficiency virus sensitive to nonnucleoside reverse transcriptase inhibitor. Microbiol. Immunol. 1998, 42, 195–202. [Google Scholar]
  34. Ambrose, Z.; Boltz, V.; Palmer, S.; Coffin, J.M.; Hughes, S.H.; Kewalramani, V.N. In vitro characterization of a simian immunodeficiency virus-human immunodeficiency virus (hiv) chimera expressing hiv type 1 reverse transcriptase to study antiviral resistance in pigtail macaques. J. Virol. 2004, 78, 13553–13561. [Google Scholar]
  35. Pantophlet, R.; Burton, D.R. Gp120: Target for neutralizing hiv-1 antibodies. Annu. Rev. Immunol. 2006, 24, 739–769. [Google Scholar] [CrossRef]
  36. Shibata, R.; Kawamura, M.; Sakai, H.; Hayami, M.; Ishimoto, A.; Adachi, A. Generation of a chimeric human and simian immunodeficiency virus infectious to monkey peripheral blood mononuclear cells. J. Virol. 1991, 65, 3514–3520. [Google Scholar]
  37. Yuste, E.; Sanford, H.B.; Carmody, J.; Bixby, J.; Little, S.; Zwick, M.B.; Greenough, T.; Burton, D.R.; Richman, D.D.; Desrosiers, R.C.; et al. Simian immunodeficiency virus engrafted with human immunodeficiency virus type 1 (hiv-1)-specific epitopes: Replication, neutralization, and survey of hiv-1-positive plasma. J. Virol. 2006, 80, 3030–3041. [Google Scholar]
  38. Luciw, P.A.; Pratt-Lowe, E.; Shaw, K.E.; Levy, J.A.; Cheng-Mayer, C. Persistent infection of rhesus macaques with t-cell-line-tropic and macrophage-tropic clones of simian/human immunodeficiency viruses (shiv). Proc. Natl. Acad. Sci. USA 1995, 92, 7490–7494. [Google Scholar]
  39. Reimann, K.A.; Li, J.T.; Veazey, R.; Halloran, M.; Park, I.W.; Karlsson, G.B.; Sodroski, J.; Letvin, N.L. A chimeric simian/human immunodeficiency virus expressing a primary patient human immunodeficiency virus type 1 isolate env causes an aids-like disease after in vivo passage in rhesus monkeys. J. Virol. 1996, 70, 6922–6928. [Google Scholar]
  40. Joag, S.V.; Li, Z.; Foresman, L.; Stephens, E.B.; Zhao, L.J.; Adany, I.; Pinson, D.M.; McClure, H.M.; Narayan, O. Chimeric simian/human immunodeficiency virus that causes progressive loss of cd4+ t cells and aids in pig-tailed macaques. J. Virol. 1996, 70, 3189–3197. [Google Scholar]
  41. Harouse, J.M.; Gettie, A.; Tan, R.C.; Blanchard, J.; Cheng-Mayer, C. Distinct pathogenic sequela in rhesus macaques infected with ccr5 or cxcr4 utilizing shivs. Science 1999, 284, 816–819. [Google Scholar]
  42. Tan, R.C.; Harouse, J.M.; Gettie, A.; Cheng-Mayer, C. In vivo adaptation of shiv(sf162): Chimeric virus expressing a nsi, ccr5-specific envelope protein. J. Med. Primatology 1999, 28, 164–168. [Google Scholar] [CrossRef]
  43. Song, R.J.; Chenine, A.L.; Rasmussen, R.A.; Ruprecht, C.R.; Mirshahidi, S.; Grisson, R.D.; Xu, W.; Whitney, J.B.; Goins, L.M.; Ong, H.; et al. Molecularly cloned shiv-1157ipd3n4: A highly replication- competent, mucosally transmissible r5 simian-human immunodeficiency virus encoding hiv clade c env. J. Virol. 2006, 80, 8729–8738. [Google Scholar]
  44. Shibata, R.; Igarashi, T.; Haigwood, N.; Buckler-White, A.; Ogert, R.; Ross, W.; Willey, R.; Cho, M.W.; Martin, M.A. Neutralizing antibody directed against the hiv-1 envelope glycoprotein can completely block hiv-1/siv chimeric virus infections of macaque monkeys. Nat. Med. 1999, 5, 204–210. [Google Scholar] [CrossRef]
  45. Nishimura, Y.; Igarashi, T.; Haigwood, N.L.; Sadjadpour, R.; Donau, O.K.; Buckler, C.; Plishka, R.J.; Buckler-White, A.; Martin, M.A. Transfer of neutralizing igg to macaques 6 h but not 24 h after shiv infection confers sterilizing protection: Implications for hiv-1 vaccine development. Proc. Natl. Acad. Sci. USA 2003, 100, 15131–15136. [Google Scholar]
  46. Mascola, J.R.; Stiegler, G.; VanCott, T.C.; Katinger, H.; Carpenter, C.B.; Hanson, C.E.; Beary, H.; Hayes, D.; Frankel, S.S.; Birx, D.L.; et al. Protection of macaques against vaginal transmission of a pathogenic hiv-1/siv chimeric virus by passive infusion of neutralizing antibodies. Nat. Med. 2000, 6, 207–210. [Google Scholar]
  47. Baba, T.W.; Liska, V.; Hofmann-Lehmann, R.; Vlasak, J.; Xu, W.; Ayehunie, S.; Cavacini, L.A.; Posner, M.R.; Katinger, H.; Stiegler, G.; et al. Human neutralizing monoclonal antibodies of the igg1 subtype protect against mucosal simian-human immunodeficiency virus infection. Nat. Med. 2000, 6, 200–206. [Google Scholar]
  48. Amara, R.R.; Villinger, F.; Altman, J.D.; Lydy, S.L.; O'Neil, S.P.; Staprans, S.I.; Montefiori, D.C.; Xu, Y.; Herndon, J.G.; Wyatt, L.S.; et al. Control of a mucosal challenge and prevention of aids by a multiprotein DNA/mva vaccine. Science 2001, 292, 69–74. [Google Scholar]
  49. Matano, T.; Kano, M.; Nakamura, H.; Takeda, A.; Nagai, Y. Rapid appearance of secondary immune responses and protection from acute cd4 depletion after a highly pathogenic immunodeficiency virus challenge in macaques vaccinated with a DNA prime/sendai virus vector boost regimen. J. Virol. 2001, 75, 11891–11896. [Google Scholar]
  50. Lederman, M.M.; Veazey, R.S.; Offord, R.; Mosier, D.E.; Dufour, J.; Mefford, M.; Piatak, M., Jr.; Lifson, J.D.; Salkowitz, J.R.; Rodriguez, B.; et al. Prevention of vaginal shiv transmission in rhesus macaques through inhibition of ccr5. Science 2004, 306, 485–487. [Google Scholar]
  51. Uberla, K.; Stahl-Hennig, C.; Bottiger, D.; Matz-Rensing, K.; Kaup, F.J.; Li, J.; Haseltine, W.A.; Fleckenstein, B.; Hunsmann, G.; Oberg, B.; et al. Animal model for the therapy of acquired immunodeficiency syndrome with reverse transcriptase inhibitors. Proc. Natl. Acad. Sci. USA 1995, 92, 8210–8214. [Google Scholar]
  52. Balzarini, J.; De Clercq, E.; Uberla, K. Siv/hiv-1 hybrid virus expressing the reverse transcriptase gene of hiv-1 remains sensitive to hiv-1-specific reverse transcriptase inhibitors after passage in rhesus macaques. Journal of acquired immune deficiency syndromes and human retrovirology : official publication of the International Retrovirology Association 1997, 15, 1–4. [Google Scholar] [CrossRef]
  53. Akiyama, H.; Ido, E.; Akahata, W.; Kuwata, T.; Miura, T.; Hayami, M. Construction and in vivo infection of a new simian/human immunodeficiency virus chimera containing the reverse transcriptase gene and the 3' half of the genomic region of human immunodeficiency virus type 1. J. Gen. Virol. 2003, 84, 1663–1669. [Google Scholar] [CrossRef]
  54. Ambrose, Z.; Palmer, S.; Boltz, V.F.; Kearney, M.; Larsen, K.; Polacino, P.; Flanary, L.; Oswald, K.; Piatak, M., Jr.; Smedley, J.; et al. Suppression of viremia and evolution of human immunodeficiency virus type 1 drug resistance in a macaque model for antiretroviral therapy. J. Virol. 2007, 81, 12145–12155. [Google Scholar]
  55. North, T.W.; Van Rompay, K.K.; Higgins, J.; Matthews, T.B.; Wadford, D.A.; Pedersen, N.C.; Schinazi, R.F. Suppression of virus load by highly active antiretroviral therapy in rhesus macaques infected with a recombinant simian immunodeficiency virus containing reverse transcriptase from human immunodeficiency virus type 1. J. Virol. 2005, 79, 7349–7354. [Google Scholar]
  56. Hofman, M.J.; Higgins, J.; Matthews, T.B.; Pedersen, N.C.; Tan, C.; Schinazi, R.F.; North, T.W. Efavirenz therapy in rhesus macaques infected with a chimera of simian immunodeficiency virus containing reverse transcriptase from human immunodeficiency virus type 1. Antimicrob. Agents Chemother. 2004, 48, 3483–3490. [Google Scholar]
  57. Sawyer, S.L.; Emerman, M.; Malik, H.S. Ancient adaptive evolution of the primate antiviral DNA-editing enzyme apobec3g. PLoS Biol. 2004, 2, E275. [Google Scholar] [CrossRef] [Green Version]
  58. Sawyer, S.L.; Wu, L.I.; Emerman, M.; Malik, H.S. Positive selection of primate trim5alpha identifies a critical species-specific retroviral restriction domain. Proc. Natl. Acad. Sci. USA 2005, 102, 2832–2837. [Google Scholar]
  59. McNatt, M.W.; Zang, T.; Hatziioannou, T.; Bartlett, M.; Fofana, I.B.; Johnson, W.E.; Neil, S.J.; Bieniasz, P.D. Species-specific activity of hiv-1 vpu and positive selection of tetherin transmembrane domain variants. PLoS Pathog. 2009, 5, e1000300. [Google Scholar]
  60. Liu, H.L.; Wang, Y.Q.; Liao, C.H.; Kuang, Y.Q.; Zheng, Y.T.; Su, B. Adaptive evolution of primate trim5alpha, a gene restricting hiv-1 infection. Gene 2005, 362, 109–116. [Google Scholar] [CrossRef]
  61. Lim, E.S.; Fregoso, O.I.; McCoy, C.O.; Matsen, F.A.; Malik, H.S.; Emerman, M. The ability of primate lentiviruses to degrade the monocyte restriction factor samhd1 preceded the birth of the viral accessory protein vpx. Cell Host Microb. 2012, 11, 194–204. [Google Scholar] [CrossRef]
  62. Laguette, N.; Rahm, N.; Sobhian, B.; Chable-Bessia, C.; Munch, J.; Snoeck, J.; Sauter, D.; Switzer, W.M.; Heneine, W.; Kirchhoff, F.; et al. Evolutionary and functional analyses of the interaction between the myeloid restriction factor samhd1 and the lentiviral vpx protein. Cell Host Microb. 2012, 11, 205–217. [Google Scholar] [CrossRef]
  63. Zhang, C.; de Silva, S.; Wang, J.H.; Wu, L. Co-evolution of primate samhd1 and lentivirus vpx leads to the loss of the vpx gene in hiv-1 ancestor. PloS One 2012, 7, e37477. [Google Scholar]
  64. Jarmuz, A.; Chester, A.; Bayliss, J.; Gisbourne, J.; Dunham, I.; Scott, J.; Navaratnam, N. An anthropoid-specific locus of orphan c to u rna-editing enzymes on chromosome 22. Genomics 2002, 79, 285–296. [Google Scholar]
  65. Sheehy, A.M.; Gaddis, N.C.; Choi, J.D.; Malim, M.H. Isolation of a human gene that inhibits hiv-1 infection and is suppressed by the viral vif protein. Nature 2002, 418, 646–650. [Google Scholar]
  66. Sheehy, A.M.; Gaddis, N.C.; Malim, M.H. The antiretroviral enzyme apobec3g is degraded by the proteasome in response to hiv-1 vif. Nat. Med. 2003, 9, 1404–1407. [Google Scholar] [CrossRef]
  67. Strebel, K.; Khan, M.A. Apobec3g encapsidation into hiv-1 virions: Which rna is it? Retrovirology 2008, 5, 55. [Google Scholar] [CrossRef]
  68. Xu, H.; Chertova, E.; Chen, J.; Ott, D.E.; Roser, J.D.; Hu, W.S.; Pathak, V.K. Stoichiometry of the antiviral protein apobec3g in hiv-1 virions. Virology 2007, 360, 247–256. [Google Scholar] [CrossRef]
  69. Bishop, K.N.; Holmes, R.K.; Sheehy, A.M.; Davidson, N.O.; Cho, S.-J.; Malim, M.H. Cytidine deamination of retroviral DNA by diverse apobec proteins. Curr. Biol. 2004, 14, 1392–1396. [Google Scholar]
  70. Harris, R.S.; Bishop, K.N.; Sheehy, A.M.; Craig, H.M.; Petersen-Mahrt, S.K.; Watt, I.N.; Neuberger, M.S.; Malim, M.H. DNA deamination mediates innate immunity to retroviral infection. Cell 2003, 113, 803–809. [Google Scholar] [CrossRef]
  71. Mangeat, B.; Turelli, P.; Caron, G.; Friedli, M.; Perrin, L.; Trono, D. Broad antiretroviral defence by human apobec3g through lethal editing of nascent reverse transcripts. Nature 2003, 424, 99–103. [Google Scholar]
  72. Zhang, H.; Yang, B.; Pomerantz, R.J.; Zhang, C.; Arunachalam, S.C.; Gao, L. The cytidine deaminase cem15 induces hypermutation in newly synthesized hiv-1 DNA. Nature 2003, 424, 94–98. [Google Scholar]
  73. Yang, B.; Chen, K.; Zhang, C.; Huang, S.; Zhang, H. Virion-associated uracil DNA glycosylase-2 and apurinic/apyrimidinic endonuclease are involved in the degradation of apobec3g-edited nascent hiv-1 DNA. J. Biol. Chem. 2007, 282, 11667–11675. [Google Scholar]
  74. Casartelli, N.; Guivel-Benhassine, F.; Bouziat, R.; Brandler, S.; Schwartz, O.; Moris, A. The antiviral factor apobec3g improves ctl recognition of cultured hiv-infected t cells. J. Exp. Med. 2010, 207, 39–49. [Google Scholar] [CrossRef]
  75. Liddament, M.T.; Brown, W.L.; Schumacher, A.J.; Harris, R.S. Apobec3f properties and hypermutation preferences indicate activity against hiv-1 in vivo. Curr. Biol. CB 2004, 14, 1385–1391. [Google Scholar] [CrossRef]
  76. Wiegand, H.L.; Doehle, B.P.; Bogerd, H.P.; Cullen, B.R. A second human antiretroviral factor, apobec3f, is suppressed by the hiv-1 and hiv-2 vif proteins. EMBO J. 2004, 23, 2451–2458. [Google Scholar] [CrossRef]
  77. Zheng, Y.H.; Irwin, D.; Kurosu, T.; Tokunaga, K.; Sata, T.; Peterlin, B.M. Human apobec3f is another host factor that blocks human immunodeficiency virus type 1 replication. J. Virol. 2004, 78, 6073–6076. [Google Scholar]
  78. Bourara, K.; Liegler, T.J.; Grant, R.M. Target cell apobec3c can induce limited g-to-a mutation in hiv-1. PLoS Pathog. 2007, 3, 1477–1485. [Google Scholar]
  79. Dang, Y.; Wang, X.; Esselman, W.J.; Zheng, Y.H. Identification of apobec3de as another antiretroviral factor from the human apobec family. J. Virol. 2006, 80, 10522–10533. [Google Scholar] [CrossRef]
  80. Bishop, K.N.; Verma, M.; Kim, E.Y.; Wolinsky, S.M.; Malim, M.H. Apobec3g inhibits elongation of hiv-1 reverse transcripts. PLoS Pathog. 2008, 4, e1000231. [Google Scholar] [CrossRef]
  81. Holmes, R.K.; Koning, F.A.; Bishop, K.N.; Malim, M.H. Apobec3f can inhibit the accumulation of hiv-1 reverse transcription products in the absence of hypermutation. Comparisons with apobec3g. J. Biol. Chem. 2007, 282, 2587–2595. [Google Scholar]
  82. Bishop, K.N.; Holmes, R.K.; Malim, M.H. Antiviral potency of apobec proteins does not correlate with cytidine deamination. J. Virol. 2006, 80, 8450–8458. [Google Scholar]
  83. Conticello, S.G.; Harris, R.S.; Neuberger, M.S. The vif protein of hiv triggers degradation of the human antiretroviral DNA deaminase apobec3g. Curr. Biol. CB 2003, 13, 2009–2013. [Google Scholar] [CrossRef]
  84. Kobayashi, M.; Takaori-Kondo, A.; Miyauchi, Y.; Iwai, K.; Uchiyama, T. Ubiquitination of apobec3g by an hiv-1 vif-cullin5-elongin b-elongin c complex is essential for vif function. J. Biol. Chem. 2005, 280, 18573–18578. [Google Scholar]
  85. Marin, M.; Rose, K.M.; Kozak, S.L.; Kabat, D. Hiv-1 vif protein binds the editing enzyme apobec3g and induces its degradation. Nat. Med. 2003, 9, 1398–1403. [Google Scholar] [CrossRef]
  86. Mehle, A.; Strack, B.; Ancuta, P.; Zhang, C.; McPike, M.; Gabuzda, D. Vif overcomes the innate antiviral activity of apobec3g by promoting its degradation in the ubiquitin-proteasome pathway. J. Biol. Chem. 2004, 279, 7792–7798. [Google Scholar]
  87. Yu, X.; Yu, Y.; Liu, B.; Luo, K.; Kong, W.; Mao, P.; Yu, X.F. Induction of apobec3g ubiquitination and degradation by an hiv-1 vif-cul5-scf complex. Science 2003, 302, 1056–1060. [Google Scholar]
  88. Kao, S.; Khan, M.A.; Miyagi, E.; Plishka, R.; Buckler-White, A.; Strebel, K. The human immunodeficiency virus type 1 vif protein reduces intracellular expression and inhibits packaging of apobec3g (cem15), a cellular inhibitor of virus infectivity. J. Virol. 2003, 77, 11398–11407. [Google Scholar]
  89. Stopak, K.; de Noronha, C.; Yonemoto, W.; Greene, W.C. Hiv-1 vif blocks the antiviral activity of apobec3g by impairing both its translation and intracellular stability. Mol. cell 2003, 12, 591–601. [Google Scholar]
  90. Mariani, R.; Chen, D.; Schrofelbauer, B.; Navarro, F.; Konig, R.; Bollman, B.; Munk, C.; Nymark-McMahon, H.; Landau, N.R. Species-specific exclusion of apobec3g from hiv-1 virions by vif. Cell 2003, 114, 21–31. [Google Scholar] [CrossRef]
  91. Bogerd, H.P.; Doehle, B.P.; Wiegand, H.L.; Cullen, B.R. A single amino acid difference in the host apobec3g protein controls the primate species specificity of hiv type 1 virion infectivity factor. Proc. Natl. Acad. Sci. USA 2004, 101, 3770–3774. [Google Scholar]
  92. Mangeat, B.; Turelli, P.; Liao, S.; Trono, D. A single amino acid determinant governs the species-specific sensitivity of apobec3g to vif action. J. Biol. Chem. 2004, 279, 14481–14483. [Google Scholar]
  93. Schrofelbauer, B.; Chen, D.; Landau, N.R. A single amino acid of apobec3g controls its species-specific interaction with virion infectivity factor (vif). Proc. Natl. Acad. Sci. USA 2004, 101, 3927–3932. [Google Scholar] [CrossRef]
  94. Xu, H.; Svarovskaia, E.S.; Barr, R.; Zhang, Y.; Khan, M.A.; Strebel, K.; Pathak, V.K. A single amino acid substitution in human apobec3g antiretroviral enzyme confers resistance to hiv-1 virion infectivity factor-induced depletion. Proc. Natl. Acad. Sci. USA 2004, 101, 5652–5657. [Google Scholar]
  95. Schrofelbauer, B.; Senger, T.; Manning, G.; Landau, N.R. Mutational alteration of human immunodeficiency virus type 1 vif allows for functional interaction with nonhuman primate apobec3g. J. Virol. 2006, 80, 5984–5991. [Google Scholar] [CrossRef]
  96. Cowan, S.; Hatziioannou, T.; Cunningham, T.; Muesing, M.A.; Gottlinger, H.G.; Bieniasz, P.D. Cellular inhibitors with fv1-like activity restrict human and simian immunodeficiency virus tropism. Proc. Natl. Acad. Sci. USA 2002, 99, 11914–11919. [Google Scholar]
  97. Munk, C.; Brandt, S.M.; Lucero, G.; Landau, N.R. A dominant block to hiv-1 replication at reverse transcription in simian cells. Proc. Natl. Acad. Sci. USA 2002, 99, 13843–13848. [Google Scholar]
  98. Besnier, C.; Takeuchi, Y.; Towers, G. Restriction of lentivirus in monkeys. Proc. Natl. Acad. Sci. USA 2002, 99, 11920–11925. [Google Scholar]
  99. Hatziioannou, T.; Cowan, S.; Goff, S.P.; Bieniasz, P.D.; Towers, G.J. Restriction of multiple divergent retroviruses by lv1 and ref1. EMBO J. 2003, 22, 385–394. [Google Scholar]
  100. Stremlau, M.; Owens, C.M.; Perron, M.J.; Kiessling, M.; Autissier, P.; Sodroski, J. The cytoplasmic body component trim5alpha restricts hiv-1 infection in old world monkeys. Nature 2004, 427, 848–853. [Google Scholar]
  101. Stremlau, M.; Perron, M.; Lee, M.; Li, Y.; Song, B.; Javanbakht, H.; Diaz-Griffero, F.; Anderson, D.J.; Sundquist, W.I.; Sodroski, J. Specific recognition and accelerated uncoating of retroviral capsids by the trim5alpha restriction factor. Proc. Natl. Acad. Sci. USA 2006, 103, 5514–5519. [Google Scholar]
  102. Anderson, J.L.; Campbell, E.M.; Wu, X.; Vandegraaff, N.; Engelman, A.; Hope, T.J. Proteasome inhibition reveals that a functional preintegration complex intermediate can be generated during restriction by diverse trim5 proteins. J. Virol. 2006, 80, 9754–9760. [Google Scholar]
  103. Wu, X.; Anderson, J.L.; Campbell, E.M.; Joseph, A.M.; Hope, T.J. Proteasome inhibitors uncouple rhesus trim5alpha restriction of hiv-1 reverse transcription and infection. Proc. Natl. Acad. Sci. USA 2006, 103, 7465–7470. [Google Scholar]
  104. Hofmann, W.; Schubert, D.; LaBonte, J.; Munson, L.; Gibson, S.; Scammell, J.; Ferrigno, P.; Sodroski, J. Species-specific, postentry barriers to primate immunodeficiency virus infection. J. Virol. 1999, 73, 10020–10028. [Google Scholar]
  105. Sayah, D.M.; Sokolskaja, E.; Berthoux, L.; Luban, J. Cyclophilin a retrotransposition into trim5 explains owl monkey resistance to hiv-1. Nature 2004, 430, 569–573. [Google Scholar]
  106. Nisole, S.; Lynch, C.; Stoye, J.P.; Yap, M.W. A trim5-cyclophilin a fusion protein found in owl monkey kidney cells can restrict hiv-1. Proc. Natl. Acad. Sci. USA 2004, 101, 13324–13328. [Google Scholar]
  107. Sokolskaja, E.; Sayah, D.M.; Luban, J. Target cell cyclophilin a modulates human immunodeficiency virus type 1 infectivity. J. Virol. 2004, 78, 12800–12808. [Google Scholar]
  108. Hatziioannou, T.; Perez-Caballero, D.; Cowan, S.; Bieniasz, P.D. Cyclophilin interactions with incoming human immunodeficiency virus type 1 capsids with opposing effects on infectivity in human cells. J. Virol. 2005, 79, 176–183. [Google Scholar]
  109. Keckesova, Z.; Ylinen, L.M.; Towers, G.J. Cyclophilin a renders human immunodeficiency virus type 1 sensitive to old world monkey but not human trim5 alpha antiviral activity. J. Virol. 2006, 80, 4683–4690. [Google Scholar]
  110. Berthoux, L.; Sebastian, S.; Sokolskaja, E.; Luban, J. Cyclophilin a is required for trim5{alpha}-mediated resistance to hiv-1 in old world monkey cells. Proc. Natl. Acad. Sci. USA 2005, 102, 14849–14853. [Google Scholar]
  111. Nakayama, E.E.; Miyoshi, H.; Nagai, Y.; Shioda, T. A specific region of 37 amino acid residues in the spry (b30.2) domain of african green monkey trim5alpha determines species-specific restriction of simian immunodeficiency virus sivmac infection. J. Virol. 2005, 79, 8870–8877. [Google Scholar]
  112. Virgen, C.A.; Kratovac, Z.; Bieniasz, P.D.; Hatziioannou, T. Independent genesis of chimeric trim5-cyclophilin proteins in two primate species. Proc. Natl. Acad. Sci. USA 2008, 105, 3563–3568. [Google Scholar]
  113. Nakayama, E.E.; Shioda, T. Anti-retroviral activity of trim5 alpha. Rev. Med. Virol. 2010, 20, 77–92. [Google Scholar] [CrossRef]
  114. Hatziioannou, T.; Perez-Caballero, D.; Yang, A.; Cowan, S.; Bieniasz, P.D. Retrovirus resistance factors ref1 and lv1 are species-specific variants of trim5alpha. Proc. Natl. Acad. Sci. USA 2004, 101, 10774–10779. [Google Scholar]
  115. Keckesova, Z.; Ylinen, L.M.; Towers, G.J. The human and african green monkey trim5alpha genes encode ref1 and lv1 retroviral restriction factor activities. Proc. Natl. Acad. Sci. USA 2004, 101, 10780–10785. [Google Scholar]
  116. Song, B.; Javanbakht, H.; Perron, M.; Park, D.H.; Stremlau, M.; Sodroski, J. Retrovirus restriction by trim5alpha variants from old world and new world primates. J. Virol. 2005, 79, 3930–3937. [Google Scholar]
  117. Wilson, S.J.; Webb, B.L.; Ylinen, L.M.; Verschoor, E.; Heeney, J.L.; Towers, G.J. Independent evolution of an antiviral trimcyp in rhesus macaques. Proc. Natl. Acad. Sci. USA 2008, 105, 3557–3562. [Google Scholar]
  118. Brennan, G.; Kozyrev, Y.; Hu, S.L. Trimcyp expression in old world primates macaca nemestrina and macaca fascicularis. Proc. Natl. Acad. Sci. USA 2008, 105, 3569–3574. [Google Scholar]
  119. Kupzig, S.; Korolchuk, V.; Rollason, R.; Sugden, A.; Wilde, A.; Banting, G. Bst-2/hm1.24 is a raft-associated apical membrane protein with an unusual topology. Traffic 2003, 4, 694–709. [Google Scholar] [CrossRef]
  120. Neil, S.J.; Zang, T.; Bieniasz, P.D. Tetherin inhibits retrovirus release and is antagonized by Hiv-1 vpu. Nature 2008, 451, 425–430. [Google Scholar]
  121. Van Damme, N.; Goff, D.; Katsura, C.; Jorgenson, R.L.; Mitchell, R.; Johnson, M.C.; Stephens, E.B.; Guatelli, J. The interferon-induced protein bst-2 restricts hiv-1 release and is downregulated from the cell surface by the viral vpu protein. Cell Host Microb. 2008, 3, 245–252. [Google Scholar] [CrossRef]
  122. Neil, S.J.; Sandrin, V.; Sundquist, W.I.; Bieniasz, P.D. An interferon-alpha-induced tethering mechanism inhibits hiv-1 and ebola virus particle release but is counteracted by the hiv-1 vpu protein. Cell Host Microb. 2007, 2, 193–203. [Google Scholar] [CrossRef]
  123. Sato, K.; Yamamoto, S.P.; Misawa, N.; Yoshida, T.; Miyazawa, T.; Koyanagi, Y. Comparative study on the effect of human bst-2/tetherin on hiv-1 release in cells of various species. Retrovirology 2009, 6, 53. [Google Scholar] [CrossRef] [Green Version]
  124. Casartelli, N.; Sourisseau, M.; Feldmann, J.; Guivel-Benhassine, F.; Mallet, A.; Marcelin, A.G.; Guatelli, J.; Schwartz, O. Tetherin restricts productive hiv-1 cell-to-cell transmission. PLoS Pathog. 2010, 6, e1000955. [Google Scholar]
  125. Kuhl, B.D.; Sloan, R.D.; Donahue, D.A.; Bar-Magen, T.; Liang, C.; Wainberg, M.A. Tetherin restricts direct cell-to-cell infection of hiv-1. Retrovirology 2010, 7, 115. [Google Scholar] [CrossRef]
  126. Jolly, C.; Booth, N.J.; Neil, S.J. Cell-cell spread of human immunodeficiency virus type 1 overcomes tetherin/bst-2-mediated restriction in t cells. J. Virol. 2010, 84, 12185–12199. [Google Scholar]
  127. Coleman, C.M.; Spearman, P.; Wu, L. Tetherin does not significantly restrict dendritic cell-mediated hiv-1 transmission and its expression is upregulated by newly synthesized hiv-1 nef. Retrovirology 2011, 8, 26. [Google Scholar] [CrossRef]
  128. Bartee, E.; McCormack, A.; Fruh, K. Quantitative membrane proteomics reveals new cellular targets of viral immune modulators. PLoS Pathog. 2006, 2, e107. [Google Scholar] [CrossRef]
  129. Douglas, J.L.; Viswanathan, K.; McCarroll, M.N.; Gustin, J.K.; Fruh, K.; Moses, A.V. Vpu directs the degradation of the human immunodeficiency virus restriction factor bst-2/tetherin via a {beta}trcp-dependent mechanism. J. Virol. 2009, 83, 7931–7947. [Google Scholar]
  130. Mangeat, B.; Gers-Huber, G.; Lehmann, M.; Zufferey, M.; Luban, J.; Piguet, V. Hiv-1 vpu neutralizes the antiviral factor tetherin/bst-2 by binding it and directing its beta-trcp2-dependent degradation. PLoS Pathog. 2009, 5, e1000574. [Google Scholar] [CrossRef]
  131. Mitchell, R.S.; Katsura, C.; Skasko, M.A.; Fitzpatrick, K.; Lau, D.; Ruiz, A.; Stephens, E.B.; Margottin-Goguet, F.; Benarous, R.; Guatelli, J.C. Vpu antagonizes bst-2-mediated restriction of hiv-1 release via beta-trcp and endo-lysosomal trafficking. PLoS Pathog. 2009, 5, e1000450. [Google Scholar] [CrossRef]
  132. Goffinet, C.; Allespach, I.; Homann, S.; Tervo, H.M.; Habermann, A.; Rupp, D.; Oberbremer, L.; Kern, C.; Tibroni, N.; Welsch, S.; et al. Hiv-1 antagonism of cd317 is species specific and involves vpu-mediated proteasomal degradation of the restriction factor. Cell Host Microb. 2009, 5, 285–297. [Google Scholar] [CrossRef]
  133. Le Tortorec, A.; Neil, S.J. Antagonism to and intracellular sequestration of human tetherin by the human immunodeficiency virus type 2 envelope glycoprotein. J. Virol. 2009, 83, 11966–11978. [Google Scholar] [CrossRef]
  134. Jia, B.; Serra-Moreno, R.; Neidermyer, W.; Rahmberg, A.; Mackey, J.; Fofana, I.B.; Johnson, W.E.; Westmoreland, S.; Evans, D.T. Species-specific activity of siv nef and hiv-1 vpu in overcoming restriction by tetherin/bst2. PLoS Pathog. 2009, 5, e1000429. [Google Scholar]
  135. Zhang, F.; Wilson, S.J.; Landford, W.C.; Virgen, B.; Gregory, D.; Johnson, M.C.; Munch, J.; Kirchhoff, F.; Bieniasz, P.D.; Hatziioannou, T. Nef proteins from simian immunodeficiency viruses are tetherin antagonists. Cell Host Microb. 2009, 6, 54–67. [Google Scholar] [CrossRef]
  136. Lim, E.S.; Emerman, M. Simian immunodeficiency virus sivagm from african green monkeys does not antagonize endogenous levels of african green monkey tetherin/bst-2. J. Virol. 2009, 83, 11673–11681. [Google Scholar]
  137. Gupta, R.K.; Hue, S.; Schaller, T.; Verschoor, E.; Pillay, D.; Towers, G.J. Mutation of a single residue renders human tetherin resistant to hiv-1 vpu-mediated depletion. PLoS Pathog. 2009, 5, e1000443. [Google Scholar] [CrossRef]
  138. Rong, L.; Zhang, J.; Lu, J.; Pan, Q.; Lorgeoux, R.P.; Aloysius, C.; Guo, F.; Liu, S.L.; Wainberg, M.A.; Liang, C. The transmembrane domain of bst-2 determines its sensitivity to down-modulation by human immunodeficiency virus type 1 vpu. J. Virol. 2009, 83, 7536–7546. [Google Scholar]
  139. Yang, S.J.; Lopez, L.A.; Hauser, H.; Exline, C.M.; Haworth, K.G.; Cannon, P.M. Anti-tetherin activities in vpu-expressing primate lentiviruses. Retrovirology 2010, 7, 13. [Google Scholar] [CrossRef]
  140. Theodore, T.S.; Englund, G.; Buckler-White, A.; Buckler, C.E.; Martin, M.A.; Peden, K.W. Construction and characterization of a stable full-length macrophage-tropic hiv type 1 molecular clone that directs the production of high titers of progeny virions. AIDS Res. Hum. Retroviruses 1996, 12, 191–194. [Google Scholar] [CrossRef]
  141. Li, Y.; Kappes, J.C.; Conway, J.A.; Price, R.W.; Shaw, G.M.; Hahn, B.H. Molecular characterization of human immunodeficiency virus type 1 cloned directly from uncultured human brain tissue: Identification of replication-competent and -defective viral genomes. J. Virol. 1991, 65, 3973–3985. [Google Scholar]
  142. Miyagi, E.; Andrew, A.J.; Kao, S.; Strebel, K. Vpu enhances hiv-1 virus release in the absence of bst-2 cell surface down-modulation and intracellular depletion. Proc. Natl. Acad. Sci. USA 2009, 106, 2868–2873. [Google Scholar]
  143. Goujon, C.; Arfi, V.; Pertel, T.; Luban, J.; Lienard, J.; Rigal, D.; Darlix, J.L.; Cimarelli, A. Characterization of simian immunodeficiency virus sivsm/human immunodeficiency virus type 2 vpx function in human myeloid cells. J. Virol. 2008, 82, 12335–12345. [Google Scholar]
  144. Goujon, C.; Riviere, L.; Jarrosson-Wuilleme, L.; Bernaud, J.; Rigal, D.; Darlix, J.L.; Cimarelli, A. Sivsm/hiv-2 vpx proteins promote retroviral escape from a proteasome-dependent restriction pathway present in human dendritic cells. Retrovirology 2007, 4, 2. [Google Scholar] [CrossRef]
  145. Kaushik, R.; Zhu, X.; Stranska, R.; Wu, Y.; Stevenson, M. A cellular restriction dictates the permissivity of nondividing monocytes/macrophages to lentivirus and gammaretrovirus infection. Cell Host Microb. 2009, 6, 68–80. [Google Scholar] [CrossRef]
  146. Sharova, N.; Wu, Y.; Zhu, X.; Stranska, R.; Kaushik, R.; Sharkey, M.; Stevenson, M. Primate lentiviral vpx commandeers ddb1 to counteract a macrophage restriction. PLoS Pathog. 2008, 4, e1000057. [Google Scholar] [CrossRef]
  147. Goujon, C.; Jarrosson-Wuilleme, L.; Bernaud, J.; Rigal, D.; Darlix, J.L.; Cimarelli, A. With a little help from a friend: Increasing hiv transduction of monocyte-derived dendritic cells with virion-like particles of siv(mac). Gene Ther. 2006, 13, 991–994. [Google Scholar] [CrossRef]
  148. Ayinde, D.; Maudet, C.; Transy, C.; Margottin-Goguet, F. Limelight on two hiv/siv accessory proteins in macrophage infection: Is vpx overshadowing vpr? Retrovirology 2010, 7, 35. [Google Scholar] [CrossRef] [Green Version]
  149. Srivastava, S.; Swanson, S.K.; Manel, N.; Florens, L.; Washburn, M.P.; Skowronski, J. Lentiviral vpx accessory factor targets vprbp/dcaf1 substrate adaptor for cullin 4 e3 ubiquitin ligase to enable macrophage infection. PLoS Pathog. 2008, 4, e1000059. [Google Scholar] [CrossRef]
  150. Bergamaschi, A.; Ayinde, D.; David, A.; Le Rouzic, E.; Morel, M.; Collin, G.; Descamps, D.; Damond, F.; Brun-Vezinet, F.; Nisole, S.; et al. The human immunodeficiency virus type 2 vpx protein usurps the cul4a-ddb1 dcaf1 ubiquitin ligase to overcome a postentry block in macrophage infection. J. Virol. 2009, 83, 4854–4860. [Google Scholar]
  151. Hrecka, K.; Hao, C.; Gierszewska, M.; Swanson, S.K.; Kesik-Brodacka, M.; Srivastava, S.; Florens, L.; Washburn, M.P.; Skowronski, J. Vpx relieves inhibition of hiv-1 infection of macrophages mediated by the samhd1 protein. Nature 2011, 474, 658–661. [Google Scholar]
  152. Laguette, N.; Sobhian, B.; Casartelli, N.; Ringeard, M.; Chable-Bessia, C.; Segeral, E.; Yatim, A.; Emiliani, S.; Schwartz, O.; Benkirane, M. Samhd1 is the dendritic- and myeloid-cell-specific hiv-1 restriction factor counteracted by vpx. Nature 2011, 474, 654–657. [Google Scholar]
  153. Rice, G.I.; Bond, J.; Asipu, A.; Brunette, R.L.; Manfield, I.W.; Carr, I.M.; Fuller, J.C.; Jackson, R.M.; Lamb, T.; Briggs, T.A.; et al. Mutations involved in aicardi-goutieres syndrome implicate samhd1 as regulator of the innate immune response. Nat. Genet. 2009, 41, 829–832. [Google Scholar]
  154. Crow, Y.J.; Leitch, A.; Hayward, B.E.; Garner, A.; Parmar, R.; Griffith, E.; Ali, M.; Semple, C.; Aicardi, J.; Babul-Hirji, R.; et al. Mutations in genes encoding ribonuclease h2 subunits cause aicardi-goutieres syndrome and mimic congenital viral brain infection. Nat. Genet. 2006, 38, 910–916. [Google Scholar]
  155. Crow, Y.J.; Hayward, B.E.; Parmar, R.; Robins, P.; Leitch, A.; Ali, M.; Black, D.N.; van Bokhoven, H.; Brunner, H.G.; Hamel, B.C.; et al. Mutations in the gene encoding the 3'-5' DNA exonuclease trex1 cause aicardi-goutieres syndrome at the ags1 locus. Nat. Genet. 2006, 38, 917–920. [Google Scholar]
  156. Yan, N.; Regalado-Magdos, A.D.; Stiggelbout, B.; Lee-Kirsch, M.A.; Lieberman, J. The cytosolic exonuclease trex1 inhibits the innate immune response to human immunodeficiency virus type 1. Nat. Immunol. 2010, 11, 1005–1013. [Google Scholar] [CrossRef]
  157. Genovesio, A.; Kwon, Y.J.; Windisch, M.P.; Kim, N.Y.; Choi, S.Y.; Kim, H.C.; Jung, S.; Mammano, F.; Perrin, V.; Boese, A.S.; et al. Automated genome-wide visual profiling of cellular proteins involved in hiv infection. J. Biomol. Screening 2011, 16, 945–958. [Google Scholar] [CrossRef]
  158. Goldstone, D.C.; Ennis-Adeniran, V.; Hedden, J.J.; Groom, H.C.; Rice, G.I.; Christodoulou, E.; Walker, P.A.; Kelly, G.; Haire, L.F.; Yap, M.W.; et al. Hiv-1 restriction factor samhd1 is a deoxynucleoside triphosphate triphosphohydrolase. Nature 2011, 480, 379–382. [Google Scholar]
  159. Powell, R.D.; Holland, P.J.; Hollis, T.; Perrino, F.W. Aicardi-goutieres syndrome gene and hiv-1 restriction factor samhd1 is a dgtp-regulated deoxynucleotide triphosphohydrolase. J. Biol. Chem. 2011, 286, 43596–43600. [Google Scholar]
  160. Lahouassa, H.; Daddacha, W.; Hofmann, H.; Ayinde, D.; Logue, E.C.; Dragin, L.; Bloch, N.; Maudet, C.; Bertrand, M.; Gramberg, T.; et al. Samhd1 restricts the replication of human immunodeficiency virus type 1 by depleting the intracellular pool of deoxynucleoside triphosphates. Nat. Immunol. 2012, 13, 223–228. [Google Scholar]
  161. Manel, N.; Hogstad, B.; Wang, Y.; Levy, D.E.; Unutmaz, D.; Littman, D.R. A cryptic sensor for hiv-1 activates antiviral innate immunity in dendritic cells. Nature 2010, 467, 214–217. [Google Scholar]
  162. Beignon, A.S.; McKenna, K.; Skoberne, M.; Manches, O.; DaSilva, I.; Kavanagh, D.G.; Larsson, M.; Gorelick, R.J.; Lifson, J.D.; Bhardwaj, N. Endocytosis of hiv-1 activates plasmacytoid dendritic cells via toll-like receptor-viral rna interactions. J. Clin. Invest. 2005, 115, 3265–3275. [Google Scholar]
  163. Tsang, J.; Chain, B.M.; Miller, R.F.; Webb, B.L.; Barclay, W.; Towers, G.J.; Katz, D.R.; Noursadeghi, M. Hiv-1 infection of macrophages is dependent on evasion of innate immune cellular activation. AIDS 2009, 23, 2255–2263. [Google Scholar] [CrossRef]
  164. Asaoka, K.; Ikeda, K.; Hishinuma, T.; Horie-Inoue, K.; Takeda, S.; Inoue, S. A retrovirus restriction factor trim5alpha is transcriptionally regulated by interferons. Biochem. Biophys. Res. Commun. 2005, 338, 1950–1956. [Google Scholar] [CrossRef]
  165. Tanaka, Y.; Marusawa, H.; Seno, H.; Matsumoto, Y.; Ueda, Y.; Kodama, Y.; Endo, Y.; Yamauchi, J.; Matsumoto, T.; Takaori-Kondo, A.; et al. Anti-viral protein apobec3g is induced by interferon-alpha stimulation in human hepatocytes. Biochem. Biophys. Res. Commun. 2006, 341, 314–319. [Google Scholar] [CrossRef]
  166. Carthagena, L.; Parise, M.C.; Ringeard, M.; Chelbi-Alix, M.K.; Hazan, U.; Nisole, S. Implication of trim alpha and trimcyp in interferon-induced anti-retroviral restriction activities. Retrovirology 2008, 5, 59. [Google Scholar] [CrossRef]
  167. Tissot, C.; Mechti, N. Molecular cloning of a new interferon-induced factor that represses human immunodeficiency virus type 1 long terminal repeat expression. J. Biol. Chem. 1995, 270, 14891–14898. [Google Scholar]
  168. Kajaste-Rudnitski, A.; Marelli, S.S.; Pultrone, C.; Pertel, T.; Uchil, P.D.; Mechti, N.; Mothes, W.; Poli, G.; Luban, J.; Vicenzi, E. Trim22 inhibits hiv-1 transcription independently of its e3 ubiquitin ligase activity, tat, and nf-kappab-responsive long terminal repeat elements. J. Virol. 2011, 85, 5183–5196. [Google Scholar]
  169. Barr, S.D.; Smiley, J.R.; Bushman, F.D. The interferon response inhibits hiv particle production by induction of trim22. PLoS Pathog. 2008, 4, e1000007. [Google Scholar] [CrossRef]
  170. Lu, J.; Pan, Q.; Rong, L.; He, W.; Liu, S.L.; Liang, C. The ifitm proteins inhibit hiv-1 infection. J. Virol. 2011, 85, 2126–2137. [Google Scholar]
  171. Poli, G.; Orenstein, J.M.; Kinter, A.; Folks, T.M.; Fauci, A.S. Interferon-alpha but not azt suppresses hiv expression in chronically infected cell lines. Science 1989, 244, 575–577. [Google Scholar]
  172. Bednarik, D.P.; Mosca, J.D.; Raj, N.B.; Pitha, P.M. Inhibition of human immunodeficiency virus (hiv) replication by hiv-trans-activated alpha 2-interferon. Proc. Natl. Acad. Sci. USA 1989, 86, 4958–4962. [Google Scholar]
  173. Meylan, P.R.; Guatelli, J.C.; Munis, J.R.; Richman, D.D.; Kornbluth, R.S. Mechanisms for the inhibition of hiv replication by interferons-alpha, -beta, and -gamma in primary human macrophages. Virology 1993, 193, 138–148. [Google Scholar] [CrossRef]
  174. Gendelman, H.E.; Baca, L.M.; Turpin, J.; Kalter, D.C.; Hansen, B.; Orenstein, J.M.; Dieffenbach, C.W.; Friedman, R.M.; Meltzer, M.S. Regulation of hiv replication in infected monocytes by ifn-alpha. Mechanisms for viral restriction. J. Immunol. 1990, 145, 2669–2676. [Google Scholar]
  175. Shirazi, Y.; Pitha, P.M. Interferon alpha-mediated inhibition of human immunodeficiency virus type 1 provirus synthesis in t-cells. Virology 1993, 193, 303–312. [Google Scholar] [CrossRef]
  176. Agy, M.B.; Acker, R.L.; Sherbert, C.H.; Katze, M.G. Interferon treatment inhibits virus replication in hiv-1- and siv-infected cd4+ t-cell lines by distinct mechanisms: Evidence for decreased stability and aberrant processing of hiv-1 proteins. Virology 1995, 214, 379–386. [Google Scholar] [CrossRef]
  177. Goujon, C.; Malim, M.H. Characterization of the alpha interferon-induced postentry block to hiv-1 infection in primary human macrophages and t cells. J. Virol. 2010, 84, 9254–9266. [Google Scholar] [CrossRef]
  178. Hatziioannou, T.; Princiotta, M.; Piatak, M., Jr.; Yuan, F.; Zhang, F.; Lifson, J.D.; Bieniasz, P.D. Generation of simian-tropic hiv-1 by restriction factor evasion. Science 2006, 314, 95. [Google Scholar]
  179. Kamada, K.; Igarashi, T.; Martin, M.A.; Khamsri, B.; Hatcho, K.; Yamashita, T.; Fujita, M.; Uchiyama, T.; Adachi, A. Generation of hiv-1 derivatives that productively infect macaque monkey lymphoid cells. Proc. Natl. Acad. Sci. USA 2006, 103, 16959–16964. [Google Scholar]
  180. Igarashi, T.; Iyengar, R.; Byrum, R.A.; Buckler-White, A.; Dewar, R.L.; Buckler, C.E.; Lane, H.C.; Kamada, K.; Adachi, A.; Martin, M.A. Human immunodeficiency virus type 1 derivative with 7% simian immunodeficiency virus genetic content is able to establish infections in pig-tailed macaques. J. Virol. 2007, 81, 11549–11552. [Google Scholar]
  181. Kuroishi, A.; Saito, A.; Shingai, Y.; Shioda, T.; Nomaguchi, M.; Adachi, A.; Akari, H.; Nakayama, E.E. Modification of a loop sequence between alpha-helices 6 and 7 of virus capsid (ca) protein in a human immunodeficiency virus type 1 (hiv-1) derivative that has simian immunodeficiency virus (sivmac239) vif and ca alpha-helices 4 and 5 loop improves replication in cynomolgus monkey cells. Retrovirology 2009, 6, 70. [Google Scholar]
  182. Saito, A.; Nomaguchi, M.; Iijima, S.; Kuroishi, A.; Yoshida, T.; Lee, Y.-J.; Hayakawa, T.; Kono, K.; Nakayama, E.E.; Shioda, T.; et al. Improved capacity of a monkey-tropic hiv-1 derivative to replicate in cynomolgus monkeys with minimal modifications. Microbes Infection 2011, 13, 58–64. [Google Scholar]
  183. Agy, M.B.; Frumkin, L.R.; Corey, L.; Coombs, R.W.; Wolinsky, S.M.; Koehler, J.; Morton, W.R.; Katze, M.G. Infection of macaca nemestrina by human immunodeficiency virus type-1. Science 1992, 257, 103–106. [Google Scholar]
  184. Agy, M.B.; Schmidt, A.; Florey, M.J.; Kennedy, B.J.; Schaefer, G.; Katze, M.G.; Corey, L.; Morton, W.R.; Bosch, M.L. Serial in vivo passage of hiv-1 infection in macaca nemestrina. Virology 1997, 238, 336–343. [Google Scholar] [CrossRef]
  185. Gartner, S.; Liu, Y.; Lewis, M.G.; Polonis, V.; Elkins, W.R.; Zack, P.M.; Miao, J.; Hunter, E.A.; Greenhouse, J.; Eddy, G.A. Hiv-1 infection in pigtailed macaques. AIDS Res. Hum. Retroviruses 1994, 10 Suppl 2, S129–133. [Google Scholar]
  186. Frumkin, L.R.; Agy, M.B.; Coombs, R.W.; Panther, L.; Morton, W.R.; Koehler, J.; Florey, M.J.; Dragavon, J.; Schmidt, A.; Katze, M.G.; et al. Acute infection of macaca nemestrina by human immunodeficiency virus type 1. Virology 1993, 195, 422–431. [Google Scholar] [CrossRef]
  187. Gartner, S.; Liu, Y.; Polonis, V.; Lewis, M.G.; Elkins, W.R.; Hunter, E.A.; Miao, J.; Corts, K.J.; Eddy, G.A. Adaptation of hiv-1 to pigtailed macaques. J. Med. Primatology 1994, 23, 155–163. [Google Scholar] [CrossRef]
  188. Bosch, M.L.; Schmidt, A.; Chen, J.; Florey, M.J.; Agy, M.; Morton, W.R. Enhanced replication of hiv-1 in vivo in pigtailed macaques (macaca nemestrina). J. Med. Primatology 2000, 29, 107–113. [Google Scholar]
  189. Bosch, M.L.; Schmidt, A.; Agy, M.B.; Kimball, L.E.; Morton, W.R. Infection of macaca nemestrina neonates with hiv-1 via different routes of inoculation. AIDS 1997, 11, 1555–1563. [Google Scholar] [CrossRef]
  190. Thippeshappa, R.; Polacino, P.; Yu Kimata, M.T.; Siwak, E.B.; Anderson, D.; Wang, W.; Sherwood, L.; Arora, R.; Wen, M.; Zhou, P.; et al. Vif substitution enables persistent infection of pig-tailed macaques by human immunodeficiency virus type 1. J. Virol. 2011, 85, 3767–3779. [Google Scholar]
  191. Brennan, G.; Kozyrev, Y.; Kodama, T.; Hu, S.L. Novel trim5 isoforms expressed by macaca nemestrina. J. Virol. 2007, 81, 12210–12217. [Google Scholar]
  192. Liao, C.H.; Kuang, Y.Q.; Liu, H.L.; Zheng, Y.T.; Su, B. A novel fusion gene, trim5-cyclophilin a in the pig-tailed macaque determines its susceptibility to hiv-1 infection. AIDS 2007, 21, S19–S26. [Google Scholar]
  193. Newman, R.M.; Hall, L.; Kirmaier, A.; Pozzi, L.A.; Pery, E.; Farzan, M.; O'Neil, S.P.; Johnson, W. Evolution of a trim5-cypa splice isoform in old world monkeys. PLoS Pathog. 2008, 4, e1000003. [Google Scholar] [CrossRef]
  194. Hatziioannou, T.; Ambrose, Z.; Chung, N.P.; Piatak, M., Jr.; Yuan, F.; Trubey, C.M.; Coalter, V.; Kiser, R.; Schneider, D.; Smedley, J.; et al. A macaque model of hiv-1 infection. Proc. Natl. Acad. Sci. USA 2009, 106, 4425–4429. [Google Scholar]
  195. Kimata, J.T.; Kuller, L.; Anderson, D.B.; Dailey, P.; Overbaugh, J. Emerging cytopathic and antigenic simian immunodeficiency virus variants influence aids progression. Nat.Med. 1999, 5, 535–541. [Google Scholar] [CrossRef]
  196. Kimata, J.T.; Mozaffarian, A.; Overbaugh, J. A lymph node-derived cytopathic simian immunodeficiency virus mne variant replicates in nonstimulated peripheral blood mononuclear cells. J. Virol. 1998, 72, 245–256. [Google Scholar]
  197. Thippeshappa, R.; Kimata, J.T. Unpublished Work. Baylor College of Medicine: Houston, Texas, USA, 2012. [Google Scholar]
  198. Virgen, C.A.; Hatziioannou, T. Antiretroviral activity and vif sensitivity of rhesus macaque apobec3 proteins. J. Virol. 2007, 81, 13932–13937. [Google Scholar]
  199. Chen, K.; Huang, J.; Zhang, C.; Huang, S.; Nunnari, G.; Wang, F.X.; Tong, X.; Gao, L.; Nikisher, K.; Zhang, H. Alpha interferon potently enhances the anti-human immunodeficiency virus type 1 activity of apobec3g in resting primary cd4 t cells. J. Virol. 2006, 80, 7645–7657. [Google Scholar]
  200. Koning, F.A.; Newman, E.N.; Kim, E.Y.; Kunstman, K.J.; Wolinsky, S.M.; Malim, M.H. Defining apobec3 expression patterns in human tissues and hematopoietic cell subsets. J. Virol. 2009, 83, 9474–9485. [Google Scholar]
  201. Pido-Lopez, J.; Whittall, T.; Wang, Y.; Bergmeier, L.A.; Babaahmady, K.; Singh, M.; Lehner, T. Stimulation of cell surface ccr5 and cd40 molecules by their ligands or by hsp70 up-regulates apobec3g expression in cd4(+) t cells and dendritic cells. J. Immunol. 2007, 178, 1671–1679. [Google Scholar]
  202. Refsland, E.W.; Stenglein, M.D.; Shindo, K.; Albin, J.S.; Brown, W.L.; Harris, R.S. Quantitative profiling of the full apobec3 mrna repertoire in lymphocytes and tissues: Implications for hiv-1 restriction. Nucleic Acids Res. 2010, 38, 4274–4284. [Google Scholar]
  203. Rose, K.M.; Marin, M.; Kozak, S.L.; Kabat, D. Transcriptional regulation of apobec3g, a cytidine deaminase that hypermutates human immunodeficiency virus. J. Biol. Chem. 2004, 279, 41744–41749. [Google Scholar]
  204. Sarkis, P.T.; Ying, S.; Xu, R.; Yu, X.F. Stat1-independent cell type-specific regulation of antiviral apobec3g by ifn-alpha. J. Immunol. 2006, 177, 4530–4540. [Google Scholar]
  205. Stopak, K.S.; Chiu, Y.L.; Kropp, J.; Grant, R.M.; Greene, W.C. Distinct patterns of cytokine regulation of apobec3g expression and activity in primary lymphocytes, macrophages, and dendritic cell. J. Biol. Chem. 2007, 282, 3539–3546. [Google Scholar]
  206. Ying, S.; Zhang, X.; Sarkis, P.T.; Xu, R.; Yu, X. Cell-specific regulation of apobec3f by interferons. Acta Biochim. Biophy. Sin. 2007, 39, 297–304. [Google Scholar] [CrossRef]
  207. Dettenhofer, M.; Yu, X.F. Highly purified human immunodeficiency virus type 1 reveals a virtual absence of vif in virions. J. Virol. 1999, 73, 1460–1467. [Google Scholar]
  208. Kao, S.; Akari, H.; Khan, M.A.; Dettenhofer, M.; Yu, X.F.; Strebel, K. Human immunodeficiency virus type 1 vif is efficiently packaged into virions during productive but not chronic infection. J. Virol. 2003, 77, 1131–1140. [Google Scholar] [CrossRef]
  209. Khan, M.A.; Aberham, C.; Kao, S.; Akari, H.; Gorelick, R.; Bour, S.; Strebel, K. Human immunodeficiency virus type 1 vif protein is packaged into the nucleoprotein complex through an interaction with viral genomic rna. J. Virol. 2001, 75, 7252–7265. [Google Scholar]
  210. Liu, B.; Yu, X.; Luo, K.; Yu, Y.; Yu, X.F. Influence of primate lentiviral vif and proteasome inhibitors on human immunodeficiency virus type 1 virion packaging of apobec3g. J. Virol. 2004, 78, 2072–2081. [Google Scholar] [CrossRef]
  211. Liu, H.; Wu, X.; Newman, M.; Shaw, G.M.; Hahn, B.H.; Kappes, J.C. The vif protein of human and simian immunodeficiency viruses is packaged into virions and associates with viral core structures. J. Virol. 1995, 69, 7630–7638. [Google Scholar]
  212. Simon, J.H.; Miller, D.L.; Fouchier, R.A.; Malim, M.H. Virion incorporation of human immunodeficiency virus type-1 vif is determined by intracellular expression level and may not be necessary for function. Virology 1998, 248, 182–187. [Google Scholar] [CrossRef]
  213. Carr, J.M.; Coolen, C.; Davis, A.J.; Burrell, C.J.; Li, P. Human immunodeficiency virus 1 (hiv-1) virion infectivity factor (vif) is part of reverse transcription complexes and acts as an accessory factor for reverse transcription. Virology 2008, 372, 147–156. [Google Scholar] [CrossRef]
  214. Izumi, T.; Io, K.; Matsui, M.; Shirakawa, K.; Shinohara, M.; Nagai, Y.; Kawahara, M.; Kobayashi, M.; Kondoh, H.; Misawa, N.; et al. Hiv-1 viral infectivity factor interacts with tp53 to induce g2 cell cycle arrest and positively regulate viral replication. Proc. Natl. Acad. Sci. USA 2010, 107, 20798–20803. [Google Scholar]
  215. Okumura, A.; Alce, T.; Lubyova, B.; Ezelle, H.; Strebel, K.; Pitha, P.M. Hiv-1 accessory proteins vpr and vif modulate antiviral response by targeting irf-3 for degradation. Virology 2008, 373, 85–97. [Google Scholar] [CrossRef]
  216. Sakai, K.; Dimas, J.; Lenardo, M.J. The vif and vpr accessory proteins independently cause Hiv-1-induced t cell cytopathicity and cell cycle arrest. Proc. Natl. Acad. Sci. USA 2006, 103, 3369–3374. [Google Scholar]
  217. Alexander, L.; Du, Z.; Howe, A.Y.; Czajak, S.; Desrosiers, R.C. Induction of aids in rhesus monkeys by a recombinant simian immunodeficiency virus expressing nef of human immunodeficiency virus type 1. J. Virol. 1999, 73, 5814–5825. [Google Scholar]
  218. Kirchhoff, F.; Munch, J.; Carl, S.; Stolte, N.; Matz-Rensing, K.; Fuchs, D.; Haaft, P.T.; Heeney, J.L.; Swigut, T.; Skowronski, J.; et al. The human immunodeficiency virus type 1 nef gene can to a large extent replace simian immunodeficiency virus nef in vivo. J. Virol. 1999, 73, 8371–8383. [Google Scholar]
  219. Mandell, C.P.; Reyes, R.A.; Cho, K.; Sawai, E.T.; Fang, A.L.; Schmidt, K.A.; Luciw, P.A. Siv/hiv nef recombinant virus (shivnef) produces simian aids in rhesus macaques. Virology 1999, 265, 235–251. [Google Scholar] [CrossRef]
  220. Thippeshappa, R.P.P.P.; Yu-Kimata, M.T.; Anderson, D.; Wang, W.; Arora, R.; Wen, M.; Zhou, P.; Hu, S.-L.; Kimata, J.T. Overcoming Restriction by Apobec3 Family Protiens Is Key for Persistent Hiv-1 Infection of Pig-Tailed Macaques. In Proceeding of 30th Annual Meeting of the American Society for Virology, University of Minnesota, Minneapolis, MN, USA, 16-20 July 2011.
  221. Thippeshappa, R.P.P.; Ruan, H.; Yu-Kimata, M.T.; Siwak, E.B.; Anderson, D.; Wang, W.; Arora, R.; Wen, M.; Zhou, P.; Hu, S-L.; Kimata, J.T. Pig-tail tropic human immunodeficiency virus type 1 is susceptible to innate immune factors compared to pathogenic sivmne. In Proceeding of 29th Annual Symposium on Nonhuman Primate Models for AIDS Seattle, Washington, DC, USA, 28 October 2011.

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Thippeshappa, R.; Ruan, H.; Kimata, J.T. Breaking Barriers to an AIDS Model with Macaque-Tropic HIV-1 Derivatives. Biology 2012, 1, 134-164. https://doi.org/10.3390/biology1020134

AMA Style

Thippeshappa R, Ruan H, Kimata JT. Breaking Barriers to an AIDS Model with Macaque-Tropic HIV-1 Derivatives. Biology. 2012; 1(2):134-164. https://doi.org/10.3390/biology1020134

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Thippeshappa, Rajesh, Hongmei Ruan, and Jason T. Kimata. 2012. "Breaking Barriers to an AIDS Model with Macaque-Tropic HIV-1 Derivatives" Biology 1, no. 2: 134-164. https://doi.org/10.3390/biology1020134

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