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Metabolites 2011, 1(1), 21-40; doi:10.3390/metabo1010021

Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry — Meets a Key Challenge in Lipidomics

1
Division of Bioorganic Chemistry and Molecular Pharmacology, Department of Internal Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA
2
Sanford-Burnham Medical Research Institute, Orlando, FL 32827, USA
*
Author to whom correspondence should be addressed.
Received: 8 October 2011 / Revised: 2 November 2011 / Accepted: 4 November 2011 / Published: 11 November 2011
(This article belongs to the Special Issue Lipidomics)
View Full-Text   |   Download PDF [223 KB, uploaded 11 November 2011]   |  

Abstract

Electrospray ionization mass spectrometry (ESI-MS) has become one of the most popular and powerful technologies to identify and quantify individual lipid species in lipidomics. Meanwhile, quantitative analysis of lipid species by ESI-MS has also become a major obstacle to meet the challenges of lipidomics. Herein, we discuss the principles, advantages, and possible limitations of different mass spectrometry-based methodologies for lipid quantification, as well as a few practical issues important for accurate quantification of individual lipid species. Accordingly, accurate quantification of individual lipid species, one of the key challenges in lipidomics, can be practically met. View Full-Text
Keywords: electrospray ionization mass spectrometry; lipidomics; quantification; shotgun lipidomics electrospray ionization mass spectrometry; lipidomics; quantification; shotgun lipidomics
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This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Yang, K.; Han, X. Accurate Quantification of Lipid Species by Electrospray Ionization Mass Spectrometry — Meets a Key Challenge in Lipidomics. Metabolites 2011, 1, 21-40.

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