Next Article in Journal / Special Issue
Metabolic Changes in Synechocystis PCC6803 upon Nitrogen-Starvation: Excess NADPH Sustains Polyhydroxybutyrate Accumulation
Previous Article in Journal
Characterisation of the Metabolites of 1,8-Cineole Transferred into Human Milk: Concentrations and Ratio of Enantiomers
Metabolites 2013, 3(1), 72-100; doi:10.3390/metabo3010072

Recent Applications of Metabolomics Toward Cyanobacteria

1 Institut Biowissenschaften, Pflanzenphysiologie, Universität Rostock, Albert-Einstein-Str. 3, D-18059 Rostock, Germany 2 Max-Planck-Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg 1, 14476 Golm, Germany
* Author to whom correspondence should be addressed.
Received: 20 December 2012 / Revised: 23 January 2013 / Accepted: 23 January 2013 / Published: 4 February 2013
(This article belongs to the Special Issue Metabolism in Phototrophic Prokaryotes and Algae)
View Full-Text   |   Download PDF [1291 KB, uploaded 4 February 2013]   |   Browse Figures


Our knowledge on cyanobacterial molecular biology increased tremendously by the application of the “omics” techniques. Only recently, metabolomics was applied systematically to model cyanobacteria. Metabolomics, the quantitative estimation of ideally the complete set of cellular metabolites, is particularly well suited to mirror cellular metabolism and its flexibility under diverse conditions. Traditionally, small sets of metabolites are quantified in targeted metabolome approaches. The development of separation technologies coupled to mass-spectroscopy- or nuclear-magnetic-resonance-based identification of low molecular mass molecules presently allows the profiling of hundreds of metabolites of diverse chemical nature. Metabolome analysis was applied to characterize changes in the cyanobacterial primary metabolism under diverse environmental conditions or in defined mutants. The resulting lists of metabolites and their steady state concentrations in combination with transcriptomics can be used in system biology approaches. The application of stable isotopes in fluxomics, i.e. the quantitative estimation of carbon and nitrogen fluxes through the biochemical network, has only rarely been applied to cyanobacteria, but particularly this technique will allow the making of kinetic models of cyanobacterial systems. The further application of metabolomics in the concert of other “omics” technologies will not only broaden our knowledge, but will also certainly strengthen the base for the biotechnological application of cyanobacteria.
Keywords: inorganic carbon; glucose feeding; modeling; mutant; primary metabolism; stable isotope labeling; synechocystis inorganic carbon; glucose feeding; modeling; mutant; primary metabolism; stable isotope labeling; synechocystis
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Schwarz, D.; Orf, I.; Kopka, J.; Hagemann, M. Recent Applications of Metabolomics Toward Cyanobacteria. Metabolites 2013, 3, 72-100.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Metabolites EISSN 2218-1989 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert