Table of Contents
Biomedicines, Volume 5, Issue 4 (December 2017)
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Cover Story (view full-size image) This confocal image shows a vessel in green (Flk1-GFP transgenic mice) coming from a muscle and [...] Read more. This confocal image shows a vessel in green (Flk1-GFP transgenic mice) coming from a muscle and entering a cortical bone. Osteocytes appear in red within the bone due to a Dextran Texas Red lysine fixable (10,000 MW) injection, 5 min before the sacrifice (Kamel‐ElSayed SA, et al. Bone. 2015), and all the cells are stained in blue with DAPI to show the nucleus (1:2000). The top of this image shows bone marrow. Briefly, the femur was fixed in 4% PFA for 48 h at +4 °C and demineralized in EDTA 10% for 1 week. It was then bathed in sucrose 15% and 30% before being embedded in OCT. A 50 µm cryosection was cut using a cryostat. This image has been realized by Dr. Delphine Maurel and Dr. Claudine Boiziau. View this paper