Quantifying the Effects of Frequency and Amplitude of Periodic Oxygen-Related Stress on Recombinant Protein Production in Pichia pastoris
Abstract
:1. Introduction
2. Materials and Methods
2.1. Biological Model System
2.2. Experimental Setup and Approach
(a) | ||
Exp. No. | Residence time | Dissolved oxygen |
1 | Low (3.3 min) | Intermediate (25%) |
2 | High (15 min) | Intermediate (25%) |
3 | Low (3.3 min) | High (70%) |
4 | High (15 min) | High (70%) |
5 | Low (3.3 min) | Intermediate (25%) |
(b) | ||
6 | Low (3.3 min) | Low (5%) |
7 | 0 min | Low (5%) |
(c) | ||
1 | (3.3 min) | (25%) |
5 | (3.3 min) | (25%) |
6 | (3.3 min) | (5%) |
7 | 0 | (5%) |
8 | 0 | (25%) |
9 | 0 | (70%) |
10 | (3.3 min) | (70%) |
11 | (15 min) | (5%) |
12 | (15min) | (25%) |
13 | (15 min) | (70%) |
2.3. Analytics
2.4. Data Treatment
3. Results and Discussion
3.1. Time Course of Dissolved Oxygen Level
3.2. Results DOE1
3.3. Along Which Function is Productivity Coupled to Dissolved Oxygen?
4. Conclusions
Supplementary Files
Supplementary File 1Conflicts of Interest
References
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Appendix
Exp. No. | Residence time (min) | Dissolved oxygen (%) | Specific productivity (U/g·h) | Titer(U) | Y_CO2/s (C-mol/C-mol) | Y_x/s (C-mol/C-mol) |
---|---|---|---|---|---|---|
1 | Low (3.3) | Intermediate (25) | 4.95 ± 0.24 | 16,198.2 ± 95 | 0.58 ± 0.021 | 0.30 ± 0.012 |
2 | High (15) | Intermediate (25) | 3.98 ± 0.19 | 20,665.1 ± 110 | 0.63 ± 0.022 | 0.29 ± 0.011 |
3 | Low (3.3) | High (70) | 2.87 ± 0.14 | 10,915.4 ± 50 | 0.65 ± 0.022 | 0.28 ± 0.011 |
4 | High (15) | High (70) | 2.19 ± 0.10 | 10,048.3 ± 48 | 0.64 ± 0.03 | 0.28 ± 0.011 |
5 | Low (3.3) | Intermediate (25) | 4.41 ± 0.22 | 18,482.8 ± 85 | 0.59 ± 0.021 | 0.29 ± 0.011 |
Exp. No | Residence time (min) | Dissolved oxygen (%) | Specific productivity (U/g·h) |
---|---|---|---|
1 | 3.3 | 25 | 4.95 ± 0.24 |
5 | 3.3 | 25 | 4.41 ± 0.22 |
6 | 3.3 | 5 | 2.25 ± 0.22 |
7 | 0 | 5 | 2.80 ± 0.28 |
8 | 0 | 25 | 4.60 ± 0.30 |
9 | 0 | 70 | 2.60 ± 0.31 |
10 | 3.3 | 70 | 2.20 ± 0.21 |
11 | 15 | 5 | 2.18 ± 0.20 |
12 | 15 | 25 | 2.33 ± 0.22 |
13 | 15 | 70 | 2.08 ± 0.19 |
Description of the Two-Compartment System
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Jazini, M.; Cekici, G.; Herwig, C. Quantifying the Effects of Frequency and Amplitude of Periodic Oxygen-Related Stress on Recombinant Protein Production in Pichia pastoris. Bioengineering 2014, 1, 47-61. https://doi.org/10.3390/bioengineering1010047
Jazini M, Cekici G, Herwig C. Quantifying the Effects of Frequency and Amplitude of Periodic Oxygen-Related Stress on Recombinant Protein Production in Pichia pastoris. Bioengineering. 2014; 1(1):47-61. https://doi.org/10.3390/bioengineering1010047
Chicago/Turabian StyleJazini, Mohammadhadi, Gülbahar Cekici, and Christoph Herwig. 2014. "Quantifying the Effects of Frequency and Amplitude of Periodic Oxygen-Related Stress on Recombinant Protein Production in Pichia pastoris" Bioengineering 1, no. 1: 47-61. https://doi.org/10.3390/bioengineering1010047