Applications of Digital Holographic Microscopy

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Optics and Lasers".

Deadline for manuscript submissions: closed (31 March 2018) | Viewed by 33555

Special Issue Editors

Univ Stuttgart, Inst Tech Opt, Pfaffenwaldring 9, D-70569 Stuttgart, Germany
Universität Stuttgart, Institut für Technische Optik, Pfaffenwaldring 9, 70569 Stuttgart, Deutschland
Interests: digital holography; phase retrieval imaging; ptychography; light field imaging; elastography; optomechanical design
Univ Stuttgart, Inst Tech Opt, Pfaffenwaldring 9, D-70569 Stuttgart, Germany

Special Issue Information

Dear Colleagues,

We jointly invite you to submit a paper for this special issue dedicated to digital holographic microscopy and its applications. The scope of this issue likewise embraces related techniques, such as lensless digital holography, in-line digital holography, terahertz digital holography, phase retrieval imaging, and phase contrast imaging techniques. The main core of this call relates to the recovery of high-resolution quantitative phase imaging, with applications ranging from optical surface metrology, biomedical imaging, resolution enhancement and measurement uncertainty improving techniques. Moreover, hybrid or multimodal quantitative phase imaging techniques also fall within this call, including the combination of quantitative phase imaging with tomography (as in optical diffraction tomography), and multiple wavelength quantitative phase imaging. Novel numerical tools to recover or process the quantitative phase imaging will form a further part of this issue.

We hope that you find the content of this call relevant for your research and will consider publication of your work within this special issue.

Kind regards,

Prof. Dr. Osten Wolfgang
Dr. Daniel Claus
Dr. Giancarlo Pedrini
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • digital holographic microscopy
  • quantitative phase imaging
  • phase retrieval imaging
  • ptychography
  • lensless imaging

Published Papers (7 papers)

Order results
Result details
Select all
Export citation of selected articles as:

Research

Jump to: Review

10 pages, 2963 KiB  
Article
Variable Wavefront Curvature Phase Retrieval Compared to Off-Axis Holography and Its Useful Application to Support Intraoperative Tissue Discrimination
by Daniel Claus, Jörg Hennenlotter, Qi Liting, Giancarlo Pedrini, Arnulf Stenzl and Wolfgang Osten
Appl. Sci. 2018, 8(11), 2147; https://doi.org/10.3390/app8112147 - 03 Nov 2018
Cited by 2 | Viewed by 2239
Abstract
Quantitative phase imaging can reveal morphological features without having to stain the biological sample. This property has important implications for intraoperative applications since the time spent during histopathology can be reduced from a few minutes to a few seconds. However, most common quantitative [...] Read more.
Quantitative phase imaging can reveal morphological features without having to stain the biological sample. This property has important implications for intraoperative applications since the time spent during histopathology can be reduced from a few minutes to a few seconds. However, most common quantitative phase imaging techniques are based on the interferometric principle, which makes them more prone to disturbing environmental influences, such as temperature drift and air turbulence. In the last decade, with the advance of computing power, many different iterative quantitative phase imaging techniques, which only require the recording of the diffracted wavefield, and therefore offer increased robustness towards environmental disturbances, have been proposed. These are particularly well-suited for the application outside the well-controlled lab environment such as an operating theatre. The optical performance of our developed iterative phase retrieval method based on variable wavefront curvature will be evaluated by reference to off-axis digital holography and applied for intraoperative discrimination of tissue. Full article
(This article belongs to the Special Issue Applications of Digital Holographic Microscopy)
Show Figures

Figure 1

14 pages, 2717 KiB  
Article
Optogenetic Stimulation of Human Neural Networks Using Fast Ferroelectric Spatial Light Modulator—Based Holographic Illumination
by Felix Schmieder, Simon D. Klapper, Nektarios Koukourakis, Volker Busskamp and Jürgen W. Czarske
Appl. Sci. 2018, 8(7), 1180; https://doi.org/10.3390/app8071180 - 19 Jul 2018
Cited by 33 | Viewed by 5451
Abstract
The generation and application of human stem-cell-derived functional neural circuits promises novel insights into neurodegenerative diseases. These networks are often studied using stem-cell derived random neural networks in vitro, with electrical stimulation and recording using multielectrode arrays. However, the impulse response function of [...] Read more.
The generation and application of human stem-cell-derived functional neural circuits promises novel insights into neurodegenerative diseases. These networks are often studied using stem-cell derived random neural networks in vitro, with electrical stimulation and recording using multielectrode arrays. However, the impulse response function of networks is best obtained with spatiotemporally well-defined stimuli, which electrical stimulation does not provide. Optogenetics allows for the functional control of genetically altered cells with light stimuli at high spatiotemporal resolution. Current optogenetic investigations of neural networks are often conducted using full field illumination, potentially masking important functional information. This can be avoided using holographically shaped illumination. In this article, we present a digital holographic illumination setup with a spatial resolution of about 8 µm, which suffices for the stimulation of single neurons, and offers a temporal resolution of less than 0.6 ms. With this setup, we present preliminary single-cell stimulation recording of stem-cell derived induced human neurons in a random neural network. This will offer the opportunity for further studies on connectivity in such networks. Full article
(This article belongs to the Special Issue Applications of Digital Holographic Microscopy)
Show Figures

Figure 1

13 pages, 6614 KiB  
Article
Extending the Depth of Field beyond Geometrical Imaging Limitations Using Phase Noise as a Focus Measure in Multiwavelength Digital Holography
by Tobias Seyler, Markus Fratz, Tobias Beckmann, Annelie Schiller, Alexander Bertz and Daniel Carl
Appl. Sci. 2018, 8(7), 1042; https://doi.org/10.3390/app8071042 - 26 Jun 2018
Cited by 19 | Viewed by 4069
Abstract
Digital holography is a well-established technology for optical quality control in industrial applications. Two common challenges in digital holographic measurement tasks are the ambiguity at phase steps and the limited depth of focus. With multiwavelength holography, multiple artificial wavelengths are used to extend [...] Read more.
Digital holography is a well-established technology for optical quality control in industrial applications. Two common challenges in digital holographic measurement tasks are the ambiguity at phase steps and the limited depth of focus. With multiwavelength holography, multiple artificial wavelengths are used to extend the sensor’s measurement range up to several millimeters, allowing measurements on rough surfaces. To further extend the unambiguous range, additional highly stabilized and increasingly expensive laser sources can be used. Besides that, unwrapping algorithms can be used to overcome phase ambiguities—but these require continuous objects. With the unique feature of numerical refocusing, digital holography allows the numerical generation of an all-in-focus unambiguous image. We present a shape-from-focus algorithm that allows the extension of the depth of field beyond geometrical imaging limitations and yields unambiguous height information, even across discontinuities. Phase noise is used as a focus criterion and to generate a focus index map. The algorithm’s performance is demonstrated at a gear flank with steep slopes and a step sample with discontinuities far beyond the system’s geometrical limit. The benefit of this method on axially extended objects is discussed. Full article
(This article belongs to the Special Issue Applications of Digital Holographic Microscopy)
Show Figures

Figure 1

19 pages, 3928 KiB  
Article
Multiwavelength Absolute Phase Retrieval from Noisy Diffractive Patterns: Wavelength Multiplexing Algorithm
by Vladimir Katkovnik, Igor Shevkunov, Nikolay V. Petrov and Karen Eguiazarian
Appl. Sci. 2018, 8(5), 719; https://doi.org/10.3390/app8050719 - 04 May 2018
Cited by 14 | Viewed by 3700
Abstract
We study the problem of multiwavelength absolute phase retrieval from noisy diffraction patterns. The system is lensless with multiwavelength coherent input light beams and random phase masks applied for wavefront modulation. The light beams are formed by light sources radiating all wavelengths simultaneously. [...] Read more.
We study the problem of multiwavelength absolute phase retrieval from noisy diffraction patterns. The system is lensless with multiwavelength coherent input light beams and random phase masks applied for wavefront modulation. The light beams are formed by light sources radiating all wavelengths simultaneously. A sensor equipped by a Color Filter Array (CFA) is used for spectral measurement registration. The developed algorithm targeted on optimal phase retrieval from noisy observations is based on maximum likelihood technique. The algorithm is specified for Poissonian and Gaussian noise distributions. One of the key elements of the algorithm is an original sparse modeling of the multiwavelength complex-valued wavefronts based on the complex-domain block-matching 3D filtering. Presented numerical experiments are restricted to noisy Poissonian observations. They demonstrate that the developed algorithm leads to effective solutions explicitly using the sparsity for noise suppression and enabling accurate reconstruction of absolute phase of high-dynamic range. Full article
(This article belongs to the Special Issue Applications of Digital Holographic Microscopy)
Show Figures

Figure 1

10 pages, 8291 KiB  
Article
Calibrated Phase-Shifting Digital Holographic Microscope Using a Sampling Moiré Technique
by Peng Xia, Qinghua Wang, Shien Ri and Hiroshi Tsuda
Appl. Sci. 2018, 8(5), 706; https://doi.org/10.3390/app8050706 - 03 May 2018
Cited by 3 | Viewed by 4175
Abstract
A calibrated phase-shifting digital holographic microscope system capable of improving the quality of reconstructed images is proposed. Phase-shifting errors are introduced in phase-shifted holograms for numerous reasons, such as the non-linearity of piezoelectric transducers (PZTs), wavelength fluctuations in lasers, and environmental disturbances, leading [...] Read more.
A calibrated phase-shifting digital holographic microscope system capable of improving the quality of reconstructed images is proposed. Phase-shifting errors are introduced in phase-shifted holograms for numerous reasons, such as the non-linearity of piezoelectric transducers (PZTs), wavelength fluctuations in lasers, and environmental disturbances, leading to poor-quality reconstructions. In our system, in addition to the camera used to record object information, an extra camera is used to record interferograms, which are used to analyze phase-shifting errors using a sampling Moiré technique. The quality of the reconstructed object images can be improved by the phase-shifting error compensation algorithm. Both the numerical simulation and experiment demonstrate the effectiveness of the proposed system. Full article
(This article belongs to the Special Issue Applications of Digital Holographic Microscopy)
Show Figures

Graphical abstract

Review

Jump to: Research

10 pages, 4173 KiB  
Review
Multi-Modal Ptychography: Recent Developments and Applications
by Xiaowen Shi, Nicolas Burdet, Darren Batey and Ian Robinson
Appl. Sci. 2018, 8(7), 1054; https://doi.org/10.3390/app8071054 - 28 Jun 2018
Cited by 6 | Viewed by 3600
Abstract
Utilisation of partially coherent X-rays in Coherent X-ray Diffraction Imaging (CDI) and X-ray ptychography have brought intense research and developments for the past few years. Generally, data reconstructions convergence can be achieved by considering to incorporate partially coherent X-rays in ptychographic iterative reconstructions [...] Read more.
Utilisation of partially coherent X-rays in Coherent X-ray Diffraction Imaging (CDI) and X-ray ptychography have brought intense research and developments for the past few years. Generally, data reconstructions convergence can be achieved by considering to incorporate partially coherent X-rays in ptychographic iterative reconstructions algorithms. Recently, exploration of both X-ray probe and sample modes have been studied and it was suggested that sufficient constraints are required to achieve accurate data reconstructions due to the mathematical inseparability of multiple sample modes that are superimposed into a single set of ptychographic intensity patterns. Here we review the multi-modal ptychography that can be used as a new emerging technique to investigate dynamical sample systems with partially coherent X-rays by recent experimental and simulated demonstrations. Full article
(This article belongs to the Special Issue Applications of Digital Holographic Microscopy)
Show Figures

Figure 1

16 pages, 1040 KiB  
Review
Quantitative Phase Imaging for Label-Free Analysis of Cancer Cells—Focus on Digital Holographic Microscopy
by Zahra El-Schich, Anna Leida Mölder and Anette Gjörloff Wingren
Appl. Sci. 2018, 8(7), 1027; https://doi.org/10.3390/app8071027 - 23 Jun 2018
Cited by 43 | Viewed by 9289
Abstract
To understand complex biological processes, scientists must gain insight into the function of individual living cells. In contrast to the imaging of fixed cells, where a single snapshot of the cell’s life is retrieved, live-cell imaging allows investigation of the dynamic processes underlying [...] Read more.
To understand complex biological processes, scientists must gain insight into the function of individual living cells. In contrast to the imaging of fixed cells, where a single snapshot of the cell’s life is retrieved, live-cell imaging allows investigation of the dynamic processes underlying the function and morphology of cells. Label-free imaging of living cells is advantageous since it is used without fluorescent probes and maintains an appropriate environment for cellular behavior, otherwise leading to phototoxicity and photo bleaching. Quantitative phase imaging (QPI) is an ideal method for studying live cell dynamics by providing data from noninvasive monitoring over arbitrary time scales. The effect of drugs on migration, proliferation, and apoptosis of cancer cells are emerging fields suitable for QPI analysis. In this review, we provide a current insight into QPI applied to cancer research. Full article
(This article belongs to the Special Issue Applications of Digital Holographic Microscopy)
Show Figures

Figure 1

Back to TopTop