E-Mail Alert

Add your e-mail address to receive forthcoming issues of this journal:

Journal Browser

Journal Browser

Special Issue "Protein-DNA Interactions"

A special issue of Genes (ISSN 2073-4425). This special issue belongs to the section "Molecular Genetics".

Deadline for manuscript submissions: 31 May 2017

Special Issue Editors

Guest Editor
Prof. Dr. Linda Bloom

Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, USA
Website | E-Mail
Interests: Dynamic protein-DNA interactions; DNA replication; DNA repair
Guest Editor
Prof. Dr. Jörg Bungert

Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, USA
Website | E-Mail
Interests: Protein-DNA interactions; synthetic DNA-binding proteins; transcription

Special Issue Information

Dear Colleagues,

The binding of proteins to DNA is critical for maintaining and expressing genetic information. Protein-DNA interactions are involved in condensing chromosomes to fit into cells, regulating the expression of genetic information, duplicating the genome to pass copies to daughter cells, and preserving the structure and integrity of genomic DNA. Our understanding of protein-DNA interactions required for these critical functions has advanced on many levels. Structural approaches have elucidated mechanisms by which proteins physically interact with DNA to perform their functions. Biochemical studies have defined dynamic and transient protein-DNA interactions essential for enzyme transactions on DNA. Recently, exciting progress has been made in two areas: single molecule and in vivo studies of protein-DNA interactions. Single molecule approaches reveal the complex dynamics of molecular interactions between single protein molecules and DNA that are hidden by the ensemble averaging inherent in bulk methods. Observation of the behavior of individual molecules allows us to answer questions that are impossible to address by examining a large population of molecules where the dynamics are unsynchronized, and thus “blur” the picture that we get. Exciting progress has also been made in investigating protein-DNA interactions inside cells in the crowded and complex molecular environment where they naturally function. Researchers have been able to query unique loci to identify protein-DNA interactions that are required for a specific cellular process, and recent advances in DNA sequencing have permitted the development of approaches to map protein-DNA interactions across the genome and in a cell-type specific manner. These studies revealed that DNA shape and DNA motif environment contribute to efficient protein-DNA interactions. Articles in this special issue should provide insight into mechanisms of protein-DNA interactions, and papers using single-molecule approaches or investigating interactions in vivo would be of special interest.

Prof. Dr. Linda Bloom
Prof. Dr. Jörg Bungert
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Genes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

• protein-DNA interactions
• chromatin structure
• transcription
• DNA replication
• DNA repair
• DNA recombination

Published Papers (1 paper)

View options order results:
result details:
Displaying articles 1-1
Export citation of selected articles as:

Review

Open AccessReview Eukaryotic Replicative Helicase Subunit Interaction with DNA and Its Role in DNA Replication
Genes 2017, 8(4), 117; doi:10.3390/genes8040117
Received: 17 February 2017 / Revised: 23 March 2017 / Accepted: 31 March 2017 / Published: 6 April 2017
PDF Full-text (2961 KB) | HTML Full-text | XML Full-text
Abstract
The replicative helicase unwinds parental double-stranded DNA at a replication fork to provide single-stranded DNA templates for the replicative polymerases. In eukaryotes, the replicative helicase is composed of the Cdc45 protein, the heterohexameric ring-shaped Mcm2-7 complex, and the tetrameric GINS complex (CMG). The
[...] Read more.
The replicative helicase unwinds parental double-stranded DNA at a replication fork to provide single-stranded DNA templates for the replicative polymerases. In eukaryotes, the replicative helicase is composed of the Cdc45 protein, the heterohexameric ring-shaped Mcm2-7 complex, and the tetrameric GINS complex (CMG). The CMG proteins bind directly to DNA, as demonstrated by experiments with purified proteins. The mechanism and function of these DNA-protein interactions are presently being investigated, and a number of important discoveries relating to how the helicase proteins interact with DNA have been reported recently. While some of the protein-DNA interactions directly relate to the unwinding function of the enzyme complex, other protein-DNA interactions may be important for minichromosome maintenance (MCM) loading, origin melting or replication stress. This review describes our current understanding of how the eukaryotic replicative helicase subunits interact with DNA structures in vitro, and proposed models for the in vivo functions of replicative helicase-DNA interactions are also described. Full article
(This article belongs to the Special Issue Protein-DNA Interactions)
Figures

Figure 1

Journal Contact

MDPI AG
Genes Editorial Office
St. Alban-Anlage 66, 4052 Basel, Switzerland
E-Mail: 
Tel. +41 61 683 77 34
Fax: +41 61 302 89 18
Editorial Board
Contact Details Submit to Special Issue Edit a special issue Review for Genes
loading...
Back to Top