Research

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Open AccessArticle Economic Optimized Medium for Tensio-Active Agent Production by Candida sphaerica UCP0995 and Application in the Removal of Hydrophobic Contaminant from Sand

by Juliana M. Luna, Raquel D. Rufino, Clarissa D.C. Albuquerque, Leonie A. Sarubbo and Galba M. Campos-Takaki

Int. J. Mol. Sci. 2011, 12(4), 2463-2476; https://doi.org/10.3390/ijms12042463

Received: 1 March 2011 / Revised: 14 March 2011 / Accepted: 23 March 2011 / Published: 8 April 2011

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Abstract

Statistical experimental designs and response surface methodology were employed to optimize the concentrations of agroindustrial residues as soybean oil (SORR) from refinery, and corn steep liquor (CSL) from corn industry, for tensio-active agent produced by Candida sphaerica UCP 0995. Three 2²full

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Statistical experimental designs and response surface methodology were employed to optimize the concentrations of agroindustrial residues as soybean oil (SORR) from refinery, and corn steep liquor (CSL) from corn industry, for tensio-active agent produced by Candida sphaerica UCP 0995. Three 2²full factorial design were applied sequentially to investigate the effects of the concentrations and interactions of soybean oil refinery residue and corn steep liquor on the surface tension of free-cell culture broth for 144 h. Two 2² central composite designs and response surface methodology were adopted to derive a statistical model to measure the effect of SORR and CSL on the surface tension of the free-cell culture broth for 144 h. The regression equation obtained from the experimental data using a central composite design was solved, and by analyzing the response surface contour plots, the optimal concentrations of the constituents of the medium were determined: 8.63% v/v (≈9% v/v) of SORR and 8.80% v/v (≈9% v/v) CSL. The minimum surface tension predicted and experimentally confirmed was 25.25 mN/m. The new biosurfactant, denominated Lunasan, recovered 95% of motor oil adsorbed in a sand sample, thus showing great potential for use in bioremediation processes, especially in the petroleum industry. Full article

(This article belongs to the Special Issue Biosurfactants)

Open AccessArticle Novel Application of Cyclolipopeptide Amphisin: Feasibility Study as Additive to Remediate Polycyclic Aromatic Hydrocarbon (PAH) Contaminated Sediments

by Anne Groboillot, Florence Portet-Koltalo, Franck Le Derf, Marc J.G. Feuilloley, Nicole Orange and Cécile Duclairoir Poc

Int. J. Mol. Sci. 2011, 12(3), 1787-1806; https://doi.org/10.3390/ijms12031787

Received: 3 November 2010 / Revised: 15 February 2011 / Accepted: 2 March 2011 / Published: 9 March 2011

Cited by 7 | PDF Full-text (703 KB) | HTML Full-text | XML Full-text

Abstract

To decontaminate dredged harbor sediments by bioremediation or electromigration processes, adding biosurfactants could enhance the bioavailability or mobility of contaminants in an aqueous phase. Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments

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To decontaminate dredged harbor sediments by bioremediation or electromigration processes, adding biosurfactants could enhance the bioavailability or mobility of contaminants in an aqueous phase. Pure amphisin from Pseudomonas fluorescens DSS73 displays increased effectiveness in releasing polycyclic aromatic hydrocarbons (PAHs) strongly adsorbed to sediments when compared to a synthetic anionic surfactant. Amphisin production by the bacteria in the natural environment was also considered. DSS73’s growth is weakened by three model PAHs above saturation, but amphisin is still produced. Estuarine water feeding the dredged material disposal site of a Norman harbor (France) allows both P. fluorescens DSS73 growth and amphisin production. Full article

(This article belongs to the Special Issue Biosurfactants)

Open AccessArticle Importance of the Long-Chain Fatty Acid Beta-Hydroxylating Cytochrome P450 Enzyme YbdT for Lipopeptide Biosynthesis in Bacillus subtilis Strain OKB105

by Noha H. Youssef, Neil Wofford and Michael J. McInerney

Int. J. Mol. Sci. 2011, 12(3), 1767-1786; https://doi.org/10.3390/ijms12031767

Received: 9 December 2010 / Revised: 8 February 2011 / Accepted: 3 March 2011 / Published: 8 March 2011

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Abstract

Bacillus species produce extracellular, surface-active lipopeptides such as surfactin that have wide applications in industry and medicine. The steps involved in the synthesis of 3-hydroxyacyl-coenzyme A (CoA) substrates needed for surfactin biosynthesis are not understood. Cell-free extracts of Bacillus subtilis strain OKB105 synthesized

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Bacillus species produce extracellular, surface-active lipopeptides such as surfactin that have wide applications in industry and medicine. The steps involved in the synthesis of 3-hydroxyacyl-coenzyme A (CoA) substrates needed for surfactin biosynthesis are not understood. Cell-free extracts of Bacillus subtilis strain OKB105 synthesized lipopeptide biosurfactants in presence of L-amino acids, myristic acid, coenzyme A, ATP, and H₂O₂, which suggested that 3-hydroxylation occurs prior to CoA ligation of the long chain fatty acids (LCFAs). We hypothesized that YbdT, a cytochrome P450 enzyme known to beta-hydroxylate LCFAs, functions to form 3-hydroxy fatty acids for lipopeptide biosynthesis. An in-frame mutation of ybdT was constructed and the resulting mutant strain (NHY1) produced predominantly non-hydroxylated lipopeptide with diminished biosurfactant and beta-hemolytic activities. Mass spectrometry showed that 95.6% of the fatty acids in the NHY1 biosurfactant were non-hydroxylated compared to only ~61% in the OKB105 biosurfactant. Cell-free extracts of the NHY1 synthesized surfactin containing 3-hydroxymyristic acid from 3-hydroxymyristoyl-CoA at a specific activity similar to that of the wild type (17 ± 2 versus 17.4 ± 6 ng biosurfactant min⁻¹·ng·protein⁻¹, respectively). These results showed that the mutation did not affect any function needed to synthesize surfactin once the 3-hydroxyacyl-CoA substrate was formed and that YbdT functions to supply 3-hydroxy fatty acid for surfactin biosynthesis. The fact that YbdT is a peroxidase could explain why biosurfactant production is rarely observed in anaerobically grown Bacillus species. Manipulation of LCFA specificity of YbdT could provide a new route to produce biosurfactants with activities tailored to specific functions. Full article

(This article belongs to the Special Issue Biosurfactants)

Open AccessArticle Rhamnolipid Biosurfactants as New Players in Animal and Plant Defense against Microbes

by Parul Vatsa, Lisa Sanchez, Christophe Clement, Fabienne Baillieul and Stephan Dorey

Int. J. Mol. Sci. 2010, 11(12), 5095-5108; https://doi.org/10.3390/ijms11125095

Received: 1 November 2010 / Revised: 15 November 2010 / Accepted: 1 December 2010 / Published: 9 December 2010

Cited by 61 | PDF Full-text (211 KB) | HTML Full-text | XML Full-text

Abstract

Rhamnolipids are known as very efficient biosurfactant molecules. They are used in a wide range of industrial applications including food, cosmetics, pharmaceutical formulations and bioremediation of pollutants. The present review provides an overview of the effect of rhamnolipids in animal and plant defense

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Rhamnolipids are known as very efficient biosurfactant molecules. They are used in a wide range of industrial applications including food, cosmetics, pharmaceutical formulations and bioremediation of pollutants. The present review provides an overview of the effect of rhamnolipids in animal and plant defense responses. We describe the current knowledge on the stimulation of plant and animal immunity by these molecules, as well as on their direct antimicrobial properties. Given their ecological acceptance owing to their low toxicity and biodegradability, rhamnolipids have the potential to be useful molecules in medicine and to be part of alternative strategies in order to reduce or replace pesticides in agriculture. Full article

(This article belongs to the Special Issue Biosurfactants)

Open AccessArticle Production and Characterization of Fengycin by Indigenous Bacillus subtilis F29-3 Originating from a Potato Farm

by Yu-Hong Wei, Li-Chuan Wang, Wei-Chuan Chen and Shan-Yu Chen

Int. J. Mol. Sci. 2010, 11(11), 4526-4538; https://doi.org/10.3390/ijms11114526

Received: 8 October 2010 / Revised: 24 October 2010 / Accepted: 10 November 2010 / Published: 12 November 2010

Cited by 21 | PDF Full-text (1022 KB) | HTML Full-text | XML Full-text | Supplementary Files

Abstract

Fengycin, a lipopeptide biosurfactant, was produced by indigenous Bacillus subtilis F29-3 isolated from a potato farm. Although inhibiting the growth of filamentous fungi, the fengycin is ineffective against yeast and bacteria. In this study, fengycin was isolated from fermentation broth of B. subtilis

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Fengycin, a lipopeptide biosurfactant, was produced by indigenous Bacillus subtilis F29-3 isolated from a potato farm. Although inhibiting the growth of filamentous fungi, the fengycin is ineffective against yeast and bacteria. In this study, fengycin was isolated from fermentation broth of B. subtilis F29-3 via acidic precipitation (pH 2.0 with 5 N HCl) followed by purification using ultrafiltration and nanofiltration. The purified fengycin product was characterized qualitatively by using fast atom bombardment-mass spectrometer, Fourier transform infrared spectrometer, ultraviolet-visible spectrophotometer, ¹³C-nuclear magnetic resonance spectrometer and matrix assisted laser desorption ionization-time of flight, followed by quantitative analysis using reversed-phase HPLC system. This study also attempted to increase fengycin production by B. subtilis F29-3 in order to optimize the fermentation medium constituents. The fermentation medium composition was optimized using response surface methodology (RSM) to increase fengycin production from B. subtilis F29-3. According to results of the five-level four-factor central composite design, the composition of soybean meal, NaNO₃, MnSO₄·4H₂O, mannitol-mannitol, soybean meal-mannitol, soybean meal‑soybean meal, NaNO₃-NaNO₃ and MnSO₄·4H₂O-MnSO₄·4H₂O significantly affected production. The simulation model produced a coefficient of determination (R²) of 0.9043, capable of accounting for 90.43% variability of the data. Results of the steepest ascent and central composite design indicated that 26.2 g/L of mannitol, 21.9 g/L of soybean meal, 3.1 g/L of NaNO₃ and 0.2 g/L of MnSO₄·4H₂O represented the optimal medium composition, leading to the highest production of fengycin. Furthermore, the optimization strategy increased the fengycin production from 1.2 g/L to 3.5 g/L. Full article

(This article belongs to the Special Issue Biosurfactants)

Open AccessArticle Foam Properties and Detergent Abilities of the Saponins from Camellia oleifera

by Yu-Fen Chen, Chao-Hsun Yang, Ming-Shiang Chang, Yong-Ping Ciou and Yu-Chun Huang

Int. J. Mol. Sci. 2010, 11(11), 4417-4425; https://doi.org/10.3390/ijms11114417

Received: 14 October 2010 / Revised: 30 October 2010 / Accepted: 1 November 2010 / Published: 4 November 2010

Cited by 63 | PDF Full-text (190 KB) | HTML Full-text | XML Full-text

Abstract

The defatted seed meal of Camellia oleifera has been used as a natural detergent and its extract is commercially utilized as a foam-stabilizing and emulsifying agent. The goal of this study was to investigate the foam properties and detergent ability of the saponins

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The defatted seed meal of Camellia oleifera has been used as a natural detergent and its extract is commercially utilized as a foam-stabilizing and emulsifying agent. The goal of this study was to investigate the foam properties and detergent ability of the saponins from the defatted seed meal of C. oleifera. The crude saponin content in the defatted seed meal of C. oleifera was 8.34% and the total saponins content in the crude saponins extract was 39.5% (w/w). The foaming power of the 0.5% crude saponins extract solution from defatted seed meal of C. oleifera was 37.1% of 0.5% SLS solution and 51.3% to that of 0.5% Tween 80 solution. The R5 value of 86.0% represents good foam stability of the crude saponins extracted from the defatted seed meal of the plant. With the reduction of water surface tension from 72 mN/m to 50.0 mN/m, the 0.5% crude saponins extract solution has wetting ability. The sebum-removal experiment indicated that the crude saponins extract has moderate detergency. The detergent abilities of the saponins from C. oleifera and Sapindus mukorossi were also compared. Full article

(This article belongs to the Special Issue Biosurfactants)

Review

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Open AccessReview Characterization and Emulsification Properties of Rhamnolipid and Sophorolipid Biosurfactants and Their Applications

by Thu T. Nguyen and David A. Sabatini

Int. J. Mol. Sci. 2011, 12(2), 1232-1244; https://doi.org/10.3390/ijms12021232

Received: 2 November 2010 / Revised: 9 February 2011 / Accepted: 12 February 2011 / Published: 18 February 2011

Cited by 24 | PDF Full-text (510 KB) | HTML Full-text | XML Full-text

Abstract

Due to their non-toxic nature, biodegradability and production from renewable resources, research has shown an increasing interest in the use of biosurfactants in a wide variety of applications. This paper reviews the characterization of rhamnolipid and sophorolipid biosurfactants based on their hydrophilicity/hydrophobicity and

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Due to their non-toxic nature, biodegradability and production from renewable resources, research has shown an increasing interest in the use of biosurfactants in a wide variety of applications. This paper reviews the characterization of rhamnolipid and sophorolipid biosurfactants based on their hydrophilicity/hydrophobicity and their ability to form microemulsions with a range of oils without additives. The use of the biosurfactants in applications such as detergency and vegetable oil extraction for biodiesel application is also discussed. Rhamnolipid was found to be a hydrophilic surfactant while sophorolipid was found to be very hydrophobic. Therefore, rhamnolipid and sophorolipid biosurfactants in mixtures showed robust performance in these applications. Full article

(This article belongs to the Special Issue Biosurfactants)

Open AccessReview Environmental Applications of Biosurfactants: Recent Advances

by Magdalena Pacwa-Płociniczak, Grażyna A. Płaza, Zofia Piotrowska-Seget and Swaranjit Singh Cameotra

Int. J. Mol. Sci. 2011, 12(1), 633-654; https://doi.org/10.3390/ijms12010633

Received: 30 November 2010 / Revised: 8 December 2010 / Accepted: 10 January 2011 / Published: 18 January 2011

Cited by 271 | PDF Full-text (589 KB) | HTML Full-text | XML Full-text

Abstract

Increasing public awareness of environmental pollution influences the search and development of technologies that help in clean up of organic and inorganic contaminants such as hydrocarbons and metals. An alternative and eco-friendly method of remediation technology of environments contaminated with these pollutants is

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Increasing public awareness of environmental pollution influences the search and development of technologies that help in clean up of organic and inorganic contaminants such as hydrocarbons and metals. An alternative and eco-friendly method of remediation technology of environments contaminated with these pollutants is the use of biosurfactants and biosurfactant-producing microorganisms. The diversity of biosurfactants makes them an attractive group of compounds for potential use in a wide variety of industrial and biotechnological applications. The purpose of this review is to provide a comprehensive overview of advances in the applications of biosurfactants and biosurfactant-producing microorganisms in hydrocarbon and metal remediation technologies. Full article

(This article belongs to the Special Issue Biosurfactants)

Open AccessReview Biosurfactants for Microbubble Preparation and Application

by Qingyi Xu, Mitsutoshi Nakajima, Zengshe Liu and Takeo Shiina

Int. J. Mol. Sci. 2011, 12(1), 462-475; https://doi.org/10.3390/ijms12010462

Received: 17 December 2010 / Revised: 11 January 2011 / Accepted: 11 January 2011 / Published: 17 January 2011

Cited by 31 | PDF Full-text (398 KB) | HTML Full-text | XML Full-text

Abstract

Biosurfactants can be classified by their chemical composition and their origin. This review briefly describes various classes of biosurfactants based on their origin and introduces a few of the most widely used biosurfactants. The current status and future trends in biosurfactant production are

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Biosurfactants can be classified by their chemical composition and their origin. This review briefly describes various classes of biosurfactants based on their origin and introduces a few of the most widely used biosurfactants. The current status and future trends in biosurfactant production are discussed, with an emphasis on those derived from plants. Following a brief introduction of the properties of microbubbles, recent progress in the application of microbubble technology to molecular imaging, wastewater treatment, and aerobic fermentation are presented. Several studies on the preparation, characterization and applications of biosurfactant-based microbubbles are reviewed. Full article

(This article belongs to the Special Issue Biosurfactants)

Open AccessReview Diversity of Nonribosomal Peptide Synthetases Involved in the Biosynthesis of Lipopeptide Biosurfactants

by Niran Roongsawang, Kenji Washio and Masaaki Morikawa

Int. J. Mol. Sci. 2011, 12(1), 141-172; https://doi.org/10.3390/ijms12010141

Received: 19 October 2010 / Revised: 3 December 2010 / Accepted: 10 December 2010 / Published: 30 December 2010

Cited by 84 | PDF Full-text (567 KB) | HTML Full-text | XML Full-text

Abstract

Lipopeptide biosurfactants (LPBSs) consist of a hydrophobic fatty acid portion linked to a hydrophilic peptide chain in the molecule. With their complex and diverse structures, LPBSs exhibit various biological activities including surface activity as well as anti-cellular and anti-enzymatic activities. LPBSs are also

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Lipopeptide biosurfactants (LPBSs) consist of a hydrophobic fatty acid portion linked to a hydrophilic peptide chain in the molecule. With their complex and diverse structures, LPBSs exhibit various biological activities including surface activity as well as anti-cellular and anti-enzymatic activities. LPBSs are also involved in multi-cellular behaviors such as swarming motility and biofilm formation. Among the bacterial genera, Bacillus (Gram-positive) and Pseudomonas (Gram-negative) have received the most attention because they produce a wide range of effective LPBSs that are potentially useful for agricultural, chemical, food, and pharmaceutical industries. The biosynthetic mechanisms and gene regulation systems of LPBSs have been extensively analyzed over the last decade. LPBSs are generally synthesized in a ribosome-independent manner with megaenzymes called nonribosomal peptide synthetases (NRPSs). Production of active‑form NRPSs requires not only transcriptional induction and translation but also post‑translational modification and assemblage. The accumulated knowledge reveals the versatility and evolutionary lineage of the NRPSs system. This review provides an overview of the structural and functional diversity of LPBSs and their different biosynthetic mechanisms in Bacillus and Pseudomonas, including both typical and unique systems. Finally, successful genetic engineering of NRPSs for creating novel lipopeptides is also discussed. Full article

(This article belongs to the Special Issue Biosurfactants)

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Review

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Guest Editor Prof. Dr. Yoshie Maitani Institute of Medicinal Chemistry, Fine Drug Targeting Research Laboratory, Hoshi University, Ebara 2-4-41, Shinagawa, Tokyo 142-8501, Japan E-Mail
Guest Editor Dr. Laurence G. Rahme Harvard Medical School, 340 Thier Research Building, 50 Blossom Street, Massachusetts General Hospital, Boston, MA 02114, USA Website \| E-Mail Fax: +1 617 724 8558 Interests: bacterial pathogenesis; multi-host pathogenesis; host-pathogen interactions; Pseudomonas aeruginosa; Acinetobacter; Quorum sensing; small excreted molecules; virulence factors; anti-infectives, anti-virulence drugs; Antibiotic tolerance; models of infections; Drosophila; intestinal infections; burn injury; wound infections; muscle response to infection; bacterial transcriptional regulation
Co-Guest Editor Prof. Dr. Jan Michiels Centre of Microbial and Plant Genetics, Department of Microbial and Molecular Systems, K.U.Leuven, Kasteelpark Arenberg 20 box 2460, B-3001 Heverlee, Belgium Website \| E-Mail