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Advances in Cellular and Molecular Life Sciences

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: closed (31 March 2011) | Viewed by 9267

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Department of Pharmacology and Therapeutics, The University of Melbourne, Parkville, VIC 3010, Australia
Interests: purinergic signalling; central nervous system
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Article
Cloning and Polymorphisms of Yak Lactate Dehydrogenase b Gene
by Guosheng Wang, Xingbo Zhao, Juming Zhong, Meng Cao, Qinghua He, Zhengxin Liu, Yaqiu Lin, Yaou Xu and Yucai Zheng
Int. J. Mol. Sci. 2013, 14(6), 11994-12003; https://doi.org/10.3390/ijms140611994 - 05 Jun 2013
Cited by 4 | Viewed by 8935
Abstract
The main objective of this work was to study the unique polymorphisms of the lactate dehydrogenase-1 (LDH1) gene in yak (Bos grunniens). Native polyacrylamide gel electrophoresis revealed three phenotypes of LDH1 (a tetramer of H subunit) in yak heart and longissimus [...] Read more.
The main objective of this work was to study the unique polymorphisms of the lactate dehydrogenase-1 (LDH1) gene in yak (Bos grunniens). Native polyacrylamide gel electrophoresis revealed three phenotypes of LDH1 (a tetramer of H subunit) in yak heart and longissimus muscle extracts. The corresponding gene, ldhb, encoding H subunits of three LDH1 phenotypes was obtained by RT-PCR. A total of six nucleotide differences were detected in yak ldhb compared with that of cattle, of which five mutations cause amino acid substitutions. Sequence analysis shows that the G896A and C689A, mutations of ldhb gene, result in alterations of differently charged amino acids, and create the three phenotypes (F, M, and S) of yak LDH1. Molecular modeling of the H subunit of LDH indicates that the substituted amino acids are not located within NAD+ or substrate binding sites. PCR-RFLP examination of G896A mutation demonstrated that most LDH1-F samples are actually heterozygote at this site. These results help to elucidate the molecular basis and genetic characteristic of the three unique LDH1 phenotypes in yak. Full article
(This article belongs to the Special Issue Advances in Cellular and Molecular Life Sciences)
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