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Bioactive and Therapeutic Dental Materials

A special issue of Materials (ISSN 1996-1944). This special issue belongs to the section "Biomaterials".

Deadline for manuscript submissions: closed (5 April 2019) | Viewed by 73659

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Special Issue Editor

Dental Biomaterials and Minimally Invasive Dentistry, Departmento de Odontologia, Facultad de Ciencias de la Salud Universidad, CEU-Cardenal Herrera, Alfara del Patriarca, 46115 Valencia, Spain
Interests: enamel-dentin adhesion; dentine permeability, remineralisation; minimally invasive cariology and restorative dentistry
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

A new generation of dental materials has been developed during the last ten years. These are identified as bioactive dental materials, which are able to release calcium, phosphate and other specific ions to help rebuild demineralized dentin and enamel. Such a phenomenon is known as biomineralization, which refers to the exchange of therapeutic ions with the dental substrates forming new apatite or, in many cases, repairing existing demineralized apatite. Moreover, smart materials can react to pH changes in the oral environment, as well as elicit reparative processes within the bonding interface in the presence of body fluids such as saliva, crevicular fluid and blood.

This Special Issue will focus on bioactive dental materials for application in preventive and restorative dentistry, as well as in endodontics in the form of adhesives, resin-based composites, pastes, varnishes, liners and dental cements. Special attention will be given to bioactive materials developed to induce cells differentiation/stimulation, hard tissue formation and exert antimicrobial actions. New innovations are necessary to continue to help reinforng existing technologies and to introduce new paradigms for treating dental disease and restoring teeth seriously comporomised by caries lesions via biomimetic and more biological operative approches.

Prof. Salvatore Sauro
Guest Editor

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Keywords

  • Remineralization
  • Antibacterial
  • Tissue engineering
  • Regeneration
  • Bioactive
  • Biocompatibility Therapeutic dental materials

Published Papers (15 papers)

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14 pages, 2228 KiB  
Article
Effects of Ions-Releasing Restorative Materials on the Dentine Bonding Longevity of Modern Universal Adhesives after Load-Cycle and Prolonged Artificial Saliva Aging
by Salvatore Sauro, Irina Makeeva, Vicente Faus-Matoses, Federico Foschi, Massimo Giovarruscio, Paula Maciel Pires, Maria Elisa Martins Moura, Aline Almeida Neves and Vicente Faus-Llácer
Materials 2019, 12(5), 722; https://doi.org/10.3390/ma12050722 - 01 Mar 2019
Cited by 25 | Viewed by 5094
Abstract
This study aimed at evaluating the microtensile bond strength (MTBS) and fractographic features of dentine-bonded specimens created using universal adhesives applied in etch-and-rinse (ER) or self-etching (SE) mode in combination with modern ion-releasing resin-modified glass-ionomer cement (RMGIC)-based materials after load cycling and artificial [...] Read more.
This study aimed at evaluating the microtensile bond strength (MTBS) and fractographic features of dentine-bonded specimens created using universal adhesives applied in etch-and-rinse (ER) or self-etching (SE) mode in combination with modern ion-releasing resin-modified glass-ionomer cement (RMGIC)-based materials after load cycling and artificial saliva aging. Two universal adhesives (FTB: Futurabond M+, VOCO, Germany; SCU: Scotchbond Universal, 3M Oral Care, USA) were used. Composite build-ups were made with conventional nano-filled composite (AURA, SDI, Australia), conventional resin-modified glass ionomer cement (Ionolux VOCO, Germany), or a (RMGIC)-based composite (ACTIVA, Pulpdent, USA). The specimens were divided in three groups and immersed in deionized water for 24 h, load-cycled (350,000 cycles; 3 Hz; 70 N), or load-cycled and cut into matchsticks and finally immersed for 8 months in artificial saliva (AS). The specimens were cut into matchsticks and tested for microtensile bond strength. The results were analyzed statistically using three-way ANOVA and Fisher’s LSD post hoc test (p < 0.05). Fractographic analysis was performed through stereomicroscope and FE-SEM. FTB showed no significant drop in bond strength after aging. Unlike the conventional composite, the two RMGIC-based materials caused no bond strength reduction in SCU after load-cycle aging and after prolonged aging (8 months). The SEM fractographic analysis showed severe degradation, especially with composite applied on dentine bonded with SCU in ER mode; such degradation was less evident with the two GIC-based materials. The dentine-bond longevity may be influenced by the composition rather than the mode of application (ER vs. SE) of the universal adhesives. Moreover, the choice of the restorative material may play an important role on the longevity of the finalrestoration. Indeed, bioactive GIC-based materials may contribute to maintain the bonding performance of simplified universal adhesives over time, especially when these bonding systems are applied in ER mode. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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13 pages, 2431 KiB  
Article
Bone Healing in Rabbit Calvaria Defects Using a Synthetic Bone Substitute: A Histological and Micro-CT Comparative Study
by Minas Leventis, Peter Fairbairn, Chas Mangham, Antonios Galanos, Orestis Vasiliadis, Danai Papavasileiou and Robert Horowitz
Materials 2018, 11(10), 2004; https://doi.org/10.3390/ma11102004 - 17 Oct 2018
Cited by 25 | Viewed by 8132
Abstract
Bioactive alloplastic materials, like beta-tricalcium phosphate (β-TCP) and calcium sulfate (CS), have been extensively researched and are currently used in orthopedic and dental bone regenerative procedures. The purpose of this study was to compare the performance of EthOss versus a bovine xenograft and [...] Read more.
Bioactive alloplastic materials, like beta-tricalcium phosphate (β-TCP) and calcium sulfate (CS), have been extensively researched and are currently used in orthopedic and dental bone regenerative procedures. The purpose of this study was to compare the performance of EthOss versus a bovine xenograft and spontaneous healing. The grafting materials were implanted in standardized 8 mm circular bicortical bone defects in rabbit calvariae. A third similar defect in each animal was left empty for natural healing. Six male rabbits were used. After eight weeks of healing, the animals were euthanized and the bone tissue was analyzed using histology and micro-computed tomography (micro-CT). Defects treated with β-TCP/CS showed the greatest bone regeneration and graft resorption, although differences between groups were not statistically significant. At sites that healed spontaneously, the trabecular number was lower (p < 0.05) and trabecular separation was higher (p < 0.05), compared to sites treated with β-TCP/CS or xenograft. Trabecular thickness was higher at sites treated with the bovine xenograft (p < 0.05) compared to sites filled with β-TCP/CS or sites that healed spontaneously. In conclusion, the novel β-TCP/CS grafting material performed well as a bioactive and biomimetic alloplastic bone substitute when used in cranial defects in this animal model. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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14 pages, 2496 KiB  
Article
Effects of Polyacrylic Acid Pre-Treatment on Bonded-Dentine Interfaces Created with a Modern Bioactive Resin-Modified Glass Ionomer Cement and Subjected to Cycling Mechanical Stress
by Salvatore Sauro, Vicente Faus-Matoses, Irina Makeeva, Juan Manuel Nuñez Martí, Raquel Gonzalez Martínez, José Antonio García Bautista and Vicente Faus-Llácer
Materials 2018, 11(10), 1884; https://doi.org/10.3390/ma11101884 - 02 Oct 2018
Cited by 10 | Viewed by 3614
Abstract
Objectives: Resin-modified glass ionomer cements (RMGIC) are considered excellent restorative materials with unique therapeutic and anti-cariogenic activity. However, concerns exist regarding the use of polyacrylic acid as a dentine conditioner as it may influence the bonding performance of RMGIC. The aim of this [...] Read more.
Objectives: Resin-modified glass ionomer cements (RMGIC) are considered excellent restorative materials with unique therapeutic and anti-cariogenic activity. However, concerns exist regarding the use of polyacrylic acid as a dentine conditioner as it may influence the bonding performance of RMGIC. The aim of this study was to evaluate the effect of different protocols for cycling mechanical stress on the bond durability and interfacial ultramorphology of a modern RMGIC applied to dentine pre-treated with/without polyacrylic acid conditioner (PAA). Methods: The RMGIC was applied onto human dentine specimens prepared with silicon-carbide (SiC) abrasive paper with or without the use of a PAA conditioner. The specimens were immersed in deionised water for 24 h then divided in 3 groups. The first group was cut into matchsticks (cross-sectional area of 0.9 mm2) and tested immediately for microtensile bond strength (MTBS). The second was first subjected to load cycling (250,000 cycles; 3 Hz; 70 N) and then cut into matchsticks and tested for MTBS. The third group was subjected to load cycling (250,000 cycles; 3 Hz; 70 N), cut into matchsticks, and then immersed for 8 months storage in artificial saliva (AS); these were finally tested for MTBS. The results were analysed statistically using two-way ANOVA and the Student–Newman–Keuls test (α = 0.05). Fractographic analysis was performed using FE-SEM, while further RMCGIC-bonded dentine specimens were aged as previously described and used for interfacial ultramorphology characterisation (dye nanoleakage) using confocal microscopy. Results: The RMGIC applied onto dentine that received no pre-treatment (10% PAA gel) showed no significant reduction in MTBS after load cycling followed by 8 months of storage in AS (p > 0.05). The RMGIC–dentine interface created in PAA-conditioned SiC-abraded dentine specimens showed no sign of degradation, but with porosities within the bonding interface both after load cycling and after 8 months of storage in AS. Conversely, the RMGIC–dentine interface of the specimens with no PAA pre-treatment showed no sign of porosity within the interface after any of the aging protocols, although some bonded-dentine interfaces presented cohesive cracks within the cement after prolonged AS storage. However, the specimens of this group showed no significant reduction in bond strength (p < 0.05) after 8 months of storage in AS or load cycling (p > 0.05). After prolonged AS storage, the bond strength value attained in RMGIC–dentine specimens created in PAA pre-treated dentine were significantly higher than those observed in the specimens created with no PAA pre-treatment in dentine. Conclusions: PAA conditioning of dentine prior to application of RMGIC induces no substantial effect on the bond strength after short-term storage, but its use may increase the risk of collagen degradation at the bonding interface after prolonged aging. Modern RMGIC applied without PAA dentine pre-treatment may have greater therapeutic synergy with saliva during cycle occlusal load, thereby enhancing the remineralisation and protection of the bonding interface. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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14 pages, 1822 KiB  
Article
Biomimetic Mineralizing Agents Recover the Micro Tensile Bond Strength of Demineralized Dentin
by Luiz Filipe Barbosa-Martins, Jossaria Pereira de Sousa, Lívia Araújo Alves, Robert Philip Wynn Davies and Regina Maria Puppin-Rontanti
Materials 2018, 11(9), 1733; https://doi.org/10.3390/ma11091733 - 14 Sep 2018
Cited by 17 | Viewed by 5148
Abstract
Biomimetic remineralization is an approach that mimics natural biomineralization, and improves adhesive procedures. The aim of this paper was to investigate the influence of Dentin Caries-like Lesions (DCLL)-Producing Model on microtensile bond strength (μTBS) of etch and rinse adhesive systems and investigate the [...] Read more.
Biomimetic remineralization is an approach that mimics natural biomineralization, and improves adhesive procedures. The aim of this paper was to investigate the influence of Dentin Caries-like Lesions (DCLL)-Producing Model on microtensile bond strength (μTBS) of etch and rinse adhesive systems and investigate the effect of remineralizing agents such as Sodium Fluoride (NaF), MI Paste™ (MP) and Curodont™ Repair (CR) on caries-affected dentin (n = 6). Nine groups were established: (1) Sound dentin; (2) Demineralized dentin/Chemical DCLL: (3) Demineralized dentin/Biological DCLL; (4) Chemical/DCLL + NaF; (5) Chemical/DCLL + MP; (6) Chemical/DCLL + CR; (7) Biological/DCLL + NaF; (8) Biological/DCLL + MP; (9) Biological/DCLL + CR. Then all dentin blocks were subjected to a bonding procedure with Adper™ Single Bond 2 adhesive system/Filtek Z350XT 4 mm high block, following this they were immersed in deionized water/24 h and then sectioned with ≅1 mm2 beams. The μTBS test was conducted at 1 mm/min/500 N loading. Failure sites were evaluated by SEM (scanning electron microscopy (150×). μTBS data were submitted to factorial ANOVA and Tukey’s test (p < 0.05). The highest values were found when demineralized dentin was treated with MP and CR, regardless caries lesion depth (p < 0.05). There was a predominance of adhesive/mixed in the present study. It was concluded that the use of the artificial dentin caries production models produces differences in the μTBS. Additionally MP and CR remineralizing agents could enhance adhesive procedures even at different models of caries lesion. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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13 pages, 1756 KiB  
Article
Tuning Nano-Amorphous Calcium Phosphate Content in Novel Rechargeable Antibacterial Dental Sealant
by Maria Salem Ibrahim, Faisal D. AlQarni, Yousif A. Al-Dulaijan, Michael D. Weir, Thomas W. Oates, Hockin H. K. Xu and Mary Anne S. Melo
Materials 2018, 11(9), 1544; https://doi.org/10.3390/ma11091544 - 27 Aug 2018
Cited by 41 | Viewed by 5406
Abstract
Dental sealants with antibacterial and remineralizing properties are promising for caries prevention among children and adolescents. The application of nanotechnology and polymer development have enabled nanoparticles of amorphous calcium phosphate (NACP) and dimethylaminohexadecyl methacrylate (DMAHDM) to emerge as anti-caries strategies via resin-based dental [...] Read more.
Dental sealants with antibacterial and remineralizing properties are promising for caries prevention among children and adolescents. The application of nanotechnology and polymer development have enabled nanoparticles of amorphous calcium phosphate (NACP) and dimethylaminohexadecyl methacrylate (DMAHDM) to emerge as anti-caries strategies via resin-based dental materials. Our objectives in this study were to (1) incorporate different mass fractions of NACP into a parental rechargeable and antibacterial sealant; (2) investigate the effects on mechanical performance, and (3) assess how the variations in NACP concentration would affect the calcium (Ca) and phosphate (PO4) ion release and re-chargeability over time. NACP were synthesized using a spray-drying technique and incorporated at mass fractions of 0, 10, 20 and 30%. Flexural strength, flexural modulus, and flowability were assessed for mechanical and physical performance. Ca and PO4 ion release were measured over 70 days, and three ion recharging cycles were performed for re-chargeability. The impact of the loading percentage of NACP upon the sealant’s performance was evaluated, and the optimized formulation was eventually selected. The experimental sealant at 20% NACP had flexural strength and flexural modulus of 79.5 ± 8.4 MPa and 4.2 ± 0.4 GPa, respectively, while the flexural strength and flexural modulus of a commercial sealant control were 70.7 ± 5.5 MPa (p > 0.05) and 3.3 ± 0.5 GPa (p < 0.05), respectively. A significant reduction in flow was observed in the experimental sealant at 30% NACP (p < 0.05). Increasing the NACP mass fraction increased the ion release. The sealant formulation with NACP at 20% displayed desirable mechanical performance and ideal flow and handling properties, and also showed high levels of long-term Ca and PO4 ion release and excellent recharge capabilities. The findings provide fundamental data for the development of a new generation of antibacterial and rechargeable Ca and PO4 dental sealants to promote remineralization and inhibit caries. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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14 pages, 6415 KiB  
Article
Preclinical Studies of the Biosafety and Efficacy of Human Bone Marrow Mesenchymal Stem Cells Pre-Seeded into β-TCP Scaffolds after Transplantation
by Mar Gonzálvez-García, Carlos M. Martinez, Victor Villanueva, Ana García-Hernández, Miguel Blanquer, Luis Meseguer-Olmo, Ricardo E. Oñate Sánchez, José M. Moraleda and Francisco Javier Rodríguez-Lozano
Materials 2018, 11(8), 1349; https://doi.org/10.3390/ma11081349 - 03 Aug 2018
Cited by 6 | Viewed by 3534
Abstract
Background: Cell-Based Therapies (CBT) constitute a valid procedure for increasing the quantity and quality of bone in areas with an inadequate bone volume. However, safety and efficacy should be investigated prior to clinical application. The objective of this study was to evaluate [...] Read more.
Background: Cell-Based Therapies (CBT) constitute a valid procedure for increasing the quantity and quality of bone in areas with an inadequate bone volume. However, safety and efficacy should be investigated prior to clinical application. The objective of this study was to evaluate the biodistribution, safety and osteogenic capacity of bone marrow-derived human mesenchymal stem cells (hBMMSCs) pre-seeded into β-tricalcium phosphate (TCP) and implanted into NOD/SCID mice at subcutaneous and intramuscular sites. Methods: hBMMSCs were isolated, characterized and then cultured in vitro on a porous β-TCP scaffold. Cell viability and attachment were analyzed and then hBMMSCs seeded constructs were surgically placed at subcutaneous and intramuscular dorsal sites into NOD/SCID mice. Acute and subchronic toxicity, cell biodistribution and efficacy were investigated. Results: There were no deaths or adverse events in treated mice during the 48-hour observation period, and no toxic response was observed in mice. In the 12-week subchronic toxicity study, no mortalities, abnormal behavioral symptoms or clinical signs were observed in the saline control mice or the hBMMSCs/β-TCP groups. Finally, our results showed the bone-forming capacity of hBMMSCs/β-TCP since immunohistochemical expression of human osteocalcin was detected from week 7. Conclusions: These results show that transplantation of hBMMSCs/β-TCP in NOD/SCID mice are safe and effective, and might be applied to human bone diseases in future clinical trials. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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8 pages, 1392 KiB  
Article
Calcium Charge and Release of Conventional Glass-Ionomer Cement Containing Nanoporous Silica
by Koichi Nakamura, Shigeaki Abe, Hajime Minamikawa and Yasutaka Yawaka
Materials 2018, 11(8), 1295; https://doi.org/10.3390/ma11081295 - 27 Jul 2018
Cited by 8 | Viewed by 3619
Abstract
The aim of this study was to evaluate calcium charge and release of conventional glass-ionomer cement (GIC) containing nanoporous silica (NPS). Experimental specimens were divided into two groups: the control (GIC containing no NPS) and GIC-NPS (GIC containing 10 wt % NPS). The [...] Read more.
The aim of this study was to evaluate calcium charge and release of conventional glass-ionomer cement (GIC) containing nanoporous silica (NPS). Experimental specimens were divided into two groups: the control (GIC containing no NPS) and GIC-NPS (GIC containing 10 wt % NPS). The specimens were immersed in calcium chloride solutions of 5 wt % calcium concentration for 24 h at 37 °C, whereupon the calcium ion release of the specimens was measured. The calcium ion release behavior of GIC-NPS after immersion in the calcium solution was significantly greater than that of the control. Scanning electron microscopy and electron-dispersive X-ray spectroscopy results indicated that calcium penetrated inside the GIC-NPS specimen, while the calcium was primarily localized on the surface of the control specimen. It was demonstrated that NPS markedly improved the calcium charge and release property of GIC. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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15 pages, 5009 KiB  
Article
Human Dental Pulp Stem Cells Exhibit Different Biological Behaviours in Response to Commercial Bleaching Products
by Carmen Llena, Mar Collado-González, Christopher Joseph Tomás-Catalá, David García-Bernal, Ricardo Elías Oñate-Sánchez, Francisco Javier Rodríguez-Lozano and Leopoldo Forner
Materials 2018, 11(7), 1098; https://doi.org/10.3390/ma11071098 - 27 Jun 2018
Cited by 10 | Viewed by 4626
Abstract
The purpose of this study was to evaluate the diffusion capacity and the biological effects of different bleaching products on human dental pulp stem cells (hDPSCs). The bleaching gel was applied for 90, 30 or 15 min to enamel/dentine discs that [...] Read more.
The purpose of this study was to evaluate the diffusion capacity and the biological effects of different bleaching products on human dental pulp stem cells (hDPSCs). The bleaching gel was applied for 90, 30 or 15 min to enamel/dentine discs that adapted in an artificial chamber. The diffusion of hydrogen peroxide (HP) was analysed by fluorometry and the diffusion products were applied to hDPSCs. Cell viability, cell migration and cell morphology assays were performed using the eluates of diffusion products. Finally, cell apoptosis and the expression of mesenchymal stem cell markers were analysed by flow cytometry. Statistical analysis was performed using analysis of variance and Kruskal–Wallis or Mann–Whitney tests (α < 0.05). Significant reductions of approximately 95% in cell viability were observed for the 3 × 15 min groups (p < 0.001), while 1 × 30 min of PerfectBleach and 1 × 90 min of PolaNight resulted in reductions of 50% and 60% in cell viability, respectively (p < 0.001). Similar results were obtained in the migration assay. Moreover, the 3 × 15 min group was associated with cell morphology alterations and reductions of >70% in cell live. Finally, hDPSCs maintained their mesenchymal phenotype in all conditions. Similar concentrations of carbamide peroxide (CP) and HP in different commercial products exhibited different biological effects on hDPSCs. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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13 pages, 1415 KiB  
Article
Physicochemical and Microbiological Assessment of an Experimental Composite Doped with Triclosan-Loaded Halloysite Nanotubes
by Diana A. Cunha, Nara S. Rodrigues, Lidiane C. Souza, Diego Lomonaco, Flávia P. Rodrigues, Felipe W. Degrazia, Fabrício M. Collares, Salvatore Sauro and Vicente P. A. Saboia
Materials 2018, 11(7), 1080; https://doi.org/10.3390/ma11071080 - 25 Jun 2018
Cited by 22 | Viewed by 5443
Abstract
This study is aimed at evaluating the effects of triclosan-encapsulated halloysite nanotubes (HNT/TCN) on the physicochemical and microbiological properties of an experimental dental composite. A resin composite doped with HNT/TCN (8% w/w), a control resin composite without nanotubes (HNT/TCN-0%) and [...] Read more.
This study is aimed at evaluating the effects of triclosan-encapsulated halloysite nanotubes (HNT/TCN) on the physicochemical and microbiological properties of an experimental dental composite. A resin composite doped with HNT/TCN (8% w/w), a control resin composite without nanotubes (HNT/TCN-0%) and a commercial nanofilled resin (CN) were assessed for degree of conversion (DC), flexural strength (FS), flexural modulus (FM), polymerization stress (PS), dynamic thermomechanical (DMA) and thermogravimetric analysis (TGA). The antibacterial properties (M) were also evaluated using a 5-day biofilm assay (CFU/mL). Data was submitted to one-way ANOVA and Tukey tests. There was no significant statistical difference in DC, FM and RU between the tested composites (p > 0.05). The FS and CN values attained with the HNT/TCN composite were higher (p < 0.05) than those obtained with the HNT/TCN-0%. The DMA analysis showed significant differences in the TAN δ (p = 0.006) and Tg (p = 0) between the groups. TGA curves showed significant differences between the groups in terms of degradation (p = 0.046) and weight loss (p = 0.317). The addition of HNT/TCN induced higher PS, although no significant antimicrobial effect was observed (p = 0.977) between the groups for CFUs and (p = 0.557) dry weight. The incorporation of HNT/TCN showed improvements in physicochemical and mechanical properties of resin composites. Such material may represent an alternative choice for therapeutic restorative treatments, although no significance was found in terms of antibacterial properties. However, it is possible that current antibacterial tests, as the one used in this laboratory study, may not be totally appropriate for the evaluation of resin composites, unless accompanied with aging protocols (e.g., thermocycling and load cycling) that allow the release of therapeutic agents incorporated in such materials. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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27 pages, 4460 KiB  
Article
Properties of Experimental Dental Composites Containing Antibacterial Silver-Releasing Filler
by Robert Stencel, Jacek Kasperski, Wojciech Pakieła, Anna Mertas, Elżbieta Bobela, Izabela Barszczewska-Rybarek and Grzegorz Chladek
Materials 2018, 11(6), 1031; https://doi.org/10.3390/ma11061031 - 18 Jun 2018
Cited by 44 | Viewed by 5966 | Correction
Abstract
Secondary caries is one of the important issues related to using dental composite restorations. Effective prevention of cariogenic bacteria survival may reduce this problem. The aim of this study was to evaluate the antibacterial activity and physical properties of composite materials with silver [...] Read more.
Secondary caries is one of the important issues related to using dental composite restorations. Effective prevention of cariogenic bacteria survival may reduce this problem. The aim of this study was to evaluate the antibacterial activity and physical properties of composite materials with silver sodium hydrogen zirconium phosphate (SSHZP). The antibacterial filler was introduced at concentrations of 1%, 4%, 7%, 10%, 13%, and 16% (w/w) into model composite material consisting of methacrylate monomers and silanized glass and silica fillers. The in vitro reduction in the number of viable cariogenic bacteria Streptococcus mutans ATCC 33535 colonies, Vickers microhardness, compressive strength, diametral tensile strength, flexural strength, flexural modulus, sorption, solubility, degree of conversion, and color stability were investigated. An increase in antimicrobial filler concentration resulted in a statistically significant reduction in bacteria. There were no statistically significant differences caused by the introduction of the filler in compressive strength, diametral tensile strength, flexural modulus, and solubility. Statistically significant changes in degree of conversion, flexural strength, hardness (decrease), solubility (increase), and in color were registered. A favorable combination of antibacterial properties and other properties was achieved at SSHZP concentrations from 4% to 13%. These composites exhibited properties similar to the control material and enhanced in vitro antimicrobial efficiency. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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12 pages, 2973 KiB  
Article
Modified Polymeric Nanoparticles Exert In Vitro Antimicrobial Activity Against Oral Bacteria
by Manuel Toledano-Osorio, Jegdish P. Babu, Raquel Osorio, Antonio L. Medina-Castillo, Franklin García-Godoy and Manuel Toledano
Materials 2018, 11(6), 1013; https://doi.org/10.3390/ma11061013 - 14 Jun 2018
Cited by 26 | Viewed by 3886
Abstract
Polymeric nanoparticles were modified to exert antimicrobial activity against oral bacteria. Nanoparticles were loaded with calcium, zinc and doxycycline. Ions and doxycycline release were measured by inductively coupled plasma optical emission spectrometer and high performance liquid chromatography. Porphyromonas gingivalis, Lactobacillus lactis, [...] Read more.
Polymeric nanoparticles were modified to exert antimicrobial activity against oral bacteria. Nanoparticles were loaded with calcium, zinc and doxycycline. Ions and doxycycline release were measured by inductively coupled plasma optical emission spectrometer and high performance liquid chromatography. Porphyromonas gingivalis, Lactobacillus lactis, Streptoccocus mutans, gordonii and sobrinus were grown and the number of bacteria was determined by optical density. Nanoparticles were suspended in phosphate-buffered saline (PBS) at 10, 1 and 0.1 mg/mL and incubated with 1.0 mL of each bacterial suspension for 3, 12, and 24 h. The bacterial viability was assessed by determining their ability to cleave the tetrazolium salt to a formazan dye. Data were analyzed by ANOVA and Scheffe’s F (p < 0.05). Doxycycline doping efficacy was 70%. A burst liberation effect was produced during the first 7 days. After 21 days, a sustained release above 6 µg/mL, was observed. Calcium and zinc liberation were about 1 and 0.02 µg/mL respectively. The most effective antibacterial material was found to be the Dox-Nanoparticles (60% to 99% reduction) followed by Ca-Nanoparticles or Zn-Nanoparticles (30% to 70% reduction) and finally the non-doped nanoparticles (7% to 35% reduction). P. gingivalis, S. mutans and L. lactis were the most susceptible bacteria, being S. gordonii and S. sobrinus the most resistant to the tested nanoparticles. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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15 pages, 4062 KiB  
Article
Dental Resin Cements—The Influence of Water Sorption on Contraction Stress Changes and Hydroscopic Expansion
by Grzegorz Sokolowski, Agata Szczesio, Kinga Bociong, Karolina Kaluzinska, Barbara Lapinska, Jerzy Sokolowski, Monika Domarecka and Monika Lukomska-Szymanska
Materials 2018, 11(6), 973; https://doi.org/10.3390/ma11060973 - 08 Jun 2018
Cited by 45 | Viewed by 6259
Abstract
Resin matrix dental materials undergo contraction and expansion changes due to polymerization and water absorption. Both phenomena deform resin-dentin bonding and influence the stress state in restored tooth structure in two opposite directions. The study tested three composite resin cements (Cement-It, NX3, Variolink [...] Read more.
Resin matrix dental materials undergo contraction and expansion changes due to polymerization and water absorption. Both phenomena deform resin-dentin bonding and influence the stress state in restored tooth structure in two opposite directions. The study tested three composite resin cements (Cement-It, NX3, Variolink Esthetic DC), three adhesive resin cements (Estecem, Multilink Automix, Panavia 2.0), and seven self-adhesive resin cements (Breeze, Calibra Universal, MaxCem Elite Chroma, Panavia SA Cement Plus, RelyX U200, SmartCem 2, and SpeedCEM Plus). The stress generated at the restoration-tooth interface during water immersion was evaluated. The shrinkage stress was measured immediately after curing and after 0.5 h, 24 h, 72 h, 96 h, 168 h, 240 h, 336 h, 504 h, 672 h, and 1344 h by means of photoelastic study. Water sorption and solubility were also studied. All tested materials during polymerization generated shrinkage stress ranging from 4.8 MPa up to 15.1 MPa. The decrease in shrinkage strain (not less than 57%) was observed after water storage (56 days). Self-adhesive cements, i.e., MaxCem Elite Chroma, SpeedCem Plus, Panavia SA Plus, and Breeze exhibited high values of water expansion stress (from 0 up to almost 7 MPa). Among other tested materials only composite resin cement Cement It and adhesive resin cement Panavia 2.0 showed water expansion stress (1.6 and 4.8, respectively). The changes in stress value (decrease in contraction stress or built up of hydroscopic expansion) in time were material-dependent. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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13 pages, 9163 KiB  
Article
Effects of Three Calcium Silicate Cements on Inflammatory Response and Mineralization-Inducing Potentials in a Dog Pulpotomy Model
by Chung-Min Kang, Jiwon Hwang, Je Seon Song, Jae-Ho Lee, Hyung-Jun Choi and Yooseok Shin
Materials 2018, 11(6), 899; https://doi.org/10.3390/ma11060899 - 27 May 2018
Cited by 10 | Viewed by 4927
Abstract
This beagle pulpotomy study compared the inflammatory response and mineralization-inducing potential of three calcium silicate cements: ProRoot mineral trioxide aggregate (MTA) (Dentsply, Tulsa, OK, USA), OrthoMTA (BioMTA, Seoul, Korea), and Endocem MTA (Maruchi, Wonju, Korea). Exposed pulp tissues were capped with ProRoot MTA, [...] Read more.
This beagle pulpotomy study compared the inflammatory response and mineralization-inducing potential of three calcium silicate cements: ProRoot mineral trioxide aggregate (MTA) (Dentsply, Tulsa, OK, USA), OrthoMTA (BioMTA, Seoul, Korea), and Endocem MTA (Maruchi, Wonju, Korea). Exposed pulp tissues were capped with ProRoot MTA, OrthoMTA, or Endocem MTA. After 8 weeks, we extracted the teeth, then performed hematoxylin-eosin and immunohistochemical staining with osteocalcin and dentin sialoprotein. Histological evaluation comprised a scoring system with eight broad categories and analysis of calcific barrier areas. We evaluated 44 teeth capped with ProRoot MTA (n = 15), OrthoMTA (n = 18), or Endocem MTA (n = 11). Most ProRoot MTA specimens formed continuous calcific barriers; these pulps contained inflammation-free palisading patterns in the odontoblastic layer. Areas of the newly formed calcific barrier were greater with ProRoot MTA than with Endocem MTA (p = 0.006). Although dentin sialoprotein was highly expressed in all three groups, the osteocalcin expression was reduced in the OrthoMTA and Endocem MTA groups. ProRoot MTA was superior to OrthoMTA and Endocem MTA in all histological analyses. ProRoot MTA and OrthoMTA resulted in reduced pulpal inflammation and more complete calcific barrier formation, whereas Endocem MTA caused a lower level of calcific barrier continuity with tunnel defects. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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15 pages, 3107 KiB  
Article
Antibacterial, Hydrophilic Effect and Mechanical Properties of Orthodontic Resin Coated with UV-Responsive Photocatalyst
by Akira Kuroiwa, Yoshiaki Nomura, Tsuyoshi Ochiai, Tomomi Sudo, Rie Nomoto, Tohru Hayakawa, Hiroyuki Kanzaki, Yoshiki Nakamura and Nobuhiro Hanada
Materials 2018, 11(6), 889; https://doi.org/10.3390/ma11060889 - 25 May 2018
Cited by 16 | Viewed by 4377
Abstract
Photocatalysts have multiple applications in air purifiers, paints, and self-cleaning coatings for medical devices such as catheters, as well as in the elimination of xenobiotics. In this study, a coating of a UV-responsive photocatalyst, titanium dioxide (TiO2), was applied to an [...] Read more.
Photocatalysts have multiple applications in air purifiers, paints, and self-cleaning coatings for medical devices such as catheters, as well as in the elimination of xenobiotics. In this study, a coating of a UV-responsive photocatalyst, titanium dioxide (TiO2), was applied to an orthodontic resin. The antibacterial activity on oral bacteria as well as hydrophilic properties and mechanical properties of the TiO2-coated resin were investigated. ultraviolet A (UVA) (352 nm) light was used as the light source. Antibacterial activity was examined with or without irradiation. Measurements of early colonizers and cariogenic bacterial count, i.e., colony forming units (CFU), were performed after irradiation for different time durations. Hydrophilic properties were evaluated by water contact angle measurements. While, for the assessment of mechanical properties, flexural strength was measured by the three-point bending test. In the coat(+)light(+) samples the CFU were markedly decreased compared to the control samples. Water contact angle of the coat(+)light(+) samples was decreased after irradiation. The flexural strength of the specimen irradiated for long time showed a higher value than the required standard value, indicating that the effect of irradiation was weak. We suggest that coating with the ultraviolet responsive photocatalyst TiO2 is useful for the development of orthodontic resin with antimicrobial properties. Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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2 pages, 473 KiB  
Correction
Correction: Stencel, R., et al. Properties of Experimental Dental Composites Containing Antibacterial Silver-Releasing Filler. Materials 2018, 11, 1031
by Robert Stencel, Jacek Kasperski, Wojciech Pakieła, Anna Mertas, Elżbieta Bobela, Izabela Barszczewska-Rybarek and Grzegorz Chladek
Materials 2018, 11(11), 2173; https://doi.org/10.3390/ma11112173 - 02 Nov 2018
Cited by 11 | Viewed by 2087
Abstract
In the published article, “Properties of Experimental Dental Composites Containing Antibacterial Silver-Releasing Filler” [...] Full article
(This article belongs to the Special Issue Bioactive and Therapeutic Dental Materials)
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