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Microarrays
Special Issues
Microarrays for Food Safety and Quality
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Microarrays for Food Safety and Quality

A special issue of Microarrays (ISSN 2076-3905).

Deadline for manuscript submissions: closed (31 March 2016) | Viewed by 6193

Special Issue Editor

Dr. Marcos Alcocer

E-Mail Website
Guest Editor
School of Biosciences, University of Nottingham, Sutton Bonington campus, Loughborough, LE12 5RD, UK
Interests: allergen, food allergen, adaptive and innate immunology (basophil, NKT, DC), mechanisms of allergeniciy, protein expression, protein structure

Special Issue Information

Dear Colleagues,

Within the last few years the microarray technologies have matured and moved from specialized and well-equipped laboratories into a worldwide user scale. From the original DNA/RNA surface approach to new formats involving peptides, proteins, complex lipids, carbohydrates, intact cells amongst others and single or multiplex detection systems, such as PNA, RNA, DNA, antibodies, fluorochromes, enzymes, cells or SPR (surface plasmon resonance) the microarray technologies are changing the analytical field in many areas. In this Special Issue, we are inviting manuscripts on the successful use of microarrays in the important area of food safety and quality. Hence, microarray work on the food areas of drug resistance, drug residues, adulteration (including parasites and GMO), authentication, pathogen (microbial, fungal, or viral) detection, allergens characterization or work on nutraceuticals amongst other related topics (in any platform) are welcome. It is our intention to reflect, in this Special Issue, the great contribution these versatile, multi purpose, high throughput technologies are making to the area of food safety and quality.

Dr. Marcos Alcocer
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microarrays is an international peer-reviewed open access quarterly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 350 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • drug resistance
  • drug residues
  • food adulteration
  • food parasites
  • GMO
  • food authentication
  • food pathogen
  • food borne bacteria
  • food borne fungi
  • food born virus
  • allergens
  • nutraceuticals

Published Papers (1 paper)

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Review

1695 KiB  
Review
Use of a Pan–Genomic DNA Microarray in Determination of the Phylogenetic Relatedness among Cronobacter spp. and Its Use as a Data Mining Tool to Understand Cronobacter Biology
by Ben D. Tall, Jayanthi Gangiredla, Christopher J. Grim, Isha R. Patel, Scott A. Jackson, Mark K. Mammel, Mahendra H. Kothary, Venugopal Sathyamoorthy, Laurenda Carter, Séamus Fanning, Carol Iversen, Franco Pagotto, Roger Stephan, Angelika Lehner, Jeffery Farber, Qiong Q. Yan and Gopal R. Gopinath
Microarrays 2017, 6(1), 6; https://doi.org/10.3390/microarrays6010006 - 04 Mar 2017
Cited by 5 | Viewed by 5802
Abstract
Cronobacter (previously known as Enterobacter sakazakii) is a genus of Gram-negative, facultatively anaerobic, oxidase-negative, catalase-positive, rod-shaped bacteria of the family Enterobacteriaceae. These organisms cause a variety of illnesses such as meningitis, necrotizing enterocolitis, and septicemia in neonates and infants, and urinary [...] Read more.
Cronobacter (previously known as Enterobacter sakazakii) is a genus of Gram-negative, facultatively anaerobic, oxidase-negative, catalase-positive, rod-shaped bacteria of the family Enterobacteriaceae. These organisms cause a variety of illnesses such as meningitis, necrotizing enterocolitis, and septicemia in neonates and infants, and urinary tract, wound, abscesses or surgical site infections, septicemia, and pneumonia in adults. The total gene content of 379 strains of Cronobacter spp. and taxonomically-related isolates was determined using a recently reported DNA microarray. The Cronobacter microarray as a genotyping tool gives the global food safety community a rapid method to identify and capture the total genomic content of outbreak isolates for food safety, environmental, and clinical surveillance purposes. It was able to differentiate the seven Cronobacter species from one another and from non-Cronobacter species. The microarray was also able to cluster strains within each species into well-defined subgroups. These results also support previous studies on the phylogenic separation of species members of the genus and clearly highlight the evolutionary sequence divergence among each species of the genus compared to phylogenetically-related species. This review extends these studies and illustrates how the microarray can also be used as an investigational tool to mine genomic data sets from strains. Three case studies describing the use of the microarray are shown and include: (1) the determination of allelic differences among Cronobacter sakazakii strains possessing the virulence plasmid pESA3; (2) mining of malonate and myo-inositol alleles among subspecies of Cronobacter dublinensis strains to determine subspecies identity; and (3) lastly using the microarray to demonstrate sequence divergence and phylogenetic relatedness trends for 13 outer-membrane protein alleles among 240 Cronobacter and phylogenetically-related strains. The goal of this review is to describe microarrays as a robust tool for genomics research of this assorted and important genus, a criterion toward the development of future preventative measures to eliminate this foodborne pathogen from the global food supply. Full article
(This article belongs to the Special Issue Microarrays for Food Safety and Quality)
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