Special Issue "Phage Display of Combinatorial Libraries"
A special issue of Molecules (ISSN 1420-3049).
Deadline for manuscript submissions: closed (15 December 2010)
Dr. Brendan Patrick Orner (Website)
Division of Chemistry & Biological Chemistry, Nanyang Technological University, 637371, Singapore
Interests: phage display; protein-protein interactions; chemical biology; nanomaterials; combinatorial techniques; sugar mimics; ferritin; protein folding; biomineralization
Phage display is a combinatorial molecular biology technique where libraries (commonly peptides and proteins) are fused to assembled bacteriophage scaffolds. The viability of the phage affords the technique advantages over those based on small molecules. The libraries can be amplified simply by infecting bacteria, and because the genome of the phage is encapsulated within the viron, DNA sequencing can be employed to easily deconvolute the libraries. In addition, because diversity is controlled by the synthesis of degenerate DNA, libraries with orders of magnitude more members than those using small molecules can be achieved. Moreover the generation of phage display libraries can be performed in any lab with basic microbiology facilities, and it is therefore extremely cheap compared to small-molecule library synthesis. Due to its power, versatility, ease of use, and low cost phage display has been widely used and incorporated into a variety of applications.
This special issue of Molecules will consider any submission associated with phage display whether it be its use for discovering novel ligands, the development of new phage display techniques, or its utilization in novel applications.
Dr. Brendan P. Orner
- phage display
- combinatorial molecular biology
- combinatorial libraries
- in vitro selection