Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
2.5
2.6
Journals
Separations
Special Issues
Countercurrent Chromatography
share announcement

Countercurrent Chromatography

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: closed (20 August 2017) | Viewed by 23027

Special Issue Editor

Prof. Dr. Alain Berthod

E-Mail Website
Guest Editor
Institut des Sciences Analytiques, Université de Lyon, CNRS, 5 rue de la Doua, 69100 Villeurbanne, France
Interests: stationary phase; ionic liquid; liquid chromatography; partition coefficient; countercurrent chromatography and centrifugal partition chromatography

Special Issue Information

Dear Colleagues,

The Special Issue of Separations, dedicated to countercurrent chromatography (CCC), will be equally review and research oriented with articles, presenting the significant advancements made in the CCC technique this century. It will focus on the recent trend in separation and purification made using support-free liquid phases. Reliable CCC equipment, new biphasic liquid systems, different ways to purify, to extract and/or to separate components in chemical mixtures, industrial CCC uses, applications in natural products, food, and chemical or pharmaceutical industries will all be treated in the Special Issue. All CCC works, either fundamental or applied, are equally welcome.

Prof. Dr. Alain Berthod
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • countercurrent chromatography
  • centrifugal partition chromatography
  • industrial uses
  • natural products
  • purification
  • preparative chromatography
  • large scale separation
  • scaling-up
  • biphasic liquid systems
  • traditional Chinese medicines
  • green processes

Published Papers (5 papers)

Download All Papers
Order results
Result details
Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:

Editorial

Jump to: Research

2111 KiB  
Editorial
Separation and Purification with a Liquid Stationary Phase
by Alain Berthod
Separations 2017, 4(4), 30; https://doi.org/10.3390/separations4040030 - 17 Oct 2017
Cited by 2 | Viewed by 3536
Abstract
Yoichiro Ito introduced countercurrent chromatography (CCC) in 1966, reporting the separation of blood plasma cells with a sealed helical tube. Since then, CCC has been a fertile ground for instrumental and technical innovation. The key innovation of CCC was to use centrifugal forces [...] Read more.
Yoichiro Ito introduced countercurrent chromatography (CCC) in 1966, reporting the separation of blood plasma cells with a sealed helical tube. Since then, CCC has been a fertile ground for instrumental and technical innovation. The key innovation of CCC was to use centrifugal forces to retain the stationary liquid phase in the column in such a way that it is able to interact dynamically with the mobile phase without any solid support. The broad diversity of countercurrent separation terminology reflects the innovative spirit of the field, as well as the global appeal of this technique. The selection of the appropriate biphasic liquid system is the core of the CCC technique. The CCC columns must generate the centrifugal field needed to maintain the liquid stationary phase; therefore, they cannot be a simple tube with frits at both ends. Rotors, motor, gears, spools, and rotating seals are very specific things that are not needed in a classical liquid chromatography column with a solid stationary phase. The differences between the two main types of CCC columns are described. The bases of the CCC theory are also given. Full article
(This article belongs to the Special Issue Countercurrent Chromatography)
►▼ Show Figures

Figure 1

Research

Jump to: Editorial

1976 KiB  
Article
Dispersed Mobile-Phase Countercurrent Chromatography
by Timothy Yiu-Cheong Ho and Hong Xue
Separations 2016, 3(4), 32; https://doi.org/10.3390/separations3040032 - 01 Nov 2016
Viewed by 6741
Abstract
Countercurrent distribution based on liquid–liquid partition is a powerful separation method with minimal incurrence of loss of solutes, but its industrial application has been limited by cumbersome shifting of immiscible solvents. Although centrifugation has been employed to facilitate equilibration between phases, process scaling-up [...] Read more.
Countercurrent distribution based on liquid–liquid partition is a powerful separation method with minimal incurrence of loss of solutes, but its industrial application has been limited by cumbersome shifting of immiscible solvents. Although centrifugation has been employed to facilitate equilibration between phases, process scaling-up remains difficult. In this study, a dispersed mobile-phase countercurrent chromatography (DMCC) method has been developed to adapt the countercurrent distribution principle to a continuous column chromatography format. Continuous solute exchange between two immiscible phases within a series of separation columns is achieved by mechanical dispersion of an influx of mobile phase into an upward stream of small droplets travelling through the columns filled with stationary phase. The diameter, length, and number of columns, and the number of stationary phases employed in the different columns can be varied to match the requisite scale and resolution of operation. Illustrations of DMCC were provided by examples of solute separations where the fractionated solutes could be collected either from the eluate of the series of columns, or from drainage of the stationary phases in the individual columns at the end of a chromatographic run. Full article
(This article belongs to the Special Issue Countercurrent Chromatography)
►▼ Show Figures

Figure 1

948 KiB  
Article
Comparison in Partition Efficiency of Protein Separation between Four Different Tubing Modifications in Spiral High-Speed Countercurrent Chromatography
by Yoichiro Ito and Robert Clary
Separations 2016, 3(4), 31; https://doi.org/10.3390/separations3040031 - 14 Oct 2016
Cited by 9 | Viewed by 3379
Abstract
High-speed countercurrent chromatography with a spiral tube assembly can retain a satisfactory amount of stationary phase of polymer phase systems used for protein separation. In order to improve the partition efficiency, a simple tool was fabricated to modify the tubing shapes, and the [...] Read more.
High-speed countercurrent chromatography with a spiral tube assembly can retain a satisfactory amount of stationary phase of polymer phase systems used for protein separation. In order to improve the partition efficiency, a simple tool was fabricated to modify the tubing shapes, and the following four different tubing modifications were made: intermittently pressed at 10 mm width, flat, flat-wave, and flat-twist. Partition efficiencies of the separation column made from these modified tubings were examined in protein separation with an aqueous–aqueous polymer phase system at flow rates of 1–2 mL/min under 800 rpm. The results indicated that the column with all modified tubing improved the partition efficiency at a flow rate of 1 mL/min, but at a higher flow rate of 2 mL/min, the columns made of flattened tubing showed lowered partition efficiency, apparently due to the loss of the retained stationary phase. Among all the modified columns, the column with intermittently pressed tubing gave the best peak resolution. It may be concluded that the intermittently pressed and flat-twist improve the partition efficiency in a semi-preparative separation, while other modified tubing of flat and flat-wave configurations may be used for analytical separations with a low flow rate. Full article
(This article belongs to the Special Issue Countercurrent Chromatography)
►▼ Show Figures

Figure 1

2378 KiB  
Article
Improved Separations of Proteins and Sugar Derivatives Using the Small-Scale Cross-Axis Coil Planet Centrifuge with Locular Multilayer Coiled Columns
by Kazufusa Shinomiya, Kazumasa Zaima, Naoki Harikai and Yoichiro Ito
Separations 2016, 3(4), 29; https://doi.org/10.3390/separations3040029 - 22 Sep 2016
Cited by 3 | Viewed by 3858
Abstract
(1) Background: Countercurrent chromatography (CCC) is liquid-liquid partition chromatography without using a solid support matrix. This technique requires further improvement of peak resolution and shortening of separation time. (2) Methods: The long-pressed locular multilayer coils with and without mixer glass beads were developed [...] Read more.
(1) Background: Countercurrent chromatography (CCC) is liquid-liquid partition chromatography without using a solid support matrix. This technique requires further improvement of peak resolution and shortening of separation time. (2) Methods: The long-pressed locular multilayer coils with and without mixer glass beads were developed for the separation of proteins and 4-methylumbelliferyl sugar derivatives using a small-scale cross-axis coil planet centrifuge. (3) Results: Proteins were separated from each other and the separation was improved when the flow rate of the mobile phase was decreased from 0.8 to 0.4 mL/min. On the other hand, 4-methylumbelliferyl sugar derivatives were separated at the resolution of almost over 1.5 in short separation time under satisfactory stationary phase retention when the flow rate of the mobile phase was increased from 1.0 to 1.4 mL/min. (4) Conclusion: Better peak resolutions over the previous results were achieved using the long-pressed locular multilayer coil for proteins with aqueous two-phase systems (ATPS) and for 4-methylumbelliferyl sugar derivatives with organic-aqueous two-phase solvent systems by inserting a glass bead into each locule. Full article
(This article belongs to the Special Issue Countercurrent Chromatography)
►▼ Show Figures

Graphical abstract

2349 KiB  
Article
Gram-Scale Purification of Dihydrorobinetin from Robinia pseudoacacia L. Wood by Centrifugal Partition Chromatography
by Emilie Destandau, Jean-Paul Charpentier, Stéphane Bostyn, Sandrine Zubrzycki, Valérie Serrano, Jean-Marc Seigneuret and Christian Breton
Separations 2016, 3(3), 23; https://doi.org/10.3390/separations3030023 - 11 Aug 2016
Cited by 13 | Viewed by 4835
Abstract
Robinia pseudoacacia L. is a tree widely dispersed in France that is characterized by good growth rates and important biomass production, which produces wood with very high natural durability used for outdoor fence posts, timber, and barrels to age vinegars and wines. Its [...] Read more.
Robinia pseudoacacia L. is a tree widely dispersed in France that is characterized by good growth rates and important biomass production, which produces wood with very high natural durability used for outdoor fence posts, timber, and barrels to age vinegars and wines. Its mature heartwood presents high resistance against wood fungi decay and contains two main flavonoid extractives, dihydrorobinetin—the most abundant—and robinetin that present interesting biological activities. The aim of the present study was to optimize a procedure allowing an important recovery of purified dihydrorobinetin from R. pseudoacacia wood, representing an interesting sustainable, local, highly available, and, consequently, economical source of bioactive components. The extraction of dihydrorobinetin was first optimized by evaluating the influence of various extraction parameters such as temperature, extraction time, solvent nature, and wood/solvent mass ratio to obtain an efficient, safe, and low cost extraction. Then, dihydrorobinetin was purified over 95% using centrifugal partition chromatography (CPC). CPC purification was first developed on a small volume column with low amounts of injected extract, then scaled-up on a 200 mL column with higher sample loading capacity in order to purify more than 1.3 g of dihydrorobinetin in one run. Full article
(This article belongs to the Special Issue Countercurrent Chromatography)
►▼ Show Figures

Graphical abstract

Show export options expand_more Show export options expand_less
Select all
Export citation of selected articles as:
 
Displaying articles 1-5
Back to TopTop