Seeds doi: 10.3390/seeds5040048

Authors: István Kristó Attila Rácz Péter Jakab Mária Ágnes Fodor

The species that make up the intercrop interact with each other during their development, which can be beneficial, harmful, or indifferent to the participants. The aim of this study was to determine whether the developmental differences observed between intercropped and pure stands are already evident at early developmental stages. We conducted three experiments: (1) a laboratory germination test using filter paper rolls, (2) a laboratory growth test in plant pots, and (3) a small-plot field experiment comparing the development of mixtures and monocultures of winter cereals (winter wheat, winter durum wheat, winter einkorn, winter triticale, winter barley, and winter oats) and winter peas. Based on our results, only minor differences were observed (1–3%) in germination parameters (germination percentage, germination vigor, and seedling health). However, as plant development progressed, increasingly pronounced differences emerged (significance level of p < 0.05) between intercropped and pure stands based on which we assume allelopathic effects. Winter wheat and durum wheat had a positive effect on the initial development of winter pea, whereas barley, and oat had a clearly negative effect. Winter pea negatively affected the germination and early development of triticale and oat, while it had a positive effect on the initial development of durum wheat. Mutually beneficial interactions were identified in the winter wheat–pea and durum wheat–pea combinations during the early stages of plant association.

Seeds doi: 10.3390/seeds5040047

Authors: Wannalak Senagul Areeya Mungsachat Salisa Suchitwarasan

Bacterial isolates obtained from spores of the arbuscular mycorrhizal fungi (AMF) have been reported to possess various plant growth-promoting traits. These bacterial isolates enhance plant growth and development through mechanisms such as phytohormone production and nutrient mobilization. The objective of this study was to investigate the ability of bacterial isolates to promote plant development. Five bacterial isolates, Bacillus methylotrophicus, Fictibacillus barbaricus, Stutzerimonas stutzeri, Bacillus aryabhattai, and Priestia aryabhattai, were isolated from AMF (Funneliformis mosseae) spores. The results indicated that all five bacterial isolates promoted plant growth. The greatest positive effects on the tomato seed germination were obtained with Bacillus methylotrophicus and Stutzerimonas stutzeri, with germination percentages of 90% and 95%, respectively. In addition, Stutzerimonas stutzeri enhanced early tomato seedling growth. Priestia aryabhattai produced the highest amount of indole-3-acetic acid (IAA) at 94.43 µg mL−1, which is the auxin phytohormone for plant growth promotion. Furthermore, Bacillus methylotrophicus, Fictibacillus barbaricus, and Bacillus aryabhattai were able to produce high levels of ammonia, which may contribute to improving plant growth in nutrient-deficient environments. In addition, Bacillus methylotrophicus, Bacillus aryabhattai, and Priestia aryabhattai were able to solubilize both phosphate and potassium. These findings suggest that the investigated bacterial strains could be combined with AMF to improve crop establishment and support sustainable agricultural systems by enhancing nutrient availability and reducing dependence on chemical fertilizers.

Seeds doi: 10.3390/seeds5040046

Authors: Maihe Ren Shuqi Li Chenjie Wei Yangqing Ou Weiran Dai Jian Ren

Despite the potential of Eragrostis nigra in alleviating forage shortages and controlling soil erosion, there is little information about how nitrogen fertilization and row spacing affect its forage and seed production. Thus, a two-year field experiment was conducted to explore the effects of nitrogen (N) fertilization (0, 45, 90, 135 kg hectare−1 (ha−1)) and row spacing (20 cm, 40 cm) on its forage yield, nutritive value, seed yield and quality. Results showed that forage dry matter yield (DMY) and harvested seed yield (HSY) increased with higher N rates; however, no further enhancement was observed at 135 kg per ha−1, making 90 kg ha−1 optimum. This may be because excessive nitrogen disrupts the carbon–nitrogen metabolic balance. N fertilization increased the relative feed value by enhancing crude protein content and decreasing acid detergent fiber. Relative to the 20 cm spacing, the 40 cm spacing averagely raised PSY and HSY by 34.7% and 21.3%, respectively. The quadratic regression model yielded maximum predicted HSY values of 27.6 and 26.7 kg ha−1, achieved at optimal N rates of 135 and 98.6 kg ha−1 for the 20 cm and 40 cm spacings, respectively. Thousand-seed weight (TSW), germination index, and vigor index were significantly improved by row spacing and N application (p < 0.05). Seed yield enhancement from N was positively correlated with increases in number of fertile tillers (FT), number of spikelets per tiller and seed set (p < 0.05). Furthermore, FT and TSW had the strongest direct effects on seed yield, with standardized path coefficients of 0.362 and 0.390. Our findings highlight that an application rate of 90 kg ha−1 N combined with 40 cm row spacing could be recommended as the efficient agronomic practice for maximizing forage yield, nutritive value, and seed yield in E. nigra. Importantly, this regime also enhances economic returns while reducing N leaching risks, thereby supporting more sustainable forage production systems.

Seeds doi: 10.3390/seeds5040045

Authors: Bin Wen Ya Zhang Xuejing Yin Ligang Chen

Germination traits and high-temperature tolerance are critical seed traits for species to respond to habitat changes through seed germination and population dynamics in tropical areas. This study investigated germination under temperature, water and NaCl stress and the seed high-temperature tolerance of three species growing in Xishuangbanna, i.e., one native vine species, Clematis subumbellata, and two introduced tree species, Handroanthus chrysanthus and Melaleuca viminalis. The results were compared with our previous studies in this region to assess the threatened status of C. subumbellata and invasion risk of H. chrysanthus and M. viminalis, in combination with their other traits. These three species are all characterized by the production of numerous small seeds with high viability every year, having the ability to increase their population rapidly under favorable conditions, though they all suffered viability loss and germination inhibition under high temperature and water stresses. The two introduced species, both H. chrysanthus and M. viminalis, produced seeds with wider germination temperature ranges and better high-temperature tolerance than the native C. subumbellata, but their seed heat tolerance was markedly lower than that of typical invasive weeds in this region. On the contrary, C. subumbellata seeds exhibited rather poor high-temperature tolerance, comparable to some rare and endangered species reported previously in the region; however, this species primarily grows on forest margins, slopes, and scrub and produces plumose achenes with good wind dispersal capacity. Thus, it was concluded that H. chrysanthus and M. viminalis may possess only low-to-moderate invasion risks, and their most probable invasive habitats are mainly forest edges. C. subumbellata can be regarded as not threatened currently, without urgent conservation demand. This information is useful for biodiversity conservation in Xishuangbanna.

Seeds doi: 10.3390/seeds5040044

Authors: María Quílez Gustavo J. Cáceres-Cevallos Pedro Sánchez-Gómez María J. Jordán

Salvia lavandulifolia Vahl. is an aromatic and medicinal plant cultivated as a rainfed crop for essential oil production in the western Mediterranean. New trends in the use of Lamiaceae seeds as functional ingredients warrant characterization of this species. Hence, studies were conducted to measure the proximate composition and amino acid profile, along with fatty acid profile, tocochromanol content, phenolic composition, and antioxidant activity in 10 wild populations. Defatted seed meal analysis demonstrates the potential usefulness of this species based on carbohydrate (50–60%), protein (13–21%), and oil (13–20%) content. The amino acid content ranged from 9.2 to 15.4 g/100 g. Seed oil analysis found a fatty acid content of 73–89 g/100 g with predominance of ω6 fatty acids, tocochromanol content of 62–89 mg/100 g, and a phenolic fraction of 7.5–148 mg/100 g with a high proportion of flavones. Antioxidant activity ranged from 10.6 to 23.1 µmol Trolox equivalents/100 g. Overall, seeds of S. lavandulifolia grown in subhumid conditions have strong nutritional and phytochemical profiles. These characteristics suggest opportunities for their use as functional ingredients. Further, strategies to minimize waste and maximize the availability of bioactive compounds will support the economic sustainability of this crop.

Seeds doi: 10.3390/seeds5040043

Authors: Anny Nogueira Fraga Josinei Rodrigues Filho Marina Reis Pires Viviana Borges Corte Hildegardo Seibert França

Solanum lycopersicum L. (tomato) is an economically and nutritionally important crop whose germination and early seedling growth are highly sensitive to abiotic stresses that disrupt physiological processes and intensify oxidative damage. Seed priming has emerged as a low-cost physiological preconditioning strategy to enhance stress tolerance by activating repair and defense mechanisms prior to stress exposure. This study evaluated the effects of osmopriming (PEG 6000), halopriming (KNO3), and chemopriming (SNP) on germination, early seedling growth, antioxidant enzyme activity, and lipid peroxidation in S. lycopersicum under salt stress and iron toxicity. Salt stress was induced with NaCl (100 mM) and iron toxicity with Fe-EDTA (4 mM). Priming treatments included osmopriming with PEG 6000 (−0.4 and −0.8 MPa), halopriming with KNO3 (25 and 50 mM), and chemopriming with sodium nitroprusside—SNP (0.1 and 0.2 mM). Under salt stress, NaCl (100 mM) completely inhibited germination and increased CAT activity by approximately 49% and POX activity by 4%; however, this antioxidant response was insufficient to reduce MDA content, which remained approximately 58% higher than the control. Under iron toxicity, Fe-EDTA (4 mM) reduced germination to 79% and caused a 425% increase in MDA content relative to the control, indicating severe oxidative damage despite a 59% increase in CAT and 47% increase in POX activities. Among the priming treatments evaluated, halopriming with KNO3 (50 mM) was the most effective strategy under salt stress, restoring germination to 81%, increasing CAT activity by 103%, and reducing MDA by 18% relative to non-primed salt-stressed seeds. Under iron toxicity, all priming treatments consistently increased antioxidant enzyme activities; however, none were capable of reducing lipid peroxidation or restoring seedling growth to control levels, likely due to continuous ROS generation via Fenton-type reactions that overwhelmed enzymatic detoxification capacity.

Seeds doi: 10.3390/seeds5040042

Authors: Antônio de Azevedo Perleberg Julio Cesar Paes Jacome de Araújo Filho Thaís Monteiro Miranda Taís Amanda Mundt Antonio Costa de Oliveira Luciano Carlos da Maia Camila Pegoraro Vívian Ebeling Viana

Background: Wheat (Triticum aestivum L.) seedling performance under heat and drought stress is critical for crop establishment. Identifying cultivars with superior seedling vigor under stress conditions helps breeding programs and seed production systems. Methods: We aimed to evaluate the early growth performance of wheat seedlings from 28 Brazilian commercial cultivars under heat and osmotic stress. Seedlings were assessed through shoot and root lengths and dry weight and analyzed using Scott–Knott grouping, heritability estimates, PCA and stress tolerance index. Results: Osmotic stress affected a wider range of traits than heat stress and revealed phenotypic and genetic variability among cultivars. Root length and root dry weight exhibited high broad-sense heritability, indicating a substantial genotypic contribution under the tested conditions and supporting their use for early-stage screening. Multivariate analyses revealed contrasting adaptation strategies, including root elongation and preferential allocation of biomass to roots under stress conditions. Through uni- and multivariate analyses, we suggested cultivars are potentially tolerant to heat and osmotic stresses. Conclusions: Root-related traits proved to be the most informative indicators of adaptation to stress, and the integration of stress tolerance indices and multivariate analyses enabled the identification of promising cultivars for further validation under greenhouse and field conditions.

Seeds doi: 10.3390/seeds5040041

Authors: Shillah Kwikiiriza Gail R. Nonnecke A. Susana Goggi David G. Acker

Limited access to quality seed constrains vegetable productivity and food security in Uganda, where informal seed systems remain central to farmers’ access to seed but are inadequately documented and largely unregulated. This study documented vegetable seed production and management practices, identified opportunities and constraints within informal seed systems, and examined the role of community seed banks in strengthening seed security. Using a mixed-method approach, quantitative surveys and key informant interviews were conducted with seed producers, seed traders, and community seed bank leaders across Uganda. Study respondents were predominantly men, with most respondents aged ≥35 years, despite women’s and youth’s primary role in vegetable production. Respondents reported key seed management constraints of seed storage pests, low-quality seeds, inconsistent market supply and price fluctuations, limited capital, and weak regulatory oversight. Seed treatment practices primarily consisted of sun-drying and plant-based pesticides, while seed quality assessment relied largely on experience and visual inspection, using cues such as seed color, size, and cleanliness, with limited use of standardized testing. Given that most vegetable growers obtain seeds through community-based seed channels, strengthening actors’ capacity to produce and supply high-quality seed is essential for sustaining vegetable production, improving farmer incomes, and enhancing food and nutrition security.

Seeds doi: 10.3390/seeds5040040

Authors: Simon J. Brooks Faiz F. Bebawi Dannielle A. Brazier Shane D. Campbell

Parthenium hysterophorus L. is highly invasive across many countries, impacting agriculture, human and animal health and the environment. Despite a widespread invasive range, the persistence of P. hysterophorus seeds (achenes) in the soil profile has been identified as a gap in current knowledge. A long-term seed persistence field trial was under-taken, where packets of P. hysterophorus seeds were buried, retrieved periodically over 10 years and the viability assessed. Time, burial depth and soil type (clay or loam) significantly influenced seed viability. Overall viability was <5% after 5 years, and <0.2% of viable seed was recovered after 7 to 10 years. The decline was faster in seeds at 0 cm (on the soil surface) and buried at 20 cm and slower in seeds buried at 2.5 cm and 10 cm. At most retrieval times viability was higher in seeds retrieved from clay soil plots than from loam soil plots. Pasture cover (present or excluded) did not significantly influence the viability of seeds. Data from controlled ageing laboratory experiments indicated persistent seeds and complemented the field trial data. Based on the weight of 1000 seeds, less than 20 mm of rainfall could incorporate 50% of seeds into three soil types. Parthenium hysterophorus develops a persistent seed bank when incorporated into the soil and infestations will require longer-term control even if seed input is prevented.

Seeds doi: 10.3390/seeds5040039

Authors: Ana Paula Braido Pinheiro Vinicius de Souza Oliveira João Vitor Garcia Silva Adriele dos Santos Jardim André Luiz Ribeiro Azeredo Verônica D’Addazio Fábio Ribeiro Pires Janyne Soares Braga Pires Edilson Romais Schmildt Luis Fernando Tavares de Menezes Adriano Alves Fernandes Sara Dousseau-Arantes

The success in the recovery of degraded areas is dependent on the acquisition of quality seedlings coupled. However, nursery activity is complex and involves significant operational and input costs. The present work had as its objective to evaluate the effects of the substrate and the chemical fertilization on the production and quality of Canavalia rosea (Sw.) DC. seedlings and their respective production costs. Ten treatments were tested, consisting of two substrates with sand: clay:coconut fiber (2:1:1 and 1:2:1) and five fertilizations (without fertilizer, with simple superphosphate, simple superphosphate + micronutrients, with NPK 04-14-08, and NPK 04-14-08 + micronutrients). The emergence speed index, emergence percentage, seedling height, stem diameter, leaf number, leaf area, shoot, root, and total dry mass, and Dickson quality index were evaluated. The 1:2:1 substrate and the fertilization levels with simple superphosphate, with simple superphosphate + micronutrients, with NPK 04-14-08, and with NPK 04-14-08 + micronutrients were higher than the control, with values 17% higher for seedling height, 5.4% for the number of leaves, and 18.9% for leaf area. Aiming at the low cost and the quality of the seedlings, it is recommended to use the substrate in the proportion 1:2:1, and fertilization with simple superphosphate.

Seeds doi: 10.3390/seeds5040038

Authors: Gonzalo Joaquín Arata Mailén Riveira-Rubin Diego Batlla María Verónica Rodríguez

In dormant sunflower achenes, several structures—the pericarp, seed coat and embryo—contribute to the repression of germination. Achene dormancy varies widely among cultivated sunflower genotypes, and understanding its transmission to hybrid progeny is important both for hybrid seed production and for clarifying the role of these structures. This study examined the inheritance of dormancy in the F1 progeny, with particular emphasis on thermo-inhibition (inhibition of germination at warm temperatures). Reciprocal crosses were performed using three oilseed inbred lines with contrasting dormancy phenotypes. Germination of achenes, seeds, and embryos was tested at 10 and 30 °C at harvest and during postharvest, together with hormonal responses (abscisic acid, ethylene and gibberellins) and measurements of endogenous ABA levels. Results show that maternally inherited, pericarp-imposed thermo-inhibition depends on the dormancy level of the hybrid embryo, which follows a zygotic pattern with incomplete dominance. While embryo sensitivity to ABA related positively with thermo-inhibition, surprisingly, embryonic ABA content was inversely related to dormancy level across genotypes. These findings provide new insight into physiological control of achene dormancy in sunflower and contribute to improved breeding strategies for high-quality hybrid seed.

Seeds doi: 10.3390/seeds5040037

Authors: Ahmed Othmani Karim Kadri Salem Marzougui Amel Sellemi Stefaan P. O. Werbrouck

This study reports the first documented occurrence of double-seeded fruits (DSF) in date palm (Phoenix dactylifera L.), a phenomenon distinct from previously described polyembryony. DSF were observed only in the cultivars ‘Kentichi’ and ‘Deglet nour’, where they occurred at very low frequencies (2.8 × 10−3% and 1.6 × 10−4%, respectively). Morphological observations indicate that DSF arise through partial or complete fusion of two carpels, resulting in syncarpic fruits that are significantly heavier and wider than single-seeded fruits, while fruit length remains unchanged. Germination rates were high and similar in both groups, but seeds from DSF germinated 8 days earlier than those from single-seeded fruits. In contrast, seedlings derived from DSF showed slower early growth. These findings identify DSF as a rare, genotype-dependent developmental variant in date palm and suggest that syncarpy influences fruit morphology, seed allocation, and germination behaviour.

Seeds doi: 10.3390/seeds5040036

Authors: Chitra Lekhwar Yogesh Kumar Murlidhar Meghwal Rajat Suhag

This study investigates the influence of normal and cryogenic grinding on the antioxidant properties and bioactive compound profiles of fennel seed (Foeniculum vulgare) powder at varying particle sizes. Methanolic and ethanolic extracts were evaluated for DPPH radical scavenging activity (AAO), total phenolic content (TPC), and total flavonoid content (TFC) using conditions optimised through Response Surface Methodology (RSM) employing an I-optimal quartic design. Statistical analysis confirmed that grinding type and solvent type were the dominant factors influencing all three responses, with their interaction significantly governing flavonoid content recovery. The optimal extraction conditions were identified as cryogenic grinding, methanol as solvent, and a particle size of 200 µm, with an overall desirability of 0.887. Validated experimental values under these conditions were AAO = 87.11%, TPC = 11.31 mg GAE/g, and TFC = 14.18 mg QE/g, with prediction errors within ±5% of model-predicted values confirming the robustness of the developed models. HRLC-MS analysis confirmed that cryogenic grinding preserves a broader and more concentrated phytochemical profile compared to normal grinding. These findings demonstrate that cryogenic grinding combined with methanol extraction at fine particle sizes significantly enhances the yield of antioxidant-rich phytochemicals from fennel seeds, supporting their potential application in functional food and nutraceutical development.

Seeds doi: 10.3390/seeds5040034

Authors: Alan Mario Zuffo Francisco Charles dos Santos Silva Adriana Araujo Diniz Augusto Matias de Oliveira Fábio Steiner Jorge González Aguilera Luis Morales-Aranibar João Flávio Floriano Borges Gomides Charline Zaratin Alves

Abiotic stresses, such as drought, salinity, and aluminum toxicity (Al3+), affect the growth and initial establishment of wheat plants, limiting crop yield in restrictive growing environments. Therefore, the early selection of tolerant genotypes adapted to multiple production environments is essential to optimize wheat production. A laboratory experiment was conducted to identify and recommend wheat cultivars that simultaneously combine adaptability and stability for initial morphological responses when subjected to stressful environmental conditions. Plants from 12 wheat cultivars were grown under non-stressful (control) and stressful conditions (drought, salinity and Al3+ stress), using a 4 × 12 factorial arrangement with four replicates. On the 28th day, the emergence rate, length, dry matter and vigor of the plants were measured. Abiotic stresses limit the initial growth and vigor of wheat plants, with drought causing the greatest limitation for plant growth and biomass accumulation, while salinity had the greatest impact on plant vigor indices. Aluminum toxicity limits root development and biomass allocation. Principal component analysis explained 67.76% of the total variability and distinguished the plant growing environments. The multi-trait index proved effective in cultivar selection, highlighting the cv. ORS Feroz due to its proximity to the ideotype and adaptability to multiple abiotic stresses.

Seeds doi: 10.3390/seeds5040035

Authors: Dennis Morgia Gilbero Mitch Tinambunan Bengil Mhar Ortiz Loquez Joan Sabejon Gilbero

Seed enhancement technologies have emerged as promising approaches to improve seedling growth and nursery performance of forest tree species. This study evaluated the effects of combining seed priming and seed coating technologies with beneficial microbial inoculants on the seedling quality of Falcataria falcata (L.) Greuter & R.Rankin. Fourteen treatments, including hydropriming (HP), gibberellic acid (GA3), Rhizobium sp., Trichoderma sp., endomycorrhiza, polymer coating, nutrients, fungicide, and insecticide, were assessed under nursery conditions. Seedling quality was determined using the number of roots, number of nodules, root-to-shoot ratio, vigor index I, and vigor index II. Significant differences among treatments were observed for all measured parameters (p < 0.001). The treatment HP + GA3 + Rhizobium sp. + polymer coat + fungicide (T13) produced the highest number of roots (31.76 roots seedling−1), indicating enhanced root development. Meanwhile, HP + endomycorrhiza (T4) resulted in the highest number of nodules (5.49 nodules seedling−1), root-to-shoot ratio (0.593), and vigor index I (2055.57), reflecting improved biomass allocation and overall seedling quality. Principal component analysis explained 71.9% of the total variation and revealed distinct associations between treatments and growth attributes. Treatments containing Rhizobium sp. were primarily associated with root proliferation and seedling vigor, whereas endomycorrhizal treatments were linked to nodulation and balanced biomass development. The results demonstrate that integrating microbial inoculants with seed priming and coating technologies can significantly enhance seedling quality, even when germination responses are similar among treatments. These findings highlight the potential of biologically enhanced seeds as a sustainable strategy for producing vigorous planting materials suitable for plantation forestry, reforestation, and landscape restoration programs.

Seeds doi: 10.3390/seeds5030033

Authors: Dimosthenis Chachalis Nikolina Vidali Aggeliki Petraki Dimitrios Vlotsos Athina Motsenigou

Direct-seeded industrial tomato (Solanum lycopersicum L.) systems are highly vulnerable to early-season interference, yet the role of seed vigor as a competitive determinant remains under-quantified. This study evaluated the performance of high-vigor (HV; 91% germination) and accelerated-aged low-vigor (LV; 60% germination) tomato seeds against two weeds: green foxtail (Setaria viridis) and jimsonweed (Datura stramonium). While mean emergence timing was statistically comparable between HV and LV cohorts (6.0 vs. 7.2 days), LV seedlings entered the post-emergence phase with a numerical deficit in initial seedling dry weight (7.1 mg vs. 8.5 mg for HV; difference not statistically significant), suggesting a potential early competitive disadvantage. In replacement series experiments, HV tomatoes maintained stable leaf and root biomass within the 0.76–1.24 relative yield (RY) confidence interval when competing with jimsonweed. In contrast, LV plants were significantly suppressed at low weed proportions (25%), where root RY dipped below the 0.76 threshold. Against the aggressive below-ground strategy of S. viridis (which produced ~1200 mg of root mass by 40 DAE), LV tomato root RY collapsed to 0.10–0.15, whereas HV plants maintained significantly higher niche occupancy. Physical separation of above- and below-ground competition confirmed that HV seeds provide a “physiological buffer”; specifically, in below-ground treatments, HV plants achieved a root mass of 0.25 g/plant compared to only 0.15 g/plant for LV plants. These results identify seed vigor as a primary driver of the “priority effect” and suggest that high-vigor lots are essential for Integrated Weed Management (IWM) strategies to mitigate early-season resource pre-emption. These findings suggest that seed vigor assessment should be integrated into seed quality standards for direct-seeded tomato systems as a component of Integrated Weed Management. Future field-based studies are needed to validate these greenhouse findings under variable agronomic conditions.

Seeds doi: 10.3390/seeds5030032

Authors: Teodora Tomova Atanas Senin Erol Eshrefov Desislav Tomov Iva Slavova Mariana Argirova

Ginkgo biloba seeds are a rich source of flavonoids and the unique terpene lactones—ginkgolides and bilobalide, known for their neuroprotective and cognitive-improving effects. However, unlike the widely used leaves, the seeds contain substantial levels of ginkgolic acid and ginkgotoxin (4′-O-methylpyridoxine), an antivitamin B6 compound. At high concentrations, ginkgotoxin exhibits neurotoxicity, potentially inducing seizures, respiratory distress, and loss of consciousness, thus limiting the safe application of Ginkgo seed-derived products. This study aimed to develop a simple yet effective extraction protocol that reduces ginkgotoxin levels in Ginkgo biloba seed extracts while preserving their beneficial phytochemicals. A multistep liquid–liquid extraction approach employing sequential polar and non-polar solvents was implemented. Following each extraction stage, fractions were analyzed using ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC–MS). The concentrations of flavonoids, ginkgolides, bilobalide, ginkgolic acid, and ginkgotoxin were quantified to evaluate detoxification efficiency and phytochemical retention. Compared with conventional single-step extraction using 70% methanol, this multistep protocol markedly reduced ginkgotoxin and ginkgolic acid to near-undetectable levels, while preserving detectable concentrations of major flavonoids and terpene trilactones. The findings demonstrate that multistep extraction represents a promising and practical strategy for minimizing ginkgotoxin in Ginkgo biloba seed extracts without compromising their beneficial phytochemical composition. This approach provides a sound basis for developing safer, functionally active Ginkgo-based products.

Seeds doi: 10.3390/seeds5030031

Authors: Javier Giovanni Álvarez-Herrera Marilcen Jaime-Guerrero Dilson Sebastián Torres-Piña

Pea cultivation has witnessed significant growth in international trade in recent years, leading to increased export volumes worldwide. However, seed germination and early seedling growth often exhibit low uniformity, resulting in heterogeneous seedling sizes, which limit agronomic management and affect overall performance. As a result, this study aimed to assess the effects of gibberellin (GA) doses on the germination of the ‘Santa Isabel’ pea variety, one of Colombia’s most commonly cultivated varieties. A completely randomized design was employed with five treatments (0, 50, 100, 150, or 200 mg L−1). The application of 200 mg L−1 GA significantly enhanced germination percentage, germination potential, and germination speed index by 66.4%, 64.9%, and 71.5%, respectively, compared to the control. Furthermore, it increased the vigor index. The GA application reduced the mean germination time to 6.48 days, while the control exhibited 8.98 days. GA treatment increased seedling height to 5.3 cm, compared with 3.0 cm in the control. The variation coefficient in germinated seedling height increased as germination progressed and stabilized towards the end. Although GA did not affect the total fresh mass of the seedling, it did influence the proportion of mass allocated to each organ. Notably, there was a decrease in the amount of photoassimilates transferred from the seed to the leaves and stipules, accompanied by an increase in dry and fresh mass in the stems. The control treatment exhibited the highest fresh and dry leaf mass values compared with the GA-treated treatments.

Seeds doi: 10.3390/seeds5030030

Authors: Anagha Wankhade Zhiting Xu Ashlynn Clark David Britt

Seed-associated endophytes become active during germination, playing important roles as early colonizers of plant tissues and contributing to plant health while residing in a protective niche. In this study, we characterized a wheat-derived bacterial isolate, JunSE1L, to determine its functional traits and ecological role in the plant microbiome. The isolate was identified as Bacillus atrophaeus based on 16S rRNA analysis. JunSE1L exhibited nutrient-dependent plasticity in colony architecture, forming robust hydrophobic biofilms and pellicles under rich nutrient availability while swarming and forming thin, often dendritic colonies under defined nutrition. JunSE1L produced highly surface-active compounds that lowered the surface tension of water to 30 mN/m and released potent proteolytic and hemolytic compounds, thus equipping JunSE1L for antagonistic interactions, as examined against several fungal pathogens. JunSE1L inhibited Fusarium proliferatum and Mucor hiemalis in live-cell assays, while cell-free supernatant selectively inhibited M. hiemalis. JunSE1L was recovered from multiple plant compartments, including rhizosphere, rhizoplane, and aerial tissues, and was observed to emerge from cut plant tissues, supporting seed-endophyte mobilization upon germination to colonize distal tissues. Seed surface inoculation experiments with JunSE1L showed limited attachment at low cell densities and reduced seedling vigor at higher inoculum levels, indicating that inoculum density and native microbiome interactions influence seedling performance.

Seeds doi: 10.3390/seeds5030029

Authors: Saoirse O’Neill Field Antonia Alessandra Lemos Dos Santos Elena Grosu Eoin O’Connor Brian O’Connor Colin T. Kelleher Dheeraj Singh Rathore

The long-term storage of seeds is important for conserving native species, but its effectiveness depends on maintaining seed quality. This study assessed the impact of storage duration on seed quality in Alnus glutinosa (alder) and Betula pubescens (downy birch). Seed quality was evaluated using thousand-seed weight (TSW), moisture content (MC), tetrazolium (TZ) viability, and germination tests. Results from stored seed lots were compared with those from recently collected seeds. Moisture content, TZ viability, and germination were significantly affected by storage duration, although sensitivity analyses indicated that storage conditions, particularly lower temperature and airtight storage, may have contributed to improved seed viability in specific seed lots. A relationship between TZ viability and germination was observed, although this was influenced by zero values. Cold stratification improved germination in downy birch but did not compensate for reduced viability in older seed lots. These findings highlight the importance of storage conditions and species-specific pre-treatments and support the use of TZ testing as a rapid indicator of seed viability when used alongside germination testing. The results provide practical guidance for managing seed resources and maintaining reliable forest reproductive material supply.

Seeds doi: 10.3390/seeds5030028

Authors: Lilibeth Andujo-Ponce Celia Chavez-Mendoza Alexandro Guevara-Aguilar Esteban Sánchez Alma Delia Alarcón-Rojo Elia Cruz-Crespo Martin Juárez-Morales

Achiote has been used since pre-Hispanic times, but today it is undervalued in Mexico and is threatened with disappearance in some regions. Therefore, it is important to focus research on this crop to enhance its value and prevent the loss of this ancestral germplasm, which is an essential part of Mexican, Latin American, and Caribbean cuisine and a source of income for small rural and indigenous producers. Furthermore, it has the potential to increase its commercial value as a natural colorant compared to synthetic ones. However, despite its properties, there is currently no information available on the nutritional characteristics of the seed produced in various regions of Mexico. The aim of this study was to determine the physicochemical characteristics, nutritional value, and antioxidant compound content of achiote seeds from Nuevo Huixtán Margaritas Chiapas (Margaritas), San Pedro Tapanatepec Oaxaca (Oaxaca), and Nuevo Jericó Palenque, Chiapas (Palenque), Mexico. The effect of the region on morphological and physicochemical characteristics, nutritional quality, and nutraceutical properties of the seed was evaluated. A statistically significant difference (p ≤ 0.05) was obtained between regions for all variables studied except for the brightness (L*) of ground seeds and the content of total phenols, C, N, and S. Margaritas seeds were the heaviest and longest, while those from Oaxaca were the smallest and presented the highest values for all whole seed color parameters, in addition to having the best nutritional quality. The antioxidant capacity (DPPH) obtained was 70.01 to 76.96% inhibition, with the maximum values found in seeds from Oaxaca and Palenque. In conclusion, Mexican achiote seeds exhibit notable nutritional and nutraceutical properties, which vary depending on the region of production, highlighting the influence of geographic origin on their composition.

Seeds doi: 10.3390/seeds5030027

Authors: Georgios Varsamis Eleftherios Karapatzak Anna Vasiou Theodora Merou

Urbanization reduces biodiversity and affects plant–insect interactions, creating a need for more functional green spaces. Urban meadows with native species are a promising option, but their use is still limited due to a variety of reasons concerning the utilization framework of suitable plant species. The present study aimed to develop seed germination protocols for 26 native Mediterranean herbaceous species originating from northeastern Greece selected to support the establishment of species-rich and self-sustaining urban meadows. To the above end, seed germination experiments were conducted ex situ under controlled environment conditions using seeds collected from the wild for each species. Seed viability was assessed using the tetrazolium (TTZ) test to determine the maximum germination potential in each case. Freshly collected seeds were stored under ambient conditions for approximately 3 months (after-ripening) prior to germination testing, which was followed by cold stratification as a pretreatment for dormancy release. The results showed high embryo viability in all species and indicated that most taxa exhibited either no dormancy or relatively shallow physiological dormancy. Germination tests revealed that 14 of the 26 species presented high germination percentages in the control treatment, which suggests that after-ripening contributed to dormancy release in a significant portion of the seed lot. However, it remains unclear whether freshly collected seeds require an initial after-ripening period before responding to cold stratification. Furthermore, cold stratification significantly enhanced germination in 12 species confirming its effectiveness as a simple and practical method for dormancy release. In addition to the seed germination results, the selected species present a wide range of functional and esthetic characteristics, including variation in plant height, flowering phenology and flower and leaf color. These traits are important for both ecological performance and visual quality in urban environments. The combination of extended flowering periods and color diversity suggests the potential for continuous floral resource availability, which can support diverse pollinator communities and, indirectly, urban fauna such as insectivorous birds. The results indicate that the studied species are suitable for biodiversity-oriented urban plantings. Their relatively shallow dormancy and ease of propagation, coupled with their functional and aesthetic traits, support their use in the development of resilient and self-sustaining urban meadows.

Seeds doi: 10.3390/seeds5030026

Authors: Jackelin Ruiz-Vidal Georgina Vargas-Simón Guillermo Angeles José Ángel Gaspar-Génico Lilia María Gama Campillo Nelly del Carmen Jiménez-Pérez Pablo Martínez-Zurimendi Jesús Ascencio-Rivera

Ormosia macrocalyx grows in tropical forests and is endangered in Mexico. The species has ecological and economic importance. To evaluate the relationship between fruit length and seed number, Pearson correlation and principal component analysis were used. A linear mixed-effects model was also applied. Pearson correlation, principal components analysis (PCA) and a Linear Mixed-Effects Model (LMM) were performed on an exploratory basis. In addition, seed coat and cotyledon anatomy were examined, and histochemical tests for secondary metabolites were carried out. Two high correlations and two components were obtained from the PCA, and the LMM showed that fruit length influenced the number of seeds per fruit. In the seed coats, differentiated layers of macrosclereids and osteosclereids were identified, where the hilar region presented macrosclereids and a pyriform bar of tracheids, the reserved cotyledons showed double-walled cells and simple plasmodesmata, the histochemical analyses demonstrated the presence of cellulose, condensed tannins, lipids, alkaloids, and proteins, and no starch was present. This study provides the first description of seed coat and cotyledon anatomy in O. macrocalyx, as well as the first report of secondary metabolites in storage cotyledons. These results could be useful for further studies of this species.

Seeds doi: 10.3390/seeds5030025

Authors: Md. Anwar Hosen Jony Bejoy Chandra Sarkar Sinthia Ahmed Upama Sinthia Afsana Kheya Md. Shafiqul Islam Farhana Zaman Ahmed Khairul Hasan

Salinity stress is a major constraint affecting rice establishment and productivity in many coastal and salt-affected regions of the world, as well as in Bangladesh. Seed priming has emerged as an effective technique to enhance seed germination, seedling vigor and growth, and stress tolerance. To address this challenge, the present study investigated the potential of four different seed-priming agents (non-, hydro-(H2O), osmo-(Polyethylene glycol, 30%), nano-(Zinc EDTA (12%), and 170 ppm) applied to two rice varieties (Binadhan-10 and BINA dhan25) under four levels of salinity stress (0, 5, 8, and 11 dS m−1), with the aim of enhancing germination, improving the seedling vigor index, and promoting early growth performance in a completely randomized design with four replications. Nano-priming with Zinc EDTA (12%, at 170 ppm) involves soaking seeds in a solution containing this concentration of zinc chelate, which can improve seedling vigor and stress resilience, especially under challenging conditions like salinity. The results indicated that salinity significantly reduced germination and seedling growth, whereas seed priming improved seed performance under stress conditions. Among the treatments, nano-priming showed the most pronounced improvement in germination and seedling vigor. Binadhan-10 exhibited a greater tolerance to salinity compared with BINA dhan25. Multivariate analyses, including principal component analysis, correlation analysis, and heatmap, revealed strong positive relationships among germination, vigor index, and seedling biomass traits. The findings demonstrate that seed priming, particularly nano-priming, can effectively enhance rice seed germination, the vigor index, and different seedling traits under saline conditions, providing a promising strategy for improving rice production in salt-affected areas in Bangladesh.

Seeds doi: 10.3390/seeds5030024

Authors: Konstantinos Krommydas Athanasios Mavromatis Fotios Bletsos Demetrios Roupakias

The utilization of cytoplasmic male sterility (CMS) is essential in hybrid seed production; however, its operational value in eggplant is insufficiently documented under practical conditions. This study compared CMS-based and conventional (non-CMS) hybridization systems with respect to flower production, flower functionality, and hybrid seed production efficiency, quantified as seed and viable seedling output per unit time, in three eggplant cultivars (‘Emi’, ‘Langada’, and ‘Tsakoniki’) in intra- and interspecific crosses. CMS did not affect total flower production or inflorescence architecture, which were primarily genotype-dependent. However, it altered flower opening, resulting in genotype- and position-dependent proportions of semi-opened and closed flowers at anthesis. Despite this effect, sufficient flowers suitable for hybridization remained available across all genotypes. CMS substantially simplified the hybridization process by eliminating emasculation, reducing flower manipulation time by approximately 55%, and increasing crossing rate by nearly twofold. Importantly, CMS did not negatively affect female fertility, as indicated by comparable percentages of successful crosses and high seed germination rates across cytoplasmic backgrounds. Seed production per fruit was moderately but significantly increased in intraspecific crosses, while it remained comparable between CMS and non-CMS systems for the interspecific crosses. As a result, CMS significantly increased hybrid seed output and effective seedling production per unit time in intraspecific crosses, while similar trends were observed in interspecific crosses, with gains ranging from 86% to 184% depending on genotype and pollen parent. Overall, this study demonstrates, from an operational perspective, that CMS enhances the efficiency of eggplant hybrid seed production by reducing labor requirements and increasing output per unit time without compromising reproductive performance. These findings highlight the practical value of CMS as a tool for improving hybrid seed production systems, including applications in both commercial hybrid development and rootstock breeding.

Seeds doi: 10.3390/seeds5030023

Authors: Aroua Kouadri Hafida Trabelsi Abdelmadjid Chehma Lacramioara Oprica Marius-Nicusor Grigore

Plant–animal relationships, such as endozoochory, are crucial for ecosystem dynamics as they improve seed dispersal as well as enhance plant reproductive success. This study investigated the ecological role of camels in seed dispersal and in breaking physical dormancy of three wild perennial Fabaceae species (Retama raetam, Vachellia tortilis subsp. raddiana, and Genista saharae) dominant in Algerian Saharan rangelands. Feeding trials were conducted with four adult female camels (Camelus dromedarius). Each animal received 1500 manually extracted seeds per species in three doses mixed with the regular ration, and feces were collected for 24 days to recover seeds. Untreated control seeds were kept under identical environmental conditions. The results showed that camels contribute to seed dispersal and influence germination differently among species. Seeds of Retama raetam were rapidly excreted, favoring local dispersal and increasing germination from 8% to 32%. Genista saharae seeds showed early excretion and longer retention, promoting wider dispersal and increasing germination from 16% to 52%. Vachellia tortilis subsp. raddiana showed an intermediate pattern, with germination rising from 39% to 70%. Gut passage affected seed size in Genista saharae and Vachellia tortilis subsp. raddiana, while no change occurred in Retama raetam. These findings highlight the camel’s ecological role as a biological vector improving seed viability and supporting regeneration.

Seeds doi: 10.3390/seeds5020022

Authors: Audrone Ispiryan Elvyra Jariene Alina Butu

Rose hip processing generates seed-rich by-products that remain underexplored beyond oil extraction, despite their potential as a source of phenolic compounds and antioxidant activity. This study investigates the effect of bioprocessing (short-term fermentation) on the phenolic composition and antioxidant activity of rose hip (Rosa spp.) seed by-products, with relevance to cosmetic-oriented applications related to oxidative stress modulation. Rose hip seeds were obtained after juice production and subjected to short-term fermentation (14 days at 21 °C) using Saccharomyces cerevisiae, followed by mechanical separation and drying. Non-fermented and bioprocessed seeds were analyzed for individual phenolic compounds and antioxidant activity (DPPH, ABTS, FRAP), and correlation and multivariate analyses were conducted. Bioprocessing reduced total identified phenolics from 15.79 to 10.72 mg/g DW (≈32%), primarily due to a decrease in epigallocatechin (10.89 to 6.50 mg/g DW). In parallel, the relative contribution of phenolic acids increased, including gallic acid (0.50 to 0.60 mg/g DW) and salicylic acid (0.98 to 1.20 mg/g DW), indicating a selective compositional redistribution accompanied by partial degradation. Antioxidant activity decreased after bioprocessing (DPPH ~340 to ~250 µmol TE/g DW) but remained substantial. Correlation analysis identified epigallocatechin as the main contributor to antioxidant capacity. These findings show that rose hip seeds behave as a process-sensitive phenolic matrix in which bioprocessing alters the balance of individual compounds without complete loss of antioxidant activity. The results indicate that seed-derived by-products retain functional potential for further valorization in cosmetic-oriented applications.

Seeds doi: 10.3390/seeds5020021

Authors: Anteneh Agezew Melash Gashaw Gismu Chakilu Amare Assefa Bogale Éva Babett Ábrahám

Durum wheat (Triticum turgidum L. var. durum) plays a central role in global food and nutritional security, yet its grain mineral and protein quality remain highly variable across varieties and environments. This study aimed to evaluate the combined effects of varieties, nitrogen (N) fertilization, foliar zinc (Zn) and sulfur (S) application, and seasonal variation on grain macro- and micronutrients (Ca, Mg, K, Zn) and protein content, adopting a system-level approach that integrates varieties, nutrient management, and seasonal variability. We hypothesized that aligning variety selection with precision nutrient management would improve grain nutritional quality while enhancing resilience to environmental variation. Significant differences among varieties (p < 0.05) were observed, with the spring-sown variety Durablank exhibiting the highest grain Zn concentration (34.70 mg kg−1) compared with MV Pelsodur (23.0 mg kg−1) and GK Julidur (23.8 mg kg−1), representing a 50.87% varietal difference. Grain Ca, Mg, and K varied widely across varieties and seasons, with drought in 2022 increasing grain Zn, while the wetter 2023 season enhanced Mg and K accumulation. A clear yield–protein trade-off was found, where high-yielding MV Pelsodur maintained 14.3% protein while moderate-yielding Durablank reached 16.8%. Foliar Zn and S applications significantly increased grain Zn, particularly in Zn-efficient varieties. Nitrogen fertilization at 100 kg ha−1 improved macronutrient accumulation by 12–20% compared with 60 kg N ha−1. Significant varieties × N × season interactions (p < 0.01) highlight the need for adjusted varieties -specific nutrient management strategies. Overall, the findings demonstrate that integrating genetic selection with optimized fertilization and season-responsive management practices may contribute to improving grain nutritional quality of durum wheat.

Seeds doi: 10.3390/seeds5020020

Authors: Paula Garbacea Emanuel Stoica Alin-Madalin Alexandru Georgeta Mihai Katri Himanen Heino Konrad

The collection of high-quality seeds to produce forest seedlings is closely linked with the time of harvesting. Climate warming is already having visible effects in all life stages of forest tree species, including the timing of seed maturation. The purpose of this study was to update the knowledge on seed collection timing and to identify the indicators of physiological maturity for three key Eastern European tree species—silver fir (Abies alba), European larch (Larix decidua), and small-leaved lime (Tilia cordata). Seeds and cones were collected from Romanian clonal seed orchards and evaluated at several stages of seed maturation using germination tests for European larch and tetrazolium viability tests for silver fir and small-leaved lime. The results revealed species-specific differences in seed maturation timing: in silver fir seed viability increased slightly from late August to early September, in European larch germination remained low (≈20%) regardless of harvest time, while small-leaved lime viability declined significantly after late August. These findings suggest that the harvest period observed during the study years occurred earlier than the traditionally recommended intervals and could be linked to recent warming trends. This study highlights the relevance of re-evaluating seed collection schedules under changing climatic conditions, while further multi-year studies are required to confirm these patterns and refine practical recommendations.

Seeds doi: 10.3390/seeds5020019

Authors: Joyce Naiara da Silva Guilherme Vinícius Gonçalves de Pádua Caroline Marques Rodrigues Eduardo Luã Fernandes da Silva Aline das Graças Souza Edna Ursulino Alves

Viviparity is rarely reported in species with seed physical dormancy. This is because physical dormancy, common in tropical trees, results from the impermeability of the seed tegument to water and regulates the timing of germination. Records of germination within the fruit in such species suggest greater functional plasticity than traditionally assumed. This study reports and quantifies viviparity in Hymenaea martiana seeds over four consecutive years (2019–2022) and assessed seed dormancy status through vigor tests. Sixty fruits were collected annually, and viviparity was estimated as the proportion of fruits containing at least one seed with radicle protrusion. Non-viviparous seeds were subjected to emergence tests with and without mechanical scarification, and cumulative emergence, percentages of dead and water-impermeable seeds, and seed water content were evaluated. Viviparity occurred consistently, affecting approximately 10–15% of the fruits, with no significant differences between years, indicating that the phenomenon was not associated with extreme environmental events. However, early germination did not result in seedling establishment because the emerging roots dehydrated while still inside the fruit. Mechanical scarification significantly increased emergence, confirming the presence of seed physical dormancy. These results show that, although viviparity was observed recurrently in the studied population of H. martiana, seed physical dormancy remains the main mechanism controlling germination in this species.

Seeds doi: 10.3390/seeds5020018

Authors: Rafael Ruiz-Hernández Arturo Pérez-Vázquez Fredy Morales-Trejo Gustavo López-Romero José Roberto Bautista-Aguilar Mario Alejandro Hernández-Chontal Emmanuel de Jesús Ramírez-Rivera Oliver Salas-Valdez Adán Cabal-Prieto

The objective of this research was to apply morphometric and chemometric techniques to analyze the influence of climate and soil type on the morphological, proximate, and fatty acid fingerprints of moringa (Moringa oleifera Lam.) seeds cultivated in different regions of Mexico. Seeds were collected from the states of Chiapas, Michoacán, Nuevo León, Oaxaca, Veracruz, and Yucatán. The morphological traits of the seeds were evaluated, while the proximate composition and fatty acid profiles of the seed flours were analyzed using gas chromatography–mass spectrometry (GC–MS). Data were assessed through analysis of variance (ANOVA) and linear discriminant analysis to develop their fingerprint profiles. The results showed that the morphological variables that constituted the climate-based morphological fingerprint were seed length, width, seed weight, and kernel weight, whereas for the soil type-based fingerprint, only seed length was significant. Regarding the proximate chemical composition, all variables (fat, ash, moisture, and protein), except fiber content, were influenced by both climate and soil type, forming the proximate chemical fingerprint. The fatty acid fingerprint consisted of 21 compounds, with oleic, behenic, stearic, palmitic, and arachidic acids present in the highest concentrations. The fingerprints obtained from the different determinations were confirmed through cross-validation values exceeding 50%, according to the linear discriminant analysis validation technique. The fatty acid and proximate composition determinations showed the highest classification values (83–100%) and contributed most significantly to ensuring the fingerprinting of moringa seeds cultivated in Mexico.

Seeds doi: 10.3390/seeds5020017

Authors: Sota Inomata Yudai Kudo Kohei Koyama

Seed dispersal by humans plays an important role in determining vegetation structure. The seeds of Asian plantain (Plantago asiatica L.) form adhesive mucilage upon hydration, facilitating their attachment to shoes and subsequent dispersal via epizoochory. We investigated the efficacy of this mechanism under various urban environmental conditions. After trampling wild P. asiatica stands, the number of seeds attached to shoe soles was counted. The remaining seeds were then counted after walking at designated distances (1, 2, 5, 10, 20, 50, 100, 200, 500, and 1000 m). The following results were obtained: (1) The retention rate after walking 1000 m varied by shoe type (slip-on (kakkusu) work shoes, 15.4%; leather shoes, 3.4%; rubber boots, 2.7%; running shoes, 13.5%; and sandals, 12.4%). (2) Within the first 50 m of walking, on average more than half of the attached seeds fell off under all investigated conditions. Significantly fewer seeds remained after walking 50 m on asphalt (30.9% of the initial seeds) than on grass (48.2%), whereas after walking 1000 m, similar proportions (15.4% on asphalt and 15.7% on grass) remained on the work shoes. These results indicate that human-mediated short- and long-distance dispersal of mucilaginous seeds of this species is effective in diverse urban environments.

Seeds doi: 10.3390/seeds5020016

Authors: Manisha Poudel Chintamani Panjiyar Hari Prasad Pandey Vijay Kumar Yadav Tek Maraseni

Moringa oleifera (Lam.) is a multipurpose agroforestry tree cultivated worldwide for its nutritional, medicinal, and economic value, and it is increasingly grown commercially in subtropical regions, including Nepal. While vegetative propagation is feasible, large-scale production relies predominantly on seeds, making efficient germination critical for seedling establishment, uniform growth, sustainable production, and preservation of genetic diversity. Seed pre-treatments are widely recognized as a simple and effective approach to enhance germination, early seedling vigor, and nursery performance. This study evaluated the effects of seven pre-sowing treatments under controlled nursery conditions to determine the most effective method for improving Moringa oleifera seedling production. A total of 2100 seeds were used, with 100 seeds per treatment and three replicates, arranged in a Completely Randomized Design (CRD). Treatments included control (no pretreatment), normal water soaking (12 h and 24 h), alternating wetting (water) and drying cycles (12 h each), hot water soaking (60 °C for 5 min), cow urine soaking (1:2 of urine to water proportions for 12 h), and hydrochloric acid soaking (35% for 20 min). All pre-treatments were conducted at room temperature, and the seeds were subsequently sown in controlled nursery conditions. Seed germination was monitored twice daily for 30 days, and data were analyzed using one-way ANOVA and Tukey’s HSD test to identify significant differences in germination performances. Results demonstrated that alternating wetting and drying produced the highest germination percentage (89%), shortest mean germination time (8.44 days), and strongest seedling vigor, outperforming all other treatments. Conversely, cow urine and acid treatments completely inhibited germination. This study recommends alternating wetting and drying as a simple, low-cost, and chemical-free pre-treatment to optimize Moringa oleifera seedling production in nurseries. These findings provide practical guidance for commercial and smallholder farmers, contributing to sustainable agroforestry, food security, and climate-resilient livelihoods in resource-limited habitats.

Seeds doi: 10.3390/seeds5010015

Authors: Collins Bugingo Thota Joseph Raju Swarnalatha Moparthi Jagmohan Singh Harish Madabahalli Shivanna Shaista Karim Andéole Niyongabo Turatsinze

Seed health testing is a cornerstone of global food security, yet traditional diagnostic workflows often struggle to balance speed, sensitivity, and regulatory confidence under low-prevalence and heterogeneous seed lot conditions. This review synthesizes recent advances in molecular diagnostics (PCR, qPCR, LAMP, and digital PCR), non-destructive imaging technologies (hyperspectral, X-ray, and thermal imaging), and data-driven analytical approaches for pathogen detection in seeds. Emphasis is placed on the practical integration of these tools within high-throughput, ISO/IEC 17025-compliant laboratory workflows, highlighting their respective strengths, limitations, and roles in risk-based decision-making. Comparative discussions address cost, sensitivity, turnaround time, and field deployability across diagnostic platforms, supported by crop- and pathogen-specific examples. Emerging approaches such as CRISPR-based biosensing, advanced sequencing, and imaging-assisted analytics are discussed in the context of validation, regulatory acceptance, and operational feasibility. By focusing on implementation rather than conceptual frameworks, this review provides a pragmatic reference for laboratories, regulators, and seed companies seeking to modernize seed health testing while safeguarding trade integrity and biosecurity.

Seeds doi: 10.3390/seeds5010014

Authors: Janyne Soares Braga Pires Francine Bonomo Crispim Silva Maria Eduarda da Silva Barbosa Geovana Ribeiro Cavilha Mateus Moura Coelho Samile Mardegan Otilia Josué Wan Der Maas Moreira Guilherme Roas Martins Marquito Fernando Gomes Hoste Ana Júlia Câmara Jeveaux-Machado Vinicius de Souza Oliveira Adriano Alves Fernandes Sara Dousseau-Arantes

Soil salinization is one of the main factors limiting agricultural productivity, negatively affecting seed germination and initial growth of maize (Zea mays L.). As a sustainable alternative, seaweed-based biostimulants, especially extracts of Ascophyllum nodosum, have stood out in mitigating abiotic stresses. This study aimed to evaluate the potential of A. nodosum extract in inducing tolerance to saline stress in maize seeds of the AL Bandeirante cultivar. To this end, three independent bioassays were conducted under controlled conditions: (i) evaluation of five doses of the extract (0; 0.5; 1.0; 1.5 and 2.0 mL L−1); (ii) effects of five osmotic potentials induced by NaCl (0, −0.2, −0.4, −0.6 and −0.8 MPa); and (iii) the interaction between the most efficient doses and salinity levels, comparing the extract to its mineral fraction. Seed germination, percentage of normal and abnormal seedlings, radicle and epicotyl length, and vigor index were measured. The results demonstrated that doses of 1.0 to 2.0 mL L−1 promoted greater bioactivity, with a 7.3% increase in root length compared to the control. Although increased salinity progressively reduced all variables, with severe effects at −0.6 and −0.8 MPa, the treatment with the extract showed superior performance to the mineral fraction, demonstrating a mitigating effect. It is concluded that A. nodosum extract is an effective strategy to attenuate the damage caused by salinity on seed germination and initial seedling growth in maize, especially under moderate stress.

Seeds doi: 10.3390/seeds5010013

Authors: Priscilla Yaa Asantewaa Akonnor Mavis Owusuaa Osei-Wusu Kwadwo Ofori

Solanum torvum is an important medicinal and culinary vegetable with poor seed propagation, characterized by low germination and limited seed longevity. This study examined the effects of fruit maturity stage, storage temperature, duration, and endogenous hormonal profiles on seed germination. Fruits were harvested at three physiological maturity stages: matured green, ripe yellow, and overripe brown. Extracted seeds were stored in ambient (24–26 °C) and cold (3–8 °C) conditions for 24 weeks, with subsequent germination testing with 3 replicates per treatment. Endogenous abscisic acid (ABA) and gibberellic acid (GA) were quantified using HPLC-DAD to assess their association with germination behaviour. Seeds from ripe yellow fruits achieved the highest germination (95%), with a mean germination time of 12 days and a mean germination rate of 8%, identifying this stage as the optimal maturity stage for harvest. While total germination percentage was enhanced by an after-ripening effect during the first 16 weeks of ambient storage, other vigour parameters, including mean germination time and rate and synchronization began to decline thereafter. ABA and GA concentrations displayed treatment-dependent variation across maturity stages and storage conditions, with hormonal trends showing complex associations with dormancy release rather than consistent main effects. These findings indicate that harvesting Solanum torvum fruits at the ripe yellow stage and storing seeds under ambient conditions for up to 16 weeks, under the conditions evaluated in this study, provides a practical balance between dormancy alleviation and seed vigour, thereby improving short-term propagation efficiency.

Seeds doi: 10.3390/seeds5010012

Authors: Luka Drenjančević Ivana Varga Goran Jukić Ivan Varnica Dario Iljkić

Even though wheat’s response to nitrogen (N) is well studied, practical optimization remains challenging because yield and seed quality often react inconsistently across seasons. For winter wheat, the simultaneous quantification of efficiency indicators that capture N losses and diminishing returns is important. This study evaluated nitrogen (N) fertilization in two growing seasons. This study aimed to adjust N fertilization strategy through different combinations of granular N timing and foliar applications to improve winter wheat yield and technological seed quality while maintaining high fertilization efficiency. Field experiments were conducted over two growing seasons (2021/2022 and 2022/2023) using seven fertilization treatments (Control, TSE_1, TSE_2, TSEH_1, TSEH_2, TSEH_3, and TSH, which correspond to growth stage T—tillering stage; SE—stem elongation phase; H—heading stage) in the range of 140.5 to 194.5 kg ha−1 N. Seed yield and seed quality traits (moisture, hectoliter weight, starch, protein, gluten, and sedimentation value) were measured, and treatment effects were evaluated with ANOVA, correlation, and regression analyses. In 2021/2022, no significant treatment effects were detected for yield or seed quality parameters, indicating that environmental variability dominated crop response. In contrast, in 2022/2023, seed yield, hectoliter weight, gluten content, and starch yield showed significant treatment effects (p ≤ 0.05–0.01), with fertilized variants generally outperforming the Control. Across both seasons, seed quality traits displayed strong internal structure: protein, gluten, and sedimentation were strongly positively correlated, while starch was strongly negatively correlated with these traits and the yield correlated positively with hectoliter weight but negatively with protein and gluten, highlighting a yield–quality trade-off. Importantly, partial factor productivity (PFP) and nitrogen use efficiency (NUE) showed the strongest treatment sensitivity, demonstrating their value for identifying efficient N strategies even when yield and quality responses were season-dependent. Regression analyses confirmed that seasonal conditions modulated nitrogen responsiveness, with NUE and starch yield showing much stronger relationships with nitrogen input in 2021/2022 and 2022/2023, respectively.

Seeds doi: 10.3390/seeds5010011

Authors: Bálint Gerbovits Edward Agyarko Omeralfaroug Ali András Szabó Sándor Keszthelyi

Damage caused by Brassicogethes aeneus primarily affects the reproductive organs of rapeseed, disrupting fertilization and often leading to premature pod opening. In addition to direct yield loss, it is hypothesized that injury to generative tissues may also alter seed nutrient composition, particularly the unsaturated fatty acid profile, which is a key determinant of rapeseed quality. To assess this indirect effect, field experiments were conducted in 2024–2025, and seed samples were collected after ripening. The study aimed to evaluate pest-induced variation in nutrient content under different insecticide treatments. Alongside an untreated control, two active substances were tested: a systemic insecticide (acetamiprid) and a combined formulation of acetamiprid and lambda-cyhalothrin. Our results demonstrated that the widely used active insecticides are not effective against B. aeneus adults. Checking the fatty acid (FA) profile, within saturated FAs, the proportion of palmitic acid (C16:0) was the highest in the control; the single and combined pesticide treatments were characterized by increasing LA (C18:2n6) and ALA (C18:3n3) levels, in which both FAs exhibited a linear pattern with the single and combined treatments. In MUFAs, the most important finding was the negligible level of erucic acid (C22:1n9) below the detection limit. Oleic acid (C18:1n9) proportion was generally high (~50) and significantly decreased in treated groups. Oil quality affection highlights the importance of effective pest management to maintain the nutritional and technological value of rapeseed, as shifts in the n6:n3 ratio and thrombogenic index reflect stress responses rather than agronomic benefits.

Seeds doi: 10.3390/seeds5010010

Authors: Ahmad Jawad Shahbaz Hussain Muhammad Zubair Akram Asif Ameen Atif Naeem Madad Ali Samreen Nazeer

Direct seeded rice, being less water- and labor-intensive, can be an alternative approach to conventional rice planting methods. However, uneven and poor stand establishment caused by deep sowing in the field is one of the major hurdles in the adoption of direct seeding technology. Varieties with the potential to emerge from deeper layers of soil may have a positive impact on crop establishment. To evaluate the behavior of ten rice cultivars against their potential to emerge from different soil depths (0, 2.5, and 5.0 cm), a pot experiment was conducted under semi-controlled conditions at the PARC Rice Programme, Kala Shah Kaku, Lahore. Data on different seedling parameters were collected. The results showed that the highest mean seedling emergence percentage (95%) was achieved by the tested genotypes at a 2.5 cm seeding depth, while surface sowing and placement of seeds at a 5 cm depth demonstrated a similar mean emergence percentage (89%). Seeding depth, genotypes, and their interactions significantly affected mean emergence time, mesocotyl and coleoptile lengths, and root and shoot lengths. Sowing seeds at a 5 cm depth increased mean emergence time by 28%. However, increasing sowing depth increased the coleoptile length, mesocotyl length, first leaf sheath length, and shoot length of rice seedlings. Mesocotyls and coleoptile lengths showed a linear relationship with mean emergence time. Mesocotyl and coleoptile are key structures of the apical–basal axis in grasses that elongate to facilitate the emergence of germinating seeds under deep sowing. The longest coleoptiles (1.47 cm) and mesocotyls (3.27 cm) were measured from seedlings sown at a depth of 5 cm. Among genotypes, PK-1121 exhibited maximum coleoptile elongation (2.10 cm) under deep sowing (5 cm), while the longest mesocotyls were recorded from deep-sown (5 cm) seedlings of Chenab Basmati. Root length was found to be inversely proportional to sowing depth. PK-1121 aromatic, Kisan Basmati, Punjab Basmati, and Chenab Basmati produced longer shoots (22.61, 23.37, 23.32, and 21.05 cm, respectively) and took a relatively short time for emergence when sown deep. These varieties may have better potential to emerge from deeper soil layers, which may have a positive impact on even germination and better crop stand establishment.

Seeds doi: 10.3390/seeds5010009

Authors: Bailey Parkhouse Hannah Chan Wallace Martin Meyer

California sage scrub is an endangered, shrub-dominated, southern California ecosystem threatened by increasing fire frequencies and type-conversion to non-native grasslands. Once non-native grasses become established, their presence promotes more frequent fires, perpetuating grass dominance. To better understand how fire influences soil seed bank assemblages, we examined soil seed banks in burned and adjacent unburned sage scrub at the Robert J. Bernard Field Station (BFS) in two areas that burned in September 2013 and May 2017. In contrast to a previous soil seed bank study in California sage scrub, we found that unburned soil seed banks in sage scrub at the BFS were primarily composed of native seeds (88% of sprouts in unburned areas were native), highlighting that soil seed bank dynamics differ among California sage scrub sites. Despite burned areas supporting elevated densities of non-native seeds (the majority of which included Festuca myuros, a non-native grass), soil seed banks in our burned areas retained native seeds (21% of sprouts in burned areas were native), including native shrub species, suggesting that not all sage scrub habitats are primed to transition to non-native grasslands following disturbances. However, elevated densities on non-native seedlings in burned areas highlight the vulnerability of sage scrub to fire disturbances and the subsequent establishment of non-native grasses.

Seeds doi: 10.3390/seeds5010008

Authors: Aikaterini Papanikolaou Maria Irakli Konstantinos Kampas Chrysanthi Pankou Irini Nianiou-Obeidat Athanasios G. Mavromatis

Chickpea (Cicer arietinum L.) is an important legume, valued for its nutritional and bioactive components. In this study, seven chickpea advanced breeding lines, an elite line, and a cultivar were evaluated under field conditions to assess superior agronomic performance, seed quality traits, nutritional composition, and phenolic profile. A combined approach was used, integrating field phenotyping, seed quality assays, and LC–MS-based phenolic profiling. Significant genotype-dependent variation was observed in plant height, biomass yield, and 1000-seed weight, with P9/14 and P10/14 advanced lines performing strongly in yield-related traits. Seed functional properties also differed, with P8/14 showing superior hydration and seed coat characteristics, while cv. Blanco Sinaloa exhibited the highest hydration and swelling capacities. Protein content ranged from 22.6% to 25.4%, with P9/14 being the most protein-rich advanced line. Phytochemical and antioxidant analyses revealed substantial differences among genotypes: Blanco Sinaloa and M-15370 showed the highest total phenolics and ABTS activity, whereas P14/14 exhibited the strongest DPPH scavenging capacity. LC–MS profiling identified six major phenolic subclasses, with isoflavones predominating and biochanin A and its derivatives being the most abundant compounds. Overall, the integration of agronomic, nutritional, and phytochemical data highlights the advanced lines P14/14 and P9/14 as promising candidates for future breeding programs aimed at enhancing chickpea nutritional quality and functional seed attributes.

Seeds doi: 10.3390/seeds5010007

Authors: Norman F. Weeden Giseli Valentini

Two well-known recessive mutations (a, conditioning white flowers and unpigmented testa; and r, conditioning wrinkled seeds) were found to be major contributors to the loss of germination percentage in garden pea (Pisum sativum L.) when seeds were maintained at cool temperatures (5 °C) for extended periods. After approximately 20 years in storage, seeds homozygous for the unpigmented mutation displayed an average germination rate about 20% lower than wildtype seeds, while wrinkled seeds displayed a rate about 25% less. Seeds homozygous for both the a and r mutations (a combination typical of many commercial cultivars) exhibited a reduction in germination percentage of about 50% over the storage period, indicating that the two mutations have an additive effect on the ageing process. Additional results involving a second mutation (a2) in the phenylpropanoid pathway, as well as information available from the literature that a second, independent mutation in starch synthesis (rb) also reduces seed longevity, suggest that an intact phenylpropanoid pathway and a normally functioning starch synthesis pathway are necessary for optimal storage life of pea seeds.

Seeds doi: 10.3390/seeds5010006

Authors: Ana Minerva García-Cervantes Mohamed Ezzaitouni Tarik Chileh-Chelh Salima Haddou Ferdaous Al Ferjani José Luis Guil-Guerrero

Wild seeds constitute a taxonomically diverse and underexplored reservoir of C18-series bioactive fatty acids (BFAs) with significant nutritional, biomedical, and industrial relevance. This review integrates current knowledge on their lipid composition, metabolic architecture, and potential applications. Numerous wild taxa accumulate high levels of oleic, linoleic, α-linolenic, γ-linolenic, and stearidonic acids, while others synthesise structurally specialised compounds such as punicic, petroselinic, and sciadonic acids. These FAs, together with tocopherols, phytosterols, and phenolics, underpin antioxidant, anti-inflammatory, immunomodulatory, and cardiometabolic effects supported by in vitro and in vivo evidence. The occurrence of these unusual lipids reflects lineage-specific modulation of plastidial and endoplasmic-reticulum pathways, including differential activities of SAD, FAD2/3, Δ6- and Δ5-desaturases, elongases, and acyl-editing enzymes that determine the final acyl-CoA and TAG pools. Wild seed oils show strong potential for translation into functional foods, targeted nutraceuticals, pharmacologically relevant lipid formulations, cosmetic ingredients, and bio-based materials. However, their exploitation is constrained by ecological sustainability, oxidative instability of PUFA-rich matrices, antinutritional constituents, and regulatory requirements for novel lipid sources. This review positions wild seeds as high-value, underused lipid resources with direct relevance to health and sustainability. It underscores their potential to enhance nutritional security and offer alternatives to conventional oil crops.

Seeds doi: 10.3390/seeds5010005

Authors: Felipe de Campos Carmona Abdelbagi M. Ismail James Egdane Gustavo Soares Lima Ibanor Anghinoni Sidnei Deuner Filipe Selau Carlos

Soil salinity is a major abiotic stress that limits rice production, with severity varying among genotypes. It disrupts key physiological processes, particularly water uptake and membrane integrity. This study evaluated six rice genotypes to (i) determine the critical salinity threshold for seed germination and (ii) investigate the physiological mechanisms underlying genotypic variation. Seeds were exposed to saline solutions of up to 32 dS m−1 under controlled conditions, and germination was recorded at 2, 5, 10, and 14 days after stress imposition. Additional assays at 0, 12, 18, and 24 dS m−1 for 1, 3, and 5 days assessed water uptake, electrolyte leakage, and malondialdehyde (MDA) accumulation. The critical threshold for germination was consistent across genotypes (26.01–28.53 dS m−1), except for Nona Bokra, which was more sensitive (20.5 dS m−1). Salinity reduced seed water uptake and promoted membrane degradation, as evidenced by increased electrolyte leakage and MDA accumulation, with severity proportional to stress duration.

Seeds doi: 10.3390/seeds5010004

Authors: Siwakorn Ponkham Kanogwan Seraypheap

Thai eggplant (Solanum melongena L. cv. Chao Phraya), a widely cultivated vegetable with increasing global demand, is highly susceptible to salinity stress, which can severely impair seed germination and early seedling development. This study investigated the effects of sodium nitroprusside (SNP), a nitric oxide (NO) donor, on seed germination and seedling growth under salt stress conditions. Seeds were pre-treated with SNP at concentrations of 0, 0.05, 0.1, and 0.2 mM for 24 h and subsequently germinated under saline conditions with NaCl solutions (0, 100, and 200 mM). SNP pre-treatment, particularly at 0.05 and 0.1 mM, significantly improved germination percentage and germination rate in seeds exposed to 200 mM NaCl compared to untreated controls. Increased NaCl concentrations induced oxidative stress in seedlings, as evidenced by elevated hydrogen peroxide (H2O2) accumulation, which in turn caused lipid peroxidation, reflected by higher malondialdehyde (MDA) levels. Salt stress significantly increased ascorbate peroxidase (APX) activity, whereas catalase (CAT) activity showed no significant change across treatments. Correlation analysis revealed that APX activity was positively correlated with oxidative stress markers (H2O2) and delayed germination (T50/MGT), whereas CAT activity showed no significant correlation with these parameters. In contrast, elevated APX activity was strongly and negatively correlated with overall seedling growth and vigor (SVI/GI), indicating that the underlying stress condition had a detrimental effect on plant performance. Overall, SNP pre-treatment, particularly at 0.05 and 0.1 mM, significantly enhanced salt tolerance by promoting germination (increasing GP and reducing T50/MGT) and improving seedling growth (SL and RL). This protective effect is associated with improved redox regulation and partial mitigation of oxidative damage, as reflected by changes in H2O2, MDA, and APX; however, excessive SNP concentrations may exert phytotoxic effects, highlighting the importance of optimal dosing.

Seeds doi: 10.3390/seeds5010003

Authors: Hailey Haddock Fernando Hugo Oreja

Morningglories (Ipomoea lacunosa, I. hederacea, and I. purpurea) are persistent, problematic weeds in summer row crops throughout warm-temperate regions. Their vining growth habit and enduring seedbanks lead to recurring infestations and harvest interferences. This review synthesizes current knowledge on the seed ecology of these species to clarify how dormancy, germination, and emergence processes contribute to their persistence. Published anatomical and ecological studies were examined to summarize dormancy mechanisms, environmental signals regulating dormancy release, germination requirements, and seasonal emergence patterns. Morningglories exhibit a dormancy system dominated by physical dormancy, occasionally combined with a transient physiological component. Dormancy release is promoted by warm and fluctuating temperatures, hydration–dehydration cycles, and long-term seed-coat weathering. Once permeable, seeds germinate across broad temperature ranges, vary in sensitivity to water potential, and show limited dependence on light. Field studies indicate extended emergence windows from late spring through midsummer, especially in no-till systems where surface seeds experience strong thermal and moisture fluctuations. Despite substantial progress, significant gaps remain concerning maternal environmental effects, population-level variation, seedbank persistence under modern management, and the absence of mechanistic emergence models. An improved understanding of these processes will support the development of more predictive and ecologically informed management strategies.

Seeds doi: 10.3390/seeds5010002

Authors: Faramarz S. Gard Emilia B. Halac Eleonora F. Espeleta Paula N. Alderete Brian E. Robertson Ailin Glagovsky Guadalupe Murga Karina B. Balestrasse Leandro Prevosto

Low-temperature plasma treatments were applied to barley seeds using a dielectric barrier-stabilized corona discharge operated in ambient air enriched with oxygen or nitrogen to quantify surface chemical modifications and seed wettability. X-ray photoelectron spectroscopy showed that oxygen-enriched plasma produced the strongest oxidation, increasing surface oxygen from 9 ± 5 at% (control) to 24 ± 5 at%, while reducing carbon from 88 ± 5 at% to 76 ± 5 at%. Nitrogen-enriched plasma induced more moderate changes (O: 13 ± 5 at%, C: 85 ± 5 at%) but resulted in clear nitrogen incorporation, with an enhanced N 1s amine/amide component at ~400.8 eV. The hydroxyl O 1s contribution increased from 70% (control) to 82% (oxygen) and 90% (nitrogen), indicating substantial surface hydroxylation. SEM-EDX showed only minor micrometer-scale composition changes and no detectable morphological damage. Raman and ATR-FTIR spectra confirmed that polysaccharide, protein, and lipid structures remained intact, with intensity variations reflecting increased hydrophilicity. Water imbibition kinetics fitted with the Peleg model demonstrated faster initial hydration after plasma exposure, with 1/k1 increasing from 20.25 ± 1.90 h−1 (control) to 36.70 ± 6.56 h−1 (oxygen) and 38.87 ± 7.57 h−1 (nitrogen), while 1/k2 remained nearly unchanged.

Seeds doi: 10.3390/seeds5010001

Authors: Bonface O. Manono

Soil salinity poses a major threat to agriculture by severely limiting how well plants grow and produce crops. It strongly inhibits seed germination, a critical stage for plant life. Thus, it is critical to understand the complex ways salinity affects seed germination at the physiological, biochemical, and molecular levels to develop effective salt stress mitigation strategies. This review synthesizes the underlying mechanisms of how salinity inhibits seed germination, the observed impacts of this inhibition, and potential mitigation strategies. The review revealed that high salt concentrations reduce seed germination percentage and increase germination time through multiple mechanisms. They create osmotic stress that reduces water uptake, cause ion toxicity that disrupts critical metabolic activities, and induce oxidative stress. Furthermore, salinity can modify endogenous hormonal profiles, specifically by decreasing germination stimulants like gibberellic acids while increasing inhibitors like abscisic acid. The review finally explored the strategies to mitigate salinity’s adverse effects on seed germination. They include seed priming, a technique involving partial hydration of seeds in an eliciting solution, a promising biotechnological tool to overcome salinity problems during seed germination. Other approaches are the use of organic amendments and the breeding of salt-tolerant varieties. Future research should combine conventional and advanced molecular technologies to develop salt-tolerant cultivars to ensure food security in salt-affected agricultural lands.

Seeds doi: 10.3390/seeds4040069

Authors: Ada S. Vélez Brenda L. Fina Juan A. Arguello Matías G. Ferreyra Brenda Santamaría Pablo S. Vélez Carla G. Zilli Leandro Prevosto Karina B. Balestrasse

Chickpea seed quality is highly susceptible to mechanical damage during handling and to rapid deterioration under postharvest storage. Atmospheric pressure Non-Thermal Plasma (NTP) has shown positive effects on seed quality in several species, but its long-term impact on chickpea remains poorly understood. This study evaluated the effect of NTP on the physiological germination process and postharvest deterioration of Cicer arietinum L. (Fabaceae) ’Felipe UNC-INTA’ seeds. Seeds were treated for three minutes with dielectric barrier discharge using O2 and N2 as carrier gases. Results showed that NTP optimized the triphasic germination response in embryo, especially in phases II and III, where radicle protrusion occurred earlier in treated (27 and 30 h) than in control (33 h) seeds, accompanied with a partition ratio < 1, indicating the roots’ preferential assimilate allocation. Fungal incidence decreased notably, e.g., Aspergillus decreased from 31% (control) to 11% (N2) and 10% (O2). O2-treated seeds exhibited higher germination (94%) than the control (90%) and an 11% reduction in individual electrical conductivity, indicating enhanced membrane integrity. After six months of storage, both treatments delayed aging, maintaining higher vigor than untreated seeds. Overall, NTP emerges as a promising postharvest technology to enhance and preserve seed vigor and viability in C. arietinum.

Seeds doi: 10.3390/seeds4040068

Authors: Daynara Martins da Silva Tathiana Elisa Masetto Leilaine Gomes da Rocha Valdiney Cambuy Siqueira Diogo Santos Crippa Allan Dellon Alegre Takagi

Soybeans are widely used in agro-industrial sectors, and global demand for this crop continues to rise. After harvest, however, soybean seeds often lack the appropriate moisture content for storage, making drying a common practice under changing climate conditions. Because temperature is a critical factor during drying, this study aimed to evaluate the effect of air-drying temperature on physiological responses and cytogenetic conformation of soybean seeds. The experiment was conducted under a completely randomized design with four replications for each temperature. Seeds with 23 percent moisture content were dried in a convective dryer equipped with airflow and temperature control at 40 °C, 50 °C, 60 °C, and 70 °C until reaching 13 percent. Samples for physiological and cytological analyses were collected before and after drying. The results indicated that drying temperature influenced seed performance and vigor. Moreover, nuclear alterations were identified as an important component of the genotoxicity caused by high drying temperatures. Overall, air temperatures above 50 °C induced physiological and cytogenotoxic effects, underscoring the need for careful monitoring during seed drying.

Seeds doi: 10.3390/seeds4040067

Authors: Elhoucine Elfatimi Recep Eryiğit Lahcen Elfatimi

Agricultural research has accelerated in recent years, but farmers often lack the time and resources to conduct on-farm experiments, as most of their efforts are devoted to crop production. Seed classification provides essential insights for seed quality control, impurity detection, and yield estimation. Early identification of seed types is critical to reduce costs, minimize risks of poor field emergence, and support efficient crop management. Traditional classification methods rely heavily on manual feature extraction and expert input, which limits scalability and accuracy when dealing with highly similar seed types. To address this challenge, we propose an automated end-to-end deep learning framework for complex multiclass Brassica seed classification. Our framework integrates preprocessing, feature learning, and classification into a unified pipeline, eliminating the need for handcrafted features. Using a newly collected dataset of ten Brassica seed classes characterized by high texture similarity, we develop and evaluate a convolutional neural network optimized through architectural design and hyperparameter tuning. Experimental results demonstrate that the proposed framework achieves a classification accuracy of 93%, outperforming several state-of-the-art pretrained models. These findings highlight the potential of automated end-to-end deep learning models to enhance precision agriculture, providing robust and scalable solutions for seed quality monitoring and agricultural productivity.

Seeds doi: 10.3390/seeds4040066

Authors: Aspasia Efthimiadou Nikolaos Katsenios Lida Papalamprou Varvara Andreou Sofia Chanioti Marianna Giannoglou Ioanna Kakabouki George Katsaros

Sprouts are gaining popularity among consumers worldwide due to their high nutritional properties. A comparative evaluation of novel and environmentally friendly pre-sowing seed treatment techniques was conducted to enhance wheat sprout production. Pulsed electromagnetic field (PEMF), cold atmospheric plasma (CAP), and high-pressure processing (HP) at 200 and 600 MPa were applied on durum wheat seeds for 3 and 10 min. The above techniques, along with ozonation (OZ), were also applied for 3 and 10 min for the “activation” of water that was used for immersion of the wheat seeds. Seed germination percentage, root and shoot length, and seedling dry weight were the measurements for the comparative evaluation of 21 treatments of seeds growing in Petri dishes. The results indicated that CAP, PEMF, and OZ treatments had positive effects on wheat sprout production, while prolonged exposure to HP processing appeared to stress the seeds. Overall, the multiple comparisons of four processing technologies, applied by two methods and at two exposure times, could be a benchmark study for further understanding the response of seeds in pre-sowing techniques.

Seeds doi: 10.3390/seeds4040065

Authors: Emmanuel Chukwueloka Ugwu Issah Sugri Mathieu A. T. Ayenan Agyemang Danquah Eric Yirenkyi Danquah

Bambara groundnut is an underutilized legume with significant potential for enhancing nutrition and food security. However, limited knowledge of its seed systems hinders the development of targeted interventions. This study assessed the Bambara groundnut seed systems and seed quality in Ghana. A semi-structured questionnaire was used to examine seed production, conditioning, and saving practices, while 150 farmer-saved seed samples were evaluated for quality. The findings revealed that the seed system is predominantly informal, with 99% of farmers relying on their own saved seeds, which showed poor germination and emergence. About 54.4% sourced seeds from local markets, and 60.7% recycled seeds for five years or more. Seeds were stored unshelled in polypropylene bags in Tolon and shelled in the Kintampo South and Talensi Districts. Seed selection was primarily based on size (in Tolon) and visible absence of disease symptoms (in Kintampo South and Talensi). An incidence (7.6%) of seed-borne pathogens was recorded, with Aspergillus flavus (38.8%) and A. niger (16.6%) being most prevalent. Other pathogens included Macrophomina phaseolina (11.5%), Rhizopus spp. (6.5%), Curvularia lunata (5.3%), and A. fumigatus (1.9%). This study highlights the need to support community-based seed systems to improve farmers’ access to quality Bambara groundnut seed.

Seeds doi: 10.3390/seeds4040064

Authors: Jose Ricardo Bagateli Ricardo Mari Bagateli Giovana Carla da Veiga Ivan Ricardo Carvalho Willyan Junior Adorian Bandeira Geri Eduardo Meneghello

Soybean seed production in low-altitude subtropical environments in Paraguay is constrained by the antagonism between achieving high grain yields and high physiological seed quality, a relationship governed by the Genotype x Environment x Management (GxExM) interaction. This study aimed to elucidate this trade-off by evaluating three sowing dates (early, normal, late) across genotypes from three relative maturity groups over four growing seasons. Our results demonstrate that sowing date is the primary factor modulating this antagonism. Early and normal sowings maximized yield (up to 62.8% higher than late sowing) by synchronizing the reproductive period with maximum solar radiation but exposed the maturation phase to severe thermal and water stress. This consistently resulted in a high incidence of green seeds (>95% higher than late sowing) and a drastic reduction in seed vigor, with longevity potential reduced by up to 63.6%. In contrast, late sowing functioned as a stress-escape strategy, shifting maturation to milder autumn conditions and consistently producing seeds of high vigor, viability, and greater longevity. Critically, during a season of extreme drought and heat, the late sowing not only preserved quality but also produced the highest grain yield, highlighting its decisive role in risk mitigation. Thus, sowing date is a key management tool for targeted production in these environments.

Seeds doi: 10.3390/seeds4040063

Authors: Antonios Mavroeidis Panteleimon Stavropoulos Ioannis Roussis Stella Karydogianni George Papadopoulos Stavroula Kallergi Ioanna Kakabouki Vasiliki Pachi Vassilios Triantafyllidis Eleni C. Mazarakioti Angelos Patakas Anastasios Zotos Eleni Tsiplakou Dimitrios Bilalis

Stinging nettle (Urtica dioica L.) is a multipurpose perennial plant with growing interest as a source of nutrients for both human and animal consumption. Despite its recognized nutritional potential, limited research has addressed how agronomic practices influence its nutritional quality. The aim of the present study was to assess the effects of plant density and fertilization on the micro- and macronutrient content of nettle plants and seeds. A three-year field experiment (2021–2024) was conducted under Mediterranean conditions using a split-plot design with two plant densities (12 and 16 plants m−2) and three nitrogen fertilization regimes [control (0 kg N ha−1), urea (200 kg N ha−1), and urea with urease inhibitor (200 kg N ha−1)]. Results showed that nitrogen fertilization significantly affected macronutrient composition, increasing crude protein and crude carbohydrates (by up to 6% and 4% respectively) while reducing crude fat and fiber contents by up to 10% in nettle plants. However, fertilization negatively influenced the concentrations of Mg, Zn, K, and Mn in seeds and reduced their content by up to 16%, 4%, 9%, and 5% respectively. On the contrary, Fe and Cr increased under nitrogen application. Plant density had a minor effect on nutritional content, mainly improving protein accumulation in lower densities. Overall, U. dioica demonstrated a stable nutritional profile and high mineral content, therefore supporting its potential as a sustainable dual-purpose crop for food and feed systems. The findings of the present study indicate that proper crop management can significantly improve the nutrient content of nettle plants and seeds.

Seeds doi: 10.3390/seeds4040062

Authors: Saubhagya Subhadarsini Sahoo Dwipak Prasad Sahu Rajendra Kumar Behera

Drought is a major abiotic stress that compromises seed germination, seedling establishment, and subsequent crop productivity, thereby threatening agricultural sustainability and food security. Developing effective seed-based strategies is therefore essential to enhance drought resilience. In this study, we investigated the efficacy of green-synthesized silver nanoparticles (AgNPs), produced using Azadirachta indica (neem) flower extract as a seed priming agent, to improve drought tolerance and early growth in wheat (Triticum aestivum). Seeds were primed with AgNPs (25–100 mg L−1), PEG 6000 (−0.6, −0.8, and −1.0 MPa), and their combination (AgNPs + PEG 6000). AgNP priming enhanced germination by 72%, 86%, and 100% at 25, 50, and 75 mg L−1, respectively, compared with the control, with 75 mg L−1 identified as the optimal concentration. This treatment increased total chlorophyll and carotenoid contents by 14% and 6%, and elevated phenolic and flavonoid accumulation by 58% and 97%, respectively. Antioxidant enzyme activities were also substantially increased—catalase (CAT) by 44%, superoxide dismutase (SOD) by 23%, peroxidase (POX) by 11%, and glutathione reductase (GR) by 58%. Under drought stress, AgNP priming at 75 mg L−1 improved germination by up to 80%, indicating enhanced drought tolerance. Elevated protein and antioxidant enzyme levels, along with reduced malondialdehyde (MDA), proline, and total soluble sugar levels, further confirmed mitigation of oxidative stress. Collectively, these results demonstrate that neem-mediated green-synthesized AgNPs could serve as an effective seed-priming agent, promoting wheat seedling establishment and enhancing drought resilience under water-deficit conditions.

Seeds doi: 10.3390/seeds4040061

Authors: Balázs Szemerits Gábor Kukorelli Wogene Solomon Kabato Zoltán Molnár

Efficient and climate-resilient Sorghum bicolor L. cultivation is increasingly important under Central European conditions. This study evaluated two hybrids across two locations in 2023–2024 with row spacings of 25, 45, and 75 cm and four sowing densities of 210,000–300,000 seed ha−1. Row spacing, year, and genotype exerted a strong and consistent effect on grain yield and quality, with multiple instances reaching high statistical significance (p < 0.001). In contrast, seed rate had no significant main effect, influencing results only via site- and season-specific interactions. At a 45 cm row spacing, sorghum exhibited the highest grain yield (8.59 t ha−1), the lowest seed moisture content (13.59%), and the greatest protein yield (1.094 t ha−1). The 25 cm spacing with higher density produced with 0.46 t ha−1 higher yields than 75 cm and the highest protein content (13.35%), but 0.48 t ha−1 lower yield than the 45 cm treatment. The 75 cm spacing generally gave 12.29% lower yield and 6.72% lower quality despite higher tillering. TKW was highest at 45 cm row spacing (31.12 g), 23.3% greater than at 75 cm (25.25 g). The 45 cm row spacing provided the most stable yield, superior quality, and balanced agronomic performance, representing the most practical configuration for sustainable sorghum production under Central European conditions.

Seeds doi: 10.3390/seeds4040060

Authors: Elmer Gonçalves Josiane Carvalho Caris Viana Pedro Santos Katharine Gonçalves Karen Costa Auxiliadora Martins Silvana Silva Roberval Lima Patrícia Albuquerque Andreia Fernandes Wagner Araújo José Francisco Gonçalves

Given the reduced resilience of the Amazon rainforest due to deforestation, identifying high-quality genetic markers for the propagation of native species is crucial for forest regeneration. This study investigated metabolic dynamics during Brazil nut (Bertholletia excelsa) germination to identify biochemical markers for selecting superior genotypes. We analyzed primary reserves (carbohydrates, lipids, proteins) and minerals in two genotypes, 606 and Santa Fé, in seven germination stages. Our results revealed distinct metabolic patterns. Genotype 606 showed 101.73% greater efficiency in the transient accumulation of starch, 34.86% higher degradation of lipids, and 34.86% higher transitory synthesis of soluble proteins. Conversely, Santa Fé was 16.8% more efficient in amino acid synthesis and 795.33% in boron compartmentalization, though less so in sucrose (2.17%) and in lipid synthesis (24.84%). Overall, early germination stages involved starch, sucrose degradation and mineral consumption. During post-germinative stages, protein and lipid degradation likely fueled gluconeogenic pathways and supported carbohydrate synthesis and seedling growth. This work increases the knowledge on Brazil nut germination physiology and identifies metabolic markers that differentiate genotypes. These findings are fundamental for our understanding of primary metabolism turnover in B. excelsa and provide a basis to support forest restoration and genetic improvement programs. In addition, we hope to contribute to the selection of superior high-performance genotypes, which are essential for recovering degraded areas and enhancing productive plantations in the Amazon region.

Seeds doi: 10.3390/seeds4040059

Authors: Karishma Kumari Kwanghee Won Ali M. Nafchi

Cover crop mixes play an important role in enhancing soil health, nutrient turnover, and ecosystem resilience; yet, maintaining even seed dispersion and planting uniformity is difficult due to significant variances in seed physical and aerodynamic properties. These discrepancies produce non-uniform seeding and species separation in drill hoppers, which has an impact on stand establishment and biomass stability. The thousand-grain weight is an important measure for determining cover crop seed quality and yield since it represents the weight of 1000 seeds in grams. Accurate seed counting is thus a key factor in calculating thousand-grain weight. Accurate mixed-seed identification is also helpful in breeding, phenotypic assessment, and the detection of moldy or damaged grains. However, in real-world conditions, the overlap and thickness of adhesion of mixed seeds make precise counting difficult, necessitating current research into powerful seed detection. This study addresses these issues by integrating deep learning-based computer vision algorithms for multi-seed detection and counting in cover crop mixes. The Canon LP-E6N R6 5D Mark IV camera was used to capture high-resolution photos of flax, hairy vetch, red clover, radish, and rye seeds. The dataset was annotated, augmented, and preprocessed on RoboFlow, split into train, validation, and test splits. Two top models, YOLOv5 and YOLOv7, were tested for multi-seed detection accuracy. The results showed that YOLOv7 outperformed YOLOv5 with 98.5% accuracy, 98.7% recall, and a mean Average Precision (mAP 0–95) of 76.0%. The results show that deep learning-based models can accurately recognize and count mixed seeds using automated methods, which has practical applications in seed drill calibration, thousand-grain weight estimation, and fair cover crop establishment.

Seeds doi: 10.3390/seeds4040058

Authors: Le Thi My Thu Tran Trong Khoi Nguyen Dang Le Ngoc Han Nguyen Duc Trong Le Thanh Quang La Cao Thang Tran Chi Nhan Ly Ngoc Thanh Xuan Nguyen Quoc Khuong

Although purple non-sulfur bacteria (PNSB) have been studied as good biofertilizers, their direct effects on maize seed vigor remain unclear. Additionally, the seedling stage is a vital factor for the later growth of maize. This study was conducted to evaluate the effectiveness of potassium-solubilizing PNSB (K-PNSB) in enhancing the vigor of hybrid maize seeds. A completely randomized design was employed, incorporating single strains, Luteovulum sphaeroides M-Sl-09, Rhodopseudomonas thermotolerans M-So-11, and Rhodopseudomonas palustris M-So-14, as well as a mixture of all three strains. Each was tested at bacterial suspension dilution ratios with sterile distilled water of 1:2000; 1:2250; 1:2500; 1:2750; and 1:3000 (v/v), with three replications per treatment. Each replicate consisted of a Petri dish containing 10 hybrid maize seeds of each hybrid of LVN 10, C.P. 511, and NK7328 Gt/BT, and was incubated for five days. The results showed that K-PNSB significantly enhanced root and shoot development compared to the control (p < 0.05). The 1:2500 dilution of the individual strains and the mixture notably improved germination rate, root length, shoot length, and seedling vigor index compared to the control. At the 1:2500 dilution, the improved vigor index increased by 73.5% for L. sphaeroides, 48.7% for R. thermotolerans, 47.4% for R. palustris, and 78.5% for the mixed inoculum in the LVN 10 hybrid. Similar trends were observed for C.P. 511 and NK7328 hybrids, confirming strain- and hybrid-specific responses. The findings highlight that K-PNSB can serve as effective bio-priming agents to enhance maize seed vigor through mechanisms related to potassium solubilization and phytohormone production. Field-scale validation is recommended to assess their long-term agronomic potential.

Seeds doi: 10.3390/seeds4040057

Authors: Thuy Linh Ha Masatoshi Yoshimura Itaru Sotome

Ultrafine bubble (UFB) priming has recently emerged as a promising technique to enhance seed germination, yet its physiological mechanisms remain unclear. This study investigated the effects of UFB priming on adzuki bean (Vigna angularis) seeds under stress-free and drought conditions. Four treatments were tested: Control (non-primed), DW (primed with distilled water), UFB1 (4.56 × 107 particles/mL), and UFB2 (1.13 × 108 particles/mL). For priming, seeds were immersed in each solution at 25 ± 1 °C for 24 h. Seed traits, including water uptake, coat thickness, and total reactive oxygen species (ROS) levels in the priming solutions and seeds, were analyzed. UFB priming reduced seed coat thickness and slowed early water uptake without affecting final moisture content. Total ROS levels displayed a concentration-dependent pattern, with higher UFB increasing ROS in water but reducing them on the seed surface. Under stress-free conditions, all priming treatments accelerated germination, although higher UFB slightly prolonged T50. Under drought simulated by polyethylene glycol (PEG 10 and 15%), non-primed seeds were strongly inhibited, whereas primed seeds maintained high germination rates (>97%). Our results demonstrated that UFB priming improved water uptake, seed coat structure, and ROS signaling, enhancing seed performance of adzuki bean under favorable and drought conditions.

Seeds doi: 10.3390/seeds4040056

Authors: Seung Youn Lee Kyu Seong Choi Chung Ho Ko Yong Ha Rhie

This study aimed to develop seed-based mass propagation techniques for the conservation and horticultural and medicinal uses of Scrophularia koraiensis Nakai, an endemic plant in the Korean Peninsula. Seeds were collected from four different locations (accessions) and subjected to untreated (control), gibberellic acid (GA)-only treatment, cold stratification-only treatment, or a cold stratification + GA treatment. Except for seeds collected from one location, the control group exhibited low germination of below 20% in all other locations. However, the GA-only and cold stratification-only treatments released seed dormancy and promoted germination compared with the control. In particular, the cold stratification-only treatment at 5 °C for 4 weeks resulted in about 80% germination in all accessions and demonstrated positive effects on germination speed and uniformity. These findings indicated that S. koraiensis seeds exhibit non-deep physiological dormancy (PD). Upon comparing the seed dormancy classes across various species of Scrophularia, native to different continents and countries, we confirmed that PD is very well conserved in the genus Scrophularia. Therefore, the study outcomes will provide fundamental and practical insights into the seed dormancy and germination characteristics of various Scrophularia species for future studies.

Seeds doi: 10.3390/seeds4040055

Authors: Pavel A. Dmitriev Anastasiya A. Dmitrieva Boris L. Kozlovsky

Sunflowers are one of the most important agricultural crops in the world. Given the high importance of sunflower products in the world market and the scale of their cultivation, the introduction of precision farming technologies into its culture can have a significant economic and environmental effect. This study demonstrated the fundamental possibility of developing a technology for rapid, remote, and non-invasive assessment of sunflower seed moisture to determine the optimal timing for desiccation and harvesting. It has been shown that the moisture content of sunflower seeds can be assessed with high accuracy based on the spectral characteristics of the underside of the inflorescences obtained using a hyperspectral camera in the visible and near-infrared range (VNIR) (from 450 to 950 nm). Random forest regression (RFR) was used to predict sunflower seed moisture. The model performed excellently on the training data (R2c = 1.00; MAEc = 0.58; RMSEc = 0.74, MAPEc = 1.29) and with a high performance on the testing data (R2t = 0.98, MAEt = 2.99, RMSEt = 3.28, MAPEt = 12.22). The most significant vegetation indices for determining moisture are CCI, Booch, Datt3, Datt4, LSIRed, modPRI, SR5, TCARI, and TCARI2.

Seeds doi: 10.3390/seeds4040054

Authors: Marcilio Fagundes Maria Isabela Rodrigues Silva Bruno Henrique Silva Mayrink Walisson Kenedy-Siqueira Luiz Henrique Arymura Figueiredo Tatiana Cornelissen

The Neotropical Savanna (Cerrado) is a fire-prone biome characterized by seasonal climate, nutrient-poor soils, and variable fire regimes. While fire-induced germination responses are well documented in Cerrado plants, the role of seed size in mediating thermal tolerance remains poorly understood. Here, we investigate how seed size and fire-related heat treatments influence germination in Hymenaea stigonocarpa, a keystone Cerrado tree species. Specifically, we test the predictions that (i) low to moderate fire temperatures (<270 °C) do not impair seed germination and (ii) larger seeds exhibit greater heat tolerance than smaller seeds. We exposed 360 seeds from 30 individual trees to five heat-shock treatments (27, 100, 150, 200, and 270 °C) simulating fire intensities typically experienced in the Cerrado. Our results show that H. stigonocarpa produces relatively large seeds with an average germination rate of approximately 42%. The average time required for germination was 12.18 ± 0.43 (average ± standard error) days. The time required for seed germination varied significantly as a function of heat-shock treatment and seed mass, with seeds exposed to the highest temperature (270 °C) taking longer to germinate. Moreover, seed mass had a positive effect on the time required for seed germination. The germination percentage remains stable across heat treatments and seed sizes, indicating that H. stigonocarpa seeds exhibit characteristics typical of heat-tolerant species rather than those of heat-stimulated species. Our study showed that H. stigonocarpa trees produce large seeds that germinate quickly and are tolerant to moderate temperatures. These seed traits play a crucial role in the reproductive success of individual plants in fire-prone, nutrient-poor, and water-limited ecosystems. Furthermore, our results offer important guidance by emphasizing the role of seed size in effective restoration initiatives.

Seeds doi: 10.3390/seeds4040053

Authors: Violeta Nikolova Nikolay Nikolov Todorka Petrova Venelina Popova Zhana Petkova Milena Ruskova Olga Teneva

A sustainable economy and the drive to reduce agro-industrial waste worldwide motivate the increased interest in alternative uses of traditionally cultivated plants such as tobacco. Tobacco seeds are an underutilized resource with enormous potential for application in various areas of human life. The present study aims to characterize the phytochemical composition and nutritional potential of Oriental tobacco seeds grown in Bulgaria, in order to support their possible application in areas outside the tobacco industry. Two Oriental tobacco varieties (“Krumovgrad 90” and “Krumovgrad 58”) from three production regions were explored and comparatively evaluated in terms of their physical and chemical indicators, determined by standardized methods. The results showed high protein (22.57–23.84%) and energy content (482–531 kcal/100 g), combined with relatively low carbohydrate levels (3.79–4.03%) and the presence of bioactive compounds, such as polyphenols (288–357 mg GAE/100 g). The seeds contained significant amount of oil (36.31–39.24%), of which the fatty acid profile included 16 identified components, with linoleic (72.0–74.4%), oleic (11.2–13.5%), palmitic (9.6–10.2%), and stearic (1.8–2.5%) acids taking the greatest share. The sterol fraction was dominated by β-sitosterol (43.5–46.8%), followed by sitostanol, campesterol and stigmasterol, with a stable distribution between the samples. The main tocol was γ-tocotrienol (56.5–61.4%), with α-tocotrienol being detected only in one of the varieties (“Krumovgrad 58”, 13.3%). The phospholipid fraction showed variations between the samples, with a dominant presence of phosphatidylinositol (18.0–20.4%). The results from the study confirmed the tangible potential of tobacco seeds as a source of biologically active substances in the development of functional foods and dietary supplements.

Seeds doi: 10.3390/seeds4040052

Authors: Alejandro Santiago Jesus Márquez-Pablo Natalia Celaya-Rojas José María Herranz Pablo Ferrandis

Seed dormancy is a key ecological attribute influencing germination timing and the ability of species to establish in variable environments. This study investigated whether inter-population variability in seed dormancy expression exists in Lonicera etrusca, a Mediterranean shrub known for producing seeds with underdeveloped embryos and multiple dormancy types. Seeds were collected from four geographically and ecologically distinct populations in central Iberia and subjected to a series of germination experiments simulating natural seasonal temperature regimes, stratification treatments, and gibberellic acid application. Across all populations, seeds exhibited morphological dormancy (MD) and varying degrees of morphophysiological dormancy (MPD), including non-deep simple and deep complex types. Despite high intra-specific variability in dormancy expression, no significant differences were found among populations for germination patterns or embryo growth responses. This indicates that dormancy variability is an intrinsic, conserved feature of the species rather than a locally adaptive trait. The homogenization of germination strategies across populations may be facilitated by bird-mediated seed dispersal, promoting gene flow and limiting local selection. These findings support the hypothesis that dormancy polymorphism in L. etrusca reflects a flexible germination strategy that enhances colonization potential across heterogeneous Mediterranean environments, rather than an environmentally induced plastic response.

Seeds doi: 10.3390/seeds4040051

Authors: Chih-Yao Hou Shih-Kao Chou Jong-Shinn Wu Hsiu-Ling Chen Pei-Wen Zhang Chih-Tung Liu Chun-Ping Hsiao Chia-Min Lin

Microbial contamination is the main safety concern of sprouts and seeds are the major source. High concentrations of sanitizers (>10,000 mg/kg) are recommended for effective sanitation. Microbubble (MB) was reported to elevate sanitizer efficacy. Hence, MBs combined with disinfectants, chlorine dioxide (ClO2, 500 ppm), and slightly acidic electrolyzed water (SAEW, 250 ppm), were used to inactivate Salmonella Typhimurium on alfalfa seeds. After fulfilling MBs for 10 min, alfalfa seeds were washed in 10 L of water for 10, 20, or 30 min. Compared with untreated seeds, S. Typhimurium reductions obtained by SAEW-MBs (SMBs) and ClO2-MBs (CMBs) for 20 min were 3.8 and 3.3 log CFU/g, respectively. Conversely, the 20 min treatments of SAEW and ClO2 only obtained reductions of 0.9 and 1.1 log CFU/g, respectively. More surface ruptures on the seeds treated with CMBs were observed under a scanning electron microscope compared with the ones treated by water and ClO2 only. No adverse effects on the seed germination rate and the weight yield of sprouts were observed when treated with CMBs for 20 min. An MB device with capacity of 100 L was assembled and achieved reductions of 3.9 and 3.2 log CFU/g of natural microbes and S. Typhimurium, respectively, after 20 min CMB washing. Additionally, an MB device at 250 L was assembled and achieved 3.0 log CFU/g reduction in natural microbes. This study demonstrated that MBs enhanced the efficacy of disinfectants and could be applied in industrial-scale operations.

Seeds doi: 10.3390/seeds4040049

Authors: Jake Cummane William J. W. Thomas Maria Lee Mohammad Sayari David Edwards Jacqueline Batley Aria Dolatabadian

Seed-related traits such as seed size, germination, vigour, dormancy, biochemical composition, and stress resistance are critical to ensuring agricultural productivity and global food security, particularly in current scenarios of climate change and environmental unpredictability. This review examines the transformative potential of omics technologies, encompassing genomics, transcriptomics, proteomics, metabolomics, epigenomics, and phenomics, in enhancing our understanding of seed biology and its applications in crop improvement. Genomics and transcriptomics are key technologies in future plant breeding and gene editing to optimise seed yield and quality. We reviewed the role of metabolomic approaches in uncovering the molecular mechanisms behind seed germination, vigour, dormancy, and the proteomic advances to elucidate markers of seed quality, combining these omic technologies to decipher DOG1 as a marker of dormancy. Both biotic and abiotic stress resistance in seeds were reviewed from a multi-omics perspective to determine the best avenues for improving the resilience of seeds against drought, salinity and pathogens. Moreover, omics approaches have been reviewed to optimise plant–microbe interactions, particularly in enhancing symbiotic relationships within the soil microbiome.

Seeds doi: 10.3390/seeds4040050

Authors: Mahdiyeh Safaripour-Chafi Amalesh Dhar Sarah R. Wilkinson M. Anne Naeth

Research on the remediation of hydrocarbon contaminated peatlands is limited; in particular, hydrocarbon effects on seed germination is critical for effective reclamation. This study examined germination responses of six wetland plant species under greenhouse and laboratory conditions. Seeds were exposed to hydrocarbon-contaminated peat soil and ground water under two light treatments (light, total darkness) for four weeks. Species specific responses in seed germination and germination velocity occurred under different light conditions and exposure to hydrocarbon-contaminated peat soil and water. Light significantly impacted germination, while hydrocarbon-contaminated peat soil and water had no effect. Glyceria grandis (83.5%) and Scirpus microcarpus (74%) had significantly higher germination rates even in contaminated treatments than Carex aquatilis (28%) and Typha latifolia (38%), which had modest germination. Modified Timson’s Index (germination velocity) was significantly greater in Scirpus microcarpus (21.90) and Glyceria grandis (19.20) than in other species after 30 days. Carex utriculata and Scirpus validus had ≤0.5% germination and ≤0.2 velocity. The overall species mean germination time was >9 days with a low (≤0.7) germination index. Ordination using several germination variables separated some species. These findings suggest Scirpus microcarpus and Glyceria grandis have high tolerance to hydrocarbon contamination and may be effective candidates for the phytoremediation and restoration of contaminated peatlands.

Seeds doi: 10.3390/seeds4040048

Authors: Xu Wang Ying Luo Yuan Cao Yujin Gong Francoise Corbineau Yong Xiang

Seed dormancy and germination is regulated by internal hormones and exogenous environment cues. Ethylene is one of the hormones that break seed dormancy and induce seed germination. Our previous study showed that N-degron pathway gene, proteolysis6 (PRT6) was involved in dormancy release by ethylene, the defection of which exhibiting ethylene-insensitivity in Arabidopsis thaliana. In the present study, through screening an ethyl methyl sulfonate-mutagenized (EMS) population of prt6−1, we isolated a recessive mutant that acted as a suppressor of prt6 that rescued its insensitivity to ethylene as well as a phenotype of shorter silique length. Further bulk segregant analysis on F2 population identified a premature termination located in the third exon of LONGIFOLIA1 (LNG1), previously reported in the regulation of longitudinal cell elongation. Mutation of LNG1 in prt6−1 background by CRISPR-Cas9 confirmed that LNG1 was epistatic to PRT6 in seed responsiveness to ethylene. Our finding proposed the pleiotropic effect of LNG1 in seed dormancy breakage by ethylene via PRT6, providing novel functional component at the downstream of the coordinated PRT6 and ethylene signaling pathway.

Seeds doi: 10.3390/seeds4030047

Authors: Erick H. Ochoa-Chaparro Juan J. Patiño-Cruz Julio C. Anchondo-Páez Alan Alvarez-Monge Cristina L. Franco-Lagos Esteban Sánchez

Nanopriming with metal nanoparticles (NPs) is a promising strategy for improving seedling quality in horticultural crops. This study evaluated the effects of hydropriming, ZnO, SiO2, ZnO + SiO2, a ZnMo nanofertilizer, and two commercial biostimulants (Osmoplant and Codasil) on the early development of Capsicum annuum L. seedlings. Morphological, physiological, and biochemical traits, including biomass, stem architecture, number of leaves, chlorophylls, carotenoids, SPAD index, and nitrate reductase (NR) activity, were measured under controlled conditions. The ZnO and ZnO + SiO2 treatments promoted stronger root growth, higher pigment content, and higher NR activity. SiO2 alone and ZnMo showed intermediate improvements, while Osmoplant and Codasil had more limited effects. Multivariate analyses provided complementary information: heat maps revealed correlations between traits, PCA differentiated treatment responses, and radar charts integrated performance profiles. Overall, the results provide promising evidence that seed nanopriming, particularly with ZnO and ZnO + SiO2, improves seedling vigor and transplant potential in jalapeño peppers.

Seeds doi: 10.3390/seeds4030046

Authors: Marco Carli Lorenzo Guglielminetti Thais Huarancca Reyes

Chenopodium quinoa Willd. (quinoa) is a very promising crop due to its nutraceutical properties and strong tolerance to extreme conditions, including high UVB. However, the physiological mechanisms underlying its adaptation to high UVB are still unclear, especially during germination as its traditional sowing consists of either broadcasting or continuous stream distribution in furrows. We evaluated the response of germinating quinoa seeds to acute UVB radiation, looking at the mobilization of starch reserves as well as the utilization of starch and free sugars. Biometric and physiological traits were evaluated in control (0 W m−2) and UVB (3.4 W m−2)-exposed seeds during a 24 h treatment starting with seed imbibition. Quinoa exposed to UVB showed a delay in germination and strong reduction in root elongation. Although the negative effect of UVB on germination was fully recovered at 48 h of imbibition, that on root elongation was irreversible, especially with a longer exposure time. Further analysis showed low differences in the concentration of free sugars, except at 2 and 24 h of treatment. Furthermore, starch mobilization in UVB-treated seeds was strongly reduced compared to control. This was associated with the amylolytic activity analysis, which showed strong reductions in both α- and β-amylase activities during the whole treatment, indicating that UVB strongly reduced enzyme activation for the mobilization and use of starch reserves. Overall, these data suggest that quinoa seeds can regulate the expression of genes encoding enzymes involved in reserve mobilization, in order to resist to acute UVB radiation and maintain seed viability.

Seeds doi: 10.3390/seeds4030045

Authors: Carmen Díaz-Paniagua Rocío Fernández-Zamudio

Aquatic plants may rely on seeds to promote population persistence after severe disturbances, such as droughts. We characterized the seed germination dynamics for three Potamogeton species following seed storage under dry versus submerged conditions. Overall germination levels were highest for P. lucens and, more specifically, were higher after submerged storage (70.4%) than dry storage (56.0%). Overall germination levels were lower for the two other species and displayed a different response to storage conditions; they were higher after dry storage (P. natans: 24.6%; P. pectinatus: 28.1%) than submerged storage (10.8 and 7.1%, respectively). Only P. natans would have likely made a large seed bank contribution as follows: 42.2% of its seeds remained ungerminated and viable after submerged storage, while this figure was 17.4% for seeds that had experienced dry storage. Our results suggest the species differ in their reproductive strategies. Sexual reproduction plays an important role in Potamogeton lucens, adding new individuals to plant populations every year; however, the low viability of the species’ ungerminated seeds suggest its seed bank contribution may be small, rendering it vulnerable to long periods of unfavourable environmental conditions. In contrast, P. natans would likely make a larger seed bank contribution, underscoring the role its seeds may play in population persistence across years. Potamogeton pectinatus may minimally rely on its seeds, which fits with its predominant use of vegetative reproduction in the field. While its seeds might contribute little to population persistence, they may nonetheless promote genetic variability among populations.

Seeds doi: 10.3390/seeds4030044

Authors: Gustavo Echeverri-Jaramillo Flaviano Trasmundi Junior Bernardo Molina-Hernández Chiara Rossi Fabio Stagnari Clemencia Chaves-López

Modern agriculture faces critical challenges from soil degradation and excessive use of chemical fertilizers, threatening sustainable seed germination and crop establishment. The main objective of this research was to evaluate the feasibility of using Pseudomonas spp. isolated from Colombian mangrove sediments as biofertilizers to enhance crop productivity, with an emphasis on Lactuca sativa (lettuce) seed-to-seedling transition. Fifty-eight isolates were characterized and screened for their ability to improve the germination of the lettuce seeds, but only nine strains showed promising activity. The strains were identified at the species level and screened for Plant growth-promoting rhizobacteria (PGPR) traits such as indole-3-acetic acid (IAA) production, phosphate solubilization, organic acid production, and salt tolerance (5%). Four different species were identified, namely Pseudomonas putida (one strain), Pseudomonas monteiilli (two strains), Pseudomonas taiwanensis (one strain), and Pseudomonas aeruginosa (four strains). P. aeruginosa strains P8 and P9 demonstrated exceptional performance, significantly improving root elongation, seedling biomass, and antioxidant activity compared with the uninoculated plants in vitro. These strains showed dual agrobiotechnological value: (i) enhancing early seedling vigor through hormonal stimulation and (ii) improving nutrient availability via phosphate solubilization. Our findings highlight the biotechnological potential of mangrove-associated Pseudomonas spp. as a sustainable alternative to chemical fertilizers, particularly for seed priming and seedling establishment systems in stress-prone soils.

Seeds doi: 10.3390/seeds4030043

Authors: Jacymara Lopes Pereira Geovanna Vitória Olimpio Fernanda Silva Coelho Maria Luiza Carvalho Santos Juliana Lopes Moraes Deise Paes Sara Sangi Amanda Azevedo Bertolazi Alessandro Coutinho Ramos Clícia Grativol

Soybean seeds (Glycine max) are of great economic and nutritional importance due to their high oil and protein content. Seed germination is an essential process that influences crop yield and quality. The seed embryo resumes growth when it imbibes, which induces the expression of genes related to cell expansion. The role of acid growth in the embryonic axis during germination is not well characterized. Thus, the aim of this study was to verify the contribution of acid growth in the soybean embryonic axis germination. Acid growth is mediated by the acidification of extracellular medium due to the action of H+-ATPases, which activate expansins. We found that the expression of expansins was significantly increased throughout the germination. The expression of H+-ATPases was significantly increased at 3 and 24 h after imbibition (HAI), with major pumping activity at 24 HAI. The auxin and ABA signaling cascades during soybean germination suggest that these hormones are involved with the regulation of H+-ATPase in germinating soybean. To verify the influence of auxin and ABA on H+-ATPase functioning during germination, we treated seeds with IAA, 2,4D, ABA, and ATPase inhibitor, and germinated them in purple agar medium. We observed that IAA, 2,4D, and ABA affected H+-ATPase functioning, by delaying or inhibiting soybean germination. Our results indicate the role of acid growth controlled by H+-ATPase and its regulators—auxin and ABA—in the soybean embryonic axis during germination.

Seeds doi: 10.3390/seeds4030042

Authors: Polina Kuryntseva Darya Tarasova Nataliya Pronovich Ilsina Gilmutdinova Gulnaz Galieva Liliya Biktasheva Svetlana Selivanovskaya

Increasing demand for high-quality food is driving the development of biologized farming methods, which involve the use of microorganisms, including endophytes, to stimulate plant growth. However, research on the composition of endosphere microbiomes is limited. The study presents an analysis of the bacterial endophytic microbiome in lettuce seeds (Lactuca sativa L., cv. Ozornik) using high-throughput sequencing of 16S rRNA amplicons. It evaluates the taxonomic composition and putative functional properties of seed endophytic bacteria. The microbial community exhibited low diversity (Shannon index ranged from 1.1 to 1.84, Simpson index from 0.57 to 0.83). The bacterial endophytic community of lettuce seeds was dominated by Pseudomonadota (83%), Actinomycetota (14%), and Bacillota (3%). The genera identified within the microbiome included Pantoea (32%), Rhodococcus (13%), Candidatus Profftella (13%), Janthinobacterium (7%), Pseudomonas (9%), Enterococcus (3%), and Alcaligenes (2%), which exhibit a broad spectrum of beneficial properties: plant growth promotion (PGPB), suppression of phytopathogens, enhanced stress tolerance, participation in contaminant biodegradation, and heavy metal detoxification. The structure and functional potential of the microbiome vary between samples, potentially due to differences in source material and cultivation conditions. The obtained results expand our understanding of the composition and functions of endophytic bacteria in lettuce seeds, which is important for the development of novel biocontrol agents for plants consumed by humans in an unprocessed form.

Seeds doi: 10.3390/seeds4030041

Authors: Ander Castander-Olarieta Javier Herrero Caroline Teyssier Paloma Moncaleán Itziar A. Montalbán

Because of the effects of climate change, a shortage of seeds from superior mother trees for forestry breeding programmes can occur. While biotechnological tools can help address this challenge, the benefits and drawbacks of each technique need to be evaluated. In this work, a comparative Pinus radiata plantlet production study was performed using two different approaches: seeds versus somatic embryos. For each procedure, the number of plantlets obtained was recorded, and the manual labour time was calculated. The skills and time required were higher for somatic embryogenesis, but so was the number of plantlets obtained: 3660 acclimatized somatic plantlets versus 1124 acclimatized zygotic plantlets. The number of different genotypes obtained was higher when germinating mature seeds, but somatic embryogenesis coupled with cryopreservation offers the advantage of having a backup for field-tested material. Additionally, analyses of carbohydrates, total protein and dry weight of somatic and zygotic embryos were carried out. These analyses revealed considerable differences between somatic and zygotic embryos but underlined the importance of high sucrose levels for germination in both systems. In conclusion, each technique offers advantages and disadvantages, and the choice will depend on the objective, the species and the value of the material to be propagated.

Seeds doi: 10.3390/seeds4030040

Authors: Muhammad Shoaib Tufail Gaye L. Krebs Muhammad S. Quddus Alison Southwell John W. Piltz Mark R. Norton Peter C. Wynn

Food and nutritional insecurity, alongside poverty, remain formidable challenges within smallholder crop–livestock mixed farming systems, predominantly found in Asia and Africa, which are the primary focus of this review. Livestock stands as a crucial asset in these systems, providing food and income for families. However, livestock productivity is often constrained by poor-quality feed, predominantly composed of crop residues. This is compounded by limited access to high-quality forage seeds and the misconception that limited land and water resources should be devoted to cereal production. Furthermore, formal seed supply chains for forages are often underdeveloped or non-existent, making it difficult for farmers to access quality seed. The integration of high-quality legume forages into these systems offers a cost-effective and sustainable solution for improving livestock productivity. These forages provide more nutritious feed and enhance soil fertility through nitrogen fixation, helping to reduce farmers’ reliance on expensive commercial feeds and fertilizers. Success in the adoption of improved forage varieties hinges on participatory approaches that actively engage farmers in varietal selection and evaluation. Such collaboration leads to better adoption rates and increases on-farm productivity, facilitating the establishment of village-based forage seed enterprises (VBFSEs). These enterprises offer a reliable local seed supply of quality seeds, reducing farmers’ dependency on inconsistent national and international seed suppliers. These initiatives not only improve the production of high-quality forage and livestock productivity but also create opportunities for income diversification, contributing to the livelihoods of smallholder farmers. By fostering collaboration and sustainable practices, policymakers and stakeholders, particularly farmers, can build more resilient agricultural systems that support food security and poverty alleviation in rural communities.

Seeds doi: 10.3390/seeds4030039

Authors: Assunta Bertaccini Reena Reddy Gandra Sritej Mateeti Francesco Pacini

Recent research has demonstrated a presence inside the seeds of several plant species of endophytic bacteria that can directly or indirectly interact with germination and seedling growth. Phytoplasmas are plant-pathogenic bacteria that severely impact the agricultural productivity of several crops, including alfalfa, a crucial forage crop in which seed transmission was reported. Therefore, understanding the transmission pathways of phytoplasmas is essential for developing effective control strategies. This study investigates the seed transmission of phytoplasmas in alfalfa using seeds collected in Oman in 2002 and kept in a dry environment in a laboratory for 20 years. The sterilized seeds were germinated and grown in agar medium under sterile conditions and transplanted in soil under greenhouse-controlled insect-proof conditions. Utilizing polymerase chain reaction (PCR) and nested PCR followed by RFLP and sequencing analyses, the alfalfa seedlings were screened for the phytoplasma presence. The detection of phytoplasmas in 16SrIII, 16SrV, 16SrX, and 16SrXII groups was achieved, confirming the preliminary results obtained in the 2002 testing of the same seed batches. This finding indicates that seed transmission could be a critical pathway for the spread of these pathogens in alfalfa, considering their survival in seeds for more than 20 years. Further investigations into the mechanisms of seed transmission and the development of resistant alfalfa varieties are essential to enhance the sustainability and productivity of alfalfa cultivation, thereby supporting the agricultural sector’s efforts to meet the growing demand for high-quality forages.

Seeds doi: 10.3390/seeds4030038

Authors: Adriana Slavova-Kazakova Marina Marcheva Sabina Taneva Svetlana Momchilova

Camelina (Camelina sativa L.) is a valuable source of seed oil, which contains above 30% of essential omega-3 fatty acid (alpha-linolenic acid, 18:3). On the one hand, this high content of linolenic acid is healthy and hence preferable, but on the other hand, highly unsaturated oils are easily deteriorated. The stabilization of such oils with respect to their oxidative changes is a significant problem in oil technology and is directly related to the quality of food, pharmaceutical and cosmetic products. Therefore, the aim of this work was to evaluate the effect of ascorbyl palmitate in various concentrations (0.1–2.0 mM) as an additional antioxidant in camelina seed oil during its autoxidation, by determination of oil induction periods and initial rates of oxidation. The results revealed that the added ascorbyl palmitate caused a decrease in oxidation rate, but in terms of oil stability, opposite effects were observed depending on its concentration. Thus, at low levels (0.1–0.2 mM), ascorbyl palmitate had a pro-oxidant effect and the induction period decreased; no effect was observed in its presence of 1.0 mM, whereas 2.0 mM ensured a much higher protective effect. Rosmarinic acid as an individual antioxidant (at 0.2 mM) increased the stability of camelina oil, while in a mixture (1:1) with ascorbyl palmitate at the same concentration, its effect/activity was significantly reduced.

Seeds doi: 10.3390/seeds4030037

Authors: Arnaud Busquère Dominique Lefebvre Patrice Galaup Lucas Tricoulet Charline Musset Eric Lacroux Othmane Merah

Amaranth is considered a gluten-free, protein-rich pseudocereal. However, seed dormancy affects the germination rate and production. The aim of this study was to investigate the effects of osmopriming, hydropriming, priming with Algal and chia-seed extract biostimulants, scarification, and different combinations of them on seed germination. The results showed that hydropriming, osmopriming, (at least by 13%), and speed (two days earlier) of germination compared to the control. The same trend was observed, to a lesser extent, for priming with a biostimulant. The production of osmopriming has recently started in France. However, seed priming offers a promising solution to overcome the lack of germination. The aim of this study was to test several priming methods and their impact on amaranth seed germination. An imbibition curve was first established and showed that the first two germination phases were reached at 12 h after imbibition. Duration had no effect on germination compared to the control. In contrast, increasing the rate measurement of polyphenol oxidase (PPO) activity revealed a peak during the first few hours. The largest peak was observed for this. These results suggest growing amaranth by increasing the germination rate.

Seeds doi: 10.3390/seeds4030036

Authors: Carla Sousa Carla Guimarães Moutinho Márcia Carvalho Carla Matos Ana Ferreira Vinha

Seeds of Cucurbitaceae crops represent a promising yet underexplored source of bioactive compounds with potential applications beyond nutrition, particularly in the cosmetics industry. This review examines the seeds of Citrullus lanatus (watermelon), Cucumis melo (melon), and Cucurbita pepo (pumpkin), focusing on their biochemical composition and evaluating their functional value in natural cosmetic development. Although these fruits are widely consumed, industrial processing generates substantial seed by-products that are often discarded. These seeds are rich in polyunsaturated fatty acids, proteins, carbohydrates, and phytochemicals, positioning them as sustainable raw materials for value-added applications. The incorporation of seed-derived extracts into cosmetic formulations offers multiple skin and hair benefits, including antioxidant activity, hydration, and support in managing conditions such as hyperpigmentation, acne, and psoriasis. They also contribute to hair care by improving oil balance, reducing frizz, and enhancing strand nourishment. However, challenges such as environmental instability and low dermal permeability of seed oils have prompted interest in nanoencapsulation technologies to improve delivery, stability, and efficacy. This review summarizes current scientific findings and highlights the potential of Cucurbitaceae seeds as innovative and sustainable ingredients for cosmetic and personal care applications.

Seeds doi: 10.3390/seeds4030035

Authors: Igor F. Turkanov Elena V. Bondarchuk Valery G. Gryaznov Ekaterina A. Galkina Alexey Yu. Guzenko Vladimir G. Zainullin Elena G. Kozar Irina M. Kaigorodova

Numerous scientific studies have confirmed the effectiveness of seed bioactivation using electromagnetic fields (EMFs) in agriculture. This article presents the results of the remote application of an EMF TOR device in the cultivation of barley Hordeum vulgare L. Laboratory studies and field tests were conducted, showing a positive effect on the growth and development of plants both when treating dry seeds before sowing and when treating sown seeds in the field. The optimal time period for EMF treatment was determined: treating air-dried seeds with EMFs before sowing for 10–15 min increased germination by 5–18% and the growth rate of seedlings by 2–3 times. The maximum observed effect occurred during the treatment period from 7:00 to 11:00. As a result of changing the balance of phytohormones, the further stimulation of the root system and the assimilation surface of plants was noted due to a 1.5-fold increase in the content of auxins. The density of productive stems, ear length, seed set, and 1000 seed weight increased, which ultimately led to an increase in yield by more than 10% and, in some varieties, to a decrease in the protein content in grains compared to the control variant (by 3–22%), bringing them closer to brewing conditions.

Seeds doi: 10.3390/seeds4030034

Authors: Javier Giovanni Álvarez-Herrera Julián Stiven Lozano Oscar Humberto Alvarado-Sanabria

Peas possess significant nutritional properties due to their high protein levels, carbohydrates, fiber, and vitamins. Increased climate variability can lead to water stress in crops like peas. Therefore, priming plants through seed priming is a technique that has proven effective as a pre-conditioning method for plants to cope with more severe future stresses. Different doses and soaking times of ‘Santa Isabel’ pea seeds in NaCl and H2O2 were evaluated to enhance and promote germination. Two experiments were conducted under controlled conditions (average temperature 15.8 °C) through a completely randomized design with a 4 × 3 factorial arrangement, comprising 12 treatments in each trial. In the first trial, NaCl doses (0, 50, 100, or 150 mM) and the soaking time of the seeds in NaCl (12, 24, or 36 h) were examined. In the second trial, H2O2 doses (0, 20, 40, or 60 mM) were tested with the same imbibition times. The 50 mM NaCl dose at 24 h demonstrated the best values for germination rate index, mean germination time, germination rate (GR), and germination potential (GP). Seed imbibition for 24 h in NaCl, as well as in H2O2, is the ideal time to achieve the best GR and GP. The dry mass of leaf and stipule recorded the highest values with a 60 mM dose of H2O2 and 24 h of imbibition. An application of 150 mM NaCl resulted in the highest values of germinated seed dry mass, while causing lower dry mass in roots, stems, leaves, and stipules; however, it maintained similar total dry mass values.

Seeds doi: 10.3390/seeds4030033

Authors: João Henrique Constantino Sales Silva Joyce Naiara da Silva Luís Gustavo Alves de Almeida Eduardo Luã Fernandes da Silva Aline das Graças Souza Edna Ursulino Alves

Guilandina bonduc L. is a pantropical coastal shrub with varied fruits and seeds, capable of germinating under saline stress. This study aimed to morphologically characterize the fruits and seeds of the species, correlate these characteristics, and evaluate the tolerance of seedlings to salt according to seed mass. Physical variables (length, width, thickness, and weight) were analyzed, and Spearman’s correlation was applied. Germination was tested with light seeds (<1.55 g) and heavy seeds (≥1.55 g) under five levels of salt stress, in a 2 × 5 factorial design. G. bonduc can produce seeds with variations in mass and size that are not necessarily related to fruit size. The reduction in osmotic potential resulted in lower seed germination and vigor; even so, the species demonstrated tolerance to salt stress, maintaining germination rates above 50% even under conditions of −1.0 MPa, regardless of seed mass. Lighter seeds germinate more quickly and uniformly, while heavier seeds produce more vigorous seedlings, especially in the absence of salinity, and are therefore more suitable for seedling production. These results indicate that G. bonduc has potential for revegetation of saline areas, being useful in adaptation to climate change due to its tolerance to saline stress and the relationship between seed mass and seedling vigor.

Seeds doi: 10.3390/seeds4030032

Authors: Birtukan Tolera Geleta Jae-Yun Heo

Aegopodium podagraria (A. podagraria) L. is a perennial herb valued for its medicinal properties but exhibits poor germination and inconsistent growth under conventional cultivation. To overcome these limitations and enhance its functional potential, this study investigated the effects of various LED light spectra on the plant’s physiological and antioxidant responses under controlled indoor conditions. Six light treatments were applied, consisting of different red (R) and blue (B) light ratios (R100, R80:B20, R60:B40, R40:B60, and B100), along with a white-light control. Red-dominant treatments, particularly R80:B20, not only improved germination traits but also significantly promoted shoot growth and biomass accumulation. In contrast, higher proportions of blue light generally inhibited germination performance and reduced growth-related parameters compared to the white-light control. Antioxidant activity was also modulated by light quality: R80:B20 induced the highest levels of total phenolics, ferric reducing antioxidant power, and vitamin C, whereas R40:B60 maximized flavonoid content and DPPH radical scavenging activity. These results suggest that optimizing the red-to-blue light ratio can effectively enhance both the cultivation performance and biofunctional quality of A. podagraria in controlled environments.

Seeds doi: 10.3390/seeds4030031

Authors: Aikaterini N. Martini Maria Papafotiou

With the aim of developing an efficient propagation method for the exploitation of Thymelaea hirsuta and T. artonraira ssp. tartonraira in the xeriscaping and pharmaceutical industry, the effects of the following were examined on the in vitro germination of their seeds: (i) pretreatment (mechanical and chemical scarification or immersion in hot water; (ii) incubation temperature (5–30 °C); (iii) incubation light conditions (16 h photoperiod or continuous darkness); (iv) storage period at room temperature and darkness (up to 24 months). Seeds collected for two years from the same wild plants in Greece were surface-sterilized with a 15% commercial bleach solution for 15 min after the abovementioned treatments and placed for germination in Petri dishes containing a half-strength MS medium in growth chambers. The rate and final percentage of germination were recorded. For both species, scarification after immersion in concentrated H2SO4, preferably for 20 min, was necessary for seed germination, which indicates coat dormancy. Higher germination percentages were observed at temperatures of 10–20 °C, under continuous darkness for T. hirsuta (79–100%) and regardless of photoperiod for T. tartonraira (73–90%). Long storage reduced germination of only T. tartonraira (54–68% at optimum temperatures, 23 months after harvest), while T. hirsuta seeds stored for 5 months germinated at significantly lower percentages (40% maximum) compared to seeds stored for 9–24 months, revealing a dry after-ripening process. Seeds of both species harvested at different years showed stable behavior in terms of germination. For both species, an effective seed propagation protocol suitable for their exploitation as ornamental and landscape plants was developed.

Seeds doi: 10.3390/seeds4030030

Authors: Beibei Wu Haoran Liang Jiahan Lv Rui Liu Nenghui Ye

Seed germination is the initial step in a plant’s life cycle; it is precisely regulated by many factors at the molecular and biological levels. Reversible protein phosphorylation, which is regulated by protein kinases and protein phosphatases, plays a key role in hormone signal transduction, energy metabolism, stress response, and plant growth and development, including seed germination. This review provides a comprehensive elucidation of the coordinated regulatory mechanisms mediated by kinases and phosphatases during seed germination, with particular emphasis on their dynamic interplay and reciprocal modulation within biological signaling networks. Through the systematic integration of current research findings, we mechanistically dissect the sophisticated phosphorylation–dephosphorylation circuitry that governs metabolic activation, hormonal signaling transduction, and cellular homeostasis in germinating seeds. Furthermore, we propose a novel conceptual framework that delineates the spatiotemporal cooperation between these opposing enzymatic activities in regulating dormancy release and developmental transitions. The current challenges in the field of seed germination research are critically examined, and potential future investigative trajectories are outlined, aiming to establish a robust theoretical framework for elucidating the molecular mechanisms underlying seed dormancy regulation, as well as translating these findings into innovative agricultural production practices.

Seeds doi: 10.3390/seeds4030029

Authors: Mohammad Saidur Rhaman

Seed germination, a pivotal stage in the plant life cycle, profoundly impacts crop growth and establishment. However, fluctuating environmental conditions like drought, salinity, severe temperatures, and heavy metal toxicity impede seed germination rates and seedling vigor. Seed priming is a pre-sowing seed treatment that involves the controlled hydration of seeds, proven to improve germination rate and stress resilience. It initiates pre-germinative metabolism, including enzyme activity, antioxidant accumulation, hormone modulation, and cellular repair, without radicle emergence. Recent advancements in seed priming, encompassing the application of nanoparticles, phytohormones, and beneficial microbes, have significantly broadened its potential. Despite its proven benefits, challenges such as reduced seed longevity post-priming and variability in species-specific responses remain. This paper revisits the principles and methodologies of seed priming, highlighting its physiological, biochemical, and molecular mechanisms that enhance germination under stress conditions. Additionally, it addresses current challenges and future research directions for optimizing seed priming as a low-cost, eco-friendly approach to improve crop establishment under adverse environments, thereby supporting resilient and sustainable agriculture.

Seeds doi: 10.3390/seeds4030028

Authors: Allison A. Mertin Linda L. Blackall Douglas R. Brumley Edward C. Y. Liew Marlien M. van der Merwe

Seed microbes play crucial roles in plant health, but studying their diversity is challenging due to host DNA contamination. This study aimed to optimise methodologies for investigating seed microbiomes across diverse plant species, focusing on the efficacy of peptide nucleic acid (PNA) clamps to reduce host DNA amplification. We tested PNA clamps on three plant species: Melaleuca quinquenervia (tree), Microlaena stipoides, and Themeda triandra (grasses). The effectiveness of PNA clamps was assessed through in silico analysis, axenic tissue culture, and metabarcoding techniques. In silico analysis confirmed the specificity of PNA clamps to the 16S rRNA gene V4 region of chloroplasts in the grass species. Axenic tissue culture experiments showed that applying PNA clamps at both 1 µM and 0.25 µM concentrations significantly reduced plant DNA amplification. Metabarcoding analyses further confirmed that PNA clamps effectively suppressed host DNA, enhancing microbial diversity estimates across all three species while preserving core microbial taxa. The efficacy of the clamps varied among host species, with T. triandra exhibiting the highest blocking efficacy, and chloroplast clamps outperforming mitochondrial ones. This study demonstrates that PNA clamps are a useful for improving seed endophyte metabarcoding datasets, although they require optimisation for some plant species. This knowledge will contribute to enhancing our understanding of seed microbiome diversity and its ecological implications.

Seeds doi: 10.3390/seeds4020027

Authors: Stefania Mazzini Marco Zuccolo Angela Bassoli Claudio Gardana Gigliola Borgonovo

Solid-state 13C NMR spectroscopy using cross-polarization magic-angle spinning is a highly valuable technique for the semi-quantitative analysis of complex solid matrices. One of its key advantages is that it does not require any manipulation of the matrix, such as extractions or other treatments, which is particularly important for preserving the integrity of unstable secondary metabolites. Glucosinolates (β-thioglucoside-N-hydrosulphates) are crucial secondary metabolites specific to Brassica species, and many of them are known to be highly unstable. In this study, we evaluated solid-state nuclear magnetic resonance spectroscopy as an alternative method for the identification and quantification of total glucosinolates in the seeds of Sisymbrium officinale, Brassica napus, Sinapis alba, Brassica nigra, and Moringa oleifera. The results obtained with this method showed good agreement with those from conventional chemical analyses of the seed material. Although, based on a limited number of samples, this preliminary study suggests that the proposed approach could be a useful alternative for quantifying total glucosinolate content in seeds.

Seeds doi: 10.3390/seeds4020026

Authors: Liuqin Zhang Mingxuan Xu Qingqing Li Lei Hou Mi Zhang

Seed germination is a crucial phase where a plant embryo transitions from dormancy to active growth, emerging as a seedling. This intricate process is highly susceptible to environmental cues, particularly abiotic stress factors including drought, salinity, and temperature extremes, which can profoundly influence both germination success and subsequent plant development. Among the various cellular components that modulate plant responses to these stresses, Rho of Plants (ROP) emerges as a pivotal regulator. Under abiotic stress, ROP signaling components integrate with the core abscisic acid (ABA) signaling pathway by regulating gene transcription and protein stability, modulating subcellular localization, converting protein activity, and engaging in competitive interactions. This review summarizes recent findings on roles of ROP signaling in regulating plant adaptive responses to abiotic stress, whilst explores potential involvement of ROPs in seed germination. This review summarizes the effects of ROP proteins and their effectors, such as GEF, on the seed germination process. It preliminarily elucidates the crosstalk mechanisms between these proteins and the ABA signaling pathway, thereby gaining a deeper understanding of the role of ROP signaling in regulating plant adaptive responses to abiotic stresses.

Seeds doi: 10.3390/seeds4020025

Authors: Shraddha Shridhar Gaonkar Lorenzo Ciceri Matteo Romelli Andrea Pagano Alessio Giovannelli Pier Mario Chiarabaglio Alma Balestrazzi Anca Macovei

Populus alba clone ‘Villafranca’ (white poplar), highly suitable for biomass production and ecosystem restoration, is a model system for molecular and physiological studies, but no reports are available concerning seed quality. Although clonal propagation is the preferred approach for commercial purposes, attention should be given to face genetic variability losses in the existing germplasm. To address this challenge, new populations should be developed starting from seeds, overcoming the issues of low germinability and viability during storage. This study proposes to develop tailored treatments to improve the germination of long-term stored white poplar seeds. Priming and soaking protocols, based on the use of water or spermidine (Spd, 50 and 100 μM), were tested. Treatment efficacy was assessed based on germination parameters, reactive oxygen species (ROS) profiles, and the expression patterns of genes with key roles in early seed germination. Soaking with 100 μM Spd for 4 h significantly enhanced germination percentage and speed. Low ROS levels were evidenced in the Spd-treated seeds, compared to water-soaked seeds. High expression of genes involved in desiccation tolerance acquisition, polyamine biosynthesis, and antioxidant defense was observed only in dry seeds. The results are discussed in view of the potential protective role of Spd.

Seeds doi: 10.3390/seeds4020024

Authors: Piao Yang Ling Lu Abraham Condrich Gavin A. Muni Sean Scranton Shixiang Xu Ye Xia Shuai Huang

Seed science is the comprehensive study of seeds. It encompasses their biology, production, technology, genetics, physiology, ecology, and applications in agriculture and conservation. Seed science has undergone transformative advancements through the integration of microbial technologies, with beneficial microorganisms emerging as critical tools for enhancing germination, seedling vigor, and crop resilience. Research demonstrates that microbial treatments improve nutrient uptake, hormonal regulation, and stress tolerance while establishing early symbiotic relationships with plants. This review synthesizes recent advances in understanding the roles of beneficial microbes in seed science, focusing on their impact on seed germination, seedling growth, and plant health. We explore the composition and transmission of seed microbiomes, highlighting the vertical transfer of microbes from parent plants to seeds and the influence of environmental factors on microbial community structure. The review also discusses innovative approaches to seed microbiome engineering. Particular attention is given to seed biopriming with plant growth-promoting bacteria (PGPB), which has shown significant potential in improving germination rates, seedling vigor, and crop productivity. Specific microbial strains, such as Trichoderma species and Pseudomonas fluorescens, are discussed with emphasis on their mechanisms of action in enhancing plant performance. The review also addresses the impact of breeding on seed microbiomes and explores emerging research directions, including the development of tailored microbial inoculants and the investigation of intracellular seed bacteria. By synthesizing these findings, this review aims to provide a comprehensive summary of the current state of seed microbiome research and its implications in seed science for sustainable agriculture.

Seeds doi: 10.3390/seeds4020023

Authors: Grace Carapezza Sandra B. Wilson Mica McMillan Edzard van Santen

Seed propagation is the primary means of reproducing many native and endemic species, including garberia [Garberia heterophylla (W. Bartram) Merrill & F. Harper]. This attractive pollinator plant, native to Florida, is scarcely found in nursery production and largely unknown to the gardening community. To better understand the seed biology of garberia, a series of experiments were conducted to evaluate the effects of population on seed viability and germination response to four seasonal temperatures, as well as the effects of time on seed storability. Initial seed viability was 49% and 60% for Central and North Florida populations, respectively. Seeds germinated readily, indicating non-dormancy, with significant effects of population and temperature observed. Overall, on day 28, a greater germination proportion was observed from seeds collected from North Florida than Central Florida across temperatures, except for winter (11/22 °C), where responses were similar. The greatest germination proportion for seeds collected from North Florida was observed at 15/27 °C (fall) and 19/29 °C (spring), whereas the greatest germination from Central Florida was observed at 11/22 °C (winter), with the steepest decline observed at summer temperatures (24/33 °C). Further, it was observed that garberia seeds are intolerant of long-term storage, losing viability as early as 3 months under conventional cold or room temperature storage and decreasing substantially more after 6 months. These findings contribute to the overall understanding of the seed biology of underutilized species such as garberia, key to the development of efficient and reliable propagation systems for our nursery industry.

Seeds doi: 10.3390/seeds4020022

Authors: Ali A. Alzandi Ibrahim M. Aref Nels Grevstad

Acacia species are important trees in arid ecosystems due to their diverse ecological roles, such as providing vegetation cover, community structures, food resources for animals, soil stabilization, and erosion prevention. However, in the Arabian Peninsula, Acacia species are declining due to climate change, overgrazing, and fuelwood harvesting. This study evaluates the effectiveness of various pre-sowing treatments—sulfuric acid soaking and tap and hot water soaking—on breaking seed dormancy to enhance germination in nine Acacia species native to the Al-Baha region of Saudi Arabia. The key germination indicators assessed were the mean germination time (MGT), germination percentage (GP), and germination index (GI). Sulfuric acid treatments for 10–15 min reduced the MGT and increased the GP for A. etbaica, A. hamoulosa, and A. tortilis, while A. origena responded best to 1 min of hot water soaking. Conversely, A. asak, A. ehrenbergiana, and A. johnwoodii showed little to no germination improvement with treatment and A. oerfota and A. gerrardii showed no germination improvement, indicating the need for alternative methods. These findings indicate that the seed germination requirements vary within Acacia spp. from the same geographic region and similar climatic conditions. Further work is required for five of the species tested to develop better seed germination techniques, given the potential utility of Acacia spp., in ecological restoration and sustainable land management in arid regions.

Seeds doi: 10.3390/seeds4020021

Authors: Alyssa D. Babb Mirwais M. Qaderi

Crops are continually subjected to frequent and extreme changes in climate, such as high temperatures and soil water deficits. Many studies have shown the individual effects of these factors on plants, but their combined effects on reproductive growth and subsequent seed germinability have received little attention. In this study, we used canola (Brassica napus) plants and grew them through their lifecycle under two temperature regimes (20/10 °C and 24/14 °C, 16 h light/8 h dark) in controlled-environment growth chambers. Half of the plants were watered to field capacity (well-watered) and the other half at wilting point (water-stressed). During the reproductive stage, the flower, silique, and seed traits were measured. Higher temperatures decreased the petal width by 1.17 times but increased petal anthocyanins by 1.03 times. The water deficit decreased the silique length and total seed number by 1.21 and 1.32 times, respectively, but increased nectar sugar concentration by 1.28 times. The total volume of nectar was affected by the interaction of temperature and water. The nectar volume was lowest in the water-stressed plants under higher temperatures (2.66 ± 0.29 µL per flower) but highest in the well-watered plants under the same temperature regime (5.73 ± 0.37 µL per flower). In conclusion, the combined effects of temperature and water were less pronounced than the individual effects of these factors on canola reproductive yield.

Seeds doi: 10.3390/seeds4020020

Authors: Valentina Rastelli Valeria Giovannelli Giovanni Staiano Pietro Massimiliano Bianco Alfonso Sergio Matteo Lener

This paper presents a pilot project conducted by ISPRA and ARPA Campania to develop a monitoring protocol to detect the presence of genetically modified (GM) oilseed rape (Brassica napus) plants resulting from accidental seed dispersal during transportation from entry points to storage and processing facilities; the project has been implemented in Italy’s Campania region. The unintentional dispersal of GM oilseed rape seeds and the potential establishment of feral populations have been identified as environmental concerns in various countries, even when GM oilseed rape is imported solely for processing and not for cultivation. The project activities were designed, taking into account the characteristics of the Italian environment and infrastructures. Multiple sampling campaigns were conducted in autumn 2018, spring 2019, and autumn 2019 to validate the selected transects and assess the presence of Brassicaceae species, with a particular focus on oilseed rape. These efforts involved direct monitoring and sample collection along transport routes from the port of Salerno to seed companies in the provinces of Benevento and Caserta. Field observations and import data revealed a decrease in oilseed rape movement at the port of Salerno in the years preceding the survey, while seed companies near Benevento remained active sites for white mustard (Sinapis alba). The presence of S. alba and the simultaneous occurrence of oilseed rape and Raphanus raphanistrum—a species with high hybridization potential—support the hypothesis that seed companies may act as hotspots for accidental seed dispersal and that potential interspecific gene flow can occur. The study also validated the adopted sampling and molecular analysis methods, including DNA extraction and PCR, for the detection of the Cruciferin A (CruA) gene in all Brassica species collected. These findings highlight the need to strengthen post-marketing monitoring plans, even when GM rapeseed is imported solely for processing, to mitigate the potential risks associated with unintended gene flow.

Seeds doi: 10.3390/seeds4020019

Authors: Diego Rivera Manuel Martínez-Rico Jacob Morales Francisco Alcaraz Javier Valera Dennis Johnson Pedro A. Sosa Javier Abellán Jose Antonio Palazón Diego José Rivera-Obón Emilio Laguna Concepción Obón

The taxonomic complexity of Phoenix palms in the Canary Islands, where multiple morphotypes representing at least four taxa currently exist, presents significant challenges for archaeobotanical identification. We developed a Bayesian probabilistic framework to identify archaeological Phoenix seeds within the context of genus-wide morphological diversity. Our analysis incorporated thousands of specimens including modern reference collections, archaeological materials from pre-Hispanic sites in Gran Canaria and La Gomera (3–16th centuries CE), and fossil remains. We recorded quantitative measurements and qualitative characteristics for each specimen. To understand taphonomic effects, we conducted experimental carbonization of modern P. canariensis seeds and documented the resulting morphological alterations. We performed a hierarchical cluster analysis using Ward’s minimum variance method and calculated taxonomic assignment probabilities for archaeological specimens using Bayesian inference, where likelihood was derived from taxon proportions within assigned clusters. The results indicated a high probability (0.69–1.00) that the archaeological specimens belong to P. canariensis var. canariensis, with no evidence for P. dactylifera presence. These findings provide critical insights into pre-Hispanic exploitation of Phoenix palms, particularly the endemic P. canariensis, which served as a vital resource, providing food, fiber, and construction materials. Our methodological approach offers a robust framework for addressing taxonomic uncertainty in archaeobotanical research while enhancing understanding of historical palm biogeography and resource use patterns in the Canary Islands.

Seeds doi: 10.3390/seeds4020018

Authors: Suzanne P. Boschma Hugh W. McDouall Jonathan W. McLachlan

Annual grass weeds can provide significant competition to an establishing sown tropical perennial grass pasture. At least two years of grass weed control prior to sowing is required to reduce the weed seed bank. Pre-emergent herbicides used in summer cereals, such as atrazine or s-metolachlor with metcamifen seed safener, may reduce this preparation time. Two controlled-environment experiments were conducted to assess the potential for these pre-emergent herbicides to be used with several tropical perennial grasses. Experiment 1 tested the effect of metcamifen (400 g L−1 a.i. at 0–2× label rate) on the emergence and vigor of Chloris gayana, Dichanthium aristatum, Digitaria eriantha and Panicum coloratum, with Sorghum bicolor as the control. Experiment 2 tested the effect of s-metolachlor (960 g ha−1 a.i.) with metcamifen-treated or untreated seed, and atrazine (1800 g ha−1 a.i.) on the emergence and early growth of the grasses. Metcamifen did not inhibit emergence or vigor of the grasses. Without metcamifen seed treatment, s-metolachlor reduced the growth of the tropical perennial grasses by 47–100%, while it had no such effect on S. bicolor. In contrast, there was no effect of atrazine on shoot yields of the grasses, nor of s-metolachlor when D. aristatum, D. eriantha and P. coloratum seed had been treated with metcamifen. The collective results indicate that the herbicide safener metcamifen does not reduce the viability of tropical perennial grass seed and provides some protection against s-metolachlor, albeit not complete protection at the rates used in our study. Atrazine did not affect emergence or early growth of the grasses.