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20 pages, 3955 KiB  
Article
Ion Currents Mediated by TRPA1 Channels in Freshly Dissociated Rat Articular Chondrocytes: Biophysical Properties and Regulation by Inflammatory Processes
by Arturo Ponce, Lidia Jimenez, Maria Luisa Roldan and Liora Shoshani
Pharmaceuticals 2025, 18(3), 332; https://doi.org/10.3390/ph18030332 - 26 Feb 2025
Viewed by 1
Abstract
Background: Articular chondrocytes are specialized cells in synovial joint cartilage, responsible for maintaining and regenerating the extracellular matrix. Inflammation disrupts the balance between matrix synthesis and degradation, leading to cartilage breakdown. This process, commonly observed in conditions such as osteoarthritis, results in [...] Read more.
Background: Articular chondrocytes are specialized cells in synovial joint cartilage, responsible for maintaining and regenerating the extracellular matrix. Inflammation disrupts the balance between matrix synthesis and degradation, leading to cartilage breakdown. This process, commonly observed in conditions such as osteoarthritis, results in chondrocyte dysfunction and accelerates joint degeneration. Since TRPA1 channels are implicated in inflammatory processes, this study investigates the expression of TRPA1 channels in freshly dissociated rat articular chondrocytes and their modulation by anti-inflammatory agents. Methods: We used the whole-cell patch-clamp method to assess TRPA1 channel expression and modulation. Results: Freshly dissociated chondrocytes exhibit ion currents attributable to TRPA1 channel expression, with higher magnitudes observed in medium-sized cells. These currents decrease over time in primary culture. Treatment with pro-inflammatory agents (IL-1α, IL-1β, and LPS) increases TRPA1′s current magnitude. IL-1β treatment directly induces transient TRPA1 currents. Several signaling components activated during inflammation contribute to the IL-1β-induced enhancement of TRPA1 current density, including IL-1 R1, the adaptor protein MyD88, and the downstream kinases IRAK1 and IRAK4. Conclusions: Our findings demonstrate that healthy rat chondrocytes express functional TRPA1 channels and that inflammatory processes modulate their expression. Full article
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13 pages, 3969 KiB  
Article
Transcriptomic and Lipidomic Characteristics of Subcutaneous Fat Deposition in Small-Sized Meat Ducks
by Hao Zheng, Cui Wang, Ao Zhou and Xing Chen
Metabolites 2025, 15(3), 158; https://doi.org/10.3390/metabo15030158 - 26 Feb 2025
Viewed by 76
Abstract
Background: Subcutaneous fat deposition is associated with ducks’ meat quality and the methods used to cook them. However, the reasons underlying the differences in the lipid deposition of small-sized Wuqin10 meat ducks remain unclear. Method: In the present study, to elucidate the metabolic [...] Read more.
Background: Subcutaneous fat deposition is associated with ducks’ meat quality and the methods used to cook them. However, the reasons underlying the differences in the lipid deposition of small-sized Wuqin10 meat ducks remain unclear. Method: In the present study, to elucidate the metabolic mechanisms of lipid deposition, we comprehensively analyzed the transcriptomics and lipidomics of subcutaneous fat in Wuqin10 meat ducks with different subcutaneous thicknesses with six replicates. Results: A total of 1120 lipids were detected in the lipidomic analysis, and 39 lipids were inexorably regulated in the ducks with the thick subcutaneous layer compared to those with the thin layer; further, the up-regulated lipids were primarily triglycerides (TGs), which may have resulted in adipocyte enlargement. Furthermore, the transcriptomic analysis identified 265 differentially expressed genes (DEGs), including 119 down-regulated and 146 up-regulated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses showed that the DEGs were significantly enriched in the histidine, arginine, proline metabolism signaling and adipocytokine signaling pathways. The protein–protein interaction (PPI) network in Cytoscape 3.8.2 identified hub genes HSP90AA1, RUNX2, ACTN2, ACTA1, IL10, CXCR4, EGF, SOCS3 and PTK2, which were associated with the JAK-STAT signaling pathway and regulation of adipocyte hypertrophy. Conclusion: Taken together, our findings reveal the patterns of lipids and the gene expression of subcutaneous fat, providing a basis for future studies of subcutaneous fat deposition in small-sized meat ducks. Full article
(This article belongs to the Special Issue Intestinal Health and Metabolites in Farm Animals)
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15 pages, 1479 KiB  
Article
Obesity-Induced PVAT Dysfunction and Atherosclerosis Development: The Role of GHSR-1a in Increased Macrophage Infiltration and Adipocytokine Secretion
by Sorin Nicolae Peiu, Florin Zugun-Eloae, Bogdan Stoica, Ecaterina Anisie, Diana Gabriela Iosep, Mihai Danciu, Iustina Silivestru-Crețu, Fawzy Akad, Andrei Nicolae Avadanei, Laura Condur, Radu Florin Popa and Veronica Mocanu
J. Cardiovasc. Dev. Dis. 2025, 12(3), 87; https://doi.org/10.3390/jcdd12030087 - 26 Feb 2025
Viewed by 91
Abstract
In obesity, recent research revealed that increased expression of the growth hormone secretagogue receptor (GHSR) in macrophages plays a pivotal role in the development of meta-inflammation, promoting macrophage infiltration and pro-inflammatory polarization. This study aimed to examine the association between GHSR-1a expression in [...] Read more.
In obesity, recent research revealed that increased expression of the growth hormone secretagogue receptor (GHSR) in macrophages plays a pivotal role in the development of meta-inflammation, promoting macrophage infiltration and pro-inflammatory polarization. This study aimed to examine the association between GHSR-1a expression in atherosclerotic plaques and adjacent perivascular adipose tissue (PVAT) from 11 patients with obesity and peripheral artery disease (PAD) who underwent revascularization procedures. Immunohistochemistry was used to assess the expression of CD68, CD80, and CD14, while tissue homogenate levels of adiponectin, leptin, IL-6, and CRP were quantified via ELISA. Serum markers of inflammation were also measured. Among patients with GHSR-1a-positive (+) macrophages in atherosclerotic plaques, we observed significantly higher white blood cell counts and platelet-to-lymphocyte ratios in serum, a lower adiponectin-to-leptin ratio, and elevated IL-6 levels in both arterial and PVAT homogenates. Our findings suggest a link between GHSR-1a and macrophage/monocyte infiltration, macrophage polarization, and adipocytokine secretion in atherosclerotic plaques associated with obesity-induced PVAT dysfunction. Full article
(This article belongs to the Section Basic and Translational Cardiovascular Research)
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15 pages, 2047 KiB  
Article
Unraveling the Extra Virgin Olive Oil Effect on Inflammation and on Gut and Saliva Microbiota
by Marta Correia, Ana T. P. C. Gomes, Inês Moreira, Jane El Maghariki, Karina Mendes, Maria José Correia, Rui Barros, Joana Cristina Barbosa, Nuno Rosa and Ana Maria Gomes
Biomolecules 2025, 15(3), 338; https://doi.org/10.3390/biom15030338 (registering DOI) - 26 Feb 2025
Viewed by 121
Abstract
Extra virgin olive oil (EVOO) with a high content of polyphenols has attracted attention due to its proved beneficial effects in decreasing the risk of cardiovascular disease, modulating cholesterol levels (HDL and LDL), modulating inflammatory markers, and decreasing the levels of haemoglobin1Ac, suggesting [...] Read more.
Extra virgin olive oil (EVOO) with a high content of polyphenols has attracted attention due to its proved beneficial effects in decreasing the risk of cardiovascular disease, modulating cholesterol levels (HDL and LDL), modulating inflammatory markers, and decreasing the levels of haemoglobin1Ac, suggesting that EVOO can have an impact in glycemia regulation. This study assessed the impact of the consumption of a northern Portuguese polyphenol-rich EVOO with a high profile of bioactive molecules on several parameters, such as saliva and serum inflammatory biomarkers, and explored EVOO impact on gut and oral microbiota regarding Bacillota and Bacteroidota content. Thus, the impact on glycated haemoglobin (HbA1c), low-density lipoprotein cholesterol (LDL-C), C-reactive protein (CRP), inflammatory biomarkers, and faecal and salivary microbiomes were evaluated before and after the exposure to EVOO. The results showed that EVOO promotes a decrease in the levels of HbA1C and in the pro-inflammatory interleukin IL-1β, associated with inflammatory processes. Moreover, EVOO intake modulated gut and oral microbiota, increasing Bacteroidota in both ecological niches and Bacillota in the oral microbiota, both phyla being associated with health, demonstrating a prebiotic effect. Full article
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24 pages, 862 KiB  
Article
Changes in Oxidative Stress, Inflammatory Markers, and Lipid Profile After a 6-Week High-Antioxidant-Capacity Dietary Intervention in CVD Patients
by Magdalena Czlapka-Matyasik, Lidia Wadolowska, Paweł Gut and Anna Gramza-Michałowska
Nutrients 2025, 17(5), 806; https://doi.org/10.3390/nu17050806 - 26 Feb 2025
Viewed by 105
Abstract
Background/Objectives: Increased dietary antioxidant capacity is a good means of lowering oxidative stress and cardiovascular risk. Established antioxidant capacity doses should be tested using dietary intervention. Methods: We analysed the influence of a high-antioxidant-capacity diet on oxidative stress (OS) and inflammatory [...] Read more.
Background/Objectives: Increased dietary antioxidant capacity is a good means of lowering oxidative stress and cardiovascular risk. Established antioxidant capacity doses should be tested using dietary intervention. Methods: We analysed the influence of a high-antioxidant-capacity diet on oxidative stress (OS) and inflammatory and lipid profile in CVD (cardiovascular disease) subjects with initially low (LowA) and high (HighA) antioxidant capacity markers. It was an experimental study with a 6-week dietary intervention (DI). Forty-eight CVD patients completed the DI. Blood and urine samples were collected, and anthropometric measurements were taken. Dietary data were collected using a multi-day food record method. α-tocopherol, β-carotene, and retinol were chosen as antioxidant capacity markers; F2-isoprostanes (F2-IsoP), oxidised low-density lipoproteins (oxLDL), and uric acid (UA) were used as OS markers; and interleukin 6 (IL-6) and high-sensitivity C-reactive proteins (hs-CRP) were used as inflammatory markers. Total cholesterol, low- and high-density lipoproteins, and triglycerides (TCHOL, LDL, HDL, TRI) as lipid profiles were analysed. Two groups of subjects with LowA and HighA profiles were identified. Results: The total dietary antioxidant capacity intake during DI was increased by 56%. In the total sample, the DI increased β-carotene, retinol, and UA, and decreased IL-6 oxLDL. The LowA group exhibited increased β-carotene, α-tocopherol, retinol, and decreased IL-6. The HighA group exhibited increased β-carotene and decreased IL-6, F2-IsoP, oxLDL, and oxLDL/LDL ratio. In the HighA group, compared to the LowA group, greater decreases in α-tocopherol and F2-IsoP were found. In both groups, inflammatory markers (IL-6) decreased, and β-carotene increased. Conclusions: The DI results depended on the antioxidant capacity profile at baseline; nevertheless, the established DI including selected antioxidative snacks significantly decrease oxidative stress and improve antioxidant capacity. Further research on diet natural antioxidant supplementation needs to be continued. Full article
(This article belongs to the Section Lipids)
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9 pages, 4227 KiB  
Article
Ionic Liquid Capsules as Flame-Retardant Additives for Emulsion Paint Systems
by Rong Ma, Bingqian Wu and Qingsheng Wang
Polymers 2025, 17(5), 626; https://doi.org/10.3390/polym17050626 - 26 Feb 2025
Viewed by 51
Abstract
To develop a highly efficient and environmentally friendly flame-retardant system, ionic liquids (ILs) have recently emerged as promising candidates. However, the addition of ILs into emulsion paint disrupts emulsion stability, leading to rapid demulsification due to electrostatic effects. Herein, IL–silica capsules were developed [...] Read more.
To develop a highly efficient and environmentally friendly flame-retardant system, ionic liquids (ILs) have recently emerged as promising candidates. However, the addition of ILs into emulsion paint disrupts emulsion stability, leading to rapid demulsification due to electrostatic effects. Herein, IL–silica capsules were developed using a soft-template method. These capsules can be directly added to an acrylic emulsion paint system as flame-retardant additives. Incorporating 5 wt% IL–silica capsules into the system and coating it on fabric reduced flammability by 53% compared to untreated fabric. This work provides a novel and practical approach to enhance flame retardancy in emulsion paint systems without compromising their stability. Full article
(This article belongs to the Section Polymer Chemistry)
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19 pages, 2329 KiB  
Article
Effects of Deoxynivalenol Detoxifier on Growth Performance, Blood Biochemical Indices, and Microbiota Composition of Piglets
by Luyao Zhang, Yongwei Wang, Weiwei Wang, Li Wang, Jingjing Shi, Junlin Cheng, Jing Zhang, Aike Li, Beibei He and Zhiyong Fan
Int. J. Mol. Sci. 2025, 26(5), 2045; https://doi.org/10.3390/ijms26052045 - 26 Feb 2025
Viewed by 64
Abstract
Deoxynivalenol (DON), also known as vomitoxin, has a high detection and exceeding rate in feed and is prone to causing symptoms such as loss of appetite, weight loss, vomiting, and diarrhoea in animals, which brings great harm to the aquaculture industry. The common [...] Read more.
Deoxynivalenol (DON), also known as vomitoxin, has a high detection and exceeding rate in feed and is prone to causing symptoms such as loss of appetite, weight loss, vomiting, and diarrhoea in animals, which brings great harm to the aquaculture industry. The common mycotoxin adsorbents have low adsorption rates for DON, and the use of biological methods to remove DON in feeds has gradually become a research trend. One hundred and twenty crossbred barrows were randomly divided into four groups, which included the normal diet group (CON), normal diet + detoxifier group (Det), DON-polluted diet group (DON), and DON-polluted diet + DON detoxifier group (DON + Det); the experiment lasted for 28 d. The results showed that, compared with piglets fed a normal diet, those piglets fed DON-polluted diets significantly decreased their average daily gain (ADG) and average daily feed intake (ADFI) during the 1–14 d and 1–28 d periods; the content of immunoglobulin G (IgG), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), interleukin-4 (IL-4), and interleukin-10 (IL-10) in serum was decreased; and the content of aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA), diamine oxidase (DAO), and endotoxin (LPS) was increased in pigs fed DON-polluted diets; meanwhile, feeding piglets DON-polluted diets significantly reduced the levels of acetic acid, propionic acid, and total short-chain fatty acids (SCFAs) as well as gut microbiota health index (GMHI) in piglet faeces, but increased the relative abundance of Treponema, Prevotellaceae_UGG-001, Lachnospiraceae_XPB1014_group, Frisingicoccus and Sphaerochaeta. In contrast, the addition of a composite detoxifier effectively ameliorated the reduction in ADG and ADFI in piglets caused by DON-polluted diets. It suppressed the reduction in CAT, SOD, GSH-PX, IL-4, and IL-10 and the elevation of TNF-α, IL-2, IL-6, IL-12, MDA, LPS, and DAO in serum; the composite detoxifier also restrained the decrease in SCFA in piglet faeces and increased the relative abundance of Ruminococcus, Lachnospiraceae_NK4A136_group, Lachnospiraceae_AC2044_group, UCG-009, and Eubacterium_siraeum_group bacteria. The composite detoxifier effectively mitigated the adverse effects of a DON-polluted diet on piglet growth performance, blood biochemical indices, and gut microbiota composition. Full article
(This article belongs to the Section Molecular Microbiology)
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19 pages, 3855 KiB  
Article
Bone Morphogenetic Protein 7 Improves Wound Healing in Diabetes by Decreasing Inflammation and Promoting M2 Macrophage Polarization
by Jessica Da Silva, Ana Figueiredo, Yu-Hua Tseng, Eugenia Carvalho and Ermelindo C. Leal
Int. J. Mol. Sci. 2025, 26(5), 2036; https://doi.org/10.3390/ijms26052036 - 26 Feb 2025
Viewed by 64
Abstract
Diabetic foot ulcers (DFUs) are a devastating complication of diabetes, presenting limited treatment success rates due to their complex pathophysiology. Bone morphogenetic protein 7 (BMP7) confers tissue protective and regenerative functions, but its potential role in diabetic wound healing is unknown. The aim [...] Read more.
Diabetic foot ulcers (DFUs) are a devastating complication of diabetes, presenting limited treatment success rates due to their complex pathophysiology. Bone morphogenetic protein 7 (BMP7) confers tissue protective and regenerative functions, but its potential role in diabetic wound healing is unknown. The aim of this study was to investigate the effects of topical BMP7 treatment in wound healing using a streptozotocin-induced diabetic mouse model. The expression of markers of wound healing progression were detected using RT-PCR or immunohistochemistry. Overall, BMP7 improved wound closure, as well as maturation of granulation tissue and collagen deposition, as evidenced by hematoxylin and eosin and Masson’s trichrome histological analysis. The expression of inflammatory markers (IL-6, TNF-α) and matrix metalloproteinase-9 were decreased in BMP7-treated wounds, together with the number of pro-inflammatory M1 macrophages and T lymphocytes. The number of anti-inflammatory M2 macrophages was increased in BMP7-treated wounds. Moreover, BMP7 decreased oxidative stress and increased Ki67+ cells and CD31+ cells, indicating induced proliferation and angiogenesis in the wound bed compared to the control wounds. Finally, BMP7 activated the ERK pathway and suppressed the p38 pathway in diabetic wounds. Together, our data suggest that BMP7 enhanced skin wound healing in diabetes by decreasing local inflammation and oxidative stress, which promoted a regenerative environment for collagen deposition, wound maturation, cell proliferation, and angiogenesis. These findings underline BMP7 as a potential therapeutic agent for the treatment of skin wounds in diabetes. Full article
(This article belongs to the Special Issue Molecular and Cellular Perspectives on Wound Healing)
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14 pages, 694 KiB  
Article
Escala de Mitos do Amor Romântico (EMAR): Portuguese Version of the Scale of Myths of Romantic Love (SMRL)
by Jaume Grané-Morcillo, Susana Costa-Ramalho, Carles Pérez-Testor and Berta Vall
Soc. Sci. 2025, 14(3), 142; https://doi.org/10.3390/socsci14030142 - 26 Feb 2025
Viewed by 65
Abstract
Background: Violence legitimization has been associated with the myths of romantic love. In the Portuguese context, there are no available standardized tools to assess it. This study aimed to develop the adaptation and validation of the Portuguese version of the Scale of Myths [...] Read more.
Background: Violence legitimization has been associated with the myths of romantic love. In the Portuguese context, there are no available standardized tools to assess it. This study aimed to develop the adaptation and validation of the Portuguese version of the Scale of Myths of Romantic Love (SMRL) in youth and emerging adults. Also, the acceptance of myths of romantic love was described. Methods: The sample comprised 165 individuals (83% female) aged from 18 to 30 (M = 23.01; SD = 3.33). Results: Confirmatory factor analysis supported the two-factor correlated structure found in the original validation: Idealized Love (IL) and Distorted Love (DL). The selected nested model obtained acceptable good-of-fit indices. Reliability analysis indicated acceptable internal consistency (α = 0.728; ω = 0.711) and the item-factor correlations were strong. Gender differences in the endorsement of romantic myths were found. Males obtained significantly greater acceptance of need for a couple and ambivalence myths. Females significantly endorsed the half orange myth. Conclusions: Our findings supported the two-factor related structure of the SMRL Portuguese version. Gender-based differences in romantic beliefs and their potential link to violence legitimization, gender stereotypes, and love perception are highlighted as in need of further studies. This research provides evidence regarding the psychometric properties of the Escala de Mitos do Amor Romântico (EMAR). Full article
(This article belongs to the Section Gender Studies)
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22 pages, 6715 KiB  
Article
Anti-Inflammatory and Antioxidant Effects of White Grape Pomace Polyphenols on Isoproterenol-Induced Myocardial Infarction
by Raluca Maria Pop, Paul-Mihai Boarescu, Corina Ioana Bocsan, Mădălina Luciana Gherman, Veronica Sanda Chedea, Elena-Mihaela Jianu, Ștefan Horia Roșian, Ioana Boarescu, Floricuța Ranga, Liliana Lucia Tomoiagă, Alexandra Doina Sîrbu, Andrei Ungur, Marian Taulescu, Alina Elena Pârvu and Anca Dana Buzoianu
Int. J. Mol. Sci. 2025, 26(5), 2035; https://doi.org/10.3390/ijms26052035 - 26 Feb 2025
Viewed by 115
Abstract
Grape pomace (GP), the residue left after grape pressing in winemaking, is rich in polyphenols, including flavonoids, tannins, and phenolic acids, which have antioxidant and anti-inflammatory properties. The present study aimed to evaluate the cardioprotective effects of white grape pomace (WGP) extract in [...] Read more.
Grape pomace (GP), the residue left after grape pressing in winemaking, is rich in polyphenols, including flavonoids, tannins, and phenolic acids, which have antioxidant and anti-inflammatory properties. The present study aimed to evaluate the cardioprotective effects of white grape pomace (WGP) extract in two concentrations rich in polyphenols (795 mg polyphenols from WGP/kg body weight (bw) and 397.5 mg polyphenols from WGP/kg bw)), on isoproterenol (ISO)-induced myocardial infarction (MI), focusing on its anti-inflammatory and antioxidant effects. White grape pomace administration for 14 days offered a cardio-protective effect and prevented prolongation of the QT and QTc intervals on the electrocardiogram. Both concentrations of WGP prevented the elevation of nitric oxide (NO) and malondialdehyde (MDA) in the serum, with the best results being observed for the highest concentration (p < 0.05). White grape pomace administration offered a reduction in pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), and interleukin 1β (IL-1β) in both serum and tissue in a dose-dependent manner, with the highest WGP concentration having the best effect (p < 0.05). Moreover, WGP reduced histological changes associated with MI. The findings of the present study demonstrate that WGP extract exerts cardio protective effects by reducing MI-associated inflammation and oxidative stress. Full article
(This article belongs to the Section Bioactives and Nutraceuticals)
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15 pages, 3693 KiB  
Article
Two Complete Mitochondrial Genomes of Potamanthidae (Ephemeroptera): Genome Features and Phylogenetic Implications
by Ran Li, Chao Xue, Zhenxing Ma and Changfa Zhou
Diversity 2025, 17(3), 164; https://doi.org/10.3390/d17030164 - 26 Feb 2025
Viewed by 146
Abstract
The family Potamanthidae is widely distributed across the Holarctic and Oriental regions, with nymphs often utilized as bioindicators for water pollution and human-induced environmental disturbances. However, limited mitochondrial genomes (mitogenomes) have been reported for this family. This study presents the first complete mitochondrial [...] Read more.
The family Potamanthidae is widely distributed across the Holarctic and Oriental regions, with nymphs often utilized as bioindicators for water pollution and human-induced environmental disturbances. However, limited mitochondrial genomes (mitogenomes) have been reported for this family. This study presents the first complete mitochondrial genome sequences of two Potamanthidae species, Potamanthus longitibius and Rhoenanthus youi. The mitogenome sizes were 15,430 bp and 15,232 bp, with A + T contents of 68.79% and 66.75%, respectively. The most abundant amino acids were phenylalanine (Phe), isoleucine (Ile), glycine (Gly), and alanine (Ala), with leucine (Leu2) showing the highest relative synonymous codon usage (RSCU) value. The analysis of tRNA secondary structures revealed high conservation among the tRNAs encoded on the H-strand. Phylogenetic reconstruction, incorporating 40 species from 13 families of Ephemeroptera, confirmed the monophyly of all the families and supported a sister group relationship between Potamanthidae and Ephemeridae. Additionally, the sequence previously identified as Rhoenanthus sp. JZ-2021 was reassigned to the genus Potamanthus. This study provides valuable insights into the evolutionary relationships within Potamanthidae and lays a foundation for future phylogenetic and taxonomic research. Full article
(This article belongs to the Special Issue Freshwater Zoobenthos Biodiversity, Evolution and Ecology)
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23 pages, 769 KiB  
Review
Multifaceted Pathophysiology and Secondary Complications of Chronic Spinal Cord Injury: Focus on Pressure Injury
by Mario Martínez-Torija, Pedro F. Esteban, Angela Santos-de-la-Mata, Matilde Castillo-Hermoso, Eduardo Molina-Holgado and Rafael Moreno-Luna
J. Clin. Med. 2025, 14(5), 1556; https://doi.org/10.3390/jcm14051556 - 26 Feb 2025
Viewed by 144
Abstract
Background/Objectives: Spinal cord injury (SCI) is a complex medical condition with widespread effects that extend beyond motor and sensory impairments. In addition to nervous system damage, SCI patients experience various secondary complications, including vascular dysfunction, altered body composition, and metabolic disturbances. Among [...] Read more.
Background/Objectives: Spinal cord injury (SCI) is a complex medical condition with widespread effects that extend beyond motor and sensory impairments. In addition to nervous system damage, SCI patients experience various secondary complications, including vascular dysfunction, altered body composition, and metabolic disturbances. Among the most common secondary pathologies is the development of pressure injuries (PIs), chronic wounds that significantly affect quality of life and can be challenging to treat. Understanding the physiological and cellular mechanisms behind these complications is crucial for improving care and therapeutic outcomes. Methods: We conducted a comprehensive literature search in PubMed, Scopus, and Google Scholar using keywords related to spinal cord injury, pressure ulcer/pressure injuries, metabolic and vascular dysfunction, biomechanics, and regenerative therapies. Studies were selected based on their relevance to the pathophysiology, risk factors, and novel therapeutic approaches for PIs in SCI patients. Results: Vascular dysfunction, characterized by impaired blood flow and microcirculatory issues, predisposes SCI patients to ischemia and tissue necrosis, particularly in areas subjected to prolonged pressure. Additionally, changes in body composition, such as increased adiposity and muscle atrophy, further compromise tissue integrity and healing capacity. The inflammatory response, mediated by cytokines such as IL-1, IL-6, and TNF-α, exacerbates these effects by sustaining a pro-inflammatory environment that delays the transition of macrophages to the M2 phenotype, critical for wound healing. External factors, such as poor nutrition, infections, and immobility, also play a significant role in worsening the wound healing process. Conclusions: Chronic SCI induces a cascade of physiological changes that predispose patients to the development of PIs and complicate their recovery. The intricate interplay of vascular, metabolic, and inflammatory responses creates a hostile environment for wound healing. A deeper understanding of these systemic effects is essential not only for developing targeted therapeutic strategies to improve chronic wound healing but also for refining preventive approaches that minimize their occurrence. Advancing this knowledge will ultimately help enhance the quality of life for individuals with SCI. Full article
(This article belongs to the Special Issue Recent Advances in Therapy of Trauma and Surgical Critical Care)
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14 pages, 2903 KiB  
Article
Polysome Profiling Proves Impaired IL-10 and Caspase-8 Translation in PBMCs of Hemodialysis Patients
by Amanda Dawood, Roman Fiedler, Silke Markau, Matthias Girndt and Christof Ulrich
Biomolecules 2025, 15(3), 335; https://doi.org/10.3390/biom15030335 - 26 Feb 2025
Viewed by 79
Abstract
Triggered by uremic intoxication, a surplus of inflammatory mediators is present in the serum of hemodialysis (HD) patients. Anti-inflammatory counterbalancing mechanisms initiated by interleukin-10 (IL-10) and caspase-8 (Casp-8) appear to be disturbed. Earlier observations let us suppose that translational rather than transcriptional mechanisms [...] Read more.
Triggered by uremic intoxication, a surplus of inflammatory mediators is present in the serum of hemodialysis (HD) patients. Anti-inflammatory counterbalancing mechanisms initiated by interleukin-10 (IL-10) and caspase-8 (Casp-8) appear to be disturbed. Earlier observations let us suppose that translational rather than transcriptional mechanisms are responsible for this effect. Therefore, we investigated the polysome profiling of isolated PBMCs to study gene-specific mRNAs attached to monosomes and polysomes in HD patients (n = 42), patients with lipid disorder and normal renal function (LD, n = 10) and healthy control subjects (CO, n = 9). CRP (C-reactive protein) as a marker of inflammation was significantly elevated in HD and LD patients compared to CO subjects. NGAL (neutrophil-associated lipocalin), a potential marker of kidney disease and inflammation was increased in HD versus LD and CO. LD patients, however, had significantly higher proteosomal IL-10 and Casp-8 activities. LD and HD are two high cardiovascular risk groups with microinflammation. Lower translational activities of IL-10 and Casp-8 mRNAs in HD may be the result of a weak anti-inflammatory response potentially associated with the uremic immune defect. Full article
(This article belongs to the Special Issue Inflammation and Hemostasis: 2nd Edition)
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18 pages, 7868 KiB  
Article
A Combination of Anatolian Propolis and Curcumin Protects Fibroblasts Against Beclomethasone (Nazal Steroid)-Induced Oxidative Stress by Modulating IL-25, MMP-2, VEGF, and FGF-2 Expressions
by Tarik Yagci, Sidika Genc, Riza Dundar, Halil Ibrahim Altiner and Ali Taghizadehghalehjoughi
Pharmaceuticals 2025, 18(3), 326; https://doi.org/10.3390/ph18030326 - 26 Feb 2025
Viewed by 38
Abstract
Background: Nasal steroids are commonly prescribed in ear, nose, and throat clinics. It is observed that the use of nasal steroids is increasing due to the prevalence of allergic rhinitis. Because beclomethasone (BCM) toxicity is low, it is highly preferred in allergic rhinitis. [...] Read more.
Background: Nasal steroids are commonly prescribed in ear, nose, and throat clinics. It is observed that the use of nasal steroids is increasing due to the prevalence of allergic rhinitis. Because beclomethasone (BCM) toxicity is low, it is highly preferred in allergic rhinitis. The rate of toxicity increases with the increase in the duration and dose of BCM use. However, the protective mechanism of Anatolian propolis (AP) and curcumin (Cur) against BCM toxicity has not been fully explained. Aim: The study evaluates the potential BCM-induced toxicity effect on VEGF, MMP-2, IL-25, and IL-10 parameters after Cur and AP treatment. Materials and Methods: Cell viability, oxidative stress, and gene expression were used for toxicity evaluation. Results: AP 2.5 mg/mL and Cur 16 µg/mL show high viability and antioxidant capacity. BCM increased the levels of IL-25, IL-10, and MMP-2, and a decrease was detected in the expression levels of FGF-2 and VEGF. Conclusions: AP and Cur show effective healing, and AP has been shown to improve inflammation more effectively than Cur. However, the combination of AP and Cur significantly improved the induced toxicity effects. Full article
(This article belongs to the Section Natural Products)
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13 pages, 2029 KiB  
Article
Human Recombinant Interleukin-6 and Leukemia Inhibitory Factor Improve Inner Cell Mass Cell Number but Lack Cryoprotective Activities on In Vitro-Produced Bovine Blastocysts
by Mary A. Oliver, Kayla J. Alward, Michelle L. Rhoads and Alan D. Ealy
Animals 2025, 15(5), 668; https://doi.org/10.3390/ani15050668 - 25 Feb 2025
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Abstract
This work explored whether supplementing recombinant human interleukin-6 (IL6), interleukin-11 (IL11), or leukemia inhibitory factor (LIF) improves IVP bovine embryo development, morphology, and cryosurvivability. Embryos were treated from day 5 to 8 post-fertilization with either the carrier only (control) or 100 ng/mL of [...] Read more.
This work explored whether supplementing recombinant human interleukin-6 (IL6), interleukin-11 (IL11), or leukemia inhibitory factor (LIF) improves IVP bovine embryo development, morphology, and cryosurvivability. Embryos were treated from day 5 to 8 post-fertilization with either the carrier only (control) or 100 ng/mL of IL6, IL11, or LIF. Blastocyst formation and stage were determined on day 7 and 8. A subset of day 8 blastocysts was processed for immunofluorescence to count trophectoderm (TE) and inner cell mass (ICM) cell numbers and another subset was slow frozen and stored in liquid nitrogen until thawing. No differences in the blastocyst rate or blastocyst stage of development were detected. Increases in ICM cell numbers were observed for IL6 and LIF but not the IL11 treatment. None of the cytokine treatments applied before freezing affected post-thaw survival, TE or ICM cell number, or cell death 24 h after thawing. In conclusion, supplementing IL6 and LIF improves ICM cell numbers in non-frozen blastocysts, but there was no evidence that any of these cytokine treatments contain cryoprotective properties in bovine embryos. Full article
(This article belongs to the Special Issue Recent Advances in Reproductive Biotechnologies—Second Edition)
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