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28 pages, 4045 KiB  
Article
The Absence of a Very Long Chain Fatty Acid (VLCFA) in Lipid A Impairs Agrobacterium fabrum Plant Infection and Biofilm Formation and Increases Susceptibility to Environmental Stressors
by Iwona Komaniecka, Kamil Żebracki, Andrzej Mazur, Katarzyna Suśniak, Anna Sroka-Bartnicka, Anita Swatek and Adam Choma
Molecules 2025, 30(5), 1080; https://doi.org/10.3390/molecules30051080 (registering DOI) - 26 Feb 2025
Abstract
The Agrobacterium fabrum C58 is a phytopathogen able to infect numerous species of cultivated and ornamental plants. During infection, bacteria genetically transform plant cells and induce the formation of tumours at the site of invasion. Bacterial cell wall components play a crucial role [...] Read more.
The Agrobacterium fabrum C58 is a phytopathogen able to infect numerous species of cultivated and ornamental plants. During infection, bacteria genetically transform plant cells and induce the formation of tumours at the site of invasion. Bacterial cell wall components play a crucial role in the infection process. Lipopolysaccharide is the main component of Gram-negative bacteria's outer leaflet of outer membrane. Its lipophilic part, called lipid A, is built of di-glucosamine backbone substituted with a specific set of 3-hydroxyl fatty acids. A. fabrum incorporates a very long chain hydroxylated fatty acid (VLCFA), namely 27-hydroxyoctacosanoic acid (28:0-(27OH)), into its lipid A. A. fabrum C58 mutants deprived of this component due to mutation in the VLCFA’s genomic region, have been characterised. High-resolution mass spectrometry was used to establish acylation patterns in the mutant’s lipid A preparations. The physiological properties of mutants, as well as their motility, ability to biofilm formation and plant infectivity, were tested. The results obtained showed that the investigated mutants were more sensitive to environmental stress conditions, formed a weakened biofilm, exhibited impaired swimming motility and were less effective in infecting tomato seedlings compared to the wild strain. Full article
14 pages, 527 KiB  
Review
Effect of Omega-3 in Patients Undergoing Bone Marrow Transplantation: A Narrative Review
by Stefano Mancin, Marco Sguanci, Gaetano Ferrara, Riccardo Caccialanza, Emanuele Cereda, Alessio Lo Cascio, Mauro Parozzi, Fabio Petrelli, Giovanni Cangelosi and Sara Morales Palomares
Hemato 2025, 6(1), 5; https://doi.org/10.3390/hemato6010005 - 26 Feb 2025
Abstract
Background/Aim: Omega-3 fatty acids, a key component of immunonutrition, have been used to modulate immune responses and improve clinical outcomes in various settings, including hematological patients undergoing hematopoietic stem cell transplantation (HSCT). This study aimed to summarize the effects of omega-3 supplementation on [...] Read more.
Background/Aim: Omega-3 fatty acids, a key component of immunonutrition, have been used to modulate immune responses and improve clinical outcomes in various settings, including hematological patients undergoing hematopoietic stem cell transplantation (HSCT). This study aimed to summarize the effects of omega-3 supplementation on inflammation, long-term survival, and post-transplant complications, such as graft-versus-host disease (GVHD) and mucositis. Additionally, its impact on nutritional status and immune function was considered. Methods: A narrative review was conducted. The PubMed, Embase, and CINAHL databases were searched, along with sources of gray literature. From 2607 records, five studies met the inclusion criteria and were analyzed Results: The available literature suggests that omega-3 supplementation provides significant benefits, including reduced inflammation, lower C-reactive protein levels, and improved long-term survival. Furthermore, a reduction in GVHD and mucositis incidence was observed. The safety profile of omega-3 was favorable, with no serious adverse events reported. However, the evidence remains limited and heterogeneous, emphasizing the need for further well-designed trials to validate and expand upon these findings. Conclusions: Omega-3 fatty acids appear to be a promising intervention for improving clinical outcomes in HSCT patients. Additional research is essential to optimize treatment protocols and confirm its immunomodulatory role. Full article
10 pages, 385 KiB  
Article
Effect of Coffee Silverskin on Meat Quality of Growing Rabbits
by Francesco Foti, Manuel Scerra, Pasquale Caparra, Matteo Bognanno, Caterina Cilione, Paolo Fortugno, Paolo De Caria, Valerio Chinè, Guido Mangione, Salvatore Gagliano and Luigi Chies
Foods 2025, 14(5), 812; https://doi.org/10.3390/foods14050812 (registering DOI) - 26 Feb 2025
Viewed by 11
Abstract
The aim of the present study was to assess the impacts of coffee silverskin (CSS) inclusion in rabbit diets in regard to meat quality. A total of 30 Hycole rabbits were divided into two groups of 15 animals per group and fed with [...] Read more.
The aim of the present study was to assess the impacts of coffee silverskin (CSS) inclusion in rabbit diets in regard to meat quality. A total of 30 Hycole rabbits were divided into two groups of 15 animals per group and fed with a basal diet (C group) or with the same basal diet but with 10% of CSS (CSS10 group) as a partial cereal replacement. Integration of 10% CSS in the rabbit diet increased dry matter intake (DMI, p < 0.05) and tended to increase (p = 0.096) the final body weight. The CSS diet tended to reduce the concentration of C18:3 ω-3 (p = 0.089), C20:5 ω-3 (p = 0.064) and C22:5 ω-3 (p = 0.069) in muscle compared to the control diet, negatively affecting the ω-6/ω-3 ratio (p < 0.05), which was higher in the CSS10 group compared to the control group. Finally, the addition of CSS to the rabbit diet made the meat more resistant (p < 0.01) to lipid oxidation. Further research is needed to better understand the reasons for improved oxidative stability in meat following dietary CSS supplementation. Full article
(This article belongs to the Section Meat)
11 pages, 679 KiB  
Review
How Close Are We to the Production of Milk in Alternative Systems? The Fat Perspective
by Roni Tadmor-Levi and Nurit Argov-Argaman
Foods 2025, 14(5), 809; https://doi.org/10.3390/foods14050809 (registering DOI) - 26 Feb 2025
Abstract
The growing demand for sustainable food systems has led to significant advancements in developing alternatives to animal-derived products. Dairy products are an important dietary source of proteins and fats; however, their production raises environmental concerns, including greenhouse gas emissions, extensive land and water [...] Read more.
The growing demand for sustainable food systems has led to significant advancements in developing alternatives to animal-derived products. Dairy products are an important dietary source of proteins and fats; however, their production raises environmental concerns, including greenhouse gas emissions, extensive land and water usage, and biodiversity loss. Therefore, there is a need to develop sustainable, scalable solutions that will enable the production of quality replacements for animal-based foods with reduced environmental impacts. Recognizing that replacing animal-based products from a single source is currently not feasible; there is a need for high-quality sources of ingredients that can be combined to mimic the holistic product. In recent years, plant-based dairy alternatives have gained traction; however, their inability to replicate the sensorial experience of real milk—attributed largely to the unique composition and structure of milk fat—remains a key limitation. Cow’s milk fat has distinctive characteristics, including a complex fatty acid profile, which is rich in short- and medium-chain saturated fatty acids with specific positional distribution. These characteristics of cow’s milk play a role in delivering the aroma, texture, and mouthfeel of dairy products. Recent efforts have focused on leveraging precision fermentation and cellular agriculture to mimic these properties. This review explores the unique lipid composition of ruminant milk, the biosynthesis of milk fats, and the challenges of replicating these features in non-mammalian systems. Emphasis is placed on short-chain fatty acids and chain-termination mechanisms in fatty acid synthesis. By integrating insights from diverse biological systems, we aim to contribute to a deeper understanding of the complex processes related to milk fat synthesis. Full article
(This article belongs to the Special Issue Quality Ingredients for the Animal Products Alternatives Industry)
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15 pages, 4072 KiB  
Article
Postbiotic Sodium Butyrate Mitigates Hypertension and Kidney Dysfunction in Juvenile Rats Exposed to Microplastics
by You-Lin Tain, Ying-Jui Lin, Chih-Yao Hou, Guo-Ping Chang-Chien, Shu-Fen Lin and Chien-Ning Hsu
Antioxidants 2025, 14(3), 276; https://doi.org/10.3390/antiox14030276 - 26 Feb 2025
Abstract
Background: Plastic production has led to widespread microplastic (MP) pollution, with children more vulnerable to MPs than adults. However, the mechanisms linking MP exposure to hypertension and kidney disease in children remain unclear. This study explored whether sodium butyrate, a short-chain fatty acid [...] Read more.
Background: Plastic production has led to widespread microplastic (MP) pollution, with children more vulnerable to MPs than adults. However, the mechanisms linking MP exposure to hypertension and kidney disease in children remain unclear. This study explored whether sodium butyrate, a short-chain fatty acid (SCFA) with antioxidant and anti-inflammatory properties, could mitigate MP-induced hypertension and kidney damage in juvenile rats. Methods: Male Sprague-Dawley rats (3 weeks old) were randomly assigned to four groups (n = 8/group): control, low-dose MP (1 mg/L), high-dose MP (10 mg/L), and high-dose MP with sodium butyrate (400 mg/kg/day). Rats were euthanized at 12 weeks. Results: High-dose MP exposure impaired kidney function and increased blood pressure, which were alleviated by sodium butyrate through reduced oxidative stress, modulation of gut microbiota, increased plasma butyric acid levels, and enhanced renal SCFA-sensing G protein-coupled receptor 43 expression. Conclusions: Sodium butyrate holds potential for mitigating MP-induced hypertension by reducing oxidative stress, modulating the gut microbiota, and elevating butyric acid levels. Full article
(This article belongs to the Special Issue Environmental Pollution and Oxidative Stress)
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19 pages, 5768 KiB  
Article
Weizmannia coagulans BC99: A Novel Adjunct to Protein Supplementation for Enhancing Exercise Endurance and Reducing Fatigue
by Minghan Guo, Lina Zhao, Li Cao, Xuan Li, Jie Zhang, Yao Dong, Ying Wu and Shaobin Gu
Foods 2025, 14(5), 801; https://doi.org/10.3390/foods14050801 (registering DOI) - 26 Feb 2025
Viewed by 82
Abstract
Adequate protein consumption is essential for optimal physical fitness and enhancing athletic performance. This study explored the impact of Weizmannia coagulans BC99 on protein-supplemented male fatigued mice, examining aspects such as protein digestion, exercise endurance, fatigue-related biochemistry, oxidative stress, and gut microbiota alterations. [...] Read more.
Adequate protein consumption is essential for optimal physical fitness and enhancing athletic performance. This study explored the impact of Weizmannia coagulans BC99 on protein-supplemented male fatigued mice, examining aspects such as protein digestion, exercise endurance, fatigue-related biochemistry, oxidative stress, and gut microbiota alterations. Results indicate that the synergistic effect of probiotics and protein significantly boosts the activity of protein-digesting enzymes, enhances protein absorption, and reduces serum levels of urea nitrogen, lactate, lactate dehydrogenase, creatine kinase, malondialdehyde, and the inflammatory cytokines interleukin-1β and interleukin-6 in skeletal muscle. Additionally, serum catalase, glutathione, superoxide dismutase levels, interleukin-4 in skeletal muscle, and glycogen stores in muscle and liver were notably increased. The study also found elevated mRNA expression levels of Nrf2 and HO-1 in skeletal muscle. Furthermore, an increase in short-chain fatty acids was observed in the probiotic treatment group, and 16S rDNA sequencing revealed that Weizmannia coagulans BC99 enhanced gut microbiota diversity and augmented beneficial bacterial populations including Roseburia, Mucispirillum, Rikenella, and Kineothrix. Collectively, these findings suggest that combining BC99 with protein supplementation can effectively improve gut flora, thereby enhancing exercise capacity and exerting potent anti-fatigue effects. Our research provides a new possibility for alleviating exercise-induced fatigue. Full article
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19 pages, 2329 KiB  
Article
Effects of Deoxynivalenol Detoxifier on Growth Performance, Blood Biochemical Indices, and Microbiota Composition of Piglets
by Luyao Zhang, Yongwei Wang, Weiwei Wang, Li Wang, Jingjing Shi, Junlin Cheng, Jing Zhang, Aike Li, Beibei He and Zhiyong Fan
Int. J. Mol. Sci. 2025, 26(5), 2045; https://doi.org/10.3390/ijms26052045 - 26 Feb 2025
Viewed by 64
Abstract
Deoxynivalenol (DON), also known as vomitoxin, has a high detection and exceeding rate in feed and is prone to causing symptoms such as loss of appetite, weight loss, vomiting, and diarrhoea in animals, which brings great harm to the aquaculture industry. The common [...] Read more.
Deoxynivalenol (DON), also known as vomitoxin, has a high detection and exceeding rate in feed and is prone to causing symptoms such as loss of appetite, weight loss, vomiting, and diarrhoea in animals, which brings great harm to the aquaculture industry. The common mycotoxin adsorbents have low adsorption rates for DON, and the use of biological methods to remove DON in feeds has gradually become a research trend. One hundred and twenty crossbred barrows were randomly divided into four groups, which included the normal diet group (CON), normal diet + detoxifier group (Det), DON-polluted diet group (DON), and DON-polluted diet + DON detoxifier group (DON + Det); the experiment lasted for 28 d. The results showed that, compared with piglets fed a normal diet, those piglets fed DON-polluted diets significantly decreased their average daily gain (ADG) and average daily feed intake (ADFI) during the 1–14 d and 1–28 d periods; the content of immunoglobulin G (IgG), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), interleukin-4 (IL-4), and interleukin-10 (IL-10) in serum was decreased; and the content of aminotransferase (AST), alanine aminotransferase (ALT), malondialdehyde (MDA), diamine oxidase (DAO), and endotoxin (LPS) was increased in pigs fed DON-polluted diets; meanwhile, feeding piglets DON-polluted diets significantly reduced the levels of acetic acid, propionic acid, and total short-chain fatty acids (SCFAs) as well as gut microbiota health index (GMHI) in piglet faeces, but increased the relative abundance of Treponema, Prevotellaceae_UGG-001, Lachnospiraceae_XPB1014_group, Frisingicoccus and Sphaerochaeta. In contrast, the addition of a composite detoxifier effectively ameliorated the reduction in ADG and ADFI in piglets caused by DON-polluted diets. It suppressed the reduction in CAT, SOD, GSH-PX, IL-4, and IL-10 and the elevation of TNF-α, IL-2, IL-6, IL-12, MDA, LPS, and DAO in serum; the composite detoxifier also restrained the decrease in SCFA in piglet faeces and increased the relative abundance of Ruminococcus, Lachnospiraceae_NK4A136_group, Lachnospiraceae_AC2044_group, UCG-009, and Eubacterium_siraeum_group bacteria. The composite detoxifier effectively mitigated the adverse effects of a DON-polluted diet on piglet growth performance, blood biochemical indices, and gut microbiota composition. Full article
(This article belongs to the Section Molecular Microbiology)
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17 pages, 3584 KiB  
Article
Comparative Analysis of Egg Yolk Phospholipid Unsaturation and Its Impact on Neural Health in Alzheimer Disease Mice
by Yuhang Sun, Yao Wu, Bing Fang, Jingyu Li, Yue Liu, Haina Gao and Ming Zhang
Foods 2025, 14(5), 792; https://doi.org/10.3390/foods14050792 - 26 Feb 2025
Viewed by 147
Abstract
The mechanism of egg yolk phosphatidylcholine (PC) in alleviating Alzheimer’s disease (AD) has not yet been clear. The fatty acid composition of PC, especially the ratio of polyunsaturated fatty acids (PUFA), may be a critical determinant of their structural and functional roles. This [...] Read more.
The mechanism of egg yolk phosphatidylcholine (PC) in alleviating Alzheimer’s disease (AD) has not yet been clear. The fatty acid composition of PC, especially the ratio of polyunsaturated fatty acids (PUFA), may be a critical determinant of their structural and functional roles. This study aimed to conduct a comparative analysis of the unsaturation levels of egg yolk PC and their impact on neurological health in a murine model of AD. The results showed that oral administration of high and low unsaturation PC (HUP, LUP) enhanced learning and memory abilities in AD mice, with the HUP intervention demonstrating superior efficacy compared to the LUP. Follow-up biochemical analysis of the brain tissue also suggested that HUP intervention effectively mitigated oxidative-stress damage and inhibited tau hyperphosphorylation in AD mice. Meanwhile, lipidomic analyses of the mouse hippocampus revealed that HUP intervention substantially increased the levels of phospholipids, such as PEt (phosphatidylethanol) and BisMePA (bis(methylthio)phenylacetic acid), which are recognized as vital components of neuronal cell membranes. Furthermore, HUP intervention markedly elevated the levels of phospholipids incorporating PUFAs in the hippocampus. These results revealed a mitigating role for unsaturated egg yolk PC in AD prevention and offer new insights into AD prevention from a lipidomic perspective. Full article
(This article belongs to the Special Issue Eggs and Egg Products: Production, Processing, and Safety)
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15 pages, 1180 KiB  
Article
Untargeted Lipidomic Biomarkers for Liver Cancer Diagnosis: A Tree-Based Machine Learning Model Enhanced by Explainable Artificial Intelligence
by Cemil Colak, Fatma Hilal Yagin, Abdulmohsen Algarni, Ali Algarni, Fahaid Al-Hashem and Luca Paolo Ardigò
Medicina 2025, 61(3), 405; https://doi.org/10.3390/medicina61030405 - 26 Feb 2025
Viewed by 201
Abstract
Background and Objectives: Liver cancer ranks among the leading causes of cancer-related mortality, necessitating the development of novel diagnostic methods. Deregulated lipid metabolism, a hallmark of hepatocarcinogenesis, offers compelling prospects for biomarker identification. This study aims to employ explainable artificial intelligence (XAI) [...] Read more.
Background and Objectives: Liver cancer ranks among the leading causes of cancer-related mortality, necessitating the development of novel diagnostic methods. Deregulated lipid metabolism, a hallmark of hepatocarcinogenesis, offers compelling prospects for biomarker identification. This study aims to employ explainable artificial intelligence (XAI) to identify lipidomic biomarkers for liver cancer and to develop a robust predictive model for early diagnosis. Materials and Methods: This study included 219 patients diagnosed with liver cancer and 219 healthy controls. Serum samples underwent untargeted lipidomic analysis with LC-QTOF-MS. Lipidomic data underwent univariate and multivariate analyses, including fold change (FC), t-tests, PLS-DA, and Elastic Network feature selection, to identify significant biomarker candidate lipids. Machine learning models (AdaBoost, Random Forest, Gradient Boosting) were developed and evaluated utilizing these biomarkers to differentiate liver cancer. The AUC metric was employed to identify the optimal predictive model, whereas SHAP was utilized to achieve interpretability of the model’s predictive decisions. Results: Notable alterations in lipid profiles were observed: decreased sphingomyelins (SM d39:2, SM d41:2) and increased fatty acids (FA 14:1, FA 22:2) and phosphatidylcholines (PC 34:1, PC 32:1). AdaBoost exhibited a superior classification performance, achieving an AUC of 0.875. SHAP identified PC 40:4 as the most efficacious lipid for model predictions. The SM d41:2 and SM d36:3 lipids were specifically associated with an increased risk of low-onset cancer and elevated levels of the PC 40:4 lipid. Conclusions: This study demonstrates that untargeted lipidomics, in conjunction with explainable artificial intelligence (XAI) and machine learning, may effectively identify biomarkers for the early detection of liver cancer. The results suggest that alterations in lipid metabolism are crucial to the progression of liver cancer and provide valuable insights for incorporating lipidomics into precision oncology. Full article
(This article belongs to the Special Issue Insights and Advances in Cancer Biomarkers)
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26 pages, 9912 KiB  
Article
Therapeutic Potential of Cranberry Proanthocyanidins in Addressing the Pathophysiology of Metabolic Syndrome: A Scrutiny of Select Mechanisms of Action
by Francis Feldman, Mireille Koudoufio, Alain Théophile Sané, Valérie Marcil, Mathilde Foisy Sauvé, James Butcher, Natalie Patey, Catherine Martel, Schohraya Spahis, Haonan Duan, Daniel Figeys, Yves Desjardins, Alain Stintzi and Emile Levy
Antioxidants 2025, 14(3), 268; https://doi.org/10.3390/antiox14030268 - 26 Feb 2025
Viewed by 79
Abstract
Metabolic syndrome (MetS) constitutes a spectrum of interconnected conditions comprising obesity, dyslipidemia, hypertension, and insulin resistance (IR). While a singular, all-encompassing treatment for MetS remains elusive, an integrative approach involving tailored lifestyle modifications and emerging functional food therapies holds promise in preventing its [...] Read more.
Metabolic syndrome (MetS) constitutes a spectrum of interconnected conditions comprising obesity, dyslipidemia, hypertension, and insulin resistance (IR). While a singular, all-encompassing treatment for MetS remains elusive, an integrative approach involving tailored lifestyle modifications and emerging functional food therapies holds promise in preventing its multifaceted manifestations. Our main objective was to scrutinize the efficacy of cranberry proanthocyanidins (PAC, 200 mg/kg/day for 12 weeks) in mitigating MetS pathophysiology in male mice subjected to standard Chow or high-fat/high-fructose (HFHF) diets while unravelling intricate mechanisms. The administration of PAC, in conjunction with an HFHF diet, significantly averted obesity, evidenced by reductions in body weight, adiposity across various fat depots, and adipocyte hypertrophy. Similarly, PAC prevented HFHF-induced hyperglycemia and hyperinsulinemia while also lessening IR. Furthermore, PAC proved effective in alleviating key risk factors associated with cardiovascular diseases by diminishing plasma saturated fatty acids, as well as levels of triglycerides, cholesterol, and non-HDL-C levels. The rise in adiponectin and drop in circulating levels of inflammatory markers showcased PAC’s protective role against inflammation. To better clarify the mechanisms behind PAC actions, gut–liver axis parameters were examined, showing significant enhancements in gut microbiota composition, microbiota-derived metabolites, and marked reductions in intestinal and hepatic inflammation, liver steatosis, and key biomarkers associated with endoplasmic reticulum (ER) stress and lipid metabolism. This study enhances our understanding of the complex mechanisms underlying the development of MetS and provides valuable insights into how PAC may alleviate cardiometabolic dysfunction in HFHF mice. Full article
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18 pages, 2871 KiB  
Article
Unveiling the Mechanism of Action of Palmitic Acid, a Human Topoisomerase 1B Inhibitor from the Antarctic Sponge Artemisina plumosa
by Alessio Ottaviani, Davide Pietrafesa, Bini Chhetri Soren, Jagadish Babu Dasari, Stine S. H. Olsen, Beatrice Messina, Francesco Demofonti, Giulia Chicarella, Keli Agama, Yves Pommier, Blasco Morozzo della Rocca, Federico Iacovelli, Alice Romeo, Mattia Falconi, Bill J. Baker and Paola Fiorani
Int. J. Mol. Sci. 2025, 26(5), 2018; https://doi.org/10.3390/ijms26052018 - 26 Feb 2025
Viewed by 41
Abstract
Cancer remains a leading cause of death worldwide, highlighting the urgent need for novel and more effective treatments. Natural products, with their structural diversity, represent a valuable source for the discovery of anticancer compounds. In this study, we screened 750 Antarctic extracts to [...] Read more.
Cancer remains a leading cause of death worldwide, highlighting the urgent need for novel and more effective treatments. Natural products, with their structural diversity, represent a valuable source for the discovery of anticancer compounds. In this study, we screened 750 Antarctic extracts to identify potential inhibitors of human topoisomerase 1 (hTOP1), a key enzyme in DNA replication and repair, and a target of cancer therapies. Bioassay-guided fractionation led to the identification of palmitic acid (PA) as the active compound from the Antarctic sponge Artemisina plumosa, selectively inhibiting hTOP1. Our results demonstrate that PA irreversibly blocks hTOP1-mediated DNA relaxation and specifically inhibits the DNA religation step of the enzyme’s catalytic cycle. Unlike other fatty acids, PA exhibited unique specificity, which we confirmed through comparisons with linoleic acid. Molecular dynamics simulations and binding assays further suggest that PA interacts with hTOP1-DNA complexes, enhancing the inhibitory effect in the presence of camptothecin (CPT). These findings identify PA as a hTOP1 inhibitor with potential therapeutic implications, offering a distinct mechanism of action that could complement existing cancer therapies. Full article
(This article belongs to the Special Issue Discovering Novel Bioactive Compounds Against Cancers)
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12 pages, 4034 KiB  
Article
Study of Intracellular Peptides of the Central Nervous System of Zebrafish (Danio rerio) in a Parkinson’s Disease Model
by Louise O. Fiametti, Camilla A. Franco, Leticia O. C. Nunes, Leandro M. de Castro and Norival A. Santos-Filho
Int. J. Mol. Sci. 2025, 26(5), 2017; https://doi.org/10.3390/ijms26052017 - 26 Feb 2025
Viewed by 26
Abstract
Although peptides have been shown to have biological functions in neurodegenerative diseases, their role in Parkinson’s disease has been understudied. A previous study by our group, which used a 6-hydroxydopamine zebrafish model, suggested that nine intracellular peptides may play a part in this [...] Read more.
Although peptides have been shown to have biological functions in neurodegenerative diseases, their role in Parkinson’s disease has been understudied. A previous study by our group, which used a 6-hydroxydopamine zebrafish model, suggested that nine intracellular peptides may play a part in this condition. In this context, our aim is to better understand the role of five of these nine peptides. The selection of peptides was made based on their precursor proteins, which are fatty acid binding protein 7, mitochondrial ribosomal protein S36, MARCKS-related protein 1-B, excitatory amino acid transporter 2 and thymosin beta-4. The peptides were chemically synthesized in solid phase and characterized by high-performance liquid chromatography and mass spectrometry. Circular dichroism was performed to determine the secondary structure of each peptide, which showed that all five peptides maintain a random structure in the aqueous solutions that were studied. Two molecules show a helical profile in trifluoroethanol, a known structuring agent. Cell viability by the MTT assay indicates that all five peptides are not cytotoxic in all concentrations tested in both mouse and human cell lines. Behavioral assay using a 6-OHDA zebrafish larvae model suggest that all peptides help in the recovery of motor function with 24 h treatment at two concentrations. Three peptides showed a complete recovery from the 6-OHDA-induced motor impairment. Further studies are needed to better understand the mechanism of action of these peptides and whether they are truly a potential ally against Parkinson’s disease. Full article
(This article belongs to the Special Issue New Challenges of Parkinson’s Disease)
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41 pages, 4070 KiB  
Article
Defined Diets Link Iron and α-Linolenic Acid to Cyp1b1 Regulation of Neonatal Liver Development Through Srebp Forms and LncRNA H19
by Colin R. Jefcoate, Michele C. Larsen, Yong-Seok Song, Meghan Maguire and Nader Sheibani
Int. J. Mol. Sci. 2025, 26(5), 2011; https://doi.org/10.3390/ijms26052011 - 25 Feb 2025
Viewed by 166
Abstract
Cyp1b1 substantially affects hepatic vascular and stellate cells (HSC) with linkage to liver fibrosis. Despite minimal hepatocyte expression, Cyp1b1 deletion substantially impacts liver gene expression at birth and weaning. The appreciable Cyp1b1 expression in surrounding embryo mesenchyme, during early organogenesis, provides a likely [...] Read more.
Cyp1b1 substantially affects hepatic vascular and stellate cells (HSC) with linkage to liver fibrosis. Despite minimal hepatocyte expression, Cyp1b1 deletion substantially impacts liver gene expression at birth and weaning. The appreciable Cyp1b1 expression in surrounding embryo mesenchyme, during early organogenesis, provides a likely source for Cyp1b1. Here defined breeder diets established major interconnected effects on neonatal liver of α-linolenic acid (ALA), vitamin A deficiency (VAD) and suboptimal iron fed mice. At birth Cyp1b1 deletion and VAD each activated perinatal HSC, while suppressing iron control by hepcidin. Cyp1b1 deletion also advanced the expression of diverse genes linked to iron regulation. Postnatal stimulations of Srebp-regulated genes in the fatty acid and cholesterol biosynthesis pathways were suppressed by Cyp1b1-deficiency. LncRNA H19 and the neutrophil alarmin S100a9 expression increased due to slower postnatal decline with Cyp1b1 deficiency. VAD reversed each of Cyp1b1 effect, probably due to enhanced HSC release of Apo-Rbp4. At birth, Cyp1b1 deletion enhanced H19 participation. Notably, a suppressor (Cnot3) decreased while an activity partner (Ezh2/H3K methylation) increased H19 expression. ALA elevated hepcidin mRNA and countered the inhibitory effects of Cyp1b1 deletion on hepcidin expression. Oxylipin metabolites of ALA from highly expressed hepatic Cyps are potential mediators. Cyp expression patterns demonstrated female dimorphism for neonatal liver. Mothers followed one of three fetal growth support programs probably linked to maturity at conception. Full article
(This article belongs to the Special Issue Cytochrome P450 Mechanism and Reactivity)
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12 pages, 2018 KiB  
Article
Evaluation of Red Yeast Rice Residue as an Alternative Feed Ingredient in Growing-Finishing Pig Diets
by Zhengqun Liu, Longbo Zhu, Ning Li, Zi Zheng, Chunyan Xie, Shiyue Liang, Jun Yan and Shuqin Mu
Agriculture 2025, 15(5), 495; https://doi.org/10.3390/agriculture15050495 - 25 Feb 2025
Viewed by 217
Abstract
This study aimed to evaluate the effects of graded levels of red yeast rice residue (RYRR) on the growth performance, nutrient digestibility, and fecal microbiota of growing-finishing pigs. A total of 144 pigs were randomly allocated into four dietary treatment groups, over a [...] Read more.
This study aimed to evaluate the effects of graded levels of red yeast rice residue (RYRR) on the growth performance, nutrient digestibility, and fecal microbiota of growing-finishing pigs. A total of 144 pigs were randomly allocated into four dietary treatment groups, over a 75-day experimental period. The study comprised a control group and three dietary supplementation groups receiving RYRR. The control (CON) group was fed a two-phase diet tailored to the pigs’ body weight, while the RYRR groups were provided with the CON diet, where corn, soybean meal, puffed soybeans, and wheat shorts were substituted with 5%, 10%, and 20% of RYRR. Supplementation with 10% RYRR enhanced the apparent digestibility of gross energy, dry matter, and crude fiber, while reducing the feed-to-gain ratio and serum triglyceride levels (p < 0.05). Microbiological analyses revealed that short-chain fatty acid-producing bacteria (Anaerotignum and Lachnospiraceae_UCG-009) were biomarkers in pigs fed the RYRR supplementation diets (p < 0.05). These results demonstrated that RYRR supplementation of the diet exerted beneficial effects on promoting nutrient digestibility as well as modulating the fecal microbiota of pigs, and the recommended proportion of RYRR added to the growing-finishing pigs’ diet is 10%. Full article
(This article belongs to the Special Issue Assessment of Nutritional Value of Animal Feed Resources)
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16 pages, 3598 KiB  
Article
Rapeseed and Palm Oils Can Improve the Growth, Muscle Texture, Fatty Acids and Volatiles of Marine Teleost Golden Pompano Fed Low Fish Oil Diets
by Fang Chen, Yunkun Lou, Junfeng Guan, Xue Lan, Zeliang Su, Chao Xu, Yuanyou Li and Dizhi Xie
Foods 2025, 14(5), 788; https://doi.org/10.3390/foods14050788 - 25 Feb 2025
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Abstract
This study evaluated the effects of different lipid sources—fish oil (FO), soybean oil, rapeseed oil, and palm oil—on the growth and muscle quality of golden pompano (Trachinotus ovatus) cultured in offshore cages for 10 weeks. Three diets (D1–D3) were formulated: D1 [...] Read more.
This study evaluated the effects of different lipid sources—fish oil (FO), soybean oil, rapeseed oil, and palm oil—on the growth and muscle quality of golden pompano (Trachinotus ovatus) cultured in offshore cages for 10 weeks. Three diets (D1–D3) were formulated: D1 used only fish oil, D2 blended fish, rapeseed oil, and palm oil, and D3 combined fish and soybean oils. Fish in the D1 group showed the highest weight gain, specific growth rate, and muscle protein content, significantly outperforming D3. No significant differences in muscle lipid content or edible quality were found between groups. D1 had the highest levels of long-chain and n-3 polyunsaturated fatty acids (PUFA), while D3 had higher n-6 PUFA. Saturated and monounsaturated fatty acids were higher in D1 and D2 than in D3. Muscle volatiles like aldehydes and amines were elevated in D1, with more pleasant flavors compared to D2 and D3. Muscle texture was superior in D2. These results suggest that rapeseed and palm oils can enhance growth, flavor, and texture in fish on low FO diets, offering a sustainable alternative to reduce reliance on marine-based feed in aquaculture. Full article
(This article belongs to the Special Issue Latest Research on Flavor Components and Sensory Properties of Food)
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