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18 pages, 3674 KiB  
Article
Detection and Quantification of Vegetation Losses with Sentinel-2 Images Using Bi-Temporal Analysis of Spectral Indices and Transferable Random Forest Model
by Alicja Rynkiewicz, Agata Hościło, Linda Aune-Lundberg, Anne B. Nilsen and Aneta Lewandowska
Remote Sens. 2025, 17(6), 979; https://doi.org/10.3390/rs17060979 (registering DOI) - 11 Mar 2025
Abstract
The precise spatially explicit data on land cover and land use changes is one of the essential variables for enhancing the quantification of greenhouse gas emissions and removals, which is relevant for meeting the goal of the European economy and society to become [...] Read more.
The precise spatially explicit data on land cover and land use changes is one of the essential variables for enhancing the quantification of greenhouse gas emissions and removals, which is relevant for meeting the goal of the European economy and society to become climate-neutral by 2050. The accuracy of the machine learning models trained on remote-sensed data suffers from a lack of reliable training datasets and they are often site-specific. Therefore, in this study, we proposed a method that integrates the bi-temporal analysis of the combination of spectral indices that detects the potential changes, which then serve as reference data for the Random Forest classifier. In addition, we examined the transferability of the pre-trained model over time, which is an important aspect from the operational point of view and may significantly reduce the time required for the preparation of reliable and accurate training data. Two types of vegetation losses were identified: woody coverage converted to non-woody vegetation, and vegetated areas converted to sealed surfaces or bare soil. The vegetation losses were detected annually over the period 2018–2021 with an overall accuracy (OA) above 0.97 and a Kappa coefficient of 0.95 for all time intervals in the study regions in Poland and Norway. Additionally, the pre-trained model’s temporal transferability revealed an improvement of the OA by 5 percentage points and the macroF1-Score value by 12 percentage points compared to the original model. Full article
(This article belongs to the Special Issue Women’s Special Issue Series: Remote Sensing 2023-2025)
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21 pages, 8372 KiB  
Article
Audio-Visual Learning for Multimodal Emotion Recognition
by Siyu Fan, Jianan Jing and Chongwen Wang
Symmetry 2025, 17(3), 418; https://doi.org/10.3390/sym17030418 (registering DOI) - 11 Mar 2025
Abstract
Most current emotion recognition methods are often limited to a single- or dual-modality approach, neglecting the rich information embedded in other modalities. This limitation hampers the accurate identification of complex or subtle emotional expressions. Additionally, to reduce the computational cost during inference, minimizing [...] Read more.
Most current emotion recognition methods are often limited to a single- or dual-modality approach, neglecting the rich information embedded in other modalities. This limitation hampers the accurate identification of complex or subtle emotional expressions. Additionally, to reduce the computational cost during inference, minimizing the model’s parameter size is essential. To address these challenges, we utilize the concept of symmetry to design a balanced multimodal architecture that integrates facial expressions, speech, and body posture information, aiming to enhance both recognition performance and computational efficiency. By leveraging the E-Branchformer network and using the F1- score as the primary performance evaluation metric, the experiments are mainly conducted on the CREMA-D corpora. The experimental results demonstrate that the proposed model outperforms baseline models on the CREMA-D dataset and an extended dataset incorporating eNTERFACE’05, achieving significant performance improvements while reducing the number of parameters. These findings demonstrate the effectiveness of the proposed approach and provide a new technical solution for the field of emotion recognition. Full article
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28 pages, 9801 KiB  
Article
Large-Scale Monitoring of Potatoes Late Blight Using Multi-Source Time-Series Data and Google Earth Engine
by Zelong Chi, Hong Chen, Sheng Chang, Zhao-Liang Li, Lingling Ma, Tongle Hu, Kaipeng Xu and Zhenjie Zhao
Remote Sens. 2025, 17(6), 978; https://doi.org/10.3390/rs17060978 (registering DOI) - 11 Mar 2025
Abstract
Effective monitoring and management of potato late blight (PLB) is essential for sustainable agriculture. This study describes a methodology to improve PLB identification on a large scale. The method combines unsupervised and supervised machine learning algorithms. To improve the monitoring accuracy of the [...] Read more.
Effective monitoring and management of potato late blight (PLB) is essential for sustainable agriculture. This study describes a methodology to improve PLB identification on a large scale. The method combines unsupervised and supervised machine learning algorithms. To improve the monitoring accuracy of the PLB regression model, the study used the K-Means algorithm in conjunction with morphological operations to identify potato growth areas. Input data consisted of monthly NDVI from Sentinel-2 and VH bands from Sentinel-1 (covering the year 2021). The identification results were validated on 221 field survey samples with an F1 score of 0.95. To monitor disease severity, we compared seven machine learning models: CART decision trees (CART), Gradient Tree Boosting (GTB), Random Forest (RF), single optical data Random Forest Time series model (TS–RF), single radar data Random Forest Time series model (STS–RF), multi-source data Gradient Tree Boosting Time series model (MSTS–GTB), and multi-source data Random Forest Time series model (MSTS–RF). The MSTS–RF model was the best performer, with a validation RMSE of 20.50 and an R² of 0.71. The input data for the MSTS–RF model consisted of spectral indices (NDVI, NDWI, NDBI, etc.), radar features (VH-band and VV-band), texture features, and Sentinel-2 bands synthesized as a monthly time series from May to September 2021. The feature importance analysis highlights key features for disease identification: the NIR band (B8) for Sentinel-2, DVI, SAVI, and the VH band for Sentinel-1. Notably, the blue band data (458–523 nm) were critical during the month of May. These features are related to vegetation health and soil moisture are critical for early detection. This study presents for the first time a large-scale map of PLB distribution in China with an accuracy of 10 m and an RMSE of 26.52. The map provides valuable decision support for agricultural disease management, demonstrating the effectiveness and practical potential of the proposed method for large-scale monitoring. Full article
(This article belongs to the Special Issue Plant Disease Detection and Recognition Using Remotely Sensed Data)
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26 pages, 1223 KiB  
Systematic Review
Performance of Commercial Deep Learning-Based Auto-Segmentation Software for Prostate Cancer Radiation Therapy Planning: A Systematic Review
by Curtise K. C. Ng
Information 2025, 16(3), 215; https://doi.org/10.3390/info16030215 (registering DOI) - 11 Mar 2025
Abstract
As yet, there is no systematic review focusing on benefits and issues of commercial deep learning-based auto-segmentation (DLAS) software for prostate cancer (PCa) radiation therapy (RT) planning despite that NRG Oncology has underscored such necessity. This article’s purpose is to systematically review commercial [...] Read more.
As yet, there is no systematic review focusing on benefits and issues of commercial deep learning-based auto-segmentation (DLAS) software for prostate cancer (PCa) radiation therapy (RT) planning despite that NRG Oncology has underscored such necessity. This article’s purpose is to systematically review commercial DLAS software product performances for PCa RT planning and their associated evaluation methodology. A literature search was performed with the use of electronic databases on 7 November 2024. Thirty-two articles were included as per the selection criteria. They evaluated 12 products (Carina Medical LLC INTContour (Lexington, KY, USA), Elekta AB ADMIRE (Stockholm, Sweden), Limbus AI Inc. Contour (Regina, SK, Canada), Manteia Medical Technologies Co. AccuContour (Jian Sheng, China), MIM Software Inc. Contour ProtégéAI (Cleveland, OH, USA), Mirada Medical Ltd. DLCExpert (Oxford, UK), MVision.ai Contour+ (Helsinki, Finland), Radformation Inc. AutoContour (New York, NY, USA), RaySearch Laboratories AB RayStation (Stockholm, Sweden), Siemens Healthineers AG AI-Rad Companion Organs RT, syngo.via RT Image Suite and DirectORGANS (Erlangen, Germany), Therapanacea Annotate (Paris, France), and Varian Medical Systems, Inc. Ethos (Palo Alto, CA, USA)). Their results illustrate that the DLAS products can delineate 12 organs at risk (abdominopelvic cavity, anal canal, bladder, body, cauda equina, left (L) and right (R) femurs, L and R pelvis, L and R proximal femurs, and sacrum) and four clinical target volumes (prostate, lymph nodes, prostate bed, and seminal vesicle bed) with clinically acceptable outcomes, resulting in delineation time reduction, 5.7–81.1%. Although NRG Oncology has recommended each clinical centre to perform its own DLAS product evaluation prior to clinical implementation, such evaluation seems more important for AccuContour and Ethos due to the methodological issues of the respective single studies, e.g., small dataset used, etc. Full article
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16 pages, 1385 KiB  
Article
Development of a miRNA-Based Model for Lung Cancer Detection
by Kai Chin Poh, Toh Ming Ren, Goh Liuh Ling, John S Y Goh, Sarrah Rose, Alexa Wong, Sanhita S. Mehta, Amelia Goh, Pei-Yu Chong, Sim Wey Cheng, Samuel Sherng Young Wang, Seyed Ehsan Saffari, Darren Wan-Teck Lim and Na-Yu Chia
Cancers 2025, 17(6), 942; https://doi.org/10.3390/cancers17060942 (registering DOI) - 10 Mar 2025
Abstract
Background: Lung cancer is the leading cause of cancer-related mortality globally, with late-stage diagnoses contributing to poor survival rates. While lung cancer screening with low-dose computed tomography (LDCT) has proven effective in reducing mortality among heavy smokers, its limitations, including high false-positive rates [...] Read more.
Background: Lung cancer is the leading cause of cancer-related mortality globally, with late-stage diagnoses contributing to poor survival rates. While lung cancer screening with low-dose computed tomography (LDCT) has proven effective in reducing mortality among heavy smokers, its limitations, including high false-positive rates and resource intensiveness, restrict widespread use. Liquid biopsy, particularly using microRNA (miRNA) biomarkers, offers a promising adjunct to current screening strategies. This study aimed to evaluate the predictive power of a panel of serum miRNA biomarkers for lung cancer detection. Patients and Methods: A case-control study was conducted at two tertiary hospitals, enrolling 82 lung cancer cases and 123 controls. We performed an extensive literature review to shortlist 25 candidate miRNAs, of which 16 showed a significant two-fold increase in expression compared to the controls. Machine learning techniques, including Random Forest, K-Nearest Neighbors, Neural Networks, and Support Vector Machines, were employed to identify the top six miRNAs. We then evaluated predictive models, incorporating these biomarkers with lung nodule characteristics on LDCT. Results: A prediction model utilising six miRNA biomarkers (mir-196a, mir-1268, mir-130b, mir-1290, mir-106b and mir-1246) alone achieved area under the curve (AUC) values ranging from 0.78 to 0.86, with sensitivities of 70–78% and specificities of 73–85%. Incorporating lung nodule size significantly improved model performance, yielding AUC values between 0.96 and 0.99, with sensitivities of 92–98% and specificities of 93–98%. Conclusions: A prediction model combining serum miRNA biomarkers and nodule size showed high predictive power for lung cancer. Integration of the prediction model into current lung cancer screening protocols may improve patient outcomes. Full article
(This article belongs to the Special Issue Predictive Biomarkers for Lung Cancer)
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16 pages, 13302 KiB  
Article
Machine Learning-Based Partition Method for Cyclic Development Mode of Submarine Soil Martials from Offshore Wind Farms
by Ben He, Mingbao Lin, Zhishuai Zhang, Bo Han and Xinran Yu
J. Mar. Sci. Eng. 2025, 13(3), 533; https://doi.org/10.3390/jmse13030533 (registering DOI) - 10 Mar 2025
Abstract
Offshore wind turbines are subjected to long-term cyclic loads, and the seabed materials surrounding the foundation are susceptible to failure, which affects the safe construction and normal operation of offshore wind turbines. The existing studies of the cyclic mechanical properties of submarine soils [...] Read more.
Offshore wind turbines are subjected to long-term cyclic loads, and the seabed materials surrounding the foundation are susceptible to failure, which affects the safe construction and normal operation of offshore wind turbines. The existing studies of the cyclic mechanical properties of submarine soils focus on the accumulation strain and liquefaction, and few targeted studies are conducted on the hysteresis loop under cyclic loads. Therefore, 78 representative submarine soil samples from four offshore wind farms are tested in the study, and the cyclic behaviors under different confining pressures and CSR are investigated. The experiments reveal two unique development modes and specify the critical CSR of five submarine soil martials under different testing conductions. Based on the dynamic triaxial test results, the machine learning-based partition models for cyclic development mode were established, and the discrimination accuracy of the hysteresis loop were discussed. This study found that the RF model has a better generalization ability and higher accuracy than the GBDT model in discriminating the hysteresis loop of submarine soil, the RF model has achieved a prediction accuracy of 0.96 and a recall of 0.95 on the test dataset, which provides an important theoretical basis and technical support for the design and construction of offshore wind turbines. Full article
(This article belongs to the Section Ocean Engineering)
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16 pages, 2444 KiB  
Article
Enhanced Privacy-Preserving Architecture for Fundus Disease Diagnosis with Federated Learning
by Raymond Jiang, Yulia Kumar and Dov Kruger
Appl. Sci. 2025, 15(6), 3004; https://doi.org/10.3390/app15063004 (registering DOI) - 10 Mar 2025
Abstract
In recent years, advances in diagnosing and classifying diseases using machine learning (ML) have grown exponentially. However, due to the many privacy regulations regarding personal data, pooling together data from multiple sources and storing them in a single (centralized) location for traditional ML [...] Read more.
In recent years, advances in diagnosing and classifying diseases using machine learning (ML) have grown exponentially. However, due to the many privacy regulations regarding personal data, pooling together data from multiple sources and storing them in a single (centralized) location for traditional ML model training are often infeasible. Federated learning (FL), a collaborative learning paradigm, can sidestep this major pitfall by creating a global ML model that is trained by aggregating model weights from individual models that are separately trained on their own data silos, therefore avoiding most data privacy concerns. This study addresses the centralized data issue with FL by applying a novel DataWeightedFed architectural approach for effective fundus disease diagnosis from ophthalmic images. It includes a novel method for aggregating model weights by comparing the size of each model’s data and taking a dynamically weighted average of all the model’s weights. Experimental results showed a small average 1.85% loss in accuracy when training using FL compared to centralized ML model systems, a nearly 92% improvement over the conventional 55% accuracy loss. The obtained results demonstrate that this study’s FL architecture can maximize both privacy preservation and accuracy for ML in fundus disease diagnosis and provide a secure, collaborative ML model training solution within the eye healthcare space. Full article
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23 pages, 8242 KiB  
Article
Study of Factors Influencing Thermal Comfort at Tram Stations in Guangzhou Based on Machine Learning
by Xin Chen, Huanchen Zhao, Beini Wang and Bo Xia
Buildings 2025, 15(6), 865; https://doi.org/10.3390/buildings15060865 (registering DOI) - 10 Mar 2025
Abstract
As global climate change intensifies, the frequency and severity of extreme weather events continue to rise. However, research on semi-outdoor and transitional spaces remains limited, and transportation stations are typically not fully enclosed. Therefore, it is crucial to gain a deeper understanding of [...] Read more.
As global climate change intensifies, the frequency and severity of extreme weather events continue to rise. However, research on semi-outdoor and transitional spaces remains limited, and transportation stations are typically not fully enclosed. Therefore, it is crucial to gain a deeper understanding of the environmental needs of users in these spaces. This study employs machine learning (ML) algorithms and the SHAP (SHapley Additive exPlanations) methodology to identify and rank the critical factors influencing outdoor thermal comfort at tram stations. We collected microclimatic data from tram stations in Guangzhou, along with passenger comfort feedback, to construct a comprehensive dataset encompassing environmental parameters, individual perceptions, and design characteristics. A variety of ML models, including Extreme Gradient Boosting (XGB), Light Gradient Boosting Machine (LightGBM), Categorical Boosting (CatBoost), Random Forest (RF), and K-Nearest Neighbors (KNNs), were trained and validated, with SHAP analysis facilitating the ranking of significant factors. The results indicate that the LightGBM and CatBoost models performed exceptionally well, identifying key determinants such as relative humidity (RH), outdoor air temperature (Ta), mean radiant temperature (Tmrt), clothing insulation (Clo), gender, age, body mass index (BMI), and the location of the space occupied in the past 20 min prior to waiting (SOP20). Notably, the significance of physical parameters surpassed that of physiological and behavioral factors. This research provides clear strategic guidance for urban planners, public transport managers, and designers to enhance thermal comfort at tram stations while offering a data-driven approach to optimizing outdoor spaces and promoting sustainable urban development. Full article
(This article belongs to the Section Building Energy, Physics, Environment, and Systems)
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20 pages, 5974 KiB  
Article
Improving the Accuracy of Tree Species Mapping by Sentinel-2 Images Using Auxiliary Data—A Case Study of Slyudyanskoye Forestry Area near Lake Baikal
by Anastasia Popova
Forests 2025, 16(3), 487; https://doi.org/10.3390/f16030487 (registering DOI) - 10 Mar 2025
Abstract
Timely and accurate information on forest composition is crucial for ecosystem conservation and management tasks. Information regarding the distribution and extent of forested areas can be derived through the classification of satellite imagery. However, optical data alone are often insufficient to achieve the [...] Read more.
Timely and accurate information on forest composition is crucial for ecosystem conservation and management tasks. Information regarding the distribution and extent of forested areas can be derived through the classification of satellite imagery. However, optical data alone are often insufficient to achieve the required accuracy due to the similarity in spectral characteristics among tree species, particularly in mountainous regions. One approach to improving the accuracy of forest classification is the integration of auxiliary environmental data. This paper presents the results of research conducted in the Slyudyanskoye Forestry area in the Irkutsk Region. A dataset comprising 101 variables was collected, including Sentinel-2 bands, vegetation indices, and climatic, soil, and topographic data, as well as forest canopy height. The classification was performed using the Random Forest machine learning method. The results demonstrated that auxiliary environmental data significantly improved the performance of the tree species classification model, with the overall accuracy increasing from 49.59% (using only Sentinel-2 bands) to 80.69% (combining spectral data with auxiliary variables). The most significant improvement in accuracy was achieved through the incorporation of climatic and soil features. The most important variables were the shortwave infrared band B11, forest canopy height, the length of the growing season, and the number of days with snow cover. Full article
(This article belongs to the Section Forest Inventory, Modeling and Remote Sensing)
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29 pages, 1828 KiB  
Review
Advances in Fermentation Technology: A Focus on Health and Safety
by Theoneste Niyigaba, Kübra Küçükgöz, Danuta Kołożyn-Krajewska, Tomasz Królikowski and Monika Trząskowska
Appl. Sci. 2025, 15(6), 3001; https://doi.org/10.3390/app15063001 - 10 Mar 2025
Abstract
Fermentation represents a pivotal bioconversion process that enhances foodstuffs’ nutritional and sensory attributes while playing a crucial role in global food systems. Nevertheless, concerns about safety issues associated with microbial contamination and the production of biogenic amines are often understated. This review appraised [...] Read more.
Fermentation represents a pivotal bioconversion process that enhances foodstuffs’ nutritional and sensory attributes while playing a crucial role in global food systems. Nevertheless, concerns about safety issues associated with microbial contamination and the production of biogenic amines are often understated. This review appraised recent advancements in fermentation technology, emphasising their association with the health and safety of fermented foods. Key advances include predictive microbiology models, in some cases achieving up to 95% accuracy in predicting microbial behaviour, and high-throughput sequencing (HTS) for microbial enrichment. In addition, advanced detection methods such as biosensors and PCR-based assays enable the rapid identification of contaminants, improving manufacturing processes and preserving product integrity. Advanced bioreactor technologies equipped with real-time monitoring systems have been shown to increase fermentation efficiency. Moreover, innovative packaging, artificial intelligence, machine learning models, and sensor technologies have optimised fermentation processes and contributed to tracking quality and safety in the blockchain technology supply chain, potentially reducing spoilage rates and showing a decrease in production times. This study also addresses regulatory frameworks essential for establishing robust safety protocols. Integrating advanced fermentation technologies is imperative to meet the growing global demand for safe fermented foods. Continuous research and innovation are needed to address safety challenges and promote industry practices prioritising health and quality, ensuring public safety and building consumer confidence in fermented products. Full article
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27 pages, 14625 KiB  
Article
Generative Architectural Design from Textual Prompts: Enhancing High-Rise Building Concepts for Assisting Architects
by Feng Yang and Wenliang Qian
Appl. Sci. 2025, 15(6), 3000; https://doi.org/10.3390/app15063000 - 10 Mar 2025
Abstract
In the early stages of architectural design, architects convert initial ideas into concrete design schemes, which heavily rely on their creativity and consume considerable time. Therefore, generative design methods based on artificial intelligence are promising for such tasks. However, effectively communicating design concepts [...] Read more.
In the early stages of architectural design, architects convert initial ideas into concrete design schemes, which heavily rely on their creativity and consume considerable time. Therefore, generative design methods based on artificial intelligence are promising for such tasks. However, effectively communicating design concepts to machines is challenging. To address this challenge, this paper proposes a novel cross-model approach for architectural design concepts using textual descriptions to assist architects, comprising a design concept extraction module and an architectural appearance generation module. The design concept extraction module adopts a contrastive learning framework to yield a text encoder with semantic extraction. Subsequently, the architectural appearance generation module proposes a novel deep sparse and text fusion generative adversarial network to convert the extracted design concept semantics into conceptual sketches, utilizing the unique sparsity of sketches. Additionally, it employs the pre-trained latent stable diffusion model to generate realistic and diverse high-rise building renderings, simulating the recreation processes of architects. The generated designs are evaluated qualitatively and quantitatively and further compared with existing real-life buildings to demonstrate the effectiveness of the proposed method. Furthermore, we demonstrate the feasibility of applying the proposed methodology in the early stages of architectural design by modeling a generated design. Full article
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26 pages, 682 KiB  
Review
The Development of a River Quality Prediction Model That Is Based on the Water Quality Index via Machine Learning: A Review
by Hassan Shaheed, Mohd Hafiz Zawawi and Gasim Hayder
Processes 2025, 13(3), 810; https://doi.org/10.3390/pr13030810 - 10 Mar 2025
Abstract
This review, “The Development of a River Quality Prediction Model That Is Based on the Water Quality Index using Machine Learning: A Review”, discusses and evaluates research articles and attempts to incorporate ML algorithms into the water quality index (WQI) to improve the [...] Read more.
This review, “The Development of a River Quality Prediction Model That Is Based on the Water Quality Index using Machine Learning: A Review”, discusses and evaluates research articles and attempts to incorporate ML algorithms into the water quality index (WQI) to improve the prediction of river water quality. This original study confirms how new methodologies like LSTM, CNNs, and random forest perform better than previous methods, as they offer real-time predictions, operational cost saving, and opportunities for handling big data. This review finds that, in addition to good case studies and real-life applications, there is a need to expand in the following areas: impacts of climate change, ways of enhancing data representation, and concerns to do with ethics as well as data privacy. Furthermore, this review outlines issues, such as data scarcity, model explainability, and computational overhead in real-world ML applications, as well as strategies to preemptively address these issues in order to improve the versatility of data-driven models in various domains. Moving to the analysis of the review specifically to discuss the propositions, the identified key points focus on the use of complex approaches and interdisciplinarity and the involvement of stakeholders. Due to the added specificity and depth in a number of comparisons and specific technical and policy discussions, this sweeping review offers a broad view of how to proceed in enhancing the usefulness of the predictive technologies that will be central to environmental forecasting. Full article
(This article belongs to the Section Advanced Digital and Other Processes)
49 pages, 3487 KiB  
Review
Exosomes in Precision Oncology and Beyond: From Bench to Bedside in Diagnostics and Therapeutics
by Emile Youssef, Dannelle Palmer, Brandon Fletcher and Renee Vaughn
Cancers 2025, 17(6), 940; https://doi.org/10.3390/cancers17060940 - 10 Mar 2025
Abstract
Exosomes have emerged as pivotal players in precision oncology, offering innovative solutions to longstanding challenges such as metastasis, therapeutic resistance, and immune evasion. These nanoscale extracellular vesicles facilitate intercellular communication by transferring bioactive molecules that mirror the biological state of their parent cells, [...] Read more.
Exosomes have emerged as pivotal players in precision oncology, offering innovative solutions to longstanding challenges such as metastasis, therapeutic resistance, and immune evasion. These nanoscale extracellular vesicles facilitate intercellular communication by transferring bioactive molecules that mirror the biological state of their parent cells, positioning them as transformative tools for cancer diagnostics and therapeutics. Recent advancements in exosome engineering, artificial intelligence (AI)-driven analytics, and isolation technologies are breaking barriers in scalability, reproducibility, and clinical application. Bioengineered exosomes are being leveraged for CRISPR-Cas9 delivery, while AI models are enhancing biomarker discovery and liquid biopsy accuracy. Despite these advancements, key obstacles such as heterogeneity in exosome populations and the lack of standardized isolation protocols persist. This review synthesizes pioneering research on exosome biology, molecular engineering, and clinical translation, emphasizing their dual roles as both mediators of tumor progression and tools for intervention. It also explores emerging areas, including microbiome–exosome interactions and the integration of machine learning in exosome-based precision medicine. By bridging innovation with translational strategies, this work charts a forward-looking path for integrating exosomes into next-generation cancer care, setting it apart as a comprehensive guide to overcoming clinical and technological hurdles in this rapidly evolving field. Full article
(This article belongs to the Section Cancer Causes, Screening and Diagnosis)
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21 pages, 1239 KiB  
Review
Advancing Stable Isotope Analysis for Alcoholic Beverages’ Authenticity: Novel Approaches in Fraud Detection and Traceability
by Yiqian Ma, Yalan Li, Feilong Shao, Yuanyu Lu, Wangni Meng, Karyne M. Rogers, Di Sun, Hao Wu and Xiaodong Peng
Foods 2025, 14(6), 943; https://doi.org/10.3390/foods14060943 - 10 Mar 2025
Abstract
Background: Alcoholic beverages have been popular for thousands of years due to their unique flavors and cultural significance. However, the industry’s high profit margins have led to increasingly sophisticated counterfeiting practices. Stable isotope analysis has emerged as one of the most promising techniques [...] Read more.
Background: Alcoholic beverages have been popular for thousands of years due to their unique flavors and cultural significance. However, the industry’s high profit margins have led to increasingly sophisticated counterfeiting practices. Stable isotope analysis has emerged as one of the most promising techniques for addressing authenticity and traceability challenges in alcoholic beverages. Scope and approach: This review presents a comprehensive summary of the principles and recent advancements in the application of stable isotope techniques for authenticity assessment. It examines their use in detecting fraud (e.g., identifying edible alcohol, exogenous water, carbonylation, and trace compounds), vintage identification, and geographical origin determination across various alcoholic beverages, with a particular focus on wine, Chinese baijiu, and beer. Conclusions: Stable isotope analysis is a powerful tool for verifying the authenticity of alcoholic beverages, offering effective solutions to combat counterfeiting, mislabeling, and adulteration. Future studies should focus on understanding the ecological, biological, and hydrometeorological factors influencing isotope signatures and develop advanced multi-isotope and chemometric approaches to improve reliability. Expanding global databases and integrating emerging technologies such as artificial intelligence (AI) and machine learning will further enhance the effectiveness and accessibility of stable isotope techniques, ensuring safer and higher-quality alcoholic beverages for consumers worldwide. Full article
(This article belongs to the Section Drinks and Liquid Nutrition)
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25 pages, 5064 KiB  
Article
Drivers of Structural and Functional Resilience Following Extreme Fires in Boreal Forests of Northeast China
by Jianyu Yao, Xiaoyang Kong, Lei Fang, Zhaohan Huo, Yanbo Peng, Zile Han, Shilong Ren, Jinyue Chen, Xinfeng Wang and Qiao Wang
Fire 2025, 8(3), 108; https://doi.org/10.3390/fire8030108 - 10 Mar 2025
Abstract
Ongoing climate change has intensified fire disturbances in boreal forests globally, posing significant risks to forest ecosystem structure and function, with the potential to trigger major regime shifts. Understanding how environmental factors regulate the resilience of key structural and functional parameters is critical [...] Read more.
Ongoing climate change has intensified fire disturbances in boreal forests globally, posing significant risks to forest ecosystem structure and function, with the potential to trigger major regime shifts. Understanding how environmental factors regulate the resilience of key structural and functional parameters is critical for sustaining and enhancing ecosystem services under global change. This study analyzed the resilience of forest ecosystems following three representative extreme fires in the Greater Xing’an Mountains (GXM) via the temporal evolution of the leaf area index (LAI), net primary productivity (NPP), and evapotranspiration (ET) as key indicators. A comprehensive wall-to-wall assessment was conducted, integrating gradient boosting machine (GBM) modeling with Shapley Additive Explanation (SHAP) to identify the dominant factors influencing postfire resilience. The results revealed that NPP demonstrated stronger resilience than ET and LAI, suggesting the prioritization of functional restoration over structural recovery in the postfire landscape of the GXM. The GBM-SHAP model explained 45% to 69% of the variance in the resilience patterns of the three parameters. Among the regulatory factors, extreme precipitation and temperature during the growing season were found to exert more significant influences on resilience than landscape-scale factors, such as burn severity, topography, and prefire vegetation composition. The spatial asynchrony in resilience patterns between structural and functional parameters highlighted the complex interplay of climatic drivers and ecological processes during post-disturbance recovery. Our study emphasized the importance of prioritizing functional restoration in the short term to support ecosystem recovery processes and services. Despite the potential limitations imposed by the coarse spatial granularity of the input data, our findings provide valuable insights for postfire management strategies, enabling the effective allocation of resources to increase ecosystem resilience and facilitating long-term adaptation to changing fire regimes. Full article
(This article belongs to the Special Issue Effects of Climate Change on Fire Danger)
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