Genetic Resources and Gene Editing Tools for Plant Resistance: Recent Advancements and Future Perspectives

A special issue of Biology (ISSN 2079-7737). This special issue belongs to the section "Plant Science".

Deadline for manuscript submissions: 31 July 2024 | Viewed by 2075

Special Issue Editor


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Guest Editor
1. Department of Bioenvironmental Chemistry, Jeonbuk National University, Jeonju 54896, Republic of Korea
2. Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju 54896, Republic of Korea
Interests: bacterial metal reduction; bacterial nitrogen cycle in soil; rhizosphere microbiology; plant–bacteria interactions; microbiome analysis

Special Issue Information

Dear Colleagues,

Agricultural production faces global challenges due to climate change, insufficient arable land, population growth, and abiotic and biotic stresses. Traditional breeding approaches have been widely used to mitigate the risks of abiotic and biotic stresses. The availability of genome sequence data for numerous crop plants and precise genome editing tools have revolutionized plant breeding programs. The discovery of gene editing technology to manipulate crop stress-responsive genes and associated molecular networks has paved the way for sustainable crop management. Genome editing tools enable for desired changes in an organism's DNA by introducing an insertion/deletion (indel) and mutation into the sequences of particular genes via recruiting specific nucleases such as zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), or the clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system. CRISPR technology has revolutionized life science research and has achieved remarkable results in resistance breeding for important cereals, vegetables, and fruit crops. Integrated approaches based on genomics, transcriptomics, proteomics, and metabolomics are now further elucidating the molecular mechanisms underlying stress responses. However, in recent years, significant advances in modern computational technology, like cryo–electron microscopy and Alphafold, predict the three-dimensional structures of proteins with higher accuracy and provide a molecular framework for studying proposed resistance mechanisms in plants. These cutting-edge technologies may generate broad-spectrum resistance against biotic and abiotic stress in crop plants. Hence, the main aim of the proposed Special Issue is to gather research ideas, findings, innovations, and knowledge in the research areas of genomics and plant resistance.

We invite researchers to contribute their original research papers, methods, perspectives, opinions, and reviews on the following themes.

Potential topics include, but are not limited to:

  • Abiotic and biotic stresses;
  • CRISPR/Cas-mediated genome editing for plant resistance;
  • Genome editing for targeted improvement;
  • Genome engineering and crop improvement;
  • Protein engineering to elucidate the mechanism of environmental stress;
  • Resistance mechanisms of plant disease management;
  • Role of plant NLR protein in plant immunity;
  • Transcriptional regulation of stress-inducible genes;
  • Transgene-free genome editing for plant resistance.

Dr. Ji-Hoon Lee
Guest Editor

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Keywords

  • abiotic stress
  • biotic stress
  • crop improvement
  • genome editing
  • plant resistance

Published Papers (2 papers)

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Research

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16 pages, 3398 KiB  
Article
Cloning and Functional Analysis of CsROP5 and CsROP10 Genes Involved in Cucumber Resistance to Corynespora cassiicola
by Guangchao Yu, Lian Jia, Ning Yu, Miao Feng and Yue Qu
Biology 2024, 13(5), 308; https://doi.org/10.3390/biology13050308 - 28 Apr 2024
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Abstract
The cloning of resistance-related genes CsROP5/CsROP10 and the analysis of their mechanism of action provide a theoretical basis for the development of molecular breeding of disease-resistant cucumbers. The structure domains of two Rho-related guanosine triphosphatases from plant (ROP) genes were systematically [...] Read more.
The cloning of resistance-related genes CsROP5/CsROP10 and the analysis of their mechanism of action provide a theoretical basis for the development of molecular breeding of disease-resistant cucumbers. The structure domains of two Rho-related guanosine triphosphatases from plant (ROP) genes were systematically analyzed using the bioinformatics method in cucumber plants, and the genes CsROP5 (Cucsa.322750) and CsROP10 (Cucsa.197080) were cloned. The functions of the two genes were analyzed using reverse-transcription quantitative PCR (RT-qPCR), virus-induced gene silencing (VIGS), transient overexpression, cucumber genetic transformation, and histochemical staining technology. The conserved elements of the CsROP5/CsROP10 proteins include five sequence motifs (G1-G5), a recognition site for serine/threonine kinases, and a hypervariable region (HVR). The knockdown of CsROP10 through VIGS affected the transcript levels of ABA-signaling-pathway-related genes (CsPYL, CsPP2Cs, CsSnRK2s, and CsABI5), ROS-signaling-pathway-related genes (CsRBOHD and CsRBOHF), and defense-related genes (CsPR2 and CsPR3), thereby improving cucumber resistance to Corynespora cassiicola. Meanwhile, inhibiting the expression of CsROP5 regulated the expression levels of ROS-signaling-pathway-related genes (CsRBOHD and CsRBOHF) and defense-related genes (CsPR2 and CsPR3), thereby enhancing the resistance of cucumber to C. cassiicola. Overall, CsROP5 and CsROP10 may participate in cucumber resistance to C. cassiicola through the ROS and ABA signaling pathways. Full article
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Review

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27 pages, 1867 KiB  
Review
Genetic Databases and Gene Editing Tools for Enhancing Crop Resistance against Abiotic Stress
by Alpana Joshi, Seo-Yeon Yang, Hyung-Geun Song, Jiho Min and Ji-Hoon Lee
Biology 2023, 12(11), 1400; https://doi.org/10.3390/biology12111400 - 3 Nov 2023
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Abstract
Abiotic stresses extensively reduce agricultural crop production globally. Traditional breeding technology has been the fundamental approach used to cope with abiotic stresses. The development of gene editing technology for modifying genes responsible for the stresses and the related genetic networks has established the [...] Read more.
Abiotic stresses extensively reduce agricultural crop production globally. Traditional breeding technology has been the fundamental approach used to cope with abiotic stresses. The development of gene editing technology for modifying genes responsible for the stresses and the related genetic networks has established the foundation for sustainable agriculture against environmental stress. Integrated approaches based on functional genomics and transcriptomics are now expanding the opportunities to elucidate the molecular mechanisms underlying abiotic stress responses. This review summarizes some of the features and weblinks of plant genome databases related to abiotic stress genes utilized for improving crops. The gene-editing tool based on clustered, regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) has revolutionized stress tolerance research due to its simplicity, versatility, adaptability, flexibility, and broader applications. However, off-target and low cleavage efficiency hinder the successful application of CRISPR/Cas systems. Computational tools have been developed for designing highly competent gRNA with better cleavage efficiency. This powerful genome editing tool offers tremendous crop improvement opportunities, overcoming conventional breeding techniques’ shortcomings. Furthermore, we also discuss the mechanistic insights of the CRISPR/Cas9-based genome editing technology. This review focused on the current advances in understanding plant species’ abiotic stress response mechanism and applying the CRISPR/Cas system genome editing technology to develop crop resilience against drought, salinity, temperature, heavy metals, and herbicides. Full article
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