Increased CCL-5 (RANTES) Gene Expression in the Choroid Plexus of Dogs with Canine Leishmaniosis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Sample Collection
2.2. Histopathological Evaluation of the Brain
2.3. Detection and Quantification of Leishmania DNA in the Spleen and CP
2.4. Cytokines and Chemokines Gene Expression in the CP
2.5. Cytokines in the Serum and CSF
2.6. Albumin and Anti-Leishmania Antibodies in the CSF
2.7. Statistical Analysis
3. Results
3.1. Clinical Staging
3.2. Brain Histopathology and Immunohistochemistry
3.3. Detection and Quantification of Leishmania DNA in the CP
3.4. Gene Expression of Cytokines and Chemokines in the CP
3.5. Cytokines in the Serum and CSF
3.6. Albumin and Anti-Leishmania Antibodies in the CSF
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
References
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Intensity of Inflammation | Brain Region | ||
---|---|---|---|
Leptomeninges (%) | Subependyma (%) | Choroid Plexus (%) | |
0 (−) | 0.0 | 42.1 | 5.3 |
1 (+) | 21.1 | 36.8 | 15.8 |
2 (++) | 36.8 | 21.1 | 47.4 |
3 (+++) | 42.1 | 0.0 | 31.6 |
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Silva, J.E.d.S.; Jussiani, G.G.; Grano, F.G.; Pelissari, M.C.C.; de Melo, G.D.; Negrão Watanabe, T.T.; de Lima, V.F.; Machado, G.F. Increased CCL-5 (RANTES) Gene Expression in the Choroid Plexus of Dogs with Canine Leishmaniosis. Animals 2023, 13, 2060. https://doi.org/10.3390/ani13132060
Silva JEdS, Jussiani GG, Grano FG, Pelissari MCC, de Melo GD, Negrão Watanabe TT, de Lima VF, Machado GF. Increased CCL-5 (RANTES) Gene Expression in the Choroid Plexus of Dogs with Canine Leishmaniosis. Animals. 2023; 13(13):2060. https://doi.org/10.3390/ani13132060
Chicago/Turabian StyleSilva, José Eduardo dos Santos, Giulia Gonçalves Jussiani, Fernanda Grecco Grano, Maria Cecília Clarindo Pelissari, Guilherme Dias de Melo, Tatiane Terumi Negrão Watanabe, Valéria Felix de Lima, and Gisele Fabrino Machado. 2023. "Increased CCL-5 (RANTES) Gene Expression in the Choroid Plexus of Dogs with Canine Leishmaniosis" Animals 13, no. 13: 2060. https://doi.org/10.3390/ani13132060