Characterization of Hydrocortisone Biometabolites and 18S rRNA Gene in Chlamydomonas reinhardtii Cultures
Abstract
:Introduction
Results and Discussion
Identification of the algal strain
Partial sequence of the 18S rRNA
5′attgatcaagaacgaaagttgggggctcgaagacgattagataccgtcgtagtctcaaccataaacgatgccgactagggatt ggcagatgttcttttgatgactctgccagcaccttatgagaaatcaaagtttttgggttccggggggagtatggtcgcaaggctgaa acttaaaggaattgacggaagggcaccaccaggcgtggagcctgcggcttaatttgactcaacacggggaaacttaccaggtc cagacacgggaaggattgacagattgagagctctttcttgattctgtgggtggtggtgcatggccgttcttagttggtgggttgcctt gtcaggttgattccggtaacgaacgagacctcagcctgctaaatagtcagcatcgcacctgcggtgcgccgacttcttagaggg actattggcgtttagccaatggaagtatgaggcgataacaggtctgtgatgcccttagatgttctgggccgcacgcgcgctacact gacgcgaccaacgagcctatccttggccgagaggcccgggtaatc 3′
Biotransformation of hydrocortisone (1)
Product characterization
Conclusions
Experimental
General
Chemicals
Collection, preservation and identification of the alga
Incubation conditions
Time course study and the effect of substrate concentration
Products isolation and analysis
18S ribosomal RNA sequencing
Acknowledgements
References
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Ghasemi, Y.; Rasoul-Amini, S.; Morowvat, M.H.; Raee, M.J.; Ghoshoon, M.B.; Nouri, F.; Negintaji, N.; Parvizi, R.; Mosavi-Azam, S.B. Characterization of Hydrocortisone Biometabolites and 18S rRNA Gene in Chlamydomonas reinhardtii Cultures. Molecules 2008, 13, 2416-2425. https://doi.org/10.3390/molecules13102416
Ghasemi Y, Rasoul-Amini S, Morowvat MH, Raee MJ, Ghoshoon MB, Nouri F, Negintaji N, Parvizi R, Mosavi-Azam SB. Characterization of Hydrocortisone Biometabolites and 18S rRNA Gene in Chlamydomonas reinhardtii Cultures. Molecules. 2008; 13(10):2416-2425. https://doi.org/10.3390/molecules13102416
Chicago/Turabian StyleGhasemi, Younes, Sara Rasoul-Amini, Mohammad Hossein Morowvat, Mohammad Javad Raee, Mohammad Bagher Ghoshoon, Fatemeh Nouri, Narges Negintaji, Rezvan Parvizi, and Seyed Bagher Mosavi-Azam. 2008. "Characterization of Hydrocortisone Biometabolites and 18S rRNA Gene in Chlamydomonas reinhardtii Cultures" Molecules 13, no. 10: 2416-2425. https://doi.org/10.3390/molecules13102416