Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts
Abstract
:1. Introduction
2. Results and Discussion
2.1. Results
2.1.1. The Extraction Yield and Quantation of IPE
2.1.2. The Physical Characteristics of IPE
2.1.3. Measurement of Reducing Power
2.1.4. DPPH Radical Scavenging Activity of IPE and IPH
2.1.5. Metal Chelating Activity
2.1.6. Determination of Hydroxyl Radical Scavenging Activity
2.1.7. Peroxide Scavenging Assay
2.1.8. The Inhibitory Effects of IPE and IPH on AAPH-Induced Erythrocyte Hemolysis
2.1.9. Fluorescence Assay of Intracellular ROS
2.2. Discussion
3. Materials and Methods
3.1. Materials
3.2. Preparation of Ixora Parviflora Extract (IPE) and Its Hydrolysates (IPH)
3.3. Quantation of IPE
3.3.1. Total Phenolic Content of IPE
3.3.2. Total Flavonoids Content of IPE
3.3.3. Quantation of IPE by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) Method
3.4. Physical Characteristics of IPE
3.4.1. pH Value
3.4.2. Absorption Spectrum of IPE
3.5. Measurement of Reducing Power
3.6. DPPH Radical Scavenging Activity
3.7. Metal Chelating Activity
3.8. Determination of Hydroxyl Radical Scavenging Activity
3.9. Peroxide Scavenging Assay
3.10. AAPH-Induced Hemolysis Assay
3.11. Fluorescence Assay of Intracellular ROS
3.12. Statistical Analysis
4. Conclusions
Contributions
Conflict of interest
Acknowledgments
References
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Wen, K.-C.; Chiu, H.-H.; Fan, P.-C.; Chen, C.-W.; Wu, S.-M.; Chang, J.-H.; Chiang, H.-M. Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts. Molecules 2011, 16, 5735-5752. https://doi.org/10.3390/molecules16075735
Wen K-C, Chiu H-H, Fan P-C, Chen C-W, Wu S-M, Chang J-H, Chiang H-M. Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts. Molecules. 2011; 16(7):5735-5752. https://doi.org/10.3390/molecules16075735
Chicago/Turabian StyleWen, Kuo-Ching, Hua-Hsien Chiu, Pei-Ching Fan, Chien-Wen Chen, Shih-Mei Wu, Jung-Hsiang Chang, and Hsiu-Mei Chiang. 2011. "Antioxidant Activity of Ixora parviflora in a Cell/Cell-Free System and in UV-Exposed Human Fibroblasts" Molecules 16, no. 7: 5735-5752. https://doi.org/10.3390/molecules16075735