Effects of Combined Elicitors on Tanshinone Metabolic Profiling and SmCPS Expression in Salvia miltiorrhiza Hairy Root Cultures
Abstract
:1. Introduction
2. Results and Discussion
2.1. Establishment of Salvia miltiorrhiza Hairy Root Cultures
2.2. Effects of Combined Elicitors on SmCPS Expression
2.3. Tanshinone Production in S. miltiorrhiza Hairy Root Cultures
2.4. Effects of Combined Elicitors on the Accumulation of Cryptotanshinone and Dihydrotanshinone I
3. Experimental
3.1. Plant Materials
3.2. Growth of Agrobacterium rhizogenes and Hairy Root Cultures
3.3. Elicitation of Combined Elicitors
3.4. Metabolite Analysis of Hairy Roots
3.5. Quantitative Real-Time PCR
gene | Sequences of the primers(5′→3′) |
---|---|
SmCPS | GAGGGAGAGGTGAGGAAGGAA |
AGGGAACAAAAGTTGAAAAGG | |
β-Actin | AGGAACCACCGATCCAGACA |
GGTGCCCTGAGGTCCTGTT |
3.6. Statistical Analysis
4. Conclusions
Acknowledgments
Conflicts of Interest
References
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Cheng, Q.; He, Y.; Li, G.; Liu, Y.; Gao, W.; Huang, L. Effects of Combined Elicitors on Tanshinone Metabolic Profiling and SmCPS Expression in Salvia miltiorrhiza Hairy Root Cultures. Molecules 2013, 18, 7473-7485. https://doi.org/10.3390/molecules18077473
Cheng Q, He Y, Li G, Liu Y, Gao W, Huang L. Effects of Combined Elicitors on Tanshinone Metabolic Profiling and SmCPS Expression in Salvia miltiorrhiza Hairy Root Cultures. Molecules. 2013; 18(7):7473-7485. https://doi.org/10.3390/molecules18077473
Chicago/Turabian StyleCheng, Qiqing, Yunfei He, Geng Li, Yujia Liu, Wei Gao, and Luqi Huang. 2013. "Effects of Combined Elicitors on Tanshinone Metabolic Profiling and SmCPS Expression in Salvia miltiorrhiza Hairy Root Cultures" Molecules 18, no. 7: 7473-7485. https://doi.org/10.3390/molecules18077473