Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery
Abstract
:1. Introduction
2. Results and Discussion
2.1. SMM Prescreening
2.2. In Vivo Microscopy Studies
ID | Structure, Localization, Color | ID | Structure, Localization, Color |
---|---|---|---|
B5 | Perinuclear/cytoplasm (blue) | D10 | Mitochondria (red) |
C4 | Lipid droplets (blue) | E5 | Mitochondria (red) |
C6 | Lipid droplets (blue) | B11 | Plasma membrane (red) |
B3 | Lipid droplets (blue) | C2 | Mitochondria/nucleolus convertible(far-red) |
B2 | Lipid droplets (blue-green) | C3 | Mitochondria (far-red) |
B4 | Lipid droplets (blue-green) | DMSO | Solvent used as control (colorless) |
3. Experimental Section
3.1. Materials
3.2. Small Molecule Microarray (SMM)
3.2.1. Preparation
3.2.2. Signal Detection and Data Analysis
3.3. Cell Culturing
3.4. Cell treatment
3.4.1. Treatment with Selected Compounds
3.4.2. Co-Labeling
3.5. Confocal Laser Scanning Microscopy (CLSM)
4. Conclusions
Acknowledgments
Conflicts of Interest
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Molnár, E.; Kuntam, S.; Cingaram, P.K.R.; Peksel, B.; Suresh, B.; Fábián, G.; Fehér, L.Z.; Bokros, A.; Medgyesi, Á.; Ayaydin, F.; et al. Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery. Molecules 2013, 18, 9999-10013. https://doi.org/10.3390/molecules18089999
Molnár E, Kuntam S, Cingaram PKR, Peksel B, Suresh B, Fábián G, Fehér LZ, Bokros A, Medgyesi Á, Ayaydin F, et al. Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery. Molecules. 2013; 18(8):9999-10013. https://doi.org/10.3390/molecules18089999
Chicago/Turabian StyleMolnár, Eszter, Soujanya Kuntam, Pradeep Kumar Reddy Cingaram, Begüm Peksel, Bhavyashree Suresh, Gabriella Fábián, Liliána Z. Fehér, Attila Bokros, Ágnes Medgyesi, Ferhan Ayaydin, and et al. 2013. "Combination of Small Molecule Microarray and Confocal Microscopy Techniques for Live Cell Staining Fluorescent Dye Discovery" Molecules 18, no. 8: 9999-10013. https://doi.org/10.3390/molecules18089999