On-Line Screening, Isolation and Identification of Antioxidant Compounds of Helianthemum ruficomum
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Structure Elucidation of Compounds
2.2. Identification of Chromatographic Peaks and Antioxidant Activity of Plant Extracts and Pure Compounds
3. Materials and Methods
3.1. General Procedure
3.2. Plant Material
3.3. Extraction and Isolation
3.4. Solvents and Chemicals
3.5. On-Line HPLC-ABTS•+ Assay
3.6. Oxygen Radical Absorbance Capacity (ORAC)
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Peaks | Compounds | ABTS (µgTE/mL) | Radical-Scavenging Activity (mAU) | Scavenging Activity Percent (%) |
---|---|---|---|---|
A | Not identified | 131.83 | 546 | 17.40 |
B | Not identified | 137.47 | 569 | 16.86 |
1 | Protocatechuic acid | 81.56 | 341 | 8.37 |
C | Not identified | 63.96 | 269 | 5.48 |
D | Not identified | 42.57 | 181 | 3.97 |
E | Not identified | 118.05 | 490 | 18.50 |
4 | Astragalin | 11.48 | 54 | 1.97 |
Peaks | Compounds | ABTS (µgTE/mL) | Radical-Scavenging Activity (mAU) | Scavenging Activity Percent (%) |
---|---|---|---|---|
8 | Vanillic acid 4-O-β-d-glucopyranoside | 201.91 | 833 | 35.27 |
9 | Lavandoside | 81.66 | 341 | 9.37 |
10 | 4-hydroxybenzoic acid 4-O-β-d-glucopyranoside | 199.50 | 823 | 20.31 |
12 | Rutin | 143.59 | 594 | 23.20 |
Peaks | Pure Compounds or Extracts | ABTS (µgTE/mL) | Radical-Scavenging Activity (mAU) | ORAC (µMolTE/mg) | TEAC (µMolTE/mg) |
---|---|---|---|---|---|
EtOAc extract | - | - | 18.43 ± 12.58 | 432.06 ± 15.84 | |
n-BuOH extract | - | - | 5.64 ± 0.21 | 430.66 ± 80.25 | |
1 | Protocatechuic acid | 47.59 | 202 | 690.40 ± 45.67 | 469.48 ± 15.71 |
4 | Astragalin | 1.48 | 13 | 300.006 ± 82.71 | 144.69 ± 15.36 |
8 | Vanillic acid 4-O-β-d-glucopyranoside | 16.49 | 75 | 650.91 ± 185.18 | 105.56 ± 8.95 |
9 | Lavandoside | 91.49 | 381 | 37.44 ± 24.74 | 12.14 ± 7.34 |
10 | 4-Hydroxybenzoic acid 4-O-β-d-glucopyranoside | 60.45 | 254 | 434.86 ± 36.43 | 407.94 ± 32.73 |
12 | Rutin | 155.66 | 644 | 612.77 ± 165.45 | 555.66 ± 20.79 |
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Chemam, Y.; Benayache, S.; Marchioni, E.; Zhao, M.; Mosset, P.; Benayache, F. On-Line Screening, Isolation and Identification of Antioxidant Compounds of Helianthemum ruficomum. Molecules 2017, 22, 239. https://doi.org/10.3390/molecules22020239
Chemam Y, Benayache S, Marchioni E, Zhao M, Mosset P, Benayache F. On-Line Screening, Isolation and Identification of Antioxidant Compounds of Helianthemum ruficomum. Molecules. 2017; 22(2):239. https://doi.org/10.3390/molecules22020239
Chicago/Turabian StyleChemam, Yasmine, Samir Benayache, Eric Marchioni, Minjie Zhao, Paul Mosset, and Fadila Benayache. 2017. "On-Line Screening, Isolation and Identification of Antioxidant Compounds of Helianthemum ruficomum" Molecules 22, no. 2: 239. https://doi.org/10.3390/molecules22020239