Structural Analysis and Immuno-Stimulating Activity of an Acidic Polysaccharide from the Stems of Dendrobium nobile Lindl.
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation, Purification and Composition Analysis of DNP-4W
2.2. Structural Characterization of DNP-W4
2.3. Immunological Activity
3. Experimental Section
3.1. Plant Materials and Chemical Reagents
3.2. General Methods
3.3. Isolation and Purification
3.4. Gel-permeation Chromatography (GPC) and Molecular Mass
3.5. Chemical Analysis
3.6. Partial Hydrolysis with Acid
3.7. Periodate Oxidation–Smith Degradation
3.8. Enzymatic Hydrolysis
3.9. Immunobiological Activity Assay
3.10. Statistical Analysis
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Not Available. |
Items | DNP-W4 | DNP-W4E | HDNP-W4 |
---|---|---|---|
Man | 1.0 | 1.0 | 0.1 |
Glc | 4.9 | 5.2 | 4.1 |
Gal | 2.5 | 2.5 | 2.4 |
Xyl | 0.5 | n.d. | 0.1 |
Rha | 1.0 | n.d. | 0.1 |
GalA | 0.9 | n.d. | 0.2 |
Methylated Sugar | Linkages Types | DNP-W4 | HDNP-W4 | DP1 |
---|---|---|---|---|
2,3,4,6-Me4-Glcp | β-d-Glcp-(1→ | 0.5 | Trace | 0.1 |
2,3,6-Me3-Glcp | →4)-β-d-Glcp-(1→ | 1.0 | 1.6 | n.d. |
2,3-Me2-Glcp | →4,6)-β-d-Glcp-(1→ | 1.0 | 0.1 | n.d. |
2,3,4-Me3-Glcp | →6)-β-d-Glcp-(1→ | 1.9 | 2.6 | n.d. |
2,4,6-Me3-Glcp | →3)-β-d-Glcp-(1→ | 0.5 | n.d. | 0.2 |
2,3,4-Me3-Galp | →6)-β-d-Galp-(1→ | 2.0 | 2.4 | n.d. |
2, 4-Me3-Galp | →3,6)-β-d-Galp-(1→ | 0.5 | 0.1 | n.d. |
2,3,4,6-Me3-Manp | β-d-Manp-(1→ | 0.5 | 0.1 | 0.2 |
2,3,6-Me3-Manp | →6)-β-d-Manp-(1→ | 0.6 | Trace | 0.2 |
2,3,4-Me3-Xylp | Xylp-(1→ | 0.5 | 0.1 | 0.4 |
3,4-Me3-Rhap | →2)-α-l-Rhap-(1→ | 1.0 | 0.1 | 1.0 |
2,3,6-Me3-GalAp | →4)-α-d-GalAp-(1→ | 0.9 | 0.2 | 1.0 |
Items | DNP-W4 (OD557) | DNP-W4E (OD557) | HDNP-W4 (OD557) | |||
---|---|---|---|---|---|---|
ConA | LPS | ConA | LPS | ConA | LPS | |
Control | 0.487 ± 0.06 | 0.540 ± 0.04 | 0.487 ± 0.06 | 0.540 ± 0.04 | 0.487 ± 0.06 | 0.540 ± 0.04 |
25 (μg/mL) | 0.468 ± 0.01 | 0.527 ± 0.02 | 0.520 ± 0.05 | 0.553 ± 0.05 | 0.521 ± 0.07 | 0.559 ± 0.04 |
50 (μg/mL) | 0.510 ± 0.05 | 0.577 ± 0.03 | 0.600 ± 0.02 * | 0.600 ± 0.04 | 0.518 ± 0.04 | 0.581 ± 0.02 |
100 (μg/mL) | 0.518 ± 0.08 | 0.573 ± 0.05 | 0.661 ± 0.05 ** | 0.686 ± 0.07 * | 0.614 ± 0.03 * | 0.635 ± 0.05 * |
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Wang, J.-H.; Zuo, S.-R.; Luo, J.-P. Structural Analysis and Immuno-Stimulating Activity of an Acidic Polysaccharide from the Stems of Dendrobium nobile Lindl. Molecules 2017, 22, 611. https://doi.org/10.3390/molecules22040611
Wang J-H, Zuo S-R, Luo J-P. Structural Analysis and Immuno-Stimulating Activity of an Acidic Polysaccharide from the Stems of Dendrobium nobile Lindl. Molecules. 2017; 22(4):611. https://doi.org/10.3390/molecules22040611
Chicago/Turabian StyleWang, Jun-Hui, Shu-Rong Zuo, and Jian-Ping Luo. 2017. "Structural Analysis and Immuno-Stimulating Activity of an Acidic Polysaccharide from the Stems of Dendrobium nobile Lindl." Molecules 22, no. 4: 611. https://doi.org/10.3390/molecules22040611