Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MS
Abstract
:1. Introduction
2. Results and Discussion
2.1. RBC Hematological Changes during Storage
2.2. Metabolomic Analysis of RBCs Stored in MAP under Blood Bank Conditions
2.2.1. Time-Course Changes of Small Molecule Metabolites Involved in Glycolysis during Storage
2.2.2. Time-Course Changes of Small Molecule Metabolites Involved in Pentose Phosphate Pathway during Storage
2.2.3. Time-Course Changes of Small Molecule Metabolites Involved in Glutathione Homeostasis during Storage
2.2.4. Time-Course Changes of Small Molecule Metabolites Involved in Purine Metabolism during Storage
3. Materials and Methods
3.1. Chemicals and Reagents
3.2. Sample Collection and Preparation
3.3. Hematological Analysis
3.4. UPLC-QTOF-MS Conditions
3.5. Data Processing and Statistical Analysis
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Variable | Day 0 | Day 3 | Day 5 | Day 7 | Day 14 | Day 21 | Day 28 | Day 35 | Day 42 | Day 49 |
---|---|---|---|---|---|---|---|---|---|---|
RBC (× 1012/L) | 5.14 ± 0.27 | 5.26 ± 0.31 | 5.31 ± 0.38 | 5.33 ± 0.34 | 5.39 ± 0.34 | 5.32 ± 0.33 | 5.37 ± 0.40 | 5.31 ± 0.29 | 5.32 ± 0.31 | 5.36 ± 0.31 |
HGB (g/dL) | 16.51 ± 0.69 | 16.74 ± 0.74 | 16.89 ± 0.78 | 16.85 ± 0.72 | 16.91 ± 0.69 | 16.81 ± 0.66 | 16.81 ± 0.76 | 16.86 ± 0.72 | 16.83 ± 0.78 | 16.86 ± 0.74 |
HCT (%) | 48.53 ± 2.16 | 49.65 ± 1.77 | 50.35 ± 2.49 | 50.59 ± 2.24 | 50.65 ± 1.80 | 50.35 ± 1.81 | 50.53 ± 2.43 | 50.56 ± 1.99 | 50.64 ± 2.01 | 50.65 ± 1.84 |
MCV (fL) | 94.45 ± 3.36 | 94.50 ± 3.37 | 95.09 ± 3.15 | 95.10 ± 3.25 | 95.13 ± 3.78 | 95.95 ± 3.89 | 96.69 ± 3.43 | 97.33 ± 3.14 | 98.05 ± 3.18 * | 98.73 ± 3.12 * |
MCH (pg) | 32.10 ± 1.12 | 31.80 ± 0.73 | 31.73 ± 1.20 | 31.63 ± 0.86 | 31.45 ± 1.02 | 31.53 ± 1.00 | 31.41 ± 1.22 | 31.44 ± 1.09 | 31.33 ± 0.88 | 31.15 ± 0.76 |
MCHC (g/dL) | 34.00 ± 0.60 | 33.68 ± 0.52 | 33.51 ± 0.67 | 33.39 ± 0.60 | 33.40 ± 0.55 | 33.41 ± 0.58 | 33.40 ± 0.58 | 33.41 ± 0.78 | 33.39 ± 0.87 | 33.36 ± 0.77 |
RDW-CV (%) | 13.28 ± 0.67 | 13.58 ± 0.54 | 13.71 ± 0.55 | 13.75 ± 0.56 | 13.74 ± 0.54 | 13.88 ± 0.58 | 13.89 ± 0.54 | 14.06 ± 0.57 * | 14.35 ± 0.49 ** | 14.70 ± 0.46 *** |
RDW-SD (fL) | 46.55 ± 2.99 | 47.56 ± 2.34 | 48.13 ± 2.81 | 48.21 ± 2.58 | 48.24 ± 2.03 | 48.63 ± 2.58 | 48.83 ± 2.49 | 49.89 ± 2.14 * | 51.24 ± 2.14 ** | 53.21 ± 2.69 *** |
Constituents (mM) | CPDA | MAP |
---|---|---|
NaCl | - | 85 |
NaH2PO4 | 15 | 6 |
Citric acid | 16 | 1 |
Na-citrate | 85 | 5 |
Adenine | 3 | 1.5 |
Dextrose | 160 | 40 |
Mannitol | - | 80 |
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Zhou, Y.; Meng, Z.; Gan, H.; Zheng, Y.; Zhu, X.; Wu, Z.; Li, J.; Gu, R.; Dou, G. Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MS. Molecules 2018, 23, 923. https://doi.org/10.3390/molecules23040923
Zhou Y, Meng Z, Gan H, Zheng Y, Zhu X, Wu Z, Li J, Gu R, Dou G. Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MS. Molecules. 2018; 23(4):923. https://doi.org/10.3390/molecules23040923
Chicago/Turabian StyleZhou, Yong, Zhiyun Meng, Hui Gan, Ying Zheng, Xiaoxia Zhu, Zhuona Wu, Jian Li, Ruolan Gu, and Guifang Dou. 2018. "Time-Course Investigation of Small Molecule Metabolites in MAP-Stored Red Blood Cells Using UPLC-QTOF-MS" Molecules 23, no. 4: 923. https://doi.org/10.3390/molecules23040923