Anti-Helicobacter, Antitubercular and Cytotoxic Activities of Scalaranes from the Red Sea Sponge Hyrtios erectus †
Abstract
:1. Introduction
2. Results and Discussion
2.1. Purification of Compounds 1–15
2.2. Structural Elucidation of Compounds 1–15
2.3. Biological Activities of the Isolated Compounds 1–15
3. Materials and Methods
3.1. General Experimental Procedures
3.2. Biological Materials
3.3. Purification of Compounds 1–15
3.4. Biological Activity of Compounds 1–15
3.4.1. Anti-Helicobacter pylori Activity Assessment
3.4.2. Antitubercular Activity Assessment
3.4.3. Cytotoxic Activity Assessment
3.4.3.1. Cell Culture
3.4.3.2. Trypan-Blue Exclusion Assay
3.4.3.3. Cytotoxic Assessment
3.4.3.4. Statistical Analysis
4. Conclusions
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Not available. |
Position | δC | δH (m, J in Hz) | HMBC (H→C) a |
---|---|---|---|
1 | 39.8, CH2 | 1.73, 0.83 (m) | C-10 |
2 | 18.5, CH2 | 1.63, 1.46 (m) | C-4, C-10 |
3 | 42.0, CH2 | 1.39, 1.15 (m) | C-4 |
4 | 33.2, C | - | - |
5 | 56.4, CH | 0.83 (m) | C-4 |
6 | 18.0, CH2 | 1.58, 1.42 (m) | |
7 | 41.4, CH2 | 1.74, 0.95 (m) | C-8 |
8 | 37.4, C | - | - |
9 | 58.8, CH | 0.93 (m) | C-10, C-12 |
10 | 37.4, C | - | - |
11 | 26.1, CH2 | 1.79, 1.50 (m) | C-10, C-12 |
12 | 80.4, CH | 3.57 (dd, 11.05, 4.25) | C-9, C-11, C-18, C-25 |
12-OH | 4.19 (s) | ||
13 | 39.9, C | - | - |
14 | 52.7, CH | 1.25 (m) | C-8, C-9, C-13, C-16, C-18 |
15 | 23.5, CH2 | 2.17, 2.37 (m) | C-16 |
16 | 136.3, CH | 6.87 (dd, 6.80, 3.40) | C-14, C-15, C-18, C-20 |
17 | 127.3, C | - | - |
18 | 58.7, CH | 2.54 (m) | C-12, C-13, C-25 |
19 | 98.5, CH | 5.74 (d, 5.10) | C-18 |
20 | 166.9, C | - | - |
21 | 21.3, CH3 | 0.81 (s) | C-4 |
22 | 33.2, CH3 | 0.83 (s) | C-4 |
23 | 16.5, CH3 | 0.85 (s) | C-1, C-5, C-9, C-10 |
24 | 16.7, CH3 | 0.93 (s) | C-7, C-8, C-9, C-14 |
25 | 9.1, CH3 | 0.86 (s) | C-12, C-13, C-14, C-18 |
Compound | Anti-H. pylori (MIC) | Anti-TB (MIC) | Cytotoxic (IC50 ± SEM) | ||
---|---|---|---|---|---|
MCF-7 | HCT-116 | HepG2 | |||
1 | 4.39 | 0.54 | 24.6 ± 2.3 | 25.5 ± 3.3 | 19.8 ± 1.4 |
2 | NA | 16.00 | 1.2 ± 0.1 | 0.4 ± 0.1 | 1.1 ± 0.1 |
3 | 10.10 | 5.05 | NA | NA | NA |
4 | 9.11 | 1.12 | 25.9 ± 1.9 | 17.5 ± 1.3 | 24.7 ± 4.8 |
5 | 146.02 | 9.13 | 22.0 ± 0.4 | 15.2 ± 2.0 | 15.3 ± 1.1 |
6 | 8.78 | 4.39 | - | - | - |
7 | 20.23 | 5.05 | 1.6 ± 0.1 | 1.4 ± 0.05 | 1.6 ± 0.5 |
8 | 80.95 | 10.12 | 32.7 ± 3.2 | 34.5 ± 7.5 | 23.5 ± 1.8 |
9 | 77.73 | 19.42 | NA | NA | NA |
10 | 8.47 | 4.23 | 12.4 ± 0.9 | 2.9 ± 0.7 | 5.5 ± 0.9 |
11 | 263.71 | 16.47 | 55.6 ± 2.1 | 17.8 ± 1.9 | 20.1 ± 1.5 |
12 | 32.97 | 8.24 | 37.3 ± 5.5 | 22.8 ± 2.2 | 34.9 ± 6.1 |
13 | 16.03 | 8.02 | 54.2 ± 3.3 | 26.5 ± 1.1 | 26.6 ± 3.8 |
14 | 81.38 | 20.33 | NA | NA | NA |
15 | 33.97 | 16.97 | 34.9 ± 4.9 | 48.6 ± 7.2 | 27.3 ± 3.3 |
isoniazid | ---- | 0.87 | ---- | ---- | ---- |
clarithromycin | 1.31 | ---- | ---- | ---- | ---- |
doxorubicin | ---- | ---- | 0.41 ± 0.1 | 0.11 ± 0.04 | 0.85 ± 0.1 |
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Share and Cite
Alahdal, A.M.; Asfour, H.Z.; Ahmed, S.A.; Noor, A.O.; Al-Abd, A.M.; Elfaky, M.A.; Elhady, S.S. Anti-Helicobacter, Antitubercular and Cytotoxic Activities of Scalaranes from the Red Sea Sponge Hyrtios erectus. Molecules 2018, 23, 978. https://doi.org/10.3390/molecules23040978
Alahdal AM, Asfour HZ, Ahmed SA, Noor AO, Al-Abd AM, Elfaky MA, Elhady SS. Anti-Helicobacter, Antitubercular and Cytotoxic Activities of Scalaranes from the Red Sea Sponge Hyrtios erectus. Molecules. 2018; 23(4):978. https://doi.org/10.3390/molecules23040978
Chicago/Turabian StyleAlahdal, Abdulrahman M., Hani Z. Asfour, Safwat A. Ahmed, Ahmad O. Noor, Ahmed M. Al-Abd, Mahmoud A. Elfaky, and Sameh S. Elhady. 2018. "Anti-Helicobacter, Antitubercular and Cytotoxic Activities of Scalaranes from the Red Sea Sponge Hyrtios erectus" Molecules 23, no. 4: 978. https://doi.org/10.3390/molecules23040978