Preparation, Characterization and Biological Applications of Biosynthesized Silver Nanoparticles with Chitosan-Fucoidan Coating
Abstract
:1. Introduction
2. Results and Discussion
2.1. Preparation of Chitosan–Fucoidan Complex-Coated AgNPs
2.2. UV-Visible (UV-Vis) Spectroscopy
2.3. Particle Size Analysis Using DLS
2.4. Fourier Transform Infrared (FT-IR) Spectroscopy
2.5. Morphology of AgNPs
2.6. Antibacterial Activity of AgNPs
2.7. Anticancer Activity of Chitosan–Fucoidan Complex-Coated AgNPs
2.8. Flow Cytometry Analysis
3. Materials and Methods
3.1. Preparation of Fucoidan-Coated AgNPs
3.2. UV-Visible Spectroscopy
3.3. DLS Analysis
3.4. Fourier Transform Infrared Spectroscopy
3.5. Transmission Electron Microscopy Analysis
3.6. Antimicrobial Activity Test of AgNPs
3.7. Cytotoxicity Assay
3.8. Cytotoxicity Effects of Nanoparticles in Cancer Cells
3.9. Optical Microscopy Analysis
3.10. Apoptosis Analysis
3.11. Statistical Analysis
4. Conclusions
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
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Venkatesan, J.; Singh, S.K.; Anil, S.; Kim, S.-K.; Shim, M.S. Preparation, Characterization and Biological Applications of Biosynthesized Silver Nanoparticles with Chitosan-Fucoidan Coating. Molecules 2018, 23, 1429. https://doi.org/10.3390/molecules23061429
Venkatesan J, Singh SK, Anil S, Kim S-K, Shim MS. Preparation, Characterization and Biological Applications of Biosynthesized Silver Nanoparticles with Chitosan-Fucoidan Coating. Molecules. 2018; 23(6):1429. https://doi.org/10.3390/molecules23061429
Chicago/Turabian StyleVenkatesan, Jayachandran, Sandeep Kumar Singh, Sukumaran Anil, Se-Kwon Kim, and Min Suk Shim. 2018. "Preparation, Characterization and Biological Applications of Biosynthesized Silver Nanoparticles with Chitosan-Fucoidan Coating" Molecules 23, no. 6: 1429. https://doi.org/10.3390/molecules23061429