Pomolic Acid Ameliorates Fibroblast Activation and Renal Interstitial Fibrosis through Inhibition of SMAD-STAT Signaling Pathways
Abstract
:1. Introduction
2. Results
2.1. PA Improves Histopathological Changes in UUO Mice
2.2. PA Attenuates UUO-Induced EMT Progression
2.3. PA Inhibits TGF-β1 Stimulated Fibroblast Activation
2.4. PA Inhibits TGF-β1 Induced Canonical and Non-Canonical Signaling
3. Discussion
4. Materials and Methods
4.1. Cell Cultures and Reagents
4.2. Cytotoxicity Assay
4.3. Induction of UUO Injury
4.4. Pathology
4.5. Immunoblot Analysis
4.6. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
TGF-β1 | Transforming growth factor β1 |
SMAD3 | Mothers against decapentaplegic homolog 3 |
STAT3 | Signal transducer and activator of transcription 3 |
PA | Pomolic acid |
ECM | Extracellular matrix |
EMT | Epithelial-mesenchymal transition |
α-SMA | α-Smooth muscle actin |
mTOR | Mammalian target of rapamycin |
DMSO | Dimethylsulfoxide |
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Sample Availability: Samples of the compounds are available from the authors. |
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Park, J.-H.; Jang, K.M.; An, H.J.; Kim, J.-Y.; Gwon, M.-G.; Gu, H.; Park, B.; Park, K.-K. Pomolic Acid Ameliorates Fibroblast Activation and Renal Interstitial Fibrosis through Inhibition of SMAD-STAT Signaling Pathways. Molecules 2018, 23, 2236. https://doi.org/10.3390/molecules23092236
Park J-H, Jang KM, An HJ, Kim J-Y, Gwon M-G, Gu H, Park B, Park K-K. Pomolic Acid Ameliorates Fibroblast Activation and Renal Interstitial Fibrosis through Inhibition of SMAD-STAT Signaling Pathways. Molecules. 2018; 23(9):2236. https://doi.org/10.3390/molecules23092236
Chicago/Turabian StylePark, Ji-Hyun, Kyung Mi Jang, Hyun Jin An, Jung-Yeon Kim, Mi-Gyeong Gwon, Hyemin Gu, Byoungduck Park, and Kwan-Kyu Park. 2018. "Pomolic Acid Ameliorates Fibroblast Activation and Renal Interstitial Fibrosis through Inhibition of SMAD-STAT Signaling Pathways" Molecules 23, no. 9: 2236. https://doi.org/10.3390/molecules23092236