Heat-Killed Fusobacterium nucleatum Triggers Varying Heme-Related Inflammatory and Stress Responses Depending on Primary Human Respiratory Epithelial Cell Type
Abstract
:1. Introduction
2. Results
2.1. Secreted IL-8 and IL-6 Are Affected by Bacterial Incubation Time and Cell Count
2.2. Heat-Killed Fn Affected the Extracellular Secretion of Inflammatory- and Stress-Related Signals
2.3. Centrality Analysis of Biomolecules Affected by Heat-Killed Fn
3. Discussion
4. Materials and Methods
4.1. Bacterial Cell Culture and Heat Inactivation
4.2. Primary Human Epithelial Cell Culture and Treatment
4.3. Measurement of Secreted Inflammation- and Stress-Related Signals
4.4. Network Design and Analysis
4.5. Statistical Analyses
4.6. Statement on Reproducibility
Author Contributions
Funding
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
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Koike, R.; Cueno, M.E.; Nodomi, K.; Tamura, M.; Kamio, N.; Tanaka, H.; Kotani, A.; Imai, K. Heat-Killed Fusobacterium nucleatum Triggers Varying Heme-Related Inflammatory and Stress Responses Depending on Primary Human Respiratory Epithelial Cell Type. Molecules 2020, 25, 3839. https://doi.org/10.3390/molecules25173839
Koike R, Cueno ME, Nodomi K, Tamura M, Kamio N, Tanaka H, Kotani A, Imai K. Heat-Killed Fusobacterium nucleatum Triggers Varying Heme-Related Inflammatory and Stress Responses Depending on Primary Human Respiratory Epithelial Cell Type. Molecules. 2020; 25(17):3839. https://doi.org/10.3390/molecules25173839
Chicago/Turabian StyleKoike, Ryo, Marni E. Cueno, Keiko Nodomi, Muneaki Tamura, Noriaki Kamio, Hajime Tanaka, Ai Kotani, and Kenichi Imai. 2020. "Heat-Killed Fusobacterium nucleatum Triggers Varying Heme-Related Inflammatory and Stress Responses Depending on Primary Human Respiratory Epithelial Cell Type" Molecules 25, no. 17: 3839. https://doi.org/10.3390/molecules25173839