Applications of Gelatin Methacryloyl (GelMA) Hydrogels in Microfluidic Technique-Assisted Tissue Engineering
Abstract
:1. Introduction
2. Applications of GelMA Hydrogels as Raw Materials for Tissue Engineering Building Blocks
2.1. Raw Materials for Microfibers
2.2. Bioink for Complex Structures in Microfluidic Bioprinting Platforms
3. Applications of GelMA Hydrogels as Simulation Units in Tissue Engineering
3.1. Scaffolds for 3-D Cell Culture
3.2. Components for Organs-On-A-Chip
4. Conclusions and Outlook
Author Contributions
Funding
Conflicts of Interest
References
- Sharma, P.; Kumar, P.; Sharma, R.; Bhatt, V.D.; Dhot, P.S. Tissue Engineering; Current Status & Futuristic Scope. J. Med. Life 2019, 12, 225–229. [Google Scholar] [CrossRef] [PubMed]
- Kang, H.W.; Lee, S.J.; Ko, I.K.; Kengla, C.; Yoo, J.J.; Atala, A. A 3D bioprinting system to produce human-scale tissue constructs with structural integrity. Nat. Biotechnol. 2016, 34, 312–319. [Google Scholar] [CrossRef] [PubMed]
- Langer, R.; Vacanti, J.P. Tissue engineering. Science 1993, 260, 920–926. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Jun, Y.; Kang, E.; Chae, S.; Lee, S.H. Microfluidic spinning of micro- and nano-scale fibers for tissue engineering. Lab Chip 2014, 14, 2145–2160. [Google Scholar] [CrossRef] [PubMed]
- Chung, B.G.; Lee, K.H.; Khademhosseini, A.; Lee, S.H. Microfluidic fabrication of microengineered hydrogels and their application in tissue engineering. Lab Chip 2012, 12, 45–59. [Google Scholar] [CrossRef] [PubMed]
- Ikada, Y. Challenges in tissue engineering. J. R. Soc. Interface 2006, 3, 589–601. [Google Scholar] [CrossRef] [PubMed]
- Khademhosseini, A.; Langer, R. Microengineered hydrogels for tissue engineering. Biomaterials 2007, 28, 5087–5092. [Google Scholar] [CrossRef] [PubMed]
- Khademhosseini, A.; Langer, R.; Borenstein, J.; Vacanti, J.P. Microscale technologies for tissue engineering and biology. Proc. Natl. Acad. Sci. USA 2006, 103, 2480–2487. [Google Scholar] [CrossRef] [Green Version]
- Dinh, N.D.; Luo, R.; Christine, M.T.A.; Lin, W.N.; Shih, W.C.; Goh, J.C.; Chen, C.H. Effective Light Directed Assembly of Building Blocks with Microscale Control. Small 2017, 13, 1700684. [Google Scholar] [CrossRef]
- Xu, F.; Moon, S.J.; Emre, A.E.; Turali, E.S.; Song, Y.S.; Hacking, S.A.; Nagatomi, J.; Demirci, U. A droplet-based building block approach for bladder smooth muscle cell (SMC) proliferation. Biofabrication 2010, 2, 014105. [Google Scholar] [CrossRef]
- Tien, J.; Dance, Y.W. Microfluidic Biomaterials. Adv. Healthc. Mater. 2020, e2001028. [Google Scholar] [CrossRef] [PubMed]
- Sahiner, N.; Sagbas, S. Multifunctional tunable p(inulin) microgels. Mater. Sci. Eng. C Mater. Biol. Appl. 2014, 40, 366–372. [Google Scholar] [CrossRef] [PubMed]
- Tekin, H.; Tsinman, T.; Sanchez, J.G.; Jones, B.J.; Camci-Unal, G.; Nichol, J.W.; Langer, R.; Khademhosseini, A. Responsive Micromolds for Sequential Patterning of Hydrogel Microstructures. J. Am. Chem. Soc. 2011, 133, 12944–12947. [Google Scholar] [CrossRef] [Green Version]
- Selimović, S.; Oh, J.; Bae, H.; Dokmeci, M.; Khademhosseini, A. Microscale Strategies for Generating Cell-Encapsulating Hydrogels. Polymers 2012, 4, 1554–1579. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Yanagawa, F.; Sugiura, S.; Kanamori, T. Hydrogel microfabrication technology toward three dimensional tissue engineering. Regen. Ther. 2016, 3, 45–57. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Mark, D.; Haeberle, S.; Roth, G.; Von Stetten, F.; Zengerle, R. Microfluidic lab-on-a-chip platforms: Requirements, characteristics and applications. Chem. Soc. Rev. 2010, 39, 1153–1182. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Colosi, C.; Costantini, M.; Barbetta, A.; Dentini, M. Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs. Methods Mol. Biol. 2017, 1612, 369–380. [Google Scholar] [CrossRef]
- Richard, C.; Neild, A.; Cadarso, V.J. The emerging role of microfluidics in multi-material 3D bioprinting. Lab Chip 2020, 20, 2044–2056. [Google Scholar] [CrossRef]
- Costantini, M.; Colosi, C.; Święszkowski, W.; Barbetta, A. Co-axial wet-spinning in 3D bioprinting: State of the art and future perspective of microfluidic integration. Biofabrication 2018, 11, 012001. [Google Scholar] [CrossRef]
- Meyvantsson, I.; Beebe, D.J. Cell Culture Models in Microfluidic Systems. Annu. Rev. Anal. Chem. 2008, 1, 423–449. [Google Scholar] [CrossRef] [Green Version]
- Lu, T.; Li, Y.; Chen, T. Techniques for fabrication and construction of three-dimensional scaffolds for tissue engineering. Int. J. Nanomed. 2013, 8, 337–350. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Bertassoni, L.E.; Cardoso, J.; Manoharan, V.; Cristino, A.L.; Bhise, N.S.; Araujo, W.A.; Zorlutuna, P.; Vrana, N.E.; Ghaemmaghami, A.M.; Dokmeci, M.R.; et al. Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels. Biofabrication 2014, 6, 024105. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Li, N.; Zhang, W.; Li, Y.; Lin, J.M. Analysis of cellular biomolecules and behaviors using microfluidic chip and fluorescence method. TrAC Trends Anal. Chem. 2019, 117, 200–214. [Google Scholar] [CrossRef]
- Pennacchio, F.A.; Casale, C.; Urciuolo, F.; Imparato, G.; Vecchione, R.; Netti, P.A. Controlling the orientation of a cell-synthesized extracellular matrix by using engineered gelatin-based building blocks. Biomater. Sci. 2018, 6, 2084–2091. [Google Scholar] [CrossRef]
- Van Den Bulcke, A.I.; Bogdanov, B.; De Rooze, N.; Schacht, E.H.; Cornelissen, M.; Berghmans, H. Structural and Rheological Properties of Methacrylamide Modified Gelatin Hydrogels. Biomacromolecules 2000, 1, 31–38. [Google Scholar] [CrossRef] [PubMed]
- Amonpattaratkit, P.; Khunmanee, S.; Kim, D.H.; Park, H. Synthesis and Characterization of Gelatin-Based Crosslinkers for the Fabrication of Superabsorbent Hydrogels. Materials 2017, 10, 826. [Google Scholar] [CrossRef] [Green Version]
- Lin, R.Z.; Chen, Y.C.; Moreno-Luna, R.; Khademhosseini, A.; Melero-Martin, J.M. Transdermal regulation of vascular network bioengineering using a photopolymerizable methacrylated gelatin hydrogel. Biomaterials 2013, 34, 6785–6796. [Google Scholar] [CrossRef] [Green Version]
- Billiet, T.; Gevaert, E.; De Schryver, T.; Cornelissen, M.; Dubruel, P. The 3D printing of gelatin methacrylamide cell-laden tissue-engineered constructs with high cell viability. Biomaterials 2014, 35, 49–62. [Google Scholar] [CrossRef]
- Chen, M.B.; Srigunapalan, S.; Wheeler, A.R.; Simmons, C.A. A 3D microfluidic platform incorporating methacrylated gelatin hydrogels to study physiological cardiovascular cell-cell interactions. Lab Chip 2013, 13, 2591–2598. [Google Scholar] [CrossRef]
- Loessner, D.; Meinert, C.; Kaemmerer, E.; Martine, L.C.; Yue, K.; Levett, P.A.; Klein, T.J.; Melchels, F.P.; Khademhosseini, A.; Hutmacher, D.W. Functionalization, preparation and use of cell-laden gelatin methacryloyl-based hydrogels as modular tissue culture platforms. Nat. Protoc. 2016, 11, 727–746. [Google Scholar] [CrossRef] [Green Version]
- Klotz, B.J.; Gawlitta, D.; Rosenberg, A.J.W.P.; Malda, J.; Melchels, F.P.W. Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair. Trends Biotechnol. 2016, 34, 394–407. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Liu, W.; Heinrich, M.A.; Zhou, Y.; Akpek, A.; Hu, N.; Liu, X.; Guan, X.; Zhong, Z.; Jin, X.; Khademhosseini, A.; et al. Extrusion Bioprinting of Shear-Thinning Gelatin Methacryloyl Bioinks. Adv. Healthc. Mater. 2017, 6, 201601451. [Google Scholar] [CrossRef] [PubMed]
- Erdem, A.; Darabi, M.A.; Nasiri, R.; Sangabathuni, S.; Ertas, Y.N.; Alem, H.; Hosseini, V.; Shamloo, A.; Nasr, A.S.; Ahadian, S.; et al. 3D Bioprinting of Oxygenated Cell-Laden Gelatin Methacryloyl Constructs. Adv. Healthc. Mater. 2020, 9, e1901794. [Google Scholar] [CrossRef] [PubMed]
- Wu, J.; Li, G.; Ye, T.; Lu, G.; Li, R.; Deng, L.; Wang, L.; Cai, M.; Cui, W. Stem cell-laden injectable hydrogel microspheres for cancellous bone regeneration. Chem. Eng. J. 2020, 393, 124715. [Google Scholar] [CrossRef]
- Fedorovich, N.E.; Oudshoorn, M.H.; Van Geemen, D.; Hennink, W.E.; Alblas, J.; Dhert, W.J. The effect of photopolymerization on stem cells embedded in hydrogels. Biomaterials 2009, 30, 344–353. [Google Scholar] [CrossRef] [PubMed]
- Sun, M.; Sun, X.; Wang, Z.; Guo, S.; Yu, G.; Yang, H. Synthesis and Properties of Gelatin Methacryloyl (GelMA) Hydrogels and Their Recent Applications in Load-Bearing Tissue. Polymers 2018, 10, 1290. [Google Scholar] [CrossRef] [Green Version]
- Parkatzidis, K.; Kabouraki, E.; Selimis, A.; Kaliva, M.; Ranella, A.; Farsari, M. Initiator-Free, Multiphoton Polymerization of Gelatin Methacrylamide. Macromol. Mater. Eng. 2018, 303, 1800458. [Google Scholar] [CrossRef]
- Zhang, Q.; Qian, C.; Xiao, X.; Zhu, C.; Cui, W. Development of a visible light, cross-linked GelMA hydrogel containing decellularized human amniotic particles as a soft tissue replacement for oral mucosa repair. RSC Adv. 2019, 9, 18344–18352. [Google Scholar] [CrossRef] [Green Version]
- Wang, Z.; Tian, Z.; Jin, X.; Holzman, J.F.; Menard, F.; Keekyoung, K. Visible light-based stereolithography bioprinting of cell-adhesive gelatin hydrogels. In Proceedings of the 39th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, Jeju Island, Korea, 11–15 July 2017; pp. 1599–1602. [Google Scholar]
- Kerscher, P.; Kaczmarek, J.A.; Head, S.E.; Ellis, M.E.; Seeto, W.J.; Kim, J.; Bhattacharya, S.; Suppiramaniam, V.; Lipke, E.A. Direct Production of Human Cardiac Tissues by Pluripotent Stem Cell Encapsulation in Gelatin Methacryloyl. ACS Biomater. Sci. Eng. 2017, 3, 1499–1509. [Google Scholar] [CrossRef]
- Adib, A.A.; Sheikhi, A.; Shahhosseini, M.; Simeunović, A.; Wu, S.; Castro, C.E.; Zhao, R.; Khademhosseini, A.; Hoelzle, D.J. Direct-write 3D printing and characterization of a GelMA-based biomaterial for intracorporeal tissue. Biofabrication 2020, 12, 045006. [Google Scholar] [CrossRef]
- Lim, K.S.; Abinzano, F.; Bernal, P.N.; Sanchez, A.A.; Atienza-Roca, P.; Otto, I.A.; Peiffer, Q.C.; Matsusaki, M.; Woodfield, T.; Malda, J.; et al. One-Step Photoactivation of a Dual-Functionalized Bioink as Cell Carrier and Cartilage-Binding Glue for Chondral Regeneration. Adv. Healthc. Mater. 2020, 9, e1901792. [Google Scholar] [CrossRef] [PubMed]
- Uehara, M.; Li, X.; Sheikhi, A.; Zandi, N.; Walker, B.; Saleh, B.; Banouni, N.; Jiang, L.; Ordikhani, F.; Dai, L.; et al. Anti-IL-6 eluting immunomodulatory biomaterials prolong skin allograft survival. Sci. Rep. 2019, 9, 6535. [Google Scholar] [CrossRef] [PubMed]
- Suo, H.; Zhang, D.; Yin, J.; Qian, J.; Wu, Z.L.; Fu, J. Interpenetrating polymer network hydrogels composed of chitosan and photocrosslinkable gelatin with enhanced mechanical properties for tissue engineering. Mater. Sci. Eng. C Mater. Biol. Appl. 2018, 92, 612–620. [Google Scholar] [CrossRef] [PubMed]
- Wang, Z.; Tian, Z.; Menard, F.; Kim, K. Comparative study of gelatin methacrylate hydrogels from different sources for biofabrication applications. Biofabrication 2017, 9, 044101. [Google Scholar] [CrossRef] [PubMed]
- Zhao, X.; Lang, Q.; Yildirimer, L.; Lin, Z.Y.; Cui, W.; Annabi, N.; Ng, K.W.; Dokmeci, M.R.; Ghaemmaghami, A.M.; Khademhosseini, A. Photocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering. Adv. Healthc. Mater. 2016, 5, 108–118. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Adrus, N.; Ulbricht, M. Rheological studies on PNIPAAm hydrogel synthesis via in situ polymerization and on resulting viscoelastic properties. React. Funct. Polym. 2013, 73, 141–148. [Google Scholar] [CrossRef]
- Cao, D.; Zhang, Y.; Cui, Z.; Du, Y.; Shi, Z. New strategy for design and fabrication of polymer hydrogel with tunable porosity as artificial corneal skirt. Mater. Sci. Eng. C Mater. Biol. Appl. 2017, 70 Pt 1, 665–672. [Google Scholar] [CrossRef]
- Schuurman, W.; Levett, P.A.; Pot, M.W.; Van Weeren, P.R.; Dhert, W.J.; Hutmacher, D.W.; Melchels, F.P.; Klein, T.J.; Malda, J. Gelatin-Methacrylamide Hydrogels as Potential Biomaterials for Fabrication of Tissue-Engineered Cartilage Constructs. Macromol. Biosci. 2013, 13, 551–561. [Google Scholar] [CrossRef]
- Zhao, X.; Sun, X.; Yildirimer, L.; Lang, Q.; Lin, Z.Y.; Zheng, R.; Zhang, Y.; Cui, W.; Annabi, N.; Khademhosseini, A. Cell infiltrative hydrogel fibrous scaffolds for accelerated wound healing. Acta Biomater. 2017, 49, 66–77. [Google Scholar] [CrossRef] [Green Version]
- Lai, T.C.; Yu, J.; Tsai, W.B. Gelatin methacrylate/carboxybetaine methacrylate hydrogels with tunable crosslinking for controlled drug release. J. Mater. Chem. B 2016, 4, 2304–2313. [Google Scholar] [CrossRef]
- Rizwan, M.; Chan, S.W.; Comeau, P.A.; Willett, T.L.; Yim, E. Effect of sterilization treatment on mechanical properties, biodegradation, bioactivity and printability of GelMA hydrogels. Biomed. Mater. 2020. [Google Scholar] [CrossRef] [PubMed]
- Wu, Y.; Xiang, Y.; Fang, J.; Li, X.; Lin, Z.; Dai, G.; Yin, J.; Wei, P.; Zhang, D. The influence of the stiffness of GelMA substrate on the outgrowth of PC12 cells. Biosci. Rep. 2019, 39, 20181748. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Nichol, J.W.; Koshy, S.T.; Bae, H.; Hwang, C.M.; Yamanlar, S.; Khademhosseini, A. Cell-laden microengineered gelatin methacrylate hydrogels. Biomaterials 2010, 31, 5536–5544. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Kimlinger, D.F.; Thomas, D.G.; Vander Wiel, J.B.; Chen, Y.; Montazami, R.; Hashemi, N.N. Microfibers as Physiologically Relevant Platforms for Creation of 3D Cell Cultures. Macromol. Biosci. 2017, 17, 1700279. [Google Scholar] [CrossRef] [Green Version]
- Onoe, H.; Takeuchi, S. Cell-laden microfibers for bottom-up tissue engineering. Drug Discov. Today 2015, 20, 236–246. [Google Scholar] [CrossRef]
- Liu, W.; Zhong, Z.; Hu, N.; Zhou, Y.; Maggio, L.; Miri, A.K.; Fragasso, A.; Jin, X.; Khademhosseini, A.; Zhang, Y.S. Coaxial extrusion bioprinting of 3D microfibrous constructs with cell-favorable gelatin methacryloyl microenvironments. Biofabrication 2018, 10, 024102. [Google Scholar] [CrossRef]
- Zhang, Y.S.; Pi, Q.; Van Genderen, A.M. Microfluidic Bioprinting for Engineering Vascularized Tissues and Organoids. J. Vis. Exp. 2017, 126, e55957. [Google Scholar] [CrossRef]
- Liu, X.; Zuo, Y.; Sun, J.; Guo, Z.; Fan, H.; Zhang, X. Degradation regulated bioactive hydrogel as the bioink with desirable moldability for microfluidic biofabrication. Carbohydr. Polym. 2017, 178, 8–17. [Google Scholar] [CrossRef]
- Shi, X.; Ostrovidov, S.; Zhao, Y.; Liang, X.; Kasuya, M.; Kurihara, K.; Nakajima, K.; Bae, H.; Yan, H.; Khademhosseini, A. Microfluidic Spinning of Cell-Responsive Grooved Microfibers. Adv. Funct. Mater. 2015, 25, 2250–2259. [Google Scholar] [CrossRef]
- Shao, L.; Gao, Q.; Zhao, H.; Xie, C.; Fu, J.; Liu, Z.; Xiang, M.; He, Y. Fiber-Based Mini Tissue with Morphology-Controllable GelMA Microfibers. Small 2018, 14, e1802187. [Google Scholar] [CrossRef]
- Shao, L.; Gao, Q.; Xie, C.; Fu, J.; Xiang, M.; He, Y. Bioprinting of Cell-Laden Microfiber: Can It Become a Standard Product? Adv. Healthc. Mater. 2019, 8, e1900014. [Google Scholar] [CrossRef] [PubMed]
- Zuo, Y.; He, X.; Yang, Y.; Wei, D.; Sun, J.; Zhong, M.; Xie, R.; Fan, H.; Zhang, X. Microfluidic-based generation of functional microfibers for biomimetic complex tissue construction. Acta Biomater. 2016, 38, 153–162. [Google Scholar] [CrossRef] [PubMed]
- Cheng, Y.; Yu, Y.; Fu, F.; Wang, J.; Shang, L.; Gu, Z.; Zhao, Y. Controlled Fabrication of Bioactive Microfibers for Creating Tissue Constructs Using Microfluidic Techniques. ACS Appl. Mater. Interfaces 2016, 8, 1080–1086. [Google Scholar] [CrossRef] [PubMed]
- Chen, C.; Tang, J.; Gu, Y.; Liu, L.; Liu, X.; Deng, L.; Martins, C.; Sarmento, B.; Cui, W.; Chen, L. Tissue Regeneration: Bioinspired Hydrogel Electrospun Fibers for Spinal Cord Regeneration. Adv. Funct. Mater. 2019, 29, 1806899. [Google Scholar] [CrossRef]
- Ebrahimi, M.; Ostrovidov, S.; Salehi, S.; Kim, S.B.; Bae, H.; Khademhosseini, A. Enhanced skeletal muscle formation on microfluidic spun gelatin methacryloyl (GelMA) fibres using surface patterning and agrin treatment. J. Tissue Eng. Regen. Med. 2018, 12, 2151–2163. [Google Scholar] [CrossRef]
- Sun, T.; Yao, Y.; Shi, Q.; Wang, H.; Dario, P.; Sun, J.; Huang, Q.; Fukuda, T. Template-based fabrication of spatially organized 3D bioactive constructs using magnetic low-concentration gelation methacrylate (GelMA) microfibers. Soft Matter 2020, 16, 3902–3913. [Google Scholar] [CrossRef]
- Mandrycky, C.; Wang, Z.; Kim, K.; Kim, D.H. 3D bioprinting for engineering complex tissues. Biotechnol. Adv. 2016, 34, 422–434. [Google Scholar] [CrossRef] [Green Version]
- Miri, A.K.; Nieto, D.; Iglesias, L.; Hosseinabadi, H.G.; Maharjan, S.; Ruiz-Esparza, G.U.; Khoshakhlagh, P.; Manbachi, A.; Dokmeci, M.R.; Chen, S.; et al. Microfluidics-Enabled Multimaterial Maskless Stereolithographic Bioprinting. Adv. Mater. 2018, 30, e1800242. [Google Scholar] [CrossRef]
- Andersson, H.; van den Berg, A. Microfabrication and microfluidics for tissue engineering: State of the art and future opportunities. Lab Chip 2004, 4, 98–103. [Google Scholar] [CrossRef]
- Colosi, C.; Shin, S.R.; Manoharan, V.; Massa, S.; Costantini, M.; Barbetta, A.; Dokmeci, M.R.; Dentini, M.; Khademhosseini, A. Microfluidic Bioprinting of Heterogeneous 3D Tissue Constructs Using Low-Viscosity Bioink. Adv. Mater. 2016, 28, 677–684. [Google Scholar] [CrossRef]
- Chen, J.; Huang, D.; Wang, L.; Hou, J.; Zhang, H.; Li, Y.; Zhong, S.; Wang, Y.; Wu, Y.; Huang, W. 3D bioprinted multiscale composite scaffolds based on gelatin methacryloyl (GelMA)/chitosan microspheres as a modular bioink for enhancing 3D neurite outgrowth and elongation. J. Colloid Interface Sci. 2020, 574, 162–173. [Google Scholar] [CrossRef] [PubMed]
- Lee, J.; Kim, G. A cryopreservable cell-laden GelMa-based scaffold fabricated using a 3D printing process supplemented with an in situ photo-crosslinking. J. Ind. Eng. Chem. 2020, 85, 249–257. [Google Scholar] [CrossRef]
- Knowlton, S.; Yu, C.H.; Ersoy, F.; Emadi, S.; Khademhosseini, A.; Tasoglu, S. 3D-printed microfluidic chips with patterned, cell-laden hydrogel constructs. Biofabrication 2016, 8, 025019. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Mansoorifar, A.; Tahayeri, A.; Bertassoni, L.E. Bioinspired reconfiguration of 3D printed microfluidic hydrogels via automated manipulation of magnetic inks. Lab Chip 2020, 20, 1713–1719. [Google Scholar] [CrossRef]
- Lee, J.M.; Seo, H.I.; Bae, J.H.; Chung, B.G. Hydrogel microfluidic co-culture device for photothermal therapy and cancer migration. Electrophoresis 2017, 38, 1318–1324. [Google Scholar] [CrossRef]
- Li, R.; Lv, X.; Zhang, X.; Saeed, O.; Deng, Y. Microfluidics for cell-cell interactions: A review. Front. Chem. Sci. Eng. 2016, 10, 90–98. [Google Scholar] [CrossRef]
- Sung, J.H.; Kam, C.; Shuler, M.L. A microfluidic device for a pharmacokinetic-pharmacodynamic (PK-PD) model on a chip. Lab Chip 2010, 10, 446–455. [Google Scholar] [CrossRef]
- Pepelanova, I.; Kruppa, K.; Scheper, T.; Lavrentieva, A. Gelatin-Methacryloyl (GelMA) Hydrogels with Defined Degree of Functionalization as a Versatile Toolkit for 3D Cell Culture and Extrusion Bioprinting. Bioengineering 2018, 5, 55. [Google Scholar] [CrossRef] [Green Version]
- Nestor, B.A.; Samiei, E.; Samanipour, R.; Gupta, A.; Van den Berg, A.; Diaz de Leon Derby, M.; Wang, Z.; Nejad, H.R.; Kim, K.; Hoorfar, M. Digital microfluidic platform for dielectrophoretic patterning of cells encapsulated in hydrogel droplets. RSC Adv. 2016, 6, 57409–57416. [Google Scholar] [CrossRef]
- Zhang, X.; Li, J.; Ye, P.; Gao, G.; Hubbell, K.; Cui, X. Coculture of mesenchymal stem cells and endothelial cells enhances host tissue integration and epidermis maturation through AKT activation in gelatin methacryloyl hydrogel-based skin model. Acta Biomater. 2017, 59, 317–326. [Google Scholar] [CrossRef]
- Antunes, J.; Gaspar, V.M.; Ferreira, L.; Monteiro, M.; Henrique, R.; Jerónimo, C.; Mano, J.F. In-air production of 3D co-culture tumor spheroid hydrogels for expedited drug screening. Acta Biomater. 2019, 94, 392–409. [Google Scholar] [CrossRef] [PubMed]
- Takeuchi, M.; Kozuka, T.; Kim, E.; Ichikawa, A.; Hasegawa, Y.; Huang, Q.; Fukuda, T. On-Chip Fabrication of Cell-Attached Microstructures using Photo-Cross-Linkable Biodegradable Hydrogel. J. Funct. Biomater. 2020, 11, 18. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Cha, C.; Oh, J.; Kim, K.; Qiu, Y.; Joh, M.; Shin, S.R.; Wang, X.; Camci-Unal, G.; Wan, K.-T.; Liao, R.; et al. Microfluidics-Assisted Fabrication of Gelatin-Silica Core–Shell Microgels for Injectable Tissue Constructs. Biomacromolecules 2014, 15, 283–290. [Google Scholar] [CrossRef] [PubMed]
- Rahimi, R.; Ochoa, M.; Donaldson, A.; Parupudi, T.; Dokmeci, M.R.; Khademhosseini, A.; Ghaemmaghami, A.M.; Ziaie, B. A Janus-paper PDMS platform for air–liquid interface cell culture applications. J. Micromech. Microeng. 2015, 25, 055015. [Google Scholar] [CrossRef] [Green Version]
- Mahadik, B.P.; Haba, S.P.; Skertich, L.J.; Harley, B.A. The use of covalently immobilized stem cell factor to selectively affect hematopoietic stem cell activity within a gelatin hydrogel. Biomaterials 2015, 67, 297–307. [Google Scholar] [CrossRef] [Green Version]
- Wang, H.; Liu, H.; Liu, H.; Su, W.; Chen, W.; Qin, J. One-Step Generation of Core–Shell Gelatin Methacrylate (GelMA) Microgels Using a Droplet Microfluidic System. Adv. Mater. Technol. 2019, 4, 1800632. [Google Scholar] [CrossRef]
- Cui, J.; Wang, H.; Shi, Q.; Ferraro, P.; Sun, T.; Dario, P.; Huang, Q.; Fukuda, T. Permeable hollow 3D tissue-like constructs engineered by on-chip hydrodynamic-driven assembly of multicellular hierarchical micromodules. Acta Biomater. 2020, 113, 328–338. [Google Scholar] [CrossRef]
- Cui, J.; Wang, H.; Shi, Q.; Sun, T.; Huang, Q.; Fukuda, T. Multicellular Co-Culture in Three-Dimensional Gelatin Methacryloyl Hydrogels for Liver Tissue Engineering. Molecules 2019, 24, 1762. [Google Scholar] [CrossRef] [Green Version]
- Sheikhi, A.; De Rutte, J.; Haghniaz, R.; Akouissi, O.; Sohrabi, A.; Di Carlo, D.; Khademhosseini, A. Microfluidic-enabled bottom-up hydrogels from annealable naturally-derived protein microbeads. Biomaterials 2019, 192, 560–568. [Google Scholar] [CrossRef]
- Wang, J.; Chen, G.; Zhao, Z.; Sun, L.; Zou, M.; Ren, J.; Zhao, Y. Responsive graphene oxide hydrogel microcarriers for controllable cell capture and release. Sci. China Mater. 2018, 61, 1314–1324. [Google Scholar] [CrossRef] [Green Version]
- Nie, J.; Gao, Q.; Wang, Y.; Zeng, J.; Zhao, H.; Sun, Y.; Shen, J.; Ramezani, H.; Fu, Z.; Liu, Z.; et al. Vessel-on-a-chip with Hydrogel-based Microfluidics. Small 2018, 14, e1802368. [Google Scholar] [CrossRef]
- Azizipour, N.; Avazpour, R.; Rosenzweig, D.H.; Sawan, M.; Ajji, A. Evolution of Biochip Technology: A Review from Lab-on-a-Chip to Organ-on-a-Chip. Micromachines 2020, 11, 599. [Google Scholar] [CrossRef] [PubMed]
- Zhang, Y.S.; Davoudi, F.; Walch, P.; Manbachi, A.; Luo, X.; Dell’Erba, V.; Miri, A.K.; Albadawi, H.; Arneri, A.; Li, X.; et al. Bioprinted thrombosis-on-a-chip. Lab Chip 2016, 16, 4097–4105. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Li, L.; Chen, Z.; Shao, C.; Sun, L.; Zhao, Y. Graphene Hybrid Anisotropic Structural Color Film for Cardiomyocytes’ Monitoring. Adv. Funct. Mater. 2020, 30, 1906353. [Google Scholar] [CrossRef]
- Wang, Y.; Wu, D.; Wu, G.; Wu, J.; Lu, S.; Lo, J.; He, Y.; Zhao, C.; Zhao, X.; Zhang, H.; et al. Metastasis-on-a-chip mimicking the progression of kidney cancer in the liver for predicting treatment efficacy. Theranostics 2020, 10, 300–311. [Google Scholar] [CrossRef] [PubMed]
- Aung, A.; Theprungsirikul, J.; Lim, H.L.; Varghese, S. Chemotaxis-driven assembly of endothelial barrier in a tumor-on-a-chip platform. Lab Chip 2016, 16, 1886–1898. [Google Scholar] [CrossRef] [Green Version]
- Roberts, S.A.; DiVito, K.A.; Ligler, F.S.; Adams, A.A.; Daniele, M.A. Microvessel manifold for perfusion and media exchange in three-dimensional cell cultures. Biomicrofluidics 2016, 10, 054109. [Google Scholar] [CrossRef] [Green Version]
- Nan, L.; Yang, Z.; Lyu, H.; Lau, K.Y.Y.; Shum, H.C. A Microfluidic System for One-Chip Harvesting of Single-Cell-Laden Hydrogels in Culture Medium. Adv. Biosyst. 2019, 3, 1900076. [Google Scholar] [CrossRef]
- Hu, X.; Zhao, S.; Luo, Z.; Zuo, Y.F.; Wang, F.; Zhu, J.; Chen, L.; Yang, D.; Zheng, Y.; Zheng, Y.; et al. On-chip hydrogel arrays individually encapsulating acoustic formed multicellular aggregates for high throughput drug testing. Lab Chip 2020, 20, 2228–2236. [Google Scholar] [CrossRef]
Hydrogels | Device | Cell Type | Aims and Achievements | Ref. |
---|---|---|---|---|
GelMA | 3-D microfluidic device, consisting of five microchambers and four bridge microchannels | Neural stem cells (NSCs) and tumors | Cell co-culture in a 3-D manner | [33] |
GelMA, Methyl cellulose, and mineral oil | Droplet flow-focusing microfluidic device | Hepatocytes (HepG2) cells and human umbilical vein endothelial cells (HUVECs) | Core–shell architectures and heterogenous cell cultures | [87] |
PEGDA/GelMA | Pressure—assisted hydrodynamic—driven assembly microfluidic chip | HepG2 and HUVECs | Albumin secretion of embedded cells | [88] |
GelMA | Digital micromirror device (DMD)-based microfluidic channel | Hepatocytes and fibroblasts | Layered cellular micromodules | [89] |
GelMA | Flow focusing microfluidic device | NIH/3T3 fibroblasts | Self-standing microporous environment with an orthogonal void fraction and stiffness | [90] |
GO, poly (N-isopropylacrylamide) (pNIPAM) andGelMA | Capillary | HepG2 cells and Hepa1-6 cells | Controllable cell capture and release | [91] |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Share and Cite
Liu, T.; Weng, W.; Zhang, Y.; Sun, X.; Yang, H. Applications of Gelatin Methacryloyl (GelMA) Hydrogels in Microfluidic Technique-Assisted Tissue Engineering. Molecules 2020, 25, 5305. https://doi.org/10.3390/molecules25225305
Liu T, Weng W, Zhang Y, Sun X, Yang H. Applications of Gelatin Methacryloyl (GelMA) Hydrogels in Microfluidic Technique-Assisted Tissue Engineering. Molecules. 2020; 25(22):5305. https://doi.org/10.3390/molecules25225305
Chicago/Turabian StyleLiu, Taotao, Wenxian Weng, Yuzhuo Zhang, Xiaoting Sun, and Huazhe Yang. 2020. "Applications of Gelatin Methacryloyl (GelMA) Hydrogels in Microfluidic Technique-Assisted Tissue Engineering" Molecules 25, no. 22: 5305. https://doi.org/10.3390/molecules25225305